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Package `GESTr`
Package `GESTr`

... The TranSAM algorithm constructs balanced permutations of the input data and uses these to estimate the false-discovery rates of identifying genes as belonging to different expression states in the two specified sample groups. The balanced permutations are constructed so that an equal number of samp ...
19 DetailLectOut 2012
19 DetailLectOut 2012

... Each type of virus can infect and parasitize only a limited range of host cells, called its host range. ○ This host specificity depends on the evolution of recognition systems by the virus. ○ Viruses recognize host cells by a “lock and key” fit between proteins on the outside of the virus and specif ...
genomebiology.com
genomebiology.com

... to the family of CR1 repeats) account for 79% of all TEs. In the D. rerio genome, more than 75% of TEs are DNA transposons; whereas in D. melanogaster, LTRs are the most abundant TEs, accounting for 44% of the elements observed. Finally, DNA transposons account for 95% of TEs in C. elegans. These di ...
Practical class № 1 (1)
Practical class № 1 (1)

... 2. Existence of life on all levels is determined the structure of lower level. What level of organization existence of life is preceded and provides on cellular level: A. Molecular B. Tissue C. Organismic D. Population-species E. Biocenotic 3. To the light part of microscope belong: A. Mirror B. Rev ...
Chapter 18: Altering the Genetic Message
Chapter 18: Altering the Genetic Message

... from one location to another in the genome, using an enzyme to cut and paste themselves into new genetic neighborhoods. We call these mobile bits of DNA transposable elements, or transposons. Transposons select their new locations at random, and are as likely to enter one segment of a chromosome as ...
Presentation
Presentation

... • Initial stages of analyzing DNA sequence: – Find genes – Find and Analyze similar genes – Multialign like genes to find active sites ...
Review for Final
Review for Final

... 74) What are treatments to prevent viral infection (more than 1)? Chap 19 75) Briefly describe the role of the different histone proteins in chromatin packaging. 76) Briefly describe the difference between heterochromatin and euchromatin. 77) What is differential gene expression? 78) Define the func ...
DNA Profile 911 - Bertino Forensics
DNA Profile 911 - Bertino Forensics

... When trying to identify someone from their DNA, their physical appearance is not being examined. Instead, personal identification is based on their genes or alleles for different STRs. The more STRs being examined, the more reliable is the identification of the person. Today, DNA is analyzed using t ...
Functional Analysis of A Novel Splicing Mutation in The Mutase
Functional Analysis of A Novel Splicing Mutation in The Mutase

... sequence consists of two distinct functional domains: a substrate-binding site (residues 88-422), and a C-terminal vitamin B12-binding domain (residues 578-750). The C-terminal domain contains the enzyme active site (3, 6-8). Molecular analysis of the genes involved in inherited metabolic diseases h ...
Molecular fingerprinting of Cryptosporidium
Molecular fingerprinting of Cryptosporidium

... basing results on analysis at 1 locus can be misleading and confirmation from different loci provides greater strength to the analysis. Here, one locus (N18SDIAG) performed consistently better than the other 3 tested. In our initial studies on PCR assay sensitivity with C. parvum oocysts, N18SDIAG w ...
human genetic disorders part 2–diagnosis and treatment
human genetic disorders part 2–diagnosis and treatment

... A modification of previously described CAPS method is Derived Cleaved Amplified Polymorphic Sequences (dCAPS) method where PCR primer possesses a mismatched nucleotides in order to create a restriction site if a specific nucleotide change occurs in the target sequence. Genotyping based on PCR-RFLP ...
Advanced Computational Structural Genomics
Advanced Computational Structural Genomics

... biology: the 3D structures of proteins have been better conserved during evolution than their genome sequences. When the similarity of a target sequence to another sequence with known structure is above a certain threshold, comparative modeling methods can often provide quantitatively accurate prote ...
Codon optimization
Codon optimization

Myotonic dystrophy DM
Myotonic dystrophy DM

... -Most of the expansion disorders exhibit a delayed onset form of their diseases, indicating that they may share mechanisms that postpone clinical expression until later in life. -Depending on where the unstable repeats are located within the gene, the repeat expansion disorders can be classified as ...
TUTORIAL 8 – DNA - Molecular Movies
TUTORIAL 8 – DNA - Molecular Movies

... roughly based on what is known about the molecule’s proportions, and then look at different ways to deform it. Next we’ll import a PDB coordinate set for B-DNA and experiment with different representations using particles. These first two methods assume that the helix does not need to unwind and mel ...
14 Phylogenomic Approach to the Evolutionary Dynamics of Gene
14 Phylogenomic Approach to the Evolutionary Dynamics of Gene

... 2004). Birds may also have fewer protein-coding genes in their genomes than mammals have, with roughly 18,000 in chickens compared with approximately 22,000 in humans (Hillier et al., 2004). By comparing the genomes of chicken with those of humans and pufferfish (Fugu), the difference in gene count ...
013368718X_CH13_193
013368718X_CH13_193

... The sugar in RNA is ribose instead of deoxyribose. RNA is generally single-stranded and not double-stranded like DNA. RNA contains uracil in place of thymine. RNA can be thought of as a disposable copy of a segment of DNA. Most RNA molecules are involved in protein synthesis. The three main types of ...
6_Petrin_prot_DBs_2011
6_Petrin_prot_DBs_2011

... Databases describing functions • Gene Ontology databases – Classify genes into categories that describe gene function – Standardized classification applicable to all species – Classes represent involvement in biological tasks (like protein synthesis), chemical activities (like carbohydrate binding) ...
EXTRACTION OF GENE-DISEASE RELATIONS FROM
EXTRACTION OF GENE-DISEASE RELATIONS FROM

... such as M edLine have a vast amount of knowledge. Our aim in this paper is to extract diseases and their relevant genes from M edLine abstracts, which we term relation extraction. There are some existing systems for relation extraction from biomedical literature. ArrowSmith (Swanson 1986) 1 and BITO ...
Genetic Technology - McGraw Hill Higher Education
Genetic Technology - McGraw Hill Higher Education

... manufacture short pieces of DNA of any sequence it is programmed to produce. The DNA synthesizer cannot easily make entire genes, but it can make small fragments that can act as primers to DNA replication. If one primer is made for each end of the region of interest, they act to bracket the region t ...
Co-dominant SCAR marker, P6-25 - Department of Plant Pathology
Co-dominant SCAR marker, P6-25 - Department of Plant Pathology

... 1 min. These cycles were followed by 72 C for 10 min, and then the reaction was held at 4 C. PCR reactions were performed in the MJ DNA Engine PT200 Thermocycler™ (MJ Research Inc., Waltham, MA). PCR-amplified fragments were separated by gel electrophoresis with 1.5% agarose in 0.5 X TBE buffer, sta ...
PHYCOCYANIN ALPHA AND BETA SUBUNITS OF Anabaena
PHYCOCYANIN ALPHA AND BETA SUBUNITS OF Anabaena

... conversion of heme to phycocyanobilin (PCB), and cpcA encoding alpha subunit along with ...
B  : S -
B : S -

... Biodiversity assessments typically consist of identifying species and evaluating one or more metrics: (1) species richness (number of species), (2) evenness (ratio of various species), and/or (3) character diversity (phenotypic difference) (Purvis and Hector, 2000). Because it is the simplest way to ...
an introduction to DNA barcoding
an introduction to DNA barcoding

... the often tedious and rather naı̈ve polemics that have surrounded the barcoding initiative. Instead, the main scientific issues debated were (i) is it possible to distinguish a large number of species using short DNA sequence data? (ii) can closely related or fastevolving species be distinguished wi ...
Patients - HAL
Patients - HAL

... Among the 13 sequence changes that we are reporting, the c.730_731delATinsG presumably severily impairs the peropsin function. This deletion/insertion will cause the truncation of the 94 C-terminal amino acids, that is 27.9 % of the protein. Thus, the alteration deletes the last 2 transmembrane span ...
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Helitron (biology)

A helitron is a transposon found in eukaryotes that is thought to replicate by a so-called ""rolling-circle"" mechanism. This category of transposons was discovered by Vladimir Kapitonov and Jerzy Jurka in 2001. The rolling-circle process begins with a break being made at the terminus of a single strand of the helitron DNA. Transposase then sits at this break and at another break where the helitron targets as a migration site. The strand is then displaced from its original location at the site of the break and attached to the target break, forming a circlular heteroduplex. This heteroduplex is then resolved into a flat piece of DNA via replication. During the rolling-circle process, DNA can be replicated beyond the initial helitron sequence, resulting in the flanking regions of DNA being ""captured"" by the helitron as it moves to a new location.
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