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P S .D. Marshall S. Horwitz, M.D. Faculty Prize
P S .D. Marshall S. Horwitz, M.D. Faculty Prize

... biology of the adenovirus, first by describing the outer protein of the virus and then by developing a method for replicating its DNA. These findings have proven crucial in offering researchers a system for studying adenovirus biology and using it in gene therapy. He explored how adenovirus genes he ...
cDNA cloning, expression and chromosomal localization of the
cDNA cloning, expression and chromosomal localization of the

... Trx1 gene. We designed primers flanking the homology region (Forward 5´GGCTTGTGCTGGGATAGAGCTG-3´ and reverse 5´-CCCACACACACATACAC ATCCCC-3´) and amplified by PCR a fragment from human genomic DNA (Clontech). We cloned the fragment in pGEM-Teasy vector and sequenced it in both directions confirming ...
Biology End-of-Course Test: Heritage High School 2013
Biology End-of-Course Test: Heritage High School 2013

... ii. Celllose: found in cell walls of plants & used for structural support iii. Glycogen : energy storage for animals 2) Protein Examples a. Hemoglobin : used to transport oxygen through the blood b. Insulin : used to regulate amount of glucose in blood c. Enzyme: used as a catalyst to speed up chemi ...
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... Developing suitable methods for locating and isolating genes of interest is an important part of gene technology There are three main methods for obtaining genes • Synthesising the gene using an automated gene machine – this method can be used if the amino sequence of the protein gene product is kno ...
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PH_Genetics__Natural..

...  The mRNA carries the code from the nucleus to the ribosomes in the cytoplasm.  RNA is a single-stranded polymer of four nucleotide monomers. A RNA nucleotide is identified by the base it contains-adenine, guinine, cyctosine or uracil.  At the ribosome, amino acids are linked to form specific pro ...
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STAAR REVIEW - TEKS BASED

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Aim: What is positive feedback of bacterial operons?

... pathways, synthesizing end products. (tryptophan synthesis).  Inducible enzymes usually function in catabolic pathways, digesting nutrients to simpler molecules. (lactose metabolism).  Both repressible and inducible operons demonstrate negative control because active repressors can only have negat ...
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... that PCR product should be free of non-specific amplification products that can interfere with transcription and/or translation. A tag (e.g. His-tag) can be added to the DNA template to facilitate protein detection and purification. ...
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... the cycle is driven by cyclins activated by cell division cycle kinases (Cdks), which in turn have been activated by regulatory genes, such as myc. Expression of positive-acting regulatory genes, such as myc, is induced by cytoplasmic signals initiated by receptor kinase following interaction with a ...
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... Human DNA • There are about 3bn (3  109) nucleotides in the nucleus of almost all of the trillions (3.5  1012 ) of cells of a human body (an exception is, for example, red blood cells which have no nucleus and therefore no DNA) – a total of ~1022 nucleotides! • Many DNA regions code for proteins, ...
Exam 4 Review - Iowa State University
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DNA bracelet activity pack
DNA bracelet activity pack

... The best way to gauge understanding of participants is to ask them questions like »» Do you think everyone has the same colour eyes or hair? »» What colour are your eyes? Note that we don’t all have the same colour of eyes or hair because what we look like depends on information that we inherit from ...
Unit 7a * Structure of DNA
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... simultaneously with DNA and with polymerase and so recruits the enzyme to the promoter. • Allostery is not only a mechanism of gene activation, it is also often the way regulators are controlled by their specific signals. • A typical bacterial regulator can adopt two conformations- in one it can bin ...
Midas_2 - PhagesDB
Midas_2 - PhagesDB

... Gene 7: I moved the start codon from 4460 bp to 4409bp in order to make it the longest reading frame with a start codon of ATG. Gene 20: I moved the start codon to 14325 instead of the 14415, which provided a longer reading frame and when re-blasted also gave me a tail assembly chaperone function. I ...
CRISPR/Cas9.
CRISPR/Cas9.

... protein 9) 3. What general biological, chemical, and/or physical principles and concepts is this technique based on? The CRISPR/Cas system is a bacterial and archaeal adaptive immune defense system that cleaves foreign genetic material [1]. The CRISPR locus within the prokaryotic genome has short re ...
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Vectors in gene therapy

Gene therapy utilizes the delivery of DNA into cells, which can be accomplished by several methods, summarized below. The two major classes of methods are those that use recombinant viruses (sometimes called biological nanoparticles or viral vectors) and those that use naked DNA or DNA complexes (non-viral methods).
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