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Bios 560R: Introduction to Bioinformatics
Bios 560R: Introduction to Bioinformatics

... The cell is a complex machinery. The active parts of it are the proteins. The DNA records how each protein should be made, but not the quantity at a given moment. To understand the operation of the machinery, we want to know how much of each protein is present under certain conditions. There are pot ...
COAS_B1_Ch08 Nucleic acids
COAS_B1_Ch08 Nucleic acids

... are around 30 000 genes in our cells (Figure 8.7). The code is read in groups of three ‘letters’ – that is, triplets of bases. As we have seen, there are four bases in a DNA molecule, A, T, C and G. A sequence of three bases in a DNA molecule codes for one amino acid (Figure 8.8). ...
Chapter 8: From DNA to Proteins
Chapter 8: From DNA to Proteins

...  Franklin’s data gave Watson and Crick what they needed to ultimately figure out the structure of DNA. The Double Helix  Watson and Crick made many models using metal and wood to figure out the structure of DNA.  Their models put the sugar-phosphate backbones on the outside and the bases on the i ...
Infectious laryngotracheitis (ILT) is an acute and highly contagious
Infectious laryngotracheitis (ILT) is an acute and highly contagious

... comparison with the Serva genome showed that 35 open reading frames (ORFs) differed across the five ILTV genomes. Overall, 54 single nucleotide polymorphisms (SNPs) and 27 amino acid differences in 19 ORFs and 2 insertions of 9 and 3 nucleotides in two different ILTV genes were identified. Similarit ...
DNA Nanotweezers Studied with a Coarse
DNA Nanotweezers Studied with a Coarse

... Importantly, transition widths also coincide to within approximately 2 K, and thus the agreement in Tm will hold over a range of concentrations. The third basic transition is hairpin formation, in which self-complementary strands bind to themselves to form a stem and hairpin loop. Our model underest ...
ppt
ppt

... Promoter Analysis: Status of motif discovery tools • Extant tools perform reasonably well for: – Finding known/novel motifs in organisms with short, simple promoters, e.g., yeast – Identifying some of the known motifs in complex species, e.g., TFs whose BSs are usually close to the TSS • … but ofte ...
cancer genetics solutions
cancer genetics solutions

... IN CANCER GENETICS Cancer is a formidable foe that presents significant challenges. The complexity of this disease can be daunting due to the number of mechanisms that can trigger carcinogenesis, including the influence of environmental factors. However, each new discovery can reveal new understandi ...
Bioreg2017_Replication3_V4
Bioreg2017_Replication3_V4

BioInformatics Tools ppt
BioInformatics Tools ppt

... sequences in multiple alignments of gene regulatory regions • A conserved character in DNA is one that was probably present in the common ancestral species and has been preserved in the contemporary species being examined. – Two of the methods are already in common use; they are based on good column ...
Nucleic acids and chromosomes
Nucleic acids and chromosomes

... then, using GTP and peptidyl transferase causes the release of the polypeptide chain. Binding of RF causes the peptidyl transferase to transfer the peptidyl group to water rather than to an aminoacyl group. This results in the release of the polypeptide Describe the role of amino-acyl tRNAs in ensur ...
BISC 6274 - GWU Biology Department
BISC 6274 - GWU Biology Department

... 1. Have an extensive knowledge of operon systems in bacteria with selected examples; and understand molecular techniques for studying gene control in bacteria. 2. Be able to use the literature to survey the role of operons and regulons in the control of bacterial metabolism, virulence, osmoregulatio ...
Methods, Applications and Policy for Agriculture OVERVIEW
Methods, Applications and Policy for Agriculture OVERVIEW

... • Zinc finger nucleases (ZFNs) were the first widely used gene editing tools. • ZFNs were made up of two separate zinc fingers (designed to bind specifically to two separate, but closely spaced, DNA sequences) with each ZF carrying a nonspecific nuclease domain that was designed to dimerize and cut ...
8.2 Structure of DNA
8.2 Structure of DNA

... more structural genes that code for all the proteins needed to do a job. – Operons are most common in prokaryotes. – The lac operon was one of the first examples of gene regulation to be discovered. – The lac operon has three genes that code for enzymes that break down lactose. ...
Exam III
Exam III

... B) RNA acts as an expendable copy of the genetic material, allowing the DNA to serve as a permanent, pristine repository of the genetic material. C) many mRNA molecules can be transcribed from a single gene, increasing the potential rate of gene expression. D) B and C only E) A, B, and C Question 24 ...
Reaction dynamics simulation of single and double strand breaks in
Reaction dynamics simulation of single and double strand breaks in

... the probability of a two-hit event reduces much faster than a one-hit event as the scavenger concentration is increased. They also concluded that thermal effects induce SSBs but do not induce DSBs. The detailed mechanisms of strand break however remain unclear at a molecular level. We have performed ...
A kinetic proofreading mechanism for disentanglement of
A kinetic proofreading mechanism for disentanglement of

... Cells must remove all entanglements between their replicated chromosomal DNAs to segregate them during cell division. Entanglement removal is done by ATP-driven enzymes that pass DNA strands through one another, called type II topoisomerases. In vitro, some type II topoisomerases can reduce entangle ...
Detection of unpaired DNA at meiosis results in RNA‐mediated
Detection of unpaired DNA at meiosis results in RNA‐mediated

... arrested at meiotic prophase. This phenotype allowed mapping to linkage group I and cloning of sad-1þ by complementation.(2) It also has provided a more efficient method for the isolation of further Sad-1 mutations. A complete sad-1 deletion yielded almost 100% dominance while a series of sad-1 alle ...
Restriction Enzymes
Restriction Enzymes

... Restriction Enzymes: Molecular Scissors • Restriction enzymes (endonuleases) cut DNA at specific sequences • What kinds of bonds are broken when restriction enzymes cut? – Covalent bonds (within a single strand) – Hydrogen bonds (between Hydrogen strands) as a result of the bond Covalent bond stran ...
Structure of Nucleic Acids
Structure of Nucleic Acids

... Guanine and Cytosine, but Thymine appears only in DNA, whereas Uracil in RNA. (Don’t need to memorize them!) If the structure contains just the carbohydrate and the nitrogen base, it is known as a nucleoside. The names of nucleosides are given according to the type of N-base. If the nucleoside conta ...
MICR 130 Chapter 8
MICR 130 Chapter 8

... §  Study of what genes are, how they determine the characteristics of an organism, how they carry information, how the information is copied, how information is passed on to subsequent generations and between organisms §  Genome – all the genetic information in a cell §  Includes chromosomes and ...
protein - WSU Vancouver
protein - WSU Vancouver

... Primate Behavioral Ecology Genetics, Protein Synthesis, and Natural Selection ...
heredity (b)
heredity (b)

... DNA replication is crucial to the advancement of a cell in the cell cycle. In what stage does a cells chromosomal DNA go through replication? S - phase What is the principal enzyme that is responsible for DNA replication? DNA Polymerase (III) DNA replication is directional. In what direction does it ...
video slide
video slide

... expression by blocking the binding of activators to their control elements or to components of the transcription machinery or by turning off transcription even in the presence of activators. ...
Review for Heredity Unit
Review for Heredity Unit

... 3. An allele that seems to disappear (or is covered up ) when a dominant allele is present is called __________________________ 4. The passing of traits from parent to offspring. ______________________ 5. An organism’s physical appearance, or what it looks like is called its ______________________ 6 ...
Track the full extent of structural variation in a genome
Track the full extent of structural variation in a genome

... genome. Single Molecule, Real-Time (SMRT®) Sequencing provides contiguous long reads, high consensus accuracy, and uniform coverage opening up access to a broad range of structural variants (SV) across a wide range of sizes, types, and GC content. -- Discover both common and rare SVs unique to an in ...
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Helitron (biology)

A helitron is a transposon found in eukaryotes that is thought to replicate by a so-called ""rolling-circle"" mechanism. This category of transposons was discovered by Vladimir Kapitonov and Jerzy Jurka in 2001. The rolling-circle process begins with a break being made at the terminus of a single strand of the helitron DNA. Transposase then sits at this break and at another break where the helitron targets as a migration site. The strand is then displaced from its original location at the site of the break and attached to the target break, forming a circlular heteroduplex. This heteroduplex is then resolved into a flat piece of DNA via replication. During the rolling-circle process, DNA can be replicated beyond the initial helitron sequence, resulting in the flanking regions of DNA being ""captured"" by the helitron as it moves to a new location.
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