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RNAi: a defensive RNA-silencing against viruses and transposable
RNAi: a defensive RNA-silencing against viruses and transposable

... whereas siRNAs resulting from an amplification step are 24–26 long. These discoveries highlighted an unexpected complexity of small RNA molecules involved in the RNAi pathway (Hamilton et al, 2002). This begs the question of why transitivity has only been characterized in plants and nematodes to dat ...
Genetics - Biology Teaching & Learning Resources.
Genetics - Biology Teaching & Learning Resources.

... Sperms will carry one copy of the B allele Ova will carry one copy of the b allele When the sperm fertilizes the ovum, the alleles B and b come together in the zygote ...
Document
Document

... Sperms will carry one copy of the B allele Ova will carry one copy of the b allele When the sperm fertilizes the ovum, the alleles B and b come together in the zygote ...
Abstracts
Abstracts

... variability for broadening crop genetic base, and for linking diversity in-situ with ex-situ. Molecular mapping of crop genomic regions associated with traits of interest, jointly with genetic engineering are used to direct the modification of crop plant genomes. The usefulness of these powerful too ...
MS#5_(Cueno and Laude).indd - Philippine Journal of Science
MS#5_(Cueno and Laude).indd - Philippine Journal of Science

You Light Up My Life
You Light Up My Life

... Genome mapping provides basic biological information. ...
ecify proteins via transcription and translation
ecify proteins via transcription and translation

... But a gene does not build a protein directly. The bridge between DNA and protein synthesis is the nucleic acid RNA. You learned in Chapter 5 that RNA is chemically similar to DNA, except that it contains ribose instead of deoxyribose as its sugar and has the nitrogenous base uracil rather than thymi ...
The vertebrate genome annotation (Vega) database
The vertebrate genome annotation (Vega) database

... tagged as being subject to nonsense-mediated decay (NMD) Further more, transcript variants for which a CDS cannot be assigned confidently, are classified into the following main types: (i) Transcript: does not qualify for any of the specific types below. (ii) Retained intron: relative to an appropriate ...
Review of BASIC transmission genetics
Review of BASIC transmission genetics

... m + +!!) m w y and + + + are the Parental (non-recombinant) types. These sets of allele will end up in gametes together most often. Put the 8 progeny types into 4 reciprocal classes. The number of progeny per class is a clue: m w y 3501 ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) e-ISSN: 2278-3008, p-ISSN:2319-7676.
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) e-ISSN: 2278-3008, p-ISSN:2319-7676.

... The protein inclusion is composed of one or more types of delta-endotoxins Cry and Cyt proteins. Many Bacillus thuringiensis with different host spectra have been identified (Burges 1981). The delta-endotoxins are mostly used in agriculture by organic and other growers to control agronomically impor ...
Patients - HAL
Patients - HAL

... CLEEN UP, Amersham Biosciences) were sequenced in both directions, using the BigDye Terminator Cycle Sequencing Ready Reaction kit V1.1. (Applied Biosystems) on an ABI PRISM 3130 capillary sequencer (Applied Biosystems). Sample sequences were aligned to the wild-type ones and analyzed with the Colle ...
HSV-EnV - Trimgen
HSV-EnV - Trimgen

... for the laboratory diagnosis of central nervous system (CNS) diseases caused by these viruses. Rapid laboratory diagnosis is essential for timely treatment of CNS diseases caused by HSV or EnV. A rapid and duplex protocol that covers both HSV and EnV can significantly reduce unnecessary diagnostic t ...
B bab +/ab x ab/ab AB/ABABB ab +/ab x ab/Y AB/ABAB ab +/ab ab
B bab +/ab x ab/ab AB/ABABB ab +/ab x ab/Y AB/ABAB ab +/ab ab

... Recombinant type ...
Bioinformatics - Sequences and Computers
Bioinformatics - Sequences and Computers

... familiar with bioinformatics tools for the analysis of sequences. Language and DNA use sequences to communicate information. The sequence elements in language are letters and punctuation, in DNA they are the nucleotides. As the letters in books contain information that is realized by readers, the se ...
Linkage and Recombination
Linkage and Recombination

... Mendel studied 7 traits and every pair of traits that he reported in his paper segregated independently. Interpretation: are on different chromosomes. Peas have N = 7 chromosomes. Somewhat unlikely that each trait is on a different chromosome. In fact we now know they are not. R (round vs. wrinkled ...
Missense mutations in the PAX6 gene in aniridia.
Missense mutations in the PAX6 gene in aniridia.

... were detected after careful analysis of PCR products from patients' genomic DNA. Patient 1 had two nucleotide substitutions and insertion of a short oligonucleotide on the same allele; nucleotide substitutions from A to G occurred at the 467th and 502nd positions of its cDNA form (in this study, the ...
DNA Profiling - Miss Jan`s Science Wikispace
DNA Profiling - Miss Jan`s Science Wikispace

... e.g. WHY - PCR is used to amplify the DNA sample so that many tests can be taken from the original sample of tissue taken from the victim. HOW – Raising the temperature breaks the hydrogen bonds between the two strands because they are relatively weak bonds. Lowering the temperature anneals the prim ...
Cloning of Hyaluronan Synthase (sz-has) Gene from
Cloning of Hyaluronan Synthase (sz-has) Gene from

... centrifuged at 3,000x g for 20 min. After the cells were removed, 2 volumes of ethanol were added to 1 volume of the supernatant from the culture broth and the solution was refrigerated at 4°C for 1 hr to precipitate the hyaluronic acid. The precipitate was recovered and redissolved in 9% NaCl. The ...
List of Facility Instruments
List of Facility Instruments

... lengths up to 2x250bp. The Illumina HiSeq 1500 is a high-throughput next generation sequencer allowing for massively parallel sequencing technology. This instrument utilizes the v.3 SBS generating up to 500 gigabases (Gb) of data using 2x100bp read length. Creates clonal clusters from single molecul ...
PLEIOTROPIC EFFECT OF Rht3 DWARFING GENE ON SOME
PLEIOTROPIC EFFECT OF Rht3 DWARFING GENE ON SOME

... plant, seed, and flour quality traits were observed in the isogenic lines during two years field experiment planted by 'honey-comb design' at Križevci, Croatia. Significant main effect of Rht3 gene was in shortening of plant height for 54% and 28% in double and single gene dosage respectively. Full- ...
Missense mutations in the PAX6 gene in aniridia.
Missense mutations in the PAX6 gene in aniridia.

... were detected after careful analysis of PCR products from patients' genomic DNA. Patient 1 had two nucleotide substitutions and insertion of a short oligonucleotide on the same allele; nucleotide substitutions from A to G occurred at the 467th and 502nd positions of its cDNA form (in this study, the ...
Molecular Systematics
Molecular Systematics

... • Based on the fact that different alleles for enzymatic proteins may have different electrophoretic mobilities due to differences in protein net charge (due to amino acid differences), or differences in protein conformation. • Many potential differences between genes that will not be detected by th ...
Chapter 15: Chromosomal Basis of Inheritance - Biology E
Chapter 15: Chromosomal Basis of Inheritance - Biology E

... heterozygous for a sex-linked trait, about half her cells will express one allele, while the others will express the alternative allele. 16. Why can you say that all calico cats are females? The tortoiseshell gene is on the X chromosome, and the tortoiseshell phenotype requires the presence of two d ...
Chapter 20: Biotechnology 11/18/2015
Chapter 20: Biotechnology 11/18/2015

... gene expression from each source can be compared in a single microarray (as shown on the slide after next). ...
Partial Linkage
Partial Linkage

... c. How would the results have differed if the genes independently assorted? ...
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RNA-Seq



RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.
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