1 - life.illinois.edu
... a. How could you select for revertants of T4 rII mutants? [Revertants could be isolated by plating a pool of rII phage on K12 (λ+); only revertants will be able to form plaques. This is a selection. Looking for wild type plaques on B would require a lot more work since most of the plaques would be r ...
... a. How could you select for revertants of T4 rII mutants? [Revertants could be isolated by plating a pool of rII phage on K12 (λ+); only revertants will be able to form plaques. This is a selection. Looking for wild type plaques on B would require a lot more work since most of the plaques would be r ...
Lecture15
... Chromosome and genome comparisons versus gene comparisons • Comparisons of genes, proteins and non-coding sequences is not the only way to study relations between different species. • Attempts were made from 1930s to use chromosome rearrangements information for this purpose. • It has been shown th ...
... Chromosome and genome comparisons versus gene comparisons • Comparisons of genes, proteins and non-coding sequences is not the only way to study relations between different species. • Attempts were made from 1930s to use chromosome rearrangements information for this purpose. • It has been shown th ...
GenomeBrowser - CBS
... UCSC genome browser Basic functionalities • Finding a gene • by name • by sequence • Gene structure • Sequence orthologues • Single Nucleotide Polymorphisms • Gene Sorter - sort according to expression, homology ... • Custom tracks ...
... UCSC genome browser Basic functionalities • Finding a gene • by name • by sequence • Gene structure • Sequence orthologues • Single Nucleotide Polymorphisms • Gene Sorter - sort according to expression, homology ... • Custom tracks ...
Identification of candidate genes for resource-use
... For the second a Kruskal-Wallis one way ANOVA is performed, relating the trait to the combinations of three consecutive markers centred on the current one. For both tests, asymptotic p values are used, calculated with the R package coin (Horthorn et al, 2008). For each chromosome, the markers in th ...
... For the second a Kruskal-Wallis one way ANOVA is performed, relating the trait to the combinations of three consecutive markers centred on the current one. For both tests, asymptotic p values are used, calculated with the R package coin (Horthorn et al, 2008). For each chromosome, the markers in th ...
PowerPoint Presentation Materials to accompany
... • screening a DNA library based on detection of protein expression from cloned gene – Antibody probes – Protein probes – DNA binding sites (transcription factors) Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings ...
... • screening a DNA library based on detection of protein expression from cloned gene – Antibody probes – Protein probes – DNA binding sites (transcription factors) Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings ...
The HapMap project and its application to genetic
... the public domain as a result of the Human Genome Project and associated research. The more ambitious approach would be to scan the entire genome for important new variants—an approach which is not limited by any prior hypothesis, but which requires effective resources and technology for genome-wide ...
... the public domain as a result of the Human Genome Project and associated research. The more ambitious approach would be to scan the entire genome for important new variants—an approach which is not limited by any prior hypothesis, but which requires effective resources and technology for genome-wide ...
Gene Ontology and Annotation
... Provides functional annotation for small model organisms (functional descriptions of genes or proteins) ...
... Provides functional annotation for small model organisms (functional descriptions of genes or proteins) ...
No Slide Title
... There are at least 100 light responsive genes (e.g. photosynthesis) There are many cis-acting, light responsive regulatory elements 7 or 8 types have been identified of which the two for CHS are examples No light regulated gene has just 1. Different elements in different combinations and c ...
... There are at least 100 light responsive genes (e.g. photosynthesis) There are many cis-acting, light responsive regulatory elements 7 or 8 types have been identified of which the two for CHS are examples No light regulated gene has just 1. Different elements in different combinations and c ...
Microbial Genetics Lecture PowerPoint
... Images: Binary Fission, JW Schmidt, Phylogenetic Tree, Eric Gaba, NASA Astrobiology institute. ...
... Images: Binary Fission, JW Schmidt, Phylogenetic Tree, Eric Gaba, NASA Astrobiology institute. ...
Life Science
... Trait: A characteristic that can take alternative forms, such as plant height. Allele: Each variation of a given gene, expressed as either an upper or lower case letter. ...
... Trait: A characteristic that can take alternative forms, such as plant height. Allele: Each variation of a given gene, expressed as either an upper or lower case letter. ...
MORGAM (an international pooling of cardiovascular cohorts)
... risk factors in different parts of Europe, between men and women, and between age groups using large cohorts with standardized baseline measurements and carefully validated fatal and nonfatal CHD and stroke end-points. Furthermore, the data will be used to derive European risk scores and to assess t ...
... risk factors in different parts of Europe, between men and women, and between age groups using large cohorts with standardized baseline measurements and carefully validated fatal and nonfatal CHD and stroke end-points. Furthermore, the data will be used to derive European risk scores and to assess t ...
Chapter 14: Mendelian Genetics Early Inheritance Ideas
... P (parent) generation crossed F1 (first filial) is offspring of P gen. F2 (second) filial is offspring of cross b/w F1 individuals F1 always 100% purple Purple males & females used to eliminate sex as a factor F2 a 3:1 ratio of purple to white o Mendel’s Conclusions Unit Characters (Pa ...
... P (parent) generation crossed F1 (first filial) is offspring of P gen. F2 (second) filial is offspring of cross b/w F1 individuals F1 always 100% purple Purple males & females used to eliminate sex as a factor F2 a 3:1 ratio of purple to white o Mendel’s Conclusions Unit Characters (Pa ...
AP Biology Deoxyribonucleic acid
... http://www.biology.arizona.edu/biochemistry/problem_sets/large_molecules/06t.html ...
... http://www.biology.arizona.edu/biochemistry/problem_sets/large_molecules/06t.html ...
PDF file
... strategy to inactivate the gene. The pKO-AMG plasmid vector contained the bacterial betagalactosidase gene which he used to create an AMG-LacZ gene as an in-frame fusion to exon 2 sequences. Following standard gene replacement methods, he generated a founder mouse strain from brown agouti ES cells a ...
... strategy to inactivate the gene. The pKO-AMG plasmid vector contained the bacterial betagalactosidase gene which he used to create an AMG-LacZ gene as an in-frame fusion to exon 2 sequences. Following standard gene replacement methods, he generated a founder mouse strain from brown agouti ES cells a ...
Ch. 8 Power Point
... – Messenger RNA (mRNA) carries the message that will be translated to form a protein. – Ribosomal RNA (rRNA) forms part of ribosomes where proteins are made. – Transfer RNA (tRNA) brings amino acids from the cytoplasm to a ribosome. ...
... – Messenger RNA (mRNA) carries the message that will be translated to form a protein. – Ribosomal RNA (rRNA) forms part of ribosomes where proteins are made. – Transfer RNA (tRNA) brings amino acids from the cytoplasm to a ribosome. ...
Time-Course Analysis of Gene Expression During the
... To identify genes that respond to hypoxia over time, we used two methods: DESeq2 and “AutoCor,” an algorithm developed here. DESeq2 was used to perform a likelihood ratio test (LRT in the DESeq2 package) (Love et al. 2014) that compares how well a gene’s count data fit a “full model” (with independen ...
... To identify genes that respond to hypoxia over time, we used two methods: DESeq2 and “AutoCor,” an algorithm developed here. DESeq2 was used to perform a likelihood ratio test (LRT in the DESeq2 package) (Love et al. 2014) that compares how well a gene’s count data fit a “full model” (with independen ...
Lecture 2: Using Mutants to study Biological processes
... Researchers need both phenotypic and biochemical (protein function) information about their gene to understand its role. The identification of a gene by mutant phenotype = forward genetics -use phenotype to find associated DNA sequence The identification of a gene by DNA sequence -use sequence to fi ...
... Researchers need both phenotypic and biochemical (protein function) information about their gene to understand its role. The identification of a gene by mutant phenotype = forward genetics -use phenotype to find associated DNA sequence The identification of a gene by DNA sequence -use sequence to fi ...
Dol1 Dol3
... • Identify genes associated with survival of WT mice using network analysis that relates a measure of differential expression to connectivity. • Highly connected highly differentially expressed genes have been found to be predictors of survival. ...
... • Identify genes associated with survival of WT mice using network analysis that relates a measure of differential expression to connectivity. • Highly connected highly differentially expressed genes have been found to be predictors of survival. ...
genstat - University of Illinois at Urbana
... University of Illinois, Urbana-Champaign Many slides are taken/adapted from http://www.bioalgorithms.info/slides.htm and Ying Xu’s lecture ...
... University of Illinois, Urbana-Champaign Many slides are taken/adapted from http://www.bioalgorithms.info/slides.htm and Ying Xu’s lecture ...
1 MODULE: Protein-nucleic acid interactions MODULE NUMBER
... Techniques for studying protein-DNA interactions and identifying recognition contacts, including electrophoresis mobility shift assay (EMSA), DNA footprinting and fluorescence anisotropy. These techniques will be introduced using selected biological systems. Techniques for studying protein-DNA inter ...
... Techniques for studying protein-DNA interactions and identifying recognition contacts, including electrophoresis mobility shift assay (EMSA), DNA footprinting and fluorescence anisotropy. These techniques will be introduced using selected biological systems. Techniques for studying protein-DNA inter ...
MUTATION
... be due to accidental cutting by the microtome knife in making his preparations. In 1905 Koernicke treated Lilium with radium and concluded that there was a true fragmentation of the chromosomes. Numerous attempts were made to induce mutations by high-energy radiations and also by other physical and ...
... be due to accidental cutting by the microtome knife in making his preparations. In 1905 Koernicke treated Lilium with radium and concluded that there was a true fragmentation of the chromosomes. Numerous attempts were made to induce mutations by high-energy radiations and also by other physical and ...
Effects of increased concentrations of chloride on the expression of
... oxidative stress has been shown to reduce MnSOD expression, resulting in the accumulation of O2- - particularly in chloroplasts and mitochondria (Liu and Huang, 2000). This decrease in the activity of an isoform of antioxidant enzymes alone does not indicate an inability of the plant to cope with st ...
... oxidative stress has been shown to reduce MnSOD expression, resulting in the accumulation of O2- - particularly in chloroplasts and mitochondria (Liu and Huang, 2000). This decrease in the activity of an isoform of antioxidant enzymes alone does not indicate an inability of the plant to cope with st ...
RNA-Seq
RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.