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plotfold
plotfold

... structure for an RNA or DNA molecule depends on the model of folding and the specific folding energies used to calculate that structure. Different optimal foldings may be calculated if the folding energies are changed even slightly. Because of uncertainties in the folding model and the folding energ ...
Identification of genes involved in the same
Identification of genes involved in the same

... functional modules are to cluster these genes using hierarchical clustering techniques (Eisen, 1998; Grotkjær et al., 2006), k-means clustering (Tavazoie, 1999), techniques such as Self Organizing Maps (SOM) (Tamayo et al., 1999), or combinations of these techniques (Herrero and Dopazo, 2002). While ...
Bacterial and Firefly Luciferase Genes in Transgenic Plants
Bacterial and Firefly Luciferase Genes in Transgenic Plants

... assays, such as determination of the concentration of O,, anaesthetics, antibiotics, mutagens, etc., can be carried out in vivo by expression of luciferases in living cells [Hastings and Nealson, 19771. Cloning of luciferase genes opened the way to novel applications in molecular biology. Gene fusio ...
The evolution of genomic imprinting and X
The evolution of genomic imprinting and X

... retained it even after further genome rearrangements deposited it upon an autosome. ...
Aptamers as Drugs. PDF
Aptamers as Drugs. PDF

BI:4224
BI:4224

... lipids and proteins, causing them to precipitate out of the solution. 2. What is the role of sodium chloride in the lysing solution? Answer: Sodium chloride in the lysing solution reacts with the negative phosphate ends of the DNA, causing adjacent nucleic acids molecules to ...
A candidate prostate cancer susceptibility gene at
A candidate prostate cancer susceptibility gene at

... added an additional 94 families, making a total of 127 (Table 2); these were typed for markers at both this and the HPC1 locus. The overall data set neither provides significant lod score evidence for linkage on chromosome 17p nor provides sufficient evidence for de novo identification of the HPC1 l ...
Historical review: Deciphering the genetic code – a personal account
Historical review: Deciphering the genetic code – a personal account

... lacks cysteine so that I could explore mechanisms of gene regulation. I thought that in the absence of cysteine the synthesis of penicillinase might proceed, whereas synthesis of most other proteins might be reduced. In England, Pollock [2] had shown that penicillinase is inducible in Bacillus cerus ...
IMPROVE SMALL RNA-MEDIATED GENE SILENCING
IMPROVE SMALL RNA-MEDIATED GENE SILENCING

... We now have a much greater understanding of the gene silencing pathways in plants and their crucial roles in a variety of biological regulation processes, such as development, plant defense against invading viral nucleic acids, and epigenetic modifications. siRNA directed target repression can occur ...
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... • Dump NRDB records from GUS to FASTA file - with GUS Ids ...
Using Ontology Graphs to Understand Annotations and Reason about Them
Using Ontology Graphs to Understand Annotations and Reason about Them

... Figure 7. Detail of the Mammalian Phenotype (MP) Ontology annotation graph for two alleles of mouse gene Arx representing allelic compositions Arxtm1Kki/Y (blue/lighter shading) and Arxtm1Pgr/Y (red/darker shading). We observe that the allele annotations segregate in separate ontology branches. Onl ...
Slide 1
Slide 1

... 2. Why is the fate of most duplicate genes to rates, compared to angiosperms? Or, on eventually become silenced? Could the other hand, could the silenced genes mutations accumulate in both copies at the hold the key to the long history of fern same rate causing subfunctionalization, evolution? where ...
PDF - European Journal of Biotechnology and Bioscience
PDF - European Journal of Biotechnology and Bioscience

... predicted to be the primary cause of the long term gene expression. In contrast to adenoviruses, most people treated with AAV will not build an immune response to remove the virus and the cells that have been successfully treated with it. Several trials with AAV are on-going or in preparation, mainl ...
The epigenetic basis of gender in flowering plants and mammals
The epigenetic basis of gender in flowering plants and mammals

... What makes a sperm male or an egg female, and how can we tell? A gamete’s gender could be defined in many ways, such as the sex of the individual or organ that produced it, its cellular morphology, or its behaviour at fertilization. In flowering plants and mammals, however, there is an extra dimensi ...
The varied roles of nuclear receptors during vertebrate embryonic
The varied roles of nuclear receptors during vertebrate embryonic

... receptors are specifically expressed and perform specific functions [Thummel, 1995]. Seven of them are orphan receptors, for which ligands have not yet been identified. This phenomenon suggests nuclear receptors play an important role in insect embryonic development. At the end of the third instar l ...
Genes Are DNA
Genes Are DNA

... 1.12 Mutations May Affect Single Base Pairs or Longer Sequences ...
Apoptosis: Translating Theory to Therapy for Prostate Cancer
Apoptosis: Translating Theory to Therapy for Prostate Cancer

... undergo apoptosis by a variety of agents that initiate the apoptotic cascade distal to the point regulated by AR in androgendependent prostate cancer cells (13–16). Unfortunately, however, by initiating the apoptotic cascade downstream, there is essentially no therapeutic index for the cytotoxic res ...
Identification of the 5T‐12TG allele of the cystic fibrosis
Identification of the 5T‐12TG allele of the cystic fibrosis

... ligation assay (OLA) [10] during the screening proceedings. Subsequently, genomic DNA was extracted from peripheral whole blood samples by standard methods. PCR and denaturing high-performance liquid chromatography (DHPLC) analyses were performed using primers and protocols previously described [11] ...
DNA copy number analysis by MAPH: molecular diagnostic
DNA copy number analysis by MAPH: molecular diagnostic

... locus at a chromosome end, is shown as a peak. The first sam- BRCA1 or subtelomeric DNA analysis, however, the exon or ple is a heterozygous deletion of 17q (17q-) and the second is chromosome end that varies, if any, is not known. This can be an unbalanced translocation between chromosomes 16 and 3 ...
Jhe in Gryllus assimilis: Cloning, sequence
Jhe in Gryllus assimilis: Cloning, sequence

Causes, Risks, Prevention
Causes, Risks, Prevention

... rather than having been inherited. These acquired gene mutations sometimes result from exposure to cancer-causing chemicals (like those found in tobacco smoke). But often what causes these changes is not known. Many gene changes are probably just random events that sometimes happen inside a cell, wi ...
Codon - Cloudfront.net
Codon - Cloudfront.net

... Practice Problem Always use mRNA to find the amino acid ...
Nucleotide sequence diversity at the alcohol
Nucleotide sequence diversity at the alcohol

... background selection hypothesis by studying a predominantly self-fertilizing grass species that could be contrasted to the two outcrossing (wind pollinated) grass species already investigated, and (ii) increase statistical power by sequencing a large sample of adh1 genes drawn from throughout the sp ...
AI - tcurry1977
AI - tcurry1977

... Part III • Trait Analysis 1. The following pedigrees will be used to determine whether the trait is autosomal dominant or autosomal recessive. In tracing autosomal alleles, if both parents have the disorder and the offspring do not, the condition is autosomal dominant. If neither parent shows the d ...
gene technology extra qs with mark scheme
gene technology extra qs with mark scheme

... The inheritance of the ability to produce hydrogen cyanide is controlled by two genes which are located on different chromosomes. The dominant allele of one gene, G, controls the production of enzyme G which converts a precursor to linamarin. The dominant allele of the other gene, E, controls the pr ...
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RNA-Seq



RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.
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