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Integrative Genome-wide Analysis of the Determinants of RNA
Integrative Genome-wide Analysis of the Determinants of RNA

... demonstrates that we do not only find sQTL for tumor specific splicing events 1 B but are also able to identify functionally annotated variants providing potential new mechanistic insights. Our analysis revealed a subset of genes that showed large differences in splicing events between tumor and nor ...
Zeeberg - Gene Ontology Consortium
Zeeberg - Gene Ontology Consortium

... • GoMiner traditionally dereplicates input files so that only one instance of a gene name is processed • When multiple alternatively spliced forms are to be analyzed, however, dereplication would result in a loss of relevant information • Consequently, we have added a new feature to GoMiner to retai ...
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1- Renal function can be impaired in children with primary

... for PH3 and to a lesser extent for PH2, is likely due to underdiagnosis of these diseases, which have overall milder phenotypes and are much less likely than PH1 to result in ESRD. In addition, relatively mild disease in a subset of PH1 patients (especially p.G170R homozygotes) and a wide variabilit ...
Von Neumann`s Quintessential Message: Genotype C Ribotype D
Von Neumann`s Quintessential Message: Genotype C Ribotype D

... In 1958, one year after John von Neumann’s death, two major events took place in the history of molecular biology: First, Francis Crick, one of the discoverers of the DNA double helix, put forward what he called the central dogma of molecular biology: Proteins are not made directly from genes—there ...
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... 15. What are the names of two signaling pathways that are involved in the specification of the Spemann-Mangold Organizer (p. 312)? Wnt, as well as Nodal and BMP (bothTGF-beta signaling pathways) signaling events are involved in the specification of the Spemann-Mangold Organizer. ...
MGY428- Genomes
MGY428- Genomes

... heterochromatin assembly that replaces sequence specific binding sites Telomeres are found at the end of chromosomes and are composed of simple tandem repeats which protect the integrity of the ends They are dynamic – for many cell types during every round of replication, they shrink. This limits th ...
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... Point mutations can result from replication and proofreading errors, or from environmental mutagens. Silent mutations have no effect on the protein because of the redundancy of the genetic code. Silent mutations result in genetic diversity not expressed as phenotype ...
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Heredity PowerPoint

... would result in all being identical (All Dominant) • 3rd – the F2 generation would result in a 3:1 ratio (3 Dominant: 1 Recessive) ...
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Drug - CBS

... In ATC classification system, the active substances are divided into different group according to the organ or system on which they act and their therapeutic, pharmacologcal and chemical properties ...
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Genetics and Heredity

... and yellow paints blend to make green. What would happen if this was the case? ...
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... RNA polymerase II transcribes protein-encoding genes, or messenger RNAs, which are the RNAs that get translated into proteins. Also, most snRNA (splicing) and microRNAs (RNAi). This is the most studied type, and due to the high level of control required over transcription a range of transcription fa ...
alleles in gene pair are identical
alleles in gene pair are identical

... form of a trait resulting from a gene pair • Example: yellow or green seed color ...
Mendelian Genetics: Heredity
Mendelian Genetics: Heredity

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Ch. 14 The Human Genome

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Mutations Worksheet

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Real time PCR or Quantitative PCR

... conventional rape seed. ● Cancer or disease detection ○ Multiplex real-time reverse transcriptase PCR is an applicable method for the detection, identification, and quantification HBV, HCV and HIV-1 ○ Bernard and Wittwer (2002) used real-time PCR for detection of multiple breast cancer molecular mar ...
Chapter 21: Molecular Basis of Cancer
Chapter 21: Molecular Basis of Cancer

... •Ligation seals the nick, and exonuclease I is used to remove excess unannealed and unligated circular probes •The resultant product is PCR-amplified and the orientation of the primers ensures that only circularized probes will be amplified •The resultant product is hybridized and read out on an ar ...
Nucleic Acids and DNA
Nucleic Acids and DNA

... C. Nucleotides (total molecule) • Have a phosphate on carbon #5 • Can have up to 3 phosphates • Monophosphate (NMP) • Diphosphate (NDP) • Triphosphate (NTP) – Where N is any one of the nucleic acids ...
Everything you wanted to know about ENCODE
Everything you wanted to know about ENCODE

... Proteins that bind to specific DNA sequences in the promoter region together turn a gene on or off. These proteins are themselves regulated by their own promoters leading to a gene regulatory network with many of the same properties as a neural network. ...
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... Use the directions given in the tutorial to start a new search: Search for the “Human Cytochrome C” nucleotide sequence, scroll down until you reach “NCBI reference sequence” and then click on the FIRST blue hyperlink under the “mRNA and Protein” heading. Scroll down and copy the entire sequence. Pa ...
CHAPTER 2. GENE IDENTITY BY DESCENT 2.1 Kinship and
CHAPTER 2. GENE IDENTITY BY DESCENT 2.1 Kinship and

... markers one probably shouldn’t), genes that are ibd must be of the same allelic type, while genes that are not ibd are of independent allelic types. Gene identity by descent is defined only within the context of a defined pedigree. A pedigree specifies the two parents of every non-founder individual ...
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... – This creates new allele combinations that are different to those of the parent. ...
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... w/approximately 3 billion nucleotide pairs of DNA that carry between 50,000 & 100,000 genes •If genome’s chromosomes were uncoiled and laid end to end, they would make a very thin thread that would be approximately 3 meters long ...
Ch 14 & 15, Genetics, FALL 2011
Ch 14 & 15, Genetics, FALL 2011

... out by painstakingly doing crosses involving linked genes. This was done for many of the model systems used by geneticists, fruit flies, certain fungi, corn, tomatoes, etc. Aberrations from the expected results also indicated that certain DNA sequences could actually move their location over time. T ...
Candidate gene copy number analysis by PCR and multicapillary
Candidate gene copy number analysis by PCR and multicapillary

... analysis of the same samples. Concentrations of the PCR primers were adjusted so that in the case of normal copy numbers the peak areas of the two genes were approximately the same. In this way a 1.5 Nr1i2/RNase P normalized area ratio refers to an Nr1i2 copy number 3, while 0.5 refers to Nr1i2 copy ...
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RNA-Seq



RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.
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