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Investigation 1: Identify the Transcriptional Unit
Investigation 1: Identify the Transcriptional Unit

... 2. To navigate to the genomic region surrounding the tra gene, enter "contig1:9,65011,000" into the "enter position or search terms" field located just above the displayed tracks and then click on the “go” button. As you learned in the previous module, you can also use the buttons in the navigation ...
Document
Document

... • DNA is synthesized from its 5’ -> 3’ end (from the 3’ -> 5’ direction of the template) • the leading strand is synthesized continuously in the 5’ -> 3’ direction toward the replication fork • the lagging strand is synthesized semidiscontinuously as a series of Okazaki fragments, also in the 5’ -> ...
DNA Shape Dominates Sequence Affinity in Nucleosome Formation
DNA Shape Dominates Sequence Affinity in Nucleosome Formation

... primarily through protein-bound complexes called nucleosomes. Such complexes consist of approximately 147 base pairs (bps) of DNA encircling a disklike protein (the histone core). Negatively charged DNA experiences a strong electrostatic attraction to the positively charged histone surface. Protein- ...
Protein Synthesis Powerpoint
Protein Synthesis Powerpoint

... Transcription : Elongation • The RNA polymerase uses only one of the strands of DNA as a template for mRNA synthesis. This is called the template strand or sense strand. The coding strand or anti-sense strand contains the complementary nucleotide sequence to the sense strand. • RNA polymerases can ...
Population Genetics - cK-12
Population Genetics - cK-12

... Darwin knew that heritable variations are needed for evolution to occur. However, he knew nothing about Mendel’s laws of genetics. Mendel’s laws were rediscovered in the early 1900s. Only then could scientists fully understand the process of evolution. We now know that variations of traits are herit ...
Exploring Gene Therapy.indd
Exploring Gene Therapy.indd

... functional copy. It is important that they understand this is not the case. Gene therapy endeavors to add a functional copy of the gene of interest in the hope that its function will counteract the effect of the faulty gene. ...
Sequence logos for DNA sequence alignments
Sequence logos for DNA sequence alignments

... to 2 bits. A position in the motif at which all nucleotides occur with equal probability has an information content of 0 bits, while a position at which only a single nucleotide can occur has an information content of 2 bits. The information content at a given position can therefore be thought of as ...
Chapter 17 Nucleic Acids and Protein Synthesis Nucleic Acids
Chapter 17 Nucleic Acids and Protein Synthesis Nucleic Acids

... 17.1 Components of Nucleic Acids 17.2 Primary Structure of Nucleic Acids ...
Alkaline Phosphatase - Lake Forest College
Alkaline Phosphatase - Lake Forest College

... Figure 4. A ClustalW alignment of the S. griseus StrK gene and Human ALPL gene was performed. The ClustalW alignment of StrK, the gene responsible for alkaline phosphatase in S. griseus and Human alkaline phosphatase ALPL gene revealed high conservation of amino acids between the two species. This s ...
Topic 10: « MODERN METHODS OF DNA DIAGNOSIS OF
Topic 10: « MODERN METHODS OF DNA DIAGNOSIS OF

Cell Reproduction - Rocky Mountain Middle School
Cell Reproduction - Rocky Mountain Middle School

...  1) Protein synthesis is the process of decoding DNA ...
Race for the Double Helix discussion questions
Race for the Double Helix discussion questions

... regularity of a crystal, Watson is convinced that DNA might be analyzed by straightforward methods that have previously succeeded in solving the structure of other types of crystals. This conviction carries Watson to England, where the technique of X-ray crystallography is most advanced. However, si ...
Review Process - The EMBO Journal
Review Process - The EMBO Journal

... mutations that were not explained by the Apo structure because of DNA-binding-induced conformational changes. There are indeed many more clinically discovered mutations but most have not yet been characterized biochemically. In collaboration with our clinical colleagues in Houston, we are in the pro ...
Demonstration that the Neurospora crassa mutation un
Demonstration that the Neurospora crassa mutation un

... assembly 7 of the Neurospora genome (Galagan et al., 2003), this cosmid maps to linkage group IV on contig 43, while un-4 and lys-5 are on linkage group VI. Based on the location of lys-5 (NCU05526) on contig 22, we chose several cosmids from contig 22 and found that two did restore the ability to g ...
Visualizing MD Results: Mechanical Properties of dsDNA Mini Tutorial
Visualizing MD Results: Mechanical Properties of dsDNA Mini Tutorial

... The system we have loaded was used to investigate the microscopic mechanics of the DNA when it is stretched. In this section, we take a closer look at how the simulation system was designed. 1. Change the first representation (segname ADNA BDNA and backbone) Drawing Method to Licorice. There are two ...
Protocol DNA Isolation from Bacteria by nexttec 1
Protocol DNA Isolation from Bacteria by nexttec 1

... nexttecTM 1-Step DNA Isolation Kit components were developed, designed and sold for research purposes only. They are suitable for in vitro uses only. No claim or representation is intended for use to identify any specific organism or for clinical use. It is the responsibility of the user to verify t ...
Promoter-trapping in Saccharomyces cerevisiae
Promoter-trapping in Saccharomyces cerevisiae

... 2001, the site of insertion has been characterised in more than 22 000 insertion clones, less than two-thirds of the about 6200 yeast genes are represented in this collection (7). In addition to gene-size dependent biases in targeting ef®ciency, nonrandom insertion of Tn3-derived transposons (8) and ...
Extranuclear Inheritance
Extranuclear Inheritance

... Mutations in chloroplasts and mitochondria have been useful in identifying organelle inheritance patterns. Inheritance of these mutations was determined by the makeup of the organelle DNA. It is important to note that an individual cell has hundreds of chloroplasts and/or mitochondria, which may not ...
module 2: transcription part i
module 2: transcription part i

... 2. To navigate to the genomic region surrounding the tra gene, enter "contig1:9,65011,000" into the "enter position or search terms" field located just above the displayed tracks and then click on the “go” button. As you learned in the previous module, you can also use the buttons in the navigation ...
CH 8. DNA: The Universal Molecule of Life
CH 8. DNA: The Universal Molecule of Life

... The lac operon consists of an operator (the binding site for the repressor protein), a promoter (binding site for RNA polymerase) and three genes that code for enzymes involved in lactose breakdown. A repressor protein regulates the three genes by binding to the operator and inhibiting transcription ...
Sweaty T-Shirts and Human Mate Choice Type the following URL
Sweaty T-Shirts and Human Mate Choice Type the following URL

... 4. What is the benefit of comparing the DNA of different species? We can establish how closely related they are. 5. What is a mutation? A mistake in copying DNA sequences. 6. Why must you use the same sequences in the same gene if you’re comparing two species? Otherwise it’s an invalid comparison. 7 ...
Directed Evolution with Fast and Efficient Selection Technologies
Directed Evolution with Fast and Efficient Selection Technologies

... Fig. 1. Mutagenesis strategies for localized and whole-gene mutagenesis. Error prone PCR is depicted schematically in panel (A) as a strategy for the undirected distribution of mutations over a whole gene of interest. Two types of mutations are shown, favourable ones (open squares) and unfavourable ...


... The two genes have splice variants that are targeted to different compartments Samach et al 2011 Plant Cell 23:4266 ...
DNA profiling : standardising the report
DNA profiling : standardising the report

... and the human DNA fragments behave in the same way during electrophoresis. However, it is known that DNA molecules of the same size do not necessarily move at the same rate under electrophoresis (Elder & Southern 1983; Lalande et al. 1988) this being strikingly apparent in dealing with the problem o ...


... The two genes have splice variants that are targeted to different compartments Samach et al 2011 Plant Cell 23:4266 ...
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Helitron (biology)

A helitron is a transposon found in eukaryotes that is thought to replicate by a so-called ""rolling-circle"" mechanism. This category of transposons was discovered by Vladimir Kapitonov and Jerzy Jurka in 2001. The rolling-circle process begins with a break being made at the terminus of a single strand of the helitron DNA. Transposase then sits at this break and at another break where the helitron targets as a migration site. The strand is then displaced from its original location at the site of the break and attached to the target break, forming a circlular heteroduplex. This heteroduplex is then resolved into a flat piece of DNA via replication. During the rolling-circle process, DNA can be replicated beyond the initial helitron sequence, resulting in the flanking regions of DNA being ""captured"" by the helitron as it moves to a new location.
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