formerly BISC 180 - GWU Biology Department
... This extensive section will focus on the use of DNA detection methods, such as RFLP, VNTR, STR and SNP for identifying disease alleles. Numerous examples, including Huntingdon’s Disease (HD), cystic fibrosis (CF) and sickle-cell anemia, will be examined to illustrate the principles of these techniqu ...
... This extensive section will focus on the use of DNA detection methods, such as RFLP, VNTR, STR and SNP for identifying disease alleles. Numerous examples, including Huntingdon’s Disease (HD), cystic fibrosis (CF) and sickle-cell anemia, will be examined to illustrate the principles of these techniqu ...
Cystic Fibrosis Transmembrane Conductance Regulator
... was performed on the patients, following PCR amplification of 17 CFTR exons, by denaturing gradient gel electrophoresis (DGGE) and direct genomic sequencing. Surprisingly, the delta F508 mutation was not detected in any of the patients. Instead multiplex amplification of exons 11, 14b, and 17b enabl ...
... was performed on the patients, following PCR amplification of 17 CFTR exons, by denaturing gradient gel electrophoresis (DGGE) and direct genomic sequencing. Surprisingly, the delta F508 mutation was not detected in any of the patients. Instead multiplex amplification of exons 11, 14b, and 17b enabl ...
Multiple Sequence Alignment
... MSAs are alignments of three or more DNA, RNA or protein sequences. Usually theses sequences come from different organisms but sometimes they can be duplicated gene families from the same organism. MSAs have many uses in Bioinformatics. One major use of MSAs is to determine which parts of a sequence ...
... MSAs are alignments of three or more DNA, RNA or protein sequences. Usually theses sequences come from different organisms but sometimes they can be duplicated gene families from the same organism. MSAs have many uses in Bioinformatics. One major use of MSAs is to determine which parts of a sequence ...
statgen9
... but phenotypic in nature. Once again, all possible genotypes must be envisaged. As a general rule, the information available about a family concerns the phenotype. To calculate thelikelihood of , we must envisage all the possible genotype configurations at each of the loci, for this family, writing ...
... but phenotypic in nature. Once again, all possible genotypes must be envisaged. As a general rule, the information available about a family concerns the phenotype. To calculate thelikelihood of , we must envisage all the possible genotype configurations at each of the loci, for this family, writing ...
On the Evolution of Primitive Genetic Codes
... simplified (proto)organism that show that the genetic code can indeed evolve in the presence of strong selection on the encoded polypeptides. This approach differs from previous arguments for the adaptive nature of the code in that we need not assume a direct selection pressure on higher order prope ...
... simplified (proto)organism that show that the genetic code can indeed evolve in the presence of strong selection on the encoded polypeptides. This approach differs from previous arguments for the adaptive nature of the code in that we need not assume a direct selection pressure on higher order prope ...
PowerPoint Slides
... • Data from nearly 40,000 CF patients into the CFTR2 database have been instrumental in: – Increasing the list of clinically, functionally and genetically vetted ‘CF-causing’ mutations from 23 to ~160 (more to follow..) – Providing complete CFTR mutation information on 9 out of 10 patients with CF – ...
... • Data from nearly 40,000 CF patients into the CFTR2 database have been instrumental in: – Increasing the list of clinically, functionally and genetically vetted ‘CF-causing’ mutations from 23 to ~160 (more to follow..) – Providing complete CFTR mutation information on 9 out of 10 patients with CF – ...
Genome Science performed with e-Science Tools
... extended into these “gap” regions in order to produce a complete and accurate map of the WBSCR. Once placed in this region, these DNA sequences were analysed with a battery of prediction tools in order to locate putative genes and regulatory elements possibly implicated in the disorder. Finally, any ...
... extended into these “gap” regions in order to produce a complete and accurate map of the WBSCR. Once placed in this region, these DNA sequences were analysed with a battery of prediction tools in order to locate putative genes and regulatory elements possibly implicated in the disorder. Finally, any ...
Ch 11 Mendel STUDENT lecture notes
... there were several traits of peas, such as color, smoothness, height, and flower color. Sometimes he would allow the flower to ___________ pollinate. Most flowers have both sperm (sperm) and egg. He called this________ ___________________. The offspring of this true breeding always brought about off ...
... there were several traits of peas, such as color, smoothness, height, and flower color. Sometimes he would allow the flower to ___________ pollinate. Most flowers have both sperm (sperm) and egg. He called this________ ___________________. The offspring of this true breeding always brought about off ...
Document
... - many different sex-determining systems in plants and animals with separate sexes. ...
... - many different sex-determining systems in plants and animals with separate sexes. ...
Physicochemical studies on interactions between DNA and RNA
... by the triangles. The filled circles give the absorbance difference measured at 37°C. The vertical bar in the figure represents the noise level of the ...
... by the triangles. The filled circles give the absorbance difference measured at 37°C. The vertical bar in the figure represents the noise level of the ...
Greedy Algorithms And Genome Rearrangements
... Turnip vs Cabbage: Almost Identical mtDNA gene sequences • In 1980s Jeffrey Palmer studied evolution of plants by comparing genomes of the cabbage and turnip • 99% similarity between genes • These surprisingly identical gene sequences differed in gene order • This study helped pave the way to analyz ...
... Turnip vs Cabbage: Almost Identical mtDNA gene sequences • In 1980s Jeffrey Palmer studied evolution of plants by comparing genomes of the cabbage and turnip • 99% similarity between genes • These surprisingly identical gene sequences differed in gene order • This study helped pave the way to analyz ...
`Candidatus Midichloria mitochondrii`, an endosymbiont of the tick
... Most of the above tick-associated 16S rRNA gene sequences were obtained in the context of PCR surveys without any specific microscopical observations. One exception was the study by Beninati et al. (2004) that reported electron microscopy (EM) observations of an intracellular bacterium as well as in ...
... Most of the above tick-associated 16S rRNA gene sequences were obtained in the context of PCR surveys without any specific microscopical observations. One exception was the study by Beninati et al. (2004) that reported electron microscopy (EM) observations of an intracellular bacterium as well as in ...
Slide 1
... formation of cystine calculi in the kidneys due to low solubility of cystine in acidic environment. Clinically, cystinuria is divided into two types: • Type I cystinuria – heterozygotes have normal excretion of cystine and dibasic amino acids, which implies that the disease is inherited autosomal-re ...
... formation of cystine calculi in the kidneys due to low solubility of cystine in acidic environment. Clinically, cystinuria is divided into two types: • Type I cystinuria – heterozygotes have normal excretion of cystine and dibasic amino acids, which implies that the disease is inherited autosomal-re ...
Rebuttal - MIT Technology Review
... 1) SENS treats symptoms, not causes (stated both directly and using the analogy of diabetes) On the contrary, the symptoms of aging (age-related diseases and debility) are not targets of SENS: rather, SENS targets their accumulating and initially inert precursors (“damage”), including indigestible m ...
... 1) SENS treats symptoms, not causes (stated both directly and using the analogy of diabetes) On the contrary, the symptoms of aging (age-related diseases and debility) are not targets of SENS: rather, SENS targets their accumulating and initially inert precursors (“damage”), including indigestible m ...
The HapMap Project Tutorial
... 10 million SNPs in the Human genome 300k-600k tag SNPs that can contain the information of the 10 million (these SNPs are the most important for HapMap to catalog) ...
... 10 million SNPs in the Human genome 300k-600k tag SNPs that can contain the information of the 10 million (these SNPs are the most important for HapMap to catalog) ...
Worksheet 2
... What chromosome is the amplicon located on? ____ Click on the number underneath the tagged chromosome. Zoom all the way out. How long is the chromosome? _____ Mbp (Mega bp) or _______________ cM Where about on the chromosome is the bronze locus located? _______________ Zoom step-wise into the chromo ...
... What chromosome is the amplicon located on? ____ Click on the number underneath the tagged chromosome. Zoom all the way out. How long is the chromosome? _____ Mbp (Mega bp) or _______________ cM Where about on the chromosome is the bronze locus located? _______________ Zoom step-wise into the chromo ...
Concept Check Questions
... Beth and Tom each have a sibling with cystic fibrosis, but neither Beth nor Tom nor any of their parents have the disease. Calculate the probability that if this couple has a child, the child will have cystic fibrosis. What would be the probability if a test revealed that Tom is a carrier but Beth i ...
... Beth and Tom each have a sibling with cystic fibrosis, but neither Beth nor Tom nor any of their parents have the disease. Calculate the probability that if this couple has a child, the child will have cystic fibrosis. What would be the probability if a test revealed that Tom is a carrier but Beth i ...
persist. Fruit flies, on the other hand, models of speciation, genetic
... the adaptive mutations identified in other evolution experiments were chromosomal rearrangements that duplicated genes known to be involved in the uptake or metabolism of a limiting nutrient, or altered their regulation [8]. Preventing incompatibility within a lineage by making such rearrangements h ...
... the adaptive mutations identified in other evolution experiments were chromosomal rearrangements that duplicated genes known to be involved in the uptake or metabolism of a limiting nutrient, or altered their regulation [8]. Preventing incompatibility within a lineage by making such rearrangements h ...
IV RNA Synthesis: Transcription
... chemistry of a nucleic acid; enzymes that act on DNA usually have no effect on RNA, and vice versa. However, the change from thymine to uracil does not affect base pairing, as these two bases pair with adenine equally well. RNA plays several important roles in the cell. Three major types of RNA are ...
... chemistry of a nucleic acid; enzymes that act on DNA usually have no effect on RNA, and vice versa. However, the change from thymine to uracil does not affect base pairing, as these two bases pair with adenine equally well. RNA plays several important roles in the cell. Three major types of RNA are ...
LINKAGE - TYPES OF LINKAGE AND ESTIMATION OF LINKAGE
... The genes distantly located in the chromosome show incomplete linkage because they have a chance of separation by crossing over and of going into different gametes and offspring. Importance of linkage in breeding When there is a close linkage between desirable and undesirable characters these genes ...
... The genes distantly located in the chromosome show incomplete linkage because they have a chance of separation by crossing over and of going into different gametes and offspring. Importance of linkage in breeding When there is a close linkage between desirable and undesirable characters these genes ...
Bayesian Partition Models for Identifying Expression Quantitative
... certain genes. By assaying gene expression and genetic variation (e.g., SNPs and/or copy number variations (CNVs)) simultaneously in segregating populations, scientists wish to correlate variations in the gene expression with genomic sequence variations. In such cases we say that a gene’s expression ...
... certain genes. By assaying gene expression and genetic variation (e.g., SNPs and/or copy number variations (CNVs)) simultaneously in segregating populations, scientists wish to correlate variations in the gene expression with genomic sequence variations. In such cases we say that a gene’s expression ...
emboj2008205-sup
... of expansions and contractions of repeat tracts during mitotic divisions, we re-streaked yeast colonies that have been verified for the presence of (GAA)340 full size repeats on complete media. Ten colonies were then selected for PCR amplification to look for changes in the length of the repetitive ...
... of expansions and contractions of repeat tracts during mitotic divisions, we re-streaked yeast colonies that have been verified for the presence of (GAA)340 full size repeats on complete media. Ten colonies were then selected for PCR amplification to look for changes in the length of the repetitive ...
RNA-Seq
RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.