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LAB
LAB

... 6. Look again at your four plates. Do you observe some E. coli growing on the LB plates, which do not contain ampicillin/arabinose? 7. From your results, can you tell if these bacteria are ampicillin resistant by looking at them on the LB plate? Explain your answer. 8. How would you change the bacte ...
5A. Properties of Macromolecules Reading_Honors
5A. Properties of Macromolecules Reading_Honors

... last lab examining a variety of different physical and chemical properties of aqueous (waterbased) solutions. But the bodies of living things are obviously much more than water. In this lab, we will explore properties of the special carbon-based molecules that make life so complex and diverse. Carbo ...
Horizontal gene transfer and the origin of species: lessons from
Horizontal gene transfer and the origin of species: lessons from

... plasmid vehicles are rendered inactive by deletion or insertional inactivation, so that the incorporated genes are fixed. On the evolutionary scale, the acquisition of pathogenicity islands occurs at a much slower pace than that of plasmids, and would obviously create new bacterial subspecies (i.e. ...
Appendix M Questions and Guidance
Appendix M Questions and Guidance

... a-(4). How many copies are present per cell? How stable is the added DNA both in terms of its continued presence and its structural stability? ...
DNA Repair and Recombination
DNA Repair and Recombination

... • Homologous recombination plays a major role in double-strand break repair in prokaryotes and single-cell eukaryotes. • In mammalian cells, double-strand breaks are primarily repaired through NHEJ. • In mammalian cells, the main function of homologous recombination is to repair doublestrand breaks ...
DNA Repair Pathways
DNA Repair Pathways

... • Homologous recombination plays a major role in double-strand break repair in prokaryotes and single-cell eukaryotes. • In mammalian cells, double-strand breaks are primarily repaired through NHEJ. • In mammalian cells, the main function of homologous recombination is to repair doublestrand breaks ...
Blueprint of Life - The Bored of Studies Community
Blueprint of Life - The Bored of Studies Community

... Because fossils can be aged, the sequence from the very earliest life to the present can be observed, this is called the fossil record, which show a clear change from simple to very complex organisms we see today, which suggests a change over time, which is evidence of evolution, as the disparity in ...
DNA Isolation - Flinn Scientific
DNA Isolation - Flinn Scientific

... The collection of cheek cells from the inside of the mouth highlights the nature of body tissue. Dead cells are continually being sloughed off on both the inside and outside of the body. Recently-sloughed cells still contain their nuclei and their DNA genetic material. This DNA can be collected and ...
Comprehension Questions Key
Comprehension Questions Key

... radioactive dNTPS that end a DNA sequence 3. Describe the process of automated DNA sequencing. COI DNA is put in two test tubes (one with forward primers and one with reverse primers), PCR process is completed with addition of fluorescent nucleotides, sample is run on a gel to separate fragments by ...
Bacterial DNA Insert
Bacterial DNA Insert

... you want, because the construct you want is only a fraction of possible ligation products. ...
the nucleic acids
the nucleic acids

... In their experiments, they labeled the nucleotides of the old strands with a heavy isotope of nitrogen (15N) while any new nucleotides would be indicated by a lighter isotope (14N). Replicated strands could be separated by density in a centrifuge. Each model: the semi-conservative model, the conserv ...
Chapter 3 Proteins: - California State University San Marcos
Chapter 3 Proteins: - California State University San Marcos

... detected using indicator dyes ► Indirect estimates of mutation rate: comparisons of aa sequence of same protein across species ...
Lecture 21 : Introduction to Neutral Theory
Lecture 21 : Introduction to Neutral Theory

... models for evolution  Inferences about effective population size  Detection of population structure  Signatures of selection (coming attraction)  Reconstructing history of populations ...
Genetics and Heredity
Genetics and Heredity

... populations; these behaviors are directed by underlying genetic information, and responses to information are vital to natural selection and evolution. Animals have evolved sensory organs that detect and process external information. Nervous systems interface with these sensory and internal body sys ...
Structure of the DNA-binding motifs of activators
Structure of the DNA-binding motifs of activators

... • General transcription factors must interact to form the preinitiation complex • Activators and general transcription factors also interact • Activators usually interact with one another in activating a gene – Individual factors interact to form a protein dimer facilitating binding to a single DNA ...
PHYSIO | Cell Review Worksheet | Chapter 3 | KEY
PHYSIO | Cell Review Worksheet | Chapter 3 | KEY

... 39. What are the long, spaghetti‐like strands of DNA called? Chromatin  40. What do you call the pinched in portion of two sister chromatids? Centromere  41. What is special about the genetic makeup of two daughter cells? They are identical  42. Describe cytokinesis: It is the division of the cell’s ...
DNA History & Structure
DNA History & Structure

... 2. What was different about the S bacteria and the R bacteria? 3. Why were the heat-killed S bacteria harmless? 4. Why was the mixture of heat-killed S bacteria and R bacteria virulent? 5. What did Griffith discover as a result of his experiments? 6. How did Avery discover that the material responsi ...
synopsis of recombinant dna guidelines and
synopsis of recombinant dna guidelines and

... BSL 2 biosafety procedures are required for work involving viral recombinant DNA vectors. Included in this category are experiments using animal viruses as vectors, experiments in which parts of animal or plant viruses are cloned into any procaryotic or lower eucaryotic host other than E. coli or Sa ...
11. Conceptual Change and Conceptual Diversity Contribute to
11. Conceptual Change and Conceptual Diversity Contribute to

... gene applies only to sequences that have a structure something like that described above and that act as the template for making a gene product. But in humans, for example, only 1  per cent to 2  per cent of the DNA consists of sequences used to make proteins. Some—currently controversial—proportion ...
Notes S1 Simple sequence repeats and transposable elements
Notes S1 Simple sequence repeats and transposable elements

... of negative selection towards those SSRs (e.g dinucleotides) that are more likely to disrupt the functions played by these regions. We identified a dominance of tetranucleotides over trinucleotides in intronic non-coding regions. This finding is surprising since this dominance in introns is reported ...
Acute diarrhea
Acute diarrhea

... thymine occurs only in DNA and uracil is found only in RNA. RNA is present in the cytoplasm and in particularly high concentrations in the nucleolus of the nucleus. DNA is found mainly in the chromosomes. The DNA molecule is composed of two chains of nucleotides arranged in a double helix. The backb ...
Three Dimensional Organization of Genome Might Have Guided the
Three Dimensional Organization of Genome Might Have Guided the

... In eukaryotes, genes are nonrandomly organized into short gene-dense regions or “gene-clusters” interspersed by long gene-poor regions. How these gene-clusters have evolved is not entirely clear. Gene duplication may not account for all the gene-clusters since the genes in most of the clusters do no ...
Chromosome challenge activity pack
Chromosome challenge activity pack

... this can cause illness. The DNA in your cells is squashed into packages called chromosomes. Scientists study DNA and chromosomes to learn about human health. They often compare DNA from a person who has a disease with a person who doesn’t to see if there are any differences linked to the illness. Th ...
Document
Document

... S1.An RFLP marker is located 1 million bp away from a gene of interest. Your goal is to start at this RFLP marker and walk to this gene. The average insert size in the library is 55,000 bp and the average overlap at each end is 5,000 bp. Approximately how many steps will it take to get there? Answer ...
DNA PPT - Lyndhurst School District
DNA PPT - Lyndhurst School District

... Lesson Guide Lesson 1: Introduction, how do we grow? Exploring mitosis, growth, cancer and stem cells. Lesson 2: What is found in a cell. What is the nucleus? Lesson 3: What is the Structure of DNA? Lesson 4: “DNA Structure” Activity. Lesson 5: DNA Replication. Lesson 6: How are proteins made? How ...
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Helitron (biology)

A helitron is a transposon found in eukaryotes that is thought to replicate by a so-called ""rolling-circle"" mechanism. This category of transposons was discovered by Vladimir Kapitonov and Jerzy Jurka in 2001. The rolling-circle process begins with a break being made at the terminus of a single strand of the helitron DNA. Transposase then sits at this break and at another break where the helitron targets as a migration site. The strand is then displaced from its original location at the site of the break and attached to the target break, forming a circlular heteroduplex. This heteroduplex is then resolved into a flat piece of DNA via replication. During the rolling-circle process, DNA can be replicated beyond the initial helitron sequence, resulting in the flanking regions of DNA being ""captured"" by the helitron as it moves to a new location.
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