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Recombinant DNA and Gene Cloning
Recombinant DNA and Gene Cloning

... Cloning other Genes The recombinant vector described above could itself be a useful tool for cloning other genes. Let us assume that within its kanamycin resistance gene (kanr) there is a single occurrence of the sequence 5' GAATTC 3' 3' CTTAAG 5' This is cut by the restriction enzyme EcoRI, produci ...
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Mutation and DNA Repair
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... 1. A large number of sperm cells are produced by males every day. This large number of sperm cells increases the chance that (1) at least one sperm cell will be reached when the eggs swim toward the sperm cells in the ovary (2) several sperm cells will unite with an egg so the fertilized egg will de ...
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Vectors in gene therapy

Gene therapy utilizes the delivery of DNA into cells, which can be accomplished by several methods, summarized below. The two major classes of methods are those that use recombinant viruses (sometimes called biological nanoparticles or viral vectors) and those that use naked DNA or DNA complexes (non-viral methods).
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