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Molecular Biology
Molecular Biology

... 7. Check purity (A260/A280 = 2) 8. Check the integrity of rRNA by gel electrophoresis (denaturing agarose gel) ...
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... formed. In plant cells, the cell plate, formed by cellulose, breaks the cell in half physically. In animal cells, the cleavage furrow pinches the cell into two cells.  Cytokinesis—divides the entire cell into two pieces after telophase. o CDK, or cyclin dependent kinase, adds ATP to other substance ...
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... GM crops are the most extensively tested crops ever added to the food supply. • GM plants must be shown to shown to be the same as the parent crop from which it was derived • If a new protein trait has been added, the protein must be neither toxic nor allergenic ...
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... are short polymers consisting of nucleic acid analogs. Morpholino antisense oligomers are introduced into tissues of viable embryos where they bind to any respective complementary RNA sequence and block access of that transcript by translational machinery. If a cell type is lost or functioning aberr ...
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... (d). What would be the expected phenotypic ratio from a cross of RR’dd plants with R’R’Dd plants? ...
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... Supplementary Table S1. The table contains the oligonucleotide sequence of all the primers used for RT-PCR analysis as described in the main text. The primers were designed using online Primer3 software. (FW-Forward primer, RV- Reverse primer). ...
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... AtEm1 locus (GenBank accession no. AF049236). We then searched for possible deletion by PCR in the 15 predicted ORFs (open reading frame) in this region, and found a 50 base-pair deletion affecting the second ORF (At3g51770) in two X-ray generated alleles, eto1-2 and eto1-3. Further sequencing and P ...
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... in the two classes, based on their expression profiles • Once learning is done, the SVM may be presented with previously unseen genes (“test data”) • Should be able to recognize the genes as members or non-members of the functional class ...
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... Practical limit = 1015 = ~ 2 nmoles = ~ 50 ug DNA 1015 is a large number. Very large (e.g., 500,000 times as many as all the unique 40-mers in the human genome.) These 1015 sequences are known as “sequence space” Each DNA molecule of these 1015 (or RNA molecule copied from them) can fold into a part ...
Support Vector Machines and Gene Function Prediction
Support Vector Machines and Gene Function Prediction

... in the two classes, based on their expression profiles • Once learning is done, the SVM may be presented with previously unseen genes (“test data”) • Should be able to recognize the genes as members or non-members of the functional class ...
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... Practical limit = 1015 = ~ 2 nmoles = ~ 50 ug DNA 1015 is a large number. Very large (e.g., 500,000 times as many as all the unique 40-mers in the human genome.) These 1015 sequences are known as “sequence space” Each DNA molecule of these 1015 (or RNA molecule copied from them) can fold into a part ...
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... • Each trait is determined by a pair of genes – one from each parent. • Both parents contribute equally to your genes. • A gene is either dominant or recessive. • Three possible gene pairs for any trait: ...
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... the second in frame methionine (codon 43) and from the first in frame methionine (codon 1); it has been demonstrated that these two isoforms are phosphorylated; these proteins belong to the ETS transcription factors family characterized by the presence of 85 amino acids, the ETS domain; this domain ...
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... microRNA target prediction 1.2 Is there a complementary sequence within the 3'UTR of PC to postion 2-8 in the sequence of microRNA hsa-mir-182. UCSC genome browser 1.3 Position of transcript start site and transcription end of Pyruvate carboxylase (NM_000920) in hg19 assembly ...
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... • Was the predictive value of these SNPs still seen in multivariate analysis that included known clinical prognostic variables such as tumor grade, baseline CA19-9, + or - margin or LN? ...
Identify differential APA usage from RNA-seq
Identify differential APA usage from RNA-seq

... we perform the alignment separately for genes on the plus and minus strand, thus the reads that overlap genes on different strands will be counted for all of them, while those on genes on the same strand will be correctly discarded. In order to overcome this problem in the multipleAPA analyses it is ...
Screening of Gene Markers for Forensic Identification of Vaginal
Screening of Gene Markers for Forensic Identification of Vaginal

... particular exon that might not be expressing but another exon of the same gene may be expressing so it is recommended to make multiple primers for a single gene to cover all the reported exons of a single gene. This study was a preliminary investigation in order to gain an understanding of the usage ...
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RNA-Seq



RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.
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