X-ray crystallography electron microscopy and electron diffraction
... b) The calculated Fourier transform of the crystal in a) shows clear diffraction spots, extending to 5 orders in one direction and 7 in the other. The crystallographic resolution is thus 137Å/5 = 27 Å along h and 174Å/7 = 25 Å along k. The diffraction spots are indexed along h and k as shown by the ...
... b) The calculated Fourier transform of the crystal in a) shows clear diffraction spots, extending to 5 orders in one direction and 7 in the other. The crystallographic resolution is thus 137Å/5 = 27 Å along h and 174Å/7 = 25 Å along k. The diffraction spots are indexed along h and k as shown by the ...
GFP Introduction Powerpoint Presentation - mvhs
... • Achieved through the use of selectable markers • Selectable markers = traits that help identify a cell with the plasmid in it (compared to one without it) • In our experiment, the bla gene will serve as the selectable marker Images taken without permission from http://www.antibioresistance.be/Gifs ...
... • Achieved through the use of selectable markers • Selectable markers = traits that help identify a cell with the plasmid in it (compared to one without it) • In our experiment, the bla gene will serve as the selectable marker Images taken without permission from http://www.antibioresistance.be/Gifs ...
Inhibition of Protein Kinases by Balanol: Specificity within the Serine
... ring (Bal-7R) on PKA is 47.3 6 1.1 nM (mean 6 S.E.; n 5 2; Fig. 2B). The Ki values for 140-decarboxybalanol and 100deoxybalanol on PKA are 11 and 3.9 nM (Koide et al., 1995). Thus, eliminating the seven-membered ring from balanol backbone has a modest effect on balanol’s affinity toward the ATP bind ...
... ring (Bal-7R) on PKA is 47.3 6 1.1 nM (mean 6 S.E.; n 5 2; Fig. 2B). The Ki values for 140-decarboxybalanol and 100deoxybalanol on PKA are 11 and 3.9 nM (Koide et al., 1995). Thus, eliminating the seven-membered ring from balanol backbone has a modest effect on balanol’s affinity toward the ATP bind ...
Site directed mutagenesis of Drosophila flightin disrupts
... (Kronert et al. 1995), indicate that the myosin rod represents the primary binding site for flightin in the sarcomere. A recent study (Flashman et al. 2007) has shown that the CX myosin binding domain of vertebrate myosin binding protein C (MyBP-C) binds to the light meromyosin (LMM) region between ...
... (Kronert et al. 1995), indicate that the myosin rod represents the primary binding site for flightin in the sarcomere. A recent study (Flashman et al. 2007) has shown that the CX myosin binding domain of vertebrate myosin binding protein C (MyBP-C) binds to the light meromyosin (LMM) region between ...
- Wiley Online Library
... the entry of carbohydrates either in phosphorylated or non-phosphorylated form, a distinction that is especially important for CCR. Carbohydrate transport and concomitant phosphorylation is achieved by components of the phosphoenolpyruvate (PEP)-dependent carbohydrate phosphotransferase system (PTS) ...
... the entry of carbohydrates either in phosphorylated or non-phosphorylated form, a distinction that is especially important for CCR. Carbohydrate transport and concomitant phosphorylation is achieved by components of the phosphoenolpyruvate (PEP)-dependent carbohydrate phosphotransferase system (PTS) ...
Getting the message across: how do plant cells exchange
... Although Rab proteins are generally associated with the regulation of vesicle-mediated transport [22], they might also play a role in trafficking large ribonucleoprotein complexes to the cell periphery. For example, in the Drosophila oocyte, Rab11 has been shown to be involved in highly polarized mR ...
... Although Rab proteins are generally associated with the regulation of vesicle-mediated transport [22], they might also play a role in trafficking large ribonucleoprotein complexes to the cell periphery. For example, in the Drosophila oocyte, Rab11 has been shown to be involved in highly polarized mR ...
BioExpress® 6000 Mammalian Cell Growth Media
... H; 15N HSQC spectrum plotted at different threshhold levels of 200 μM sample of rhodospin expressed in CIL’s 15N-labeled media. The tryptophan sidechain signals are clearly visible, and the protein is folded in its native conformation. The broad peaks observed are expected for a large membrane prote ...
... H; 15N HSQC spectrum plotted at different threshhold levels of 200 μM sample of rhodospin expressed in CIL’s 15N-labeled media. The tryptophan sidechain signals are clearly visible, and the protein is folded in its native conformation. The broad peaks observed are expected for a large membrane prote ...
Intrinsically Disordered Proteins (IDPs)
... Proteins (IDPs) and ID Regions (IDRs) • Whole proteins and regions of proteins are intrinsically disordered if they lack stable 3D structure under physiological conditions, • But exist instead as highly dynamic, rapidly interconverting ensembles without particular equilibrium values for their coordi ...
... Proteins (IDPs) and ID Regions (IDRs) • Whole proteins and regions of proteins are intrinsically disordered if they lack stable 3D structure under physiological conditions, • But exist instead as highly dynamic, rapidly interconverting ensembles without particular equilibrium values for their coordi ...
![HydF as a scaffold protein in [FeFe] hydrogenase H](http://s1.studyres.com/store/data/022194833_1-d80dffc73c0d81a42175e12a27187746-300x300.png)
HydF as a scaffold protein in [FeFe] hydrogenase H
... [FeFe] hydrogenases. Recent studies involving the analysis of mutants of Chlamydomonas reinhardtii defective in hydrogen production have revealed that products of the hydEF and hydG genes are required for the accumulation of active [FeFe] hydrogenase, and that hydE, hydF, and hydG are common to all ...
... [FeFe] hydrogenases. Recent studies involving the analysis of mutants of Chlamydomonas reinhardtii defective in hydrogen production have revealed that products of the hydEF and hydG genes are required for the accumulation of active [FeFe] hydrogenase, and that hydE, hydF, and hydG are common to all ...
Biocatalytic Synthesis of Polymers of Precisely Defined Structures
... Polypeptides also differ from most synthetic polymers in that they adopt ordered threedimensional structures in solution and in the solid state. Most naturally occuning proteins adopt globular structures consistent with their roles in substrate recognition and transformation, for example, as enzymes ...
... Polypeptides also differ from most synthetic polymers in that they adopt ordered threedimensional structures in solution and in the solid state. Most naturally occuning proteins adopt globular structures consistent with their roles in substrate recognition and transformation, for example, as enzymes ...
Connective tissue
... an aminopeptidase. This combination of collagenolytic and proteolytic activities is effective at breaking down intercellular matrices, the essential part of tissue dissociation. One component of the complex is a hydrolytic enzyme which degrades the helical regions in native collagen preferentially a ...
... an aminopeptidase. This combination of collagenolytic and proteolytic activities is effective at breaking down intercellular matrices, the essential part of tissue dissociation. One component of the complex is a hydrolytic enzyme which degrades the helical regions in native collagen preferentially a ...
BS3 Crosslinking
... BS3 Crosslinking Assay Bis (sulfosuccinimidyl) suberate (BS3) crosslinking was performed as described previously (Grosshans et al., 2001, 2002; Conrad et al., 2008). BS3 is a membrane-impermeable agent, which selectively crosslinks cell-surface proteins to form high-molecular-mass aggregates. Becaus ...
... BS3 Crosslinking Assay Bis (sulfosuccinimidyl) suberate (BS3) crosslinking was performed as described previously (Grosshans et al., 2001, 2002; Conrad et al., 2008). BS3 is a membrane-impermeable agent, which selectively crosslinks cell-surface proteins to form high-molecular-mass aggregates. Becaus ...
PDF
... A search of the S. cerevisiae proteome found 553 occurrences of this consensus sequence in 491 proteins (Table 2, which is published as supporting information on the PNAS web site). Fifty-one proteins were found to have multiple sites, with 5 sites being the most present in any one protein. The seco ...
... A search of the S. cerevisiae proteome found 553 occurrences of this consensus sequence in 491 proteins (Table 2, which is published as supporting information on the PNAS web site). Fifty-one proteins were found to have multiple sites, with 5 sites being the most present in any one protein. The seco ...
Receptor-mediated signaling at plasmodesmata
... the SPFH domain protein AtHIR2 (Qi and Katagiri, 2012), pro- a direct link between PDLP5 and callose synthases. Whether viding further evidence that FLS2 activity associates with lipid this link comes from direct complex formation between these rafts. This allows speculation that FLS2 activity, and ...
... the SPFH domain protein AtHIR2 (Qi and Katagiri, 2012), pro- a direct link between PDLP5 and callose synthases. Whether viding further evidence that FLS2 activity associates with lipid this link comes from direct complex formation between these rafts. This allows speculation that FLS2 activity, and ...
CHAPTER 1 PATHOGENESIS OF GOUSIEKTE 1.1
... Actin is one of the most conserved eukaryotic proteins and actin isoforms show greater than 90 % overall sequence homology, except in their 18 N-terminal residues (Lessard, 1988). The main actin in the heart is α-actin. The thin filament proteins tropomyosin (TPM) and the globular Ca2+-binding tropo ...
... Actin is one of the most conserved eukaryotic proteins and actin isoforms show greater than 90 % overall sequence homology, except in their 18 N-terminal residues (Lessard, 1988). The main actin in the heart is α-actin. The thin filament proteins tropomyosin (TPM) and the globular Ca2+-binding tropo ...
Thyroglobulin, the major and obligatory
... phosphate residues per dimeric TG. Two phosphate residues remained resistant to enzymatic treatment. They may be present as phosphoamino acids or be located on unexposed oligosaccharides. It has been shown that phosphotyrosine is the most alkaline-stable phosphoamino acid (Martensen, 1982) and that ...
... phosphate residues per dimeric TG. Two phosphate residues remained resistant to enzymatic treatment. They may be present as phosphoamino acids or be located on unexposed oligosaccharides. It has been shown that phosphotyrosine is the most alkaline-stable phosphoamino acid (Martensen, 1982) and that ...
C. Sulfonamides
... human homologue but they do not have that much similarity in structure…. Therefore trimethoprim is 1000 more active on the bacterial copy of this enzyme.. Normally used in combination with sulfamethoxazole (cotrimoxazole): ...
... human homologue but they do not have that much similarity in structure…. Therefore trimethoprim is 1000 more active on the bacterial copy of this enzyme.. Normally used in combination with sulfamethoxazole (cotrimoxazole): ...
Researchers determine how part of the endoplasmic reticulum gets
... GTPases help the tubule membranes stick together. Researchers in a different laboratory discovered another protein, lunapark, that they believe stabilizes the junctions between connected tubules, although Rapoport conducted follow-up experiments that didn't convince him. Was this handful of molecule ...
... GTPases help the tubule membranes stick together. Researchers in a different laboratory discovered another protein, lunapark, that they believe stabilizes the junctions between connected tubules, although Rapoport conducted follow-up experiments that didn't convince him. Was this handful of molecule ...
BIOLOGICAL SCIENCES - Plant Biology PP2A
... to the use of different T-DNA lines. Short root phenotypes associated with disrupted cell division and/or cellular patterning in the root tip are often associated with a loss of quiescent center cell identity (39, 40). To assess if the quiescent center was absent in the disrupted root tip of pp2a-3 ...
... to the use of different T-DNA lines. Short root phenotypes associated with disrupted cell division and/or cellular patterning in the root tip are often associated with a loss of quiescent center cell identity (39, 40). To assess if the quiescent center was absent in the disrupted root tip of pp2a-3 ...
Performance - Golden Glory Pharma Pte Ltd.
... • Target: “The Everyday Athlete”- Primary focus is 16-24 year old males who are new to sports nutrition supplements looking to improve their sports performance and/or build muscle & increase strength. Target segments include: high school athletes (4 Million+) & college athletes (400K+) ...
... • Target: “The Everyday Athlete”- Primary focus is 16-24 year old males who are new to sports nutrition supplements looking to improve their sports performance and/or build muscle & increase strength. Target segments include: high school athletes (4 Million+) & college athletes (400K+) ...
protein ppt
... coils to form a complex 3D shape • Caused by interactions between R groups (Hbonds, disulphide bridges, ionic bonds and hydrophilic / hydrophobic interactions) • Tertiary structure may be important for the function (e.g. specificity of active site in enzymes) • Globular proteins ...
... coils to form a complex 3D shape • Caused by interactions between R groups (Hbonds, disulphide bridges, ionic bonds and hydrophilic / hydrophobic interactions) • Tertiary structure may be important for the function (e.g. specificity of active site in enzymes) • Globular proteins ...
Ch 11 Slides - people.iup.edu
... • Simple but all the key features are present • Reception, Transduction, Response ...
... • Simple but all the key features are present • Reception, Transduction, Response ...
Excretory System
... THE EXCRETORY SYSTEM The excretory system helps to remove body waste safely. The kidneys act like filters. ...
... THE EXCRETORY SYSTEM The excretory system helps to remove body waste safely. The kidneys act like filters. ...
Protein phosphorylation
Protein phosphorylation is a post-translational modification of proteins in which an amino acid residue is phosphorylated by a protein kinase by the addition of a covalently bound phosphate group. Phosphorylation alters the structural conformation of a protein, causing it to become activated, deactivated, or modifying its function. The reverse reaction of phosphorylation is called dephosphorylation, and is catalyzed by protein phosphatases. Protein kinases and phosphatases work independently and in a balance to regulate the function of proteins. The amino acids most commonly phosphorylated are serine, threonine, and tyrosine in eukaryotes, and histidine in prokaryotes, which play important and well-characterized roles in signaling pathways and metabolism. However, many other amino acids can also be phosphorylated, including arginine, lysine, and cysteine. Protein phosphorylation was first reported in 1906 by Phoebus Levene at the Rockefeller Institute for Medical Research with the discovery of phosphorylated vitellin. However, it was nearly 50 years until the enzymatic phosphorylation of proteins by protein kinases was discovered.