letters Solution structure of the DNA-binding domain of MafG
... The Maf family of transcription factors are basic regionleucine zipper (bZIP) proteins, characterized by the presence of a specific amino acid sequence, the Maf extended homology ...
... The Maf family of transcription factors are basic regionleucine zipper (bZIP) proteins, characterized by the presence of a specific amino acid sequence, the Maf extended homology ...
Biophysics 101 Genomics and Computational Biology
... Knegtel et al J Comput Aided Mol Des 1999 13:167-83 Comparison of two implementations of the incremental construction algorithm in flexible docking of thrombin inhibitors. A set of 32 known thrombin inhibitors representing different chemical classes has been used to evaluate the performance of two i ...
... Knegtel et al J Comput Aided Mol Des 1999 13:167-83 Comparison of two implementations of the incremental construction algorithm in flexible docking of thrombin inhibitors. A set of 32 known thrombin inhibitors representing different chemical classes has been used to evaluate the performance of two i ...
Molecular Analysis of the Prostacyclin Receptor’s Interaction with
... members of the somatostatin receptor (SSTR) family) [10,11,12,13,14]. In addition, through its interaction with the high density lipoprotein (HDL) scavenger receptor class B, type I (SR-BI), PDZK1 is essential for both reverse cholesterol transport ...
... members of the somatostatin receptor (SSTR) family) [10,11,12,13,14]. In addition, through its interaction with the high density lipoprotein (HDL) scavenger receptor class B, type I (SR-BI), PDZK1 is essential for both reverse cholesterol transport ...
Chapter 1
... proteoliposomes in which the LacS protein is functionally incorporated in the lipid bilayer (72, 73). The development of this methodology has been crucial for many of the studies described in this thesis. One of the essential features is the fact that reconstitution proceeds unidirectionally, that i ...
... proteoliposomes in which the LacS protein is functionally incorporated in the lipid bilayer (72, 73). The development of this methodology has been crucial for many of the studies described in this thesis. One of the essential features is the fact that reconstitution proceeds unidirectionally, that i ...
The ACT Domain: A Small Molecule Binding Domain
... is particularly interesting because it contains an ACT domain followed closely, within the same chain, by a ␣ fold that together forms a seven-stranded  sheet (19). Mutagenesis studies (20) indicate that the effector ligand (isoleucine) binding site is located at the interface between these two ...
... is particularly interesting because it contains an ACT domain followed closely, within the same chain, by a ␣ fold that together forms a seven-stranded  sheet (19). Mutagenesis studies (20) indicate that the effector ligand (isoleucine) binding site is located at the interface between these two ...
Protein synthesis and degradation in the liver
... majority of proteins is facilitated by the translocating chainassociating membrane (TRAM), a multispanning glycoprotein that aids the formation of a tight ribosome–membrane junction through a signal sequence-dependent process [10]. In addition, the translocon complex interacts with the ribosome, the ...
... majority of proteins is facilitated by the translocating chainassociating membrane (TRAM), a multispanning glycoprotein that aids the formation of a tight ribosome–membrane junction through a signal sequence-dependent process [10]. In addition, the translocon complex interacts with the ribosome, the ...
Getting a good rate of exchange – the mitochondrial ADP
... of TM α-helices and lie adjacent to the inner surface of the membrane (view-4). These smaller αhelices are called the matrix helices as they lie in the interior or “matrix” side of the mitochondrial inner membrane. The pore structure therefore consists of a sequence of three structurally similar rep ...
... of TM α-helices and lie adjacent to the inner surface of the membrane (view-4). These smaller αhelices are called the matrix helices as they lie in the interior or “matrix” side of the mitochondrial inner membrane. The pore structure therefore consists of a sequence of three structurally similar rep ...
Khristy Nicholas - A History of the Development of Anthrax Vaccines and a Look into the Future
... use only, except in Russia. Recently, because of the advances on recombinant DNA technology, there has been great improvement in a spore related vaccine, but non- toxic! (Kaur & Bhatnagar, 2011). One of the major new discoveries was that “… spore antigens can be used to augment immunity against anth ...
... use only, except in Russia. Recently, because of the advances on recombinant DNA technology, there has been great improvement in a spore related vaccine, but non- toxic! (Kaur & Bhatnagar, 2011). One of the major new discoveries was that “… spore antigens can be used to augment immunity against anth ...
View PDF - CiteSeerX
... MCGGAII/L and cannot be assigned to any of the three previously de¢ned ERF classes [8]. This N-terminal motif is only found in ERF genes, including AtEBP from Arabidopsis [23] and OsEBP-89 from rice [20]. LeERF2, therefore, de¢nes the new ERF class IV. Though the function of the MCGGAII/L motif has ...
... MCGGAII/L and cannot be assigned to any of the three previously de¢ned ERF classes [8]. This N-terminal motif is only found in ERF genes, including AtEBP from Arabidopsis [23] and OsEBP-89 from rice [20]. LeERF2, therefore, de¢nes the new ERF class IV. Though the function of the MCGGAII/L motif has ...
Mrs C`s Chem Lecture
... covalent bond. This “cross-linking” helps stabilize protein structure. Cysteine Cysteine is an important sulfur-containing amino acid. ...
... covalent bond. This “cross-linking” helps stabilize protein structure. Cysteine Cysteine is an important sulfur-containing amino acid. ...
PDF File
... prokaryotes and eukaryotes (2– 8). It is likely, therefore, that active extrusion systems play a crucial role in the cellular defense mechanism against incoming noxious compounds in many living organisms. It is of great interest and importance, therefore, to analyze the mechanism by which such unive ...
... prokaryotes and eukaryotes (2– 8). It is likely, therefore, that active extrusion systems play a crucial role in the cellular defense mechanism against incoming noxious compounds in many living organisms. It is of great interest and importance, therefore, to analyze the mechanism by which such unive ...
Crystal Structure of the Carboxyltransferase Domain of Acetyl
... way that the N domain of one molecule is placed next to the C domain of the other (Fig. 1C). The ␣6A-␣6D insertion in the C domain of one monomer (Fig. 2B) is interdigitated between the 7A-7D insertion (Fig. 2A) and the core of the N domain of the other monomer (Fig. 1C). The insertion between 4 ...
... way that the N domain of one molecule is placed next to the C domain of the other (Fig. 1C). The ␣6A-␣6D insertion in the C domain of one monomer (Fig. 2B) is interdigitated between the 7A-7D insertion (Fig. 2A) and the core of the N domain of the other monomer (Fig. 1C). The insertion between 4 ...
The structure and function of proline
... tannin-binding protein described above. They are large proteins, with a long tandemly repeated section. For example, the human tumour-associated polymorphic epithelial mucin has a 20-residue proline-rich sequence repeated between 21 and 125 times [41]. It is heavily glycosylated and is thought to fu ...
... tannin-binding protein described above. They are large proteins, with a long tandemly repeated section. For example, the human tumour-associated polymorphic epithelial mucin has a 20-residue proline-rich sequence repeated between 21 and 125 times [41]. It is heavily glycosylated and is thought to fu ...
Barbara Soldo
... PPi Release Activity in the Presence of Amino-acyl Acceptor. In order to monitor the complete A-domain catalyzed reaction, we attempted to measure acceleration of PPi release as a result of interdomain transfer of aminoacyl to TycA holo-PCP domain added in trans. TycA PCP domain was coexpressed toge ...
... PPi Release Activity in the Presence of Amino-acyl Acceptor. In order to monitor the complete A-domain catalyzed reaction, we attempted to measure acceleration of PPi release as a result of interdomain transfer of aminoacyl to TycA holo-PCP domain added in trans. TycA PCP domain was coexpressed toge ...
fae04be7f127386
... speed up the rate at which GTPases hydrolyze GTP, and hence the rate at which they inactivate, while guanine nucleotide exchange factors, or GEFs, assist in the exchange of GDP for GTP and therefore help GTPases adopt their ...
... speed up the rate at which GTPases hydrolyze GTP, and hence the rate at which they inactivate, while guanine nucleotide exchange factors, or GEFs, assist in the exchange of GDP for GTP and therefore help GTPases adopt their ...
Analysis of hepatocyte nuclear factor
... requires conserved sequences in regions IV and V Although we had extensively analyzed the HNF-3, C-terminal transcriptional activation domain (46), the sequence requirement for activity of the HNF-3I N-terminal domain warranted further investigation. We prepared HNF-3p expression constructs for synt ...
... requires conserved sequences in regions IV and V Although we had extensively analyzed the HNF-3, C-terminal transcriptional activation domain (46), the sequence requirement for activity of the HNF-3I N-terminal domain warranted further investigation. We prepared HNF-3p expression constructs for synt ...
Recitation 2 - MIT OpenCourseWare
... Bonding: There are many types of bonds that hold molecules together. Covalent bonds result when two atoms share a pair of electrons between them, such as the bonds between C and H in methane. Ionic bonds occur between atoms with a very high difference in electronegativities. Hydrogen bonds occur bet ...
... Bonding: There are many types of bonds that hold molecules together. Covalent bonds result when two atoms share a pair of electrons between them, such as the bonds between C and H in methane. Ionic bonds occur between atoms with a very high difference in electronegativities. Hydrogen bonds occur bet ...
Tertiary Structure
... residues that are structurally equivalent to positions in 9 known globin structures, that are involved in helix-heme contacts, and in the packing of the helices against each other. – There are a total of 59 positions preserved, 31 buried in the middle of protein and 28 in contact with the heme group ...
... residues that are structurally equivalent to positions in 9 known globin structures, that are involved in helix-heme contacts, and in the packing of the helices against each other. – There are a total of 59 positions preserved, 31 buried in the middle of protein and 28 in contact with the heme group ...
Functions of Ribosome-Associated Chaperones and their Interaction
... gene tig does not cause any growth defect albeit the heat shock response is induced leading to enhanced levels of chaperones and proteases [32, 33]. First evidence that TF fulfills chaperone function in vivo came from the finding that the simultaneous deletion of TF and the cytosolic Hsp70 chaperone ...
... gene tig does not cause any growth defect albeit the heat shock response is induced leading to enhanced levels of chaperones and proteases [32, 33]. First evidence that TF fulfills chaperone function in vivo came from the finding that the simultaneous deletion of TF and the cytosolic Hsp70 chaperone ...
Moving Proteins into Membranes and Organelles Moving Proteins
... NPCs span the inner and outer nuclear membrane – 3000-4000 in typical mammalian cell nucleus. – Each pore, on average, imports 100 histone molecules per minute and exports 6 small ribosomal subunits. The formed protein also called nucleoporin ...
... NPCs span the inner and outer nuclear membrane – 3000-4000 in typical mammalian cell nucleus. – Each pore, on average, imports 100 histone molecules per minute and exports 6 small ribosomal subunits. The formed protein also called nucleoporin ...
The X-ray Crystal Structures of Human
... both structurally and mechanistically from the phosphomanno/phosphoglucomutase enzymes of the phosphohexomutase superfamily that are integral to glycolysis in prokaryotes and eukaryotes (15) and to alginate biosynthesis in Gram-negative bacteria (16). Within the HADSF, ␣-PMM is distinguished from th ...
... both structurally and mechanistically from the phosphomanno/phosphoglucomutase enzymes of the phosphohexomutase superfamily that are integral to glycolysis in prokaryotes and eukaryotes (15) and to alginate biosynthesis in Gram-negative bacteria (16). Within the HADSF, ␣-PMM is distinguished from th ...
Structural Biology: What does 3D tell us?
... are known as motifs or supersecondary structures A motif is usually smaller than a domain but can encompass an entire domain. Sometimes the structures of domains are partly named after motifs that they contain, e.g. “greek key beta barrel” It should be noted that the term motif, when used in conjunc ...
... are known as motifs or supersecondary structures A motif is usually smaller than a domain but can encompass an entire domain. Sometimes the structures of domains are partly named after motifs that they contain, e.g. “greek key beta barrel” It should be noted that the term motif, when used in conjunc ...
CAMPYLOBACTER
... placed on HeLa cells and CHO cells and showed significant cytotoxicity similar to Shiga-like toxin but not neutralized by antisera against either Shiga-like toxin I or II. This cytotoxin was unstable at temperature above 50C and its activity decreased by the treatment of trypsin. This cytotoxin ...
... placed on HeLa cells and CHO cells and showed significant cytotoxicity similar to Shiga-like toxin but not neutralized by antisera against either Shiga-like toxin I or II. This cytotoxin was unstable at temperature above 50C and its activity decreased by the treatment of trypsin. This cytotoxin ...
uptake of nutrients-2014
... on the external face of the gram-positive plasma membrane. These binding proteins, which also may participate in chemotaxis (see pp. 66–68), bind the molecule to be transported and then interact with the membrane transport proteins to move the solute molecule inside the cell. E. coli transports a va ...
... on the external face of the gram-positive plasma membrane. These binding proteins, which also may participate in chemotaxis (see pp. 66–68), bind the molecule to be transported and then interact with the membrane transport proteins to move the solute molecule inside the cell. E. coli transports a va ...
Anthrax toxin
Anthrax toxin is a three-protein exotoxin secreted by virulent strains of the bacterium, Bacillus anthracis—the causative agent of anthrax. The toxin was first discovered by Harry Smith in 1954. Anthrax toxin is composed of a cell-binding protein, known as protective antigen (PA), and two enzyme components, called edema factor (EF) and lethal factor (LF). These three protein components act together to impart their physiological effects. Assembled complexes containing the toxin components are endocytosed. In the endosome, the enzymatic components of the toxin translocate into the cytoplasm of a target cell. Once in the cytosol, the enzymatic components of the toxin disrupts various immune cell functions, namely cellular signaling and cell migration. The toxin may even induce cell lysis, as is observed for macrophage cells. Anthrax toxin allows the bacteria to evade the immune system, proliferate, and ultimately kill the host animal. Research on anthrax toxin also provides insight into the generation of macromolecular assemblies, and on protein translocation, pore formation, endocytosis, and other biochemical processes.