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News in DNA/RNA electrophoresis: Midori
News in DNA/RNA electrophoresis: Midori

... revertant colonies of strains TA98 and TA102 were increased, but did not exceed as twice as those of the spontaneous revertant colonies, and there was no dose-response relationship. Appropriate reference mutagens were used as positive controls and they showed a distinct increase of induced revertant ...
summary slides
summary slides

... Species defined Eukaryotic species: A group of closely related organisms that breed among themselves Prokaryotic species: A population of cells with similar characteristics Clone: A population of cells derived from a single cell Strain: A subgroup within a species with one or more characteristics t ...
Cloning the Progesterone 5 beta- reductase gene
Cloning the Progesterone 5 beta- reductase gene

... We soon found that the Butterfly we chose (the Monarch) does not actually produce the toxin itself but rather sequesters it from its food source, the Milkweed, as a larvae and pupa. The milkweed itself did not have a gene on file that we could isolate so we did some research on what exactly this tox ...
DNA: The molecular basis of mutations
DNA: The molecular basis of mutations

... 1. No change occurs in phenotype. Some mutations don't have any noticeable effect on the phenotype of an organism. This can happen in many situations: perhaps the mutation occurs in a stretch of DNA with no function, or perhaps the mutation occurs in a protein-coding region, but ends up not affectin ...
Transcription
Transcription

... RNA is known as transcription (TC), with the further conversion into protein being termed translation (TL). This concept of information flow is known as the Central Dogma of molecular biology and is an underlying theme in all studies of gene expression. ...
Genetic Engineering
Genetic Engineering

... What is cloning? • Reproductive cloning – Making an exact copy of a pre-existing or currently existing organism. • Therapeutic cloning – Embryos are grown and stem cells from the embryo are harvested to be used in research to develop treatment for cancer, Alzheimer’s, etc. – Very controversial b/c ...
Chapter 2 Human Genetics Overview The purpose of this chapter is
Chapter 2 Human Genetics Overview The purpose of this chapter is

... Chromosomes and genes o The nuclear DNA is bound together in long strands called chromosomes. o The number of chromosomes varies by species. Humans have 46 (23 pairs) while chimpanzees and gorillas have 48 (24 pairs) o About 10 million years ago human ancestral DNA fused two chromosomes together int ...
Biotechnology - BHSBiology-Cox
Biotechnology - BHSBiology-Cox

... • Scientists began experimenting with molecules, cells, tissues, and organs (moving away from entire organisms). WHY? • New technologies are applied to the research and development of products from plant and animal tissues ...
Slide 1
Slide 1

... • Transcription – DNA makes a copy of itself that can leave the nucleus. This copy is called messenger RNA (mRNA). It is exactly the same as DNA except for one thing; instead of the nitrogenous base thymine, all RNA has the nitrogenous base uracil (U). • The newly created mRNA then leaves the nucleu ...
The fate of transgenes in the human gut
The fate of transgenes in the human gut

Slayt 1
Slayt 1

... Some phages can transfer only particular genes to other bacteria.  Phage lambda (λ) has this property. To understand specialized transduction, we need to examine the phage lambda life cycle.  lambda has 2 distinct phases of its life cycle. The “lytic” phase is the same as we saw with the general p ...
Gene to protein
Gene to protein

... TRANSLATION = RNA → PROTEINS (Occurs on RIBOSOMES in CYTOPLASM in both PROKARYOTES & EUKARYOTES) Specific AMINO ACYL tRNA SYNTHETASES added amino acids to correct tRNA’s TRANSLATION & TRANSCRIPTION happen simultaneously in PROKARYOTES ...
DNA and Evolution
DNA and Evolution

... Two basic types: those that are strictly DNA, and those that replicate through an RNA intermediate. Most bacterial TEs are DNA only Most common type: Insertion Sequences (IS) – roughly 1-3 kbp long, containing a transposase gene, and are bounded by short (10-40 bp) inverted repeats – many different ...
Ch 1617 Study Guide - Dublin City Schools
Ch 1617 Study Guide - Dublin City Schools

... TRANSLATION = RNA → PROTEINS (Occurs on RIBOSOMES in CYTOPLASM in both PROKARYOTES & EUKARYOTES) Specific AMINO ACYL tRNA SYNTHETASES added amino acids to correct tRNA’s TRANSLATION & TRANSCRIPTION happen simultaneously in PROKARYOTES ...
Identical Versus Fraternal Twins
Identical Versus Fraternal Twins

... = the complete instructions for making an organism, consisting of all the genetic material in that organism’s chromosomes. ...
2 - UPCH
2 - UPCH

Mitochondrial DNA - MrsWrightsSciencePage
Mitochondrial DNA - MrsWrightsSciencePage

... PCR has allowed us to map segments of the Human Genome that code for rare diseases… this allows us to do genetic testing – on infants and on you! this also allows you to be able to find out if you have the gene for Alzheimer's Disease, or Parkinson’s Disease or Cystic Fibrosis or many ...
11_DNA is the genetic material (MRU)
11_DNA is the genetic material (MRU)

... populations of species as they change from one generation to the next, over time, and in different environments. Heredity is the concept that the characteristics of an individual plant or animal in a population could be passed down through the generations. Offspring look more ...
Name: Date: Period:___ Midterm Review: Study Guide # 4 TOPICS
Name: Date: Period:___ Midterm Review: Study Guide # 4 TOPICS

... wolves, and owls. Which trait is more likely to be inherited by the new generation of hares: white or black fur? Why? ...
II. Conversion Tables and Formulas
II. Conversion Tables and Formulas

... Store RNA at –70° to –80°C, as aliquots in ethanol or isopropanol. Most RNA is relatively stable at this temperature. Centrifuge the RNA and resuspend in the appropriate RNase-free buffer before use. Drying, dissolving and pipetting RNA ...
5. Nucleic Acids-Structure, Central Dogma – Bio 20
5. Nucleic Acids-Structure, Central Dogma – Bio 20

... domains, three capped by loops, one by a stem ...
topic 5 : expression of biological information
topic 5 : expression of biological information

... A. the ability to form complementary base pairs with other DNA nucleotides. B. the ability to form complementary base pairs with RNA nucleotides C. histone proteins associated with the double helix. D. a sequence of nucleotides that can be decoded into a sequence of amino acids in a protein. ...
What are mutations and how do they affect the production
What are mutations and how do they affect the production

... The enzymes responsible for matching up the nucleotide bases can match the ______________ bases together, ___________ a base out, or __________ an extra base. This occurs during DNA ______________ ...
Evidence of evolution
Evidence of evolution

... ▪ in most cases, evidence from DNA and protein structures have just confirmed what we concluded from fossils, embryos, and body structure. ▪ Sometimes scientists have been corrected by DNA evidence. For example: scientist thought the lesser pandas were closely related to the giant panda. However, DN ...
Protein Synthesis (Transcription and Translation)
Protein Synthesis (Transcription and Translation)

... nucleotides in each gene contains information for assembling the string of amino acids that make up ...
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Non-coding DNA

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