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Somaclonal Variation
Somaclonal Variation

... Somaclonal Variation • Possible causes – the "culture environment" • the hypothesis – that tissue culture is inherently stressful to cultured plant cells • environmental stress is known to cause: – DNA methylation – the methylation of cytosine is known to cause gene inactivation; this may occur dur ...
Title - Iowa State University
Title - Iowa State University

... 1. What are the three similarities between chromosome behavior and Mendel’s factors? a) Both are present in pairs in diploid cells b) Homologous chromosomes separate and factors segregate during meiosis c) Fertilization restores the paired condition of both factors and chromosomes 2. The ___________ ...
Composite Transposons
Composite Transposons

... Bacteriophage Mu ...
Expression of the Vacuolar Iron Transporter
Expression of the Vacuolar Iron Transporter

... investigated the function of a small family of five membrane proteins that have amino acid sequence homology to AtVIT1 and consequently also to yeast CCC1p (1-3). These Vacuolar-Iron-Transporterlike (VTL) genes are found both in mono‐ and dicotyledon plants, as well as in the green alga Chamydomonas ...
Mutations Worksheet
Mutations Worksheet

... Syndrome in one family. The number of times that the trinucleotide CGG is repeated is given under the symbols. The gene is on the X chromosome, so women have two copies of it; men have only one. People with a gene containing 80–90 repeats are normal, but this gene is unstable, and the number of repe ...
Acute diarrhea
Acute diarrhea

... Nucleic acid is composed of a long polymer of individual molecules called nucleotides. Each nucleotide is composed of a nitrogenous base, a sugar molecule and a phosphate molecule. The nitrogenous bases fall into two types, purines and pyrimidin, the purines include adenine and guanine; the pyrimidi ...
Models for Structural and Numerical Alterations in Cancer
Models for Structural and Numerical Alterations in Cancer

... scenario with a minimum number of duplications is NP-hard. • Reduction from Hamiltonian path problem on a directed graph with in/out degree 2. ...
Use of Illumina sequencing to identify transposon insertions
Use of Illumina sequencing to identify transposon insertions

... oligonucleotide corresponding to the Mu terminal inverted repeat. This method yields dense clusters of sequence reads that tile approximately 400 bp flanking each side of each heritable insertion. The utility of the approach is demonstrated by identifying the causal insertions in four genes whose di ...
Ch 3 Answers to Applying and Data Questions
Ch 3 Answers to Applying and Data Questions

... and cannot bind the substrate; there is very low activity. Enzyme + RNA: RNA has no effect on enzyme activity, and the reaction can occur much faster (as with enzyme alone). Enzyme + dipeptide: The dipeptide has a –C–C–CO–N–C– structure that is similar to that of the substrate. The dipeptide inhibit ...
Molecular Strategies for detection of insertion of genes in transgenic
Molecular Strategies for detection of insertion of genes in transgenic

... • So expression of transgenes has been attributed to: – copy number - the number of transgene copies integrated into the host genome – “positional effects” - the position of the T-DNA integration site in the host genome may affect the level of expression – variable arrangements of transgene sequence ...
SexLinked
SexLinked

... All X chromosomes have locations for the genes for hemophilia, as well as color-blindness and other sex-linked traits. Therefore, we still use the system of letters, such as E and e, to represent forms of these genes as superscripts on the X chromosome. For example, the normal gene for blood clottin ...
Non-Mendelian Genetics
Non-Mendelian Genetics

... • Is there any correlation between any of the markers and the trait? ...
Books received
Books received

... plant breeder, and also Richard Jefferson's ominous discussion of the problems encountered in the detection of gene expression in the field, coupled with a spirited plea in favour of the GUS marker system. Books of this kind are required for departmental libraries, and judging from the number of tim ...
Transcription and Translation
Transcription and Translation

... • Transcription Factors = proteins that attract the RNA polymerase and regulate • RNA Polymerase = Enzyme that completes process of transcription • mRNA = messenger RNA – Copy of template strand that makes protein ...
Transcription and Translation
Transcription and Translation

... • Transcription Factors = proteins that attract the RNA polymerase and regulate • RNA Polymerase = Enzyme that completes process of transcription • mRNA = messenger RNA – Copy of template strand that makes protein ...
YYRR
YYRR

... • Is there any correlation between any of the markers and the trait? ...
Using Genetic Markers
Using Genetic Markers

... • Single nucleotide polymorphisms (SNPs) are sites in DNA where some individuals in the population have different bases. ...
article in press
article in press

... Crick, 1970) stated: ‘‘DNA makes RNA, RNA makes proteins’’. Repetitive DNA sequences, which in such a model could not contain any significant genetic information, were regarded as junk DNA with no real function. Similarly introns, as non-coding sequences present within genes, were often considered as ...
three possibile models for replication
three possibile models for replication

... During transcription, the order of nitrogenous bases on DNA determines the order of complementary bases on the mRNA molecule that is created from that DNA sequence. A group of three bases on an mRNA molecule is called a codon. (Sometimes, the group of three bases on DNA that codes for a particular c ...
word
word

... Copy each of these sequences either into an excel sheet or directly into ApE (see description in 8a where you use “Find” and highlight options. At this point go to Step 3 and Click on GBrowse (green rectangle) to get the gene sequence from the short chromosome and follow the same procedure upto Ste ...
Conclusions Synapsin IIa is expressed in the brain of adult zebrafish
Conclusions Synapsin IIa is expressed in the brain of adult zebrafish

... Figure 1: RT-PCR analysis of Synapsin IIa from zebrafish embryos. The SynIIa gene was amplified from zebrafish brain, 24 hpf, and 72 hpf. The primers used encompassed the predicted ATG start codon and stop codon to produce an amplicon of 1420 base pairs ...
Document
Document

Document
Document

... – Construct “category term vector” Vc for each category c – Weight of term ti in this vector is wij=TFij*IDFi • TFij is frequency of ti in all training sentences of category j • IDFi is “inverse document frequency” = 1+log(N/ni), N = total # documents, ni = number of documents containing ti. • TF me ...
Biology Fact Sheet
Biology Fact Sheet

... Heredity — passing on of characteristics from parents to offspring Homozygous - when there are two identical alleles for a trait (TT, tt) Heterozygous - when there are two different alleles for a trait (Tt) Hybrid — offspring formed by parents having different forms of a specific trait. (Tt) Recessi ...
Chapter 5 Gases - Annmarie Kotarba | Nurse, Teacher and
Chapter 5 Gases - Annmarie Kotarba | Nurse, Teacher and

... • Transcriptional-level control predominates, but control at other levels of gene expression is also very important, especially in multicellular organisms • In many instances, pre-formed enzymes and other proteins are rapidly converted from an inactive to an active state • In multicellular organisms ...
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RNA-Seq



RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.
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