Analysis of Guanine Oxidation Products in Double
... and can form several oxidation products (Figure 1). Guanine-rich sequences exist in many important genomic regions, such as telomeres [10–13] and the promoter element of the proto-oncogene c-myc [14,15], and these sequences can fold into quadruplex structures in the presence of suitable metal ions. ...
... and can form several oxidation products (Figure 1). Guanine-rich sequences exist in many important genomic regions, such as telomeres [10–13] and the promoter element of the proto-oncogene c-myc [14,15], and these sequences can fold into quadruplex structures in the presence of suitable metal ions. ...
The eukaryotic genome: a system regulated at different hierarchical
... identified in a variety of loci (Li et al., 1999; Li et al., 2002). Activation of a genomic domain by such control elements is necessary for activation of individual genes in the cluster but may not be sufficient. The second class of element comprises the enhancers and promoters that decide the acti ...
... identified in a variety of loci (Li et al., 1999; Li et al., 2002). Activation of a genomic domain by such control elements is necessary for activation of individual genes in the cluster but may not be sufficient. The second class of element comprises the enhancers and promoters that decide the acti ...
The Mouse Immunoglobulin Heavy Chain V
... With the exception of the intronic enhancer E, the regulatory elements that control ordered recombination and allelic exclusion have not been identified. E is required for efficient D to J recombination (18, 19). It acts in part by activation of antisense intergenic transcription, which is abrogat ...
... With the exception of the intronic enhancer E, the regulatory elements that control ordered recombination and allelic exclusion have not been identified. E is required for efficient D to J recombination (18, 19). It acts in part by activation of antisense intergenic transcription, which is abrogat ...
DROSOPHILA MELANOGASTER - THE MODEL ORGANISM OF
... mutations. P elements, by inserting themselves into the genomic DNA, naturally act as mutagens. In order to be able to determine very rapidly which gene is affected by a particular P element insertion, versions of P elements that permit the rapid and simple cloning of the DNA adjacent to their inser ...
... mutations. P elements, by inserting themselves into the genomic DNA, naturally act as mutagens. In order to be able to determine very rapidly which gene is affected by a particular P element insertion, versions of P elements that permit the rapid and simple cloning of the DNA adjacent to their inser ...
Spider Silk - Consortium for Mathematics and its Applications
... To use the table, start with any DNA nucleotide triplet (for example CCT). Transcribe this to a mRNA codon by replacing all the Ts with Us (to get CCU). Read the triplet from left to right (first letter C, second letter C, third letter U) and follow along in the table. You should arrive at the box c ...
... To use the table, start with any DNA nucleotide triplet (for example CCT). Transcribe this to a mRNA codon by replacing all the Ts with Us (to get CCU). Read the triplet from left to right (first letter C, second letter C, third letter U) and follow along in the table. You should arrive at the box c ...
Severe oligozoospermia resulting from deletions
... produced Y-bearing, AZF-deleted spermatozoa. We had previously suggested4 that AZF normally influences the fate of spermatogonial stem cells or facilitates the differentiation of their precursors, the primordial germ cells. The present findings are consistent with, but do not provide definitive evid ...
... produced Y-bearing, AZF-deleted spermatozoa. We had previously suggested4 that AZF normally influences the fate of spermatogonial stem cells or facilitates the differentiation of their precursors, the primordial germ cells. The present findings are consistent with, but do not provide definitive evid ...
Frequent, independent transfers of a catabolic gene from bacteria to
... Indeed, gene duplications (followed by evolution of new functions) and other genomic rearrangements have shaped eukaryotic genomes [1]. However, genetic innovation can also result from the acquisition of exogenous genes by horizontal gene transfer (HGT). Prokaryotes adapt largely by HGT, and strains ...
... Indeed, gene duplications (followed by evolution of new functions) and other genomic rearrangements have shaped eukaryotic genomes [1]. However, genetic innovation can also result from the acquisition of exogenous genes by horizontal gene transfer (HGT). Prokaryotes adapt largely by HGT, and strains ...
A simple centrifugation protocol leads to a 55-fold
... 1. DNA-based methods are increasingly used to study and monitor biodiversity and DNA (meta)barcoding is readily available for standardised assessments. However, (meta)barcoding suffers from bias introduced by PCR and the use of primers, which can lead to the loss of taxonomic groups. PCR-free techni ...
... 1. DNA-based methods are increasingly used to study and monitor biodiversity and DNA (meta)barcoding is readily available for standardised assessments. However, (meta)barcoding suffers from bias introduced by PCR and the use of primers, which can lead to the loss of taxonomic groups. PCR-free techni ...
Physical Mapping of a 670-kb Region of Chromosomes XVI and XVII
... amounts of exonuclease Bal31 following digestion with SfiI restriction enzyme (Fig. 3). The loci h49 were located in two SfiI fragments of 350 kb and 450 kb that were sensitive to the treatment with Bal31. On the other hand, JL8 probe reacted with two SfiI fragments (50 and 23 kb, note that there is ...
... amounts of exonuclease Bal31 following digestion with SfiI restriction enzyme (Fig. 3). The loci h49 were located in two SfiI fragments of 350 kb and 450 kb that were sensitive to the treatment with Bal31. On the other hand, JL8 probe reacted with two SfiI fragments (50 and 23 kb, note that there is ...
CHAPTER 15 THE CHROMOSOMAL BASIS OF INHERITANCE
... Whether an embryo develops into a male or female depends upon the presence of a Y chromosome. • A British research team has identified a gene, SRY (sex-determining region of Y), on the Y chromosome that is responsible for triggering the complex series of events that lead to normal testicular develop ...
... Whether an embryo develops into a male or female depends upon the presence of a Y chromosome. • A British research team has identified a gene, SRY (sex-determining region of Y), on the Y chromosome that is responsible for triggering the complex series of events that lead to normal testicular develop ...
Application of whole genome sequencing to fully characterise
... Campylobacter is the most common cause of acute bacterial gastroenteritis worldwide. In the UK alone it causes an estimated 500,000 infections each year. There have been two large studies of Infectious Intestinal Disease in the UK community (IID1 in the mid 1990s and IID2 in 2008-2009). In both stud ...
... Campylobacter is the most common cause of acute bacterial gastroenteritis worldwide. In the UK alone it causes an estimated 500,000 infections each year. There have been two large studies of Infectious Intestinal Disease in the UK community (IID1 in the mid 1990s and IID2 in 2008-2009). In both stud ...
Systematic Mutational Analysis of the Yeast ACT1 Gene.
... many potential binding and otherfunctional domains of the protein. Recently, powerful general techniques for systematic mutagenesis of protein coding sequenceshave beendeveloped. These were aimed in the firstinstance at in vitro biochemical studies of the proteins, for which it was desired to alter ...
... many potential binding and otherfunctional domains of the protein. Recently, powerful general techniques for systematic mutagenesis of protein coding sequenceshave beendeveloped. These were aimed in the firstinstance at in vitro biochemical studies of the proteins, for which it was desired to alter ...
Harnessing the CRISPR/Cas9 system to disrupt latent HIV
... ntegration of reverse transcribed viral DNA into the host cell genome is an essential step during the HIV-1 life cycle1. The integrated retroviral DNA is termed a provirus, which serves as the fundamental source of viral protein production. HIV-1 gene expression is regulated by LTR promoter and enha ...
... ntegration of reverse transcribed viral DNA into the host cell genome is an essential step during the HIV-1 life cycle1. The integrated retroviral DNA is termed a provirus, which serves as the fundamental source of viral protein production. HIV-1 gene expression is regulated by LTR promoter and enha ...
Mitochondrial Transcript Processing and Restoration of Male Fertility
... hybrid seed in a number of crops. The Texas or T-cytoplasmic male-sterile (cms-T) system was used extensively in the 1960s to eliminate the need for hand detasseling in hybrid maize production. As a consequence of the 1970 epidemic of southern corn leaf blight, cms-T is no longer widely used commerc ...
... hybrid seed in a number of crops. The Texas or T-cytoplasmic male-sterile (cms-T) system was used extensively in the 1960s to eliminate the need for hand detasseling in hybrid maize production. As a consequence of the 1970 epidemic of southern corn leaf blight, cms-T is no longer widely used commerc ...
the art and design of genetic screens
... Box 2 | Use of transposons and genomics to search for mutants Transposon mutagenesis is commonly used for increasing the effectiveness of mutant screens. A wide range of transposon derivatives and delivery vectors are available (for example, see REF. 46). Similar to insertion sequence (IS) elements, ...
... Box 2 | Use of transposons and genomics to search for mutants Transposon mutagenesis is commonly used for increasing the effectiveness of mutant screens. A wide range of transposon derivatives and delivery vectors are available (for example, see REF. 46). Similar to insertion sequence (IS) elements, ...
Comparative sequence analysis of the long repeat regions and
... recognized in R L, encoding the immediate early transcriptional regulatory protein IE 110 or ICP0; this gene is flanked by substantial sequences the roles of which were less well defined. Downstream of the IE110 gene is a region of some 3500 bp which has not been assigned any protein coding function ...
... recognized in R L, encoding the immediate early transcriptional regulatory protein IE 110 or ICP0; this gene is flanked by substantial sequences the roles of which were less well defined. Downstream of the IE110 gene is a region of some 3500 bp which has not been assigned any protein coding function ...
Trawling DNA Databases for Partial Matches: What is the FBI Afraid
... product of a cognitive fallacy involving certain probabilities. Part III reviews the existing research and describes what it indicates about RMPs. It concludes that current research does not seriously undermine the vanishingly small theoretical estimates of RMPs. Part IV considers whether existing l ...
... product of a cognitive fallacy involving certain probabilities. Part III reviews the existing research and describes what it indicates about RMPs. It concludes that current research does not seriously undermine the vanishingly small theoretical estimates of RMPs. Part IV considers whether existing l ...
DNA phosphorothioation inStreptomyces lividans: mutational
... sylated [1-3]. Altered or unusual bases in DNA molecules often have significant physiological implications, such as DNA replication control, gene regulation, or protection of the respective organisms from invasion by foreign DNA [4]. Page 1 of 8 (page number not for citation purposes) ...
... sylated [1-3]. Altered or unusual bases in DNA molecules often have significant physiological implications, such as DNA replication control, gene regulation, or protection of the respective organisms from invasion by foreign DNA [4]. Page 1 of 8 (page number not for citation purposes) ...
Identification of markers tightly linked to tomato yellow
... defensive practices were utilized to control the disease after its rapid spread during the first outbreak, but the majority of these defensive methods are not effective. Therefore, breeding for resistance to tomato yellow leaf curl disease (TYLCD) is the most advantageous approach to controlling the ...
... defensive practices were utilized to control the disease after its rapid spread during the first outbreak, but the majority of these defensive methods are not effective. Therefore, breeding for resistance to tomato yellow leaf curl disease (TYLCD) is the most advantageous approach to controlling the ...
Sequence Information Encoded in DNA that May Influence Long
... completely lacked signals were also identified. These results are available in Table S2. Signal-rich clusters of size greater than 5 consecutive overlapping windows (300 kb) were allowed to contain one gap. For example: 5/6, 6/7, 7/8, etc. signals per group of overlapping 100 kb windows or better we ...
... completely lacked signals were also identified. These results are available in Table S2. Signal-rich clusters of size greater than 5 consecutive overlapping windows (300 kb) were allowed to contain one gap. For example: 5/6, 6/7, 7/8, etc. signals per group of overlapping 100 kb windows or better we ...
affinity chromatography of dna-binding proteins from human, murine
... cells eluting in peak I (Fig. 5 A). Similar patterns were obtained from the human line Daudi and from the mouse lines A9, A9HT and the mouse tumours Ehrlich, SEWA and MSWBS. The distribution of proteins derived from isolated HeLa nuclei prepared in aqueous solvents was compared with that of proteins ...
... cells eluting in peak I (Fig. 5 A). Similar patterns were obtained from the human line Daudi and from the mouse lines A9, A9HT and the mouse tumours Ehrlich, SEWA and MSWBS. The distribution of proteins derived from isolated HeLa nuclei prepared in aqueous solvents was compared with that of proteins ...
Recombinant DNA technology and molecular cloning
... can be isolated and amplified. One method of isolation and amplification of a gene of interest is to clone the gene by inserting it into another DNA molecule that serves as a vehicle or vector that can be replicated in living cells. When these two DNAs of different origin are combined, the result is ...
... can be isolated and amplified. One method of isolation and amplification of a gene of interest is to clone the gene by inserting it into another DNA molecule that serves as a vehicle or vector that can be replicated in living cells. When these two DNAs of different origin are combined, the result is ...