Significance of bacterial identification by molecular
... randomly select a proportion of the amplification products (10–20%) and subject them to comparative sequence analysis, i.e. identify them. A further consideration is the detection limit of the particular PCR technique (this may vary with user, reagents and equipment). It must always be borne in mind ...
... randomly select a proportion of the amplification products (10–20%) and subject them to comparative sequence analysis, i.e. identify them. A further consideration is the detection limit of the particular PCR technique (this may vary with user, reagents and equipment). It must always be borne in mind ...
Target selected insertional mutagenesis on chromosome IV of
... Fig. 1. Schematic representation of three-dimensional pooling and PCR strategies. A small population of 960 I element containing Arabidopsis lines was divided over 10 blocks (trays) and each block contained 96 plants (8 rows and 12 columns). Inflorescence material of every plant in the population wa ...
... Fig. 1. Schematic representation of three-dimensional pooling and PCR strategies. A small population of 960 I element containing Arabidopsis lines was divided over 10 blocks (trays) and each block contained 96 plants (8 rows and 12 columns). Inflorescence material of every plant in the population wa ...
Transcripts of the MHM region on the chicken Z chromosome
... Recently, the ASW/Wpkci gene was found in the distal non-hetrochromatic region on the short arm of the chicken W chromosome (O'Neill et al. 2000, Hori et al. 2000). Wpkci is actively transcribed during the early embryonic stage and encodes an altered form of PKCI (protein kinase C interacting protei ...
... Recently, the ASW/Wpkci gene was found in the distal non-hetrochromatic region on the short arm of the chicken W chromosome (O'Neill et al. 2000, Hori et al. 2000). Wpkci is actively transcribed during the early embryonic stage and encodes an altered form of PKCI (protein kinase C interacting protei ...
Antisense derivatives of U7 small nuclear RNA as
... To be effective, splicing-modulating antisense RNAs must accumulate in the nucleoplasm where splicing occurs (see chapter 42 Aartsma Rus). This is why derivatives of U small nuclear RNAs (snRNAs), and in particular of U7 snRNA, have been widely used for this purpose [1]. Apart from the advantage tha ...
... To be effective, splicing-modulating antisense RNAs must accumulate in the nucleoplasm where splicing occurs (see chapter 42 Aartsma Rus). This is why derivatives of U small nuclear RNAs (snRNAs), and in particular of U7 snRNA, have been widely used for this purpose [1]. Apart from the advantage tha ...
MayerFrankiPoster
... closest to the surface absorb most of the sunlight and prevent the cells beneath from receiving sufficient light. This is known as ‘overshadowing.’ As a result cultures are not able to grow to high densities with high bioproduct yields. My goal is to inactivate the cpcB gene by inserting an antibiot ...
... closest to the surface absorb most of the sunlight and prevent the cells beneath from receiving sufficient light. This is known as ‘overshadowing.’ As a result cultures are not able to grow to high densities with high bioproduct yields. My goal is to inactivate the cpcB gene by inserting an antibiot ...
Biosafety Protocol Registration Form
... D-4: Experiments involving whole animals in which the animal’s genome has been altered by stable introduction of recombinant DNA, or DNA derived therefrom, into the germ-line (transgenic animals), and experiments involving viable recombinant DNA-modified microorganisms tested on whole animals. For t ...
... D-4: Experiments involving whole animals in which the animal’s genome has been altered by stable introduction of recombinant DNA, or DNA derived therefrom, into the germ-line (transgenic animals), and experiments involving viable recombinant DNA-modified microorganisms tested on whole animals. For t ...
Correlations Between Gene Expression and Gene Conservation in
... To extend this observation, we compared the distribution of the three gene categories among genes regulated during sexual differentiation (Mata et al. 2002). Unlike the comparisons in Figure 1, the following analyses do not consider absolute levels of gene expression, but are based on relative expre ...
... To extend this observation, we compared the distribution of the three gene categories among genes regulated during sexual differentiation (Mata et al. 2002). Unlike the comparisons in Figure 1, the following analyses do not consider absolute levels of gene expression, but are based on relative expre ...
Methods for detection of point mutations
... Limit of detection. Approximately one mutant cell is detectable in the presence of 10 normal cells [22]. Methods of detection. Initially, fragments were labeled radioactively and detected by autoradiography. Nonradioactive detection, e.g., by silver staining, is feasible. Performance and quality ass ...
... Limit of detection. Approximately one mutant cell is detectable in the presence of 10 normal cells [22]. Methods of detection. Initially, fragments were labeled radioactively and detected by autoradiography. Nonradioactive detection, e.g., by silver staining, is feasible. Performance and quality ass ...
Leukaemia Section inv(3)(q21q26)x2 Atlas of Genetics and Cytogenetics in Oncology and Haematology
... hematopoietic stem cells and can inhibit myeloid differentiation. Two alternative forms exists, one generated from EVI1, the other MECOM (MDS1 and EVI1 complex locus) through intergenic splicing with MDS1 (myelodysplasia syndrome 1), a gene located 140 kb upstream of EVI1. DNA/RNA EVI1 has 16 exons, ...
... hematopoietic stem cells and can inhibit myeloid differentiation. Two alternative forms exists, one generated from EVI1, the other MECOM (MDS1 and EVI1 complex locus) through intergenic splicing with MDS1 (myelodysplasia syndrome 1), a gene located 140 kb upstream of EVI1. DNA/RNA EVI1 has 16 exons, ...
Contribution of IKBKE and IFIH1 gene variants to SLE susceptibility
... Figure 1. The IKBKE SNV rs12142086 affects the binding of SF1 with DNA. (a) The SNV rs12142086 is predicted to be located in the binding motif of SF1. Introns are represented by grey horizontal lines and exons by vertical bars with numbers. The position of rs12142086 is highlighted with a star. In t ...
... Figure 1. The IKBKE SNV rs12142086 affects the binding of SF1 with DNA. (a) The SNV rs12142086 is predicted to be located in the binding motif of SF1. Introns are represented by grey horizontal lines and exons by vertical bars with numbers. The position of rs12142086 is highlighted with a star. In t ...
LYTIC AND LYSOGENIC CYCLES
... c. Repression of the phage genome - A phage coded protein, called a repressor, is made which binds to a particular site on the phage DNA, called the operator, and shuts off transcription of most phage genes EXCEPT the repressor gene. The result is a stable repressed phage genome which is integrated ...
... c. Repression of the phage genome - A phage coded protein, called a repressor, is made which binds to a particular site on the phage DNA, called the operator, and shuts off transcription of most phage genes EXCEPT the repressor gene. The result is a stable repressed phage genome which is integrated ...
Evolutionary Origin of Recombination during Meiosis
... bacterial cell, whereby nonsister homologous chromosomes (or parts of chromosomes) originating from different parent cells align and undergo genetic recombination. The recombined DNA is passed on to a progeny bacterium. The core genes for meiosis were very likely present in the common ancestor of al ...
... bacterial cell, whereby nonsister homologous chromosomes (or parts of chromosomes) originating from different parent cells align and undergo genetic recombination. The recombined DNA is passed on to a progeny bacterium. The core genes for meiosis were very likely present in the common ancestor of al ...
Alu electrophoresis PCR lab
... DNA Ladder consists of known DNA sizes used to determine the size of an unknown DNA sample. The DNA ladder usually contains regularly spaced sized samples which when run on an agarose gel looks like a "ladder". ...
... DNA Ladder consists of known DNA sizes used to determine the size of an unknown DNA sample. The DNA ladder usually contains regularly spaced sized samples which when run on an agarose gel looks like a "ladder". ...
Biology OF CLONING VECTORS-III-B.SC - E
... phenotype is readily scorable, for in this way it is possible to distinguish chimeras from cleaved plasmid molecules which have self-annealed. Of course, readily detectable insertional inactivation is not essential if the vector and insert are to be joined by the hormopolymer tailing method or tif t ...
... phenotype is readily scorable, for in this way it is possible to distinguish chimeras from cleaved plasmid molecules which have self-annealed. Of course, readily detectable insertional inactivation is not essential if the vector and insert are to be joined by the hormopolymer tailing method or tif t ...
Antisense derivatives of U7 small nuclear RNA as
... To be effective, splicing-modulating antisense RNAs must accumulate in the nucleoplasm where splicing occurs. This is why derivatives of U small nuclear RNAs (snRNAs), and in particular of U7 snRNA, have been widely used for this purpose [1]. Apart from the advantage that the antisense RNA accumulat ...
... To be effective, splicing-modulating antisense RNAs must accumulate in the nucleoplasm where splicing occurs. This is why derivatives of U small nuclear RNAs (snRNAs), and in particular of U7 snRNA, have been widely used for this purpose [1]. Apart from the advantage that the antisense RNA accumulat ...
Hierarchical DNA Sequencing
... Break genome into many long pieces Map each long piece onto the genome Sequence each piece with shotgun ...
... Break genome into many long pieces Map each long piece onto the genome Sequence each piece with shotgun ...
DNA-binding proteins
... • Enzyme induction can also be controlled by a repressor • Addition of inducer inactivates a repressor protein (not same as corepressor substance) , and transcription can proceed (Figure 7.8) ...
... • Enzyme induction can also be controlled by a repressor • Addition of inducer inactivates a repressor protein (not same as corepressor substance) , and transcription can proceed (Figure 7.8) ...
bioinformatics
... ancestor. Two such segments are called segmental homologs (SH). When dealing with an incompletely mapped genome, knowing that two segments are homologous is useful in that it suggests that other (unmapped) features within those same segments may have homologous counterparts in the opposite segment. ...
... ancestor. Two such segments are called segmental homologs (SH). When dealing with an incompletely mapped genome, knowing that two segments are homologous is useful in that it suggests that other (unmapped) features within those same segments may have homologous counterparts in the opposite segment. ...
Mechanisms of fast and stringent search in homologous pairing of
... PH, and shear force, suggesting that it may serve as the ‘default’ mode of chromosome pairing in vivo [2]. Various models have been proposed to explain the homology-dependent attraction between dsDNA molecules [9–11], many of which attribute this interaction to hydrophobic forces or electrostatics. ...
... PH, and shear force, suggesting that it may serve as the ‘default’ mode of chromosome pairing in vivo [2]. Various models have been proposed to explain the homology-dependent attraction between dsDNA molecules [9–11], many of which attribute this interaction to hydrophobic forces or electrostatics. ...
Genomic surveys and expression analysis of bZIP gene family in
... and oligo (dT) used as the primers to synthesize the first and second-strand cDNA. The cDNA was digested by two types of endonuclease NlaIII or DpnII, acquiring 17 bp tags with different adaptors of both ends to generate a tag library. After 15 cycles of linear PCR amplification, 105 bp fragments we ...
... and oligo (dT) used as the primers to synthesize the first and second-strand cDNA. The cDNA was digested by two types of endonuclease NlaIII or DpnII, acquiring 17 bp tags with different adaptors of both ends to generate a tag library. After 15 cycles of linear PCR amplification, 105 bp fragments we ...
Comprehensive Analysis of Hyrdrogen Bonds in Regulatory Protein
... DNA backbone. Interactions that involve atoms in the protein backbone were found predominantly with the DNA backbone rather than with the base edges. The scarcity of Pbb × Dbe contacts (nine only) is probably due to conformational restrictions that prevent their formation. Most of the interactions w ...
... DNA backbone. Interactions that involve atoms in the protein backbone were found predominantly with the DNA backbone rather than with the base edges. The scarcity of Pbb × Dbe contacts (nine only) is probably due to conformational restrictions that prevent their formation. Most of the interactions w ...
Tandem and segmental gene duplication and
... clusters, respectively, contained genes from different clades. Seven heterogeneous clusters were interpreted as the amplification products of an ancient two-gene module [8,9] (Figure 1). For the remaining four clusters, two origins were suggested: (i) association of NBS-LRR genes from different clad ...
... clusters, respectively, contained genes from different clades. Seven heterogeneous clusters were interpreted as the amplification products of an ancient two-gene module [8,9] (Figure 1). For the remaining four clusters, two origins were suggested: (i) association of NBS-LRR genes from different clad ...