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C2005/F2401 `07 -- Lecture 16 -- Last Edited
C2005/F2401 `07 -- Lecture 16 -- Last Edited

... 5. Cell to cell contact is required. Conjugation, unlike transformation, requires cell-cell contact and the DNA (copy) is passed across a bridge that forms temporarily between the pair of mating cells. Note that transfer is always from F+ or Hfr to F-, never the other way around or from F+ to F+, F- ...
The genomic substrate for adaptive radiation in African cichlid fish
The genomic substrate for adaptive radiation in African cichlid fish

... testis) were isolated from several individuals inbred for ∼ 60 generations in the laboratory of Dr. Hans Hoffman (University of Texas at Austin, Austin, TX, USA). Tissues for RNA were collected and placed in RNALater and blood was collected in a tube with the anticoagulant EDTA. Total RNA was extrac ...
Lecture 18
Lecture 18

... Quantitative genetics and QTL mapping - from Darwin's time onward, it has been widely recognized that natural populations harbor a considerably degree of genetic variation. - Darwin came to this conclusion from the experience of animal and plant breeders of his day and he relied on it heavily when d ...
Differentiation of plant cells during symbiotic nitrogen fixation
Differentiation of plant cells during symbiotic nitrogen fixation

... development. High-throughput methods for expression analysis are now being used to compare gene expression between symbiotic and non-symbiotic plant tissues, and thus to identify large numbers of genes that are up-regulated during nodule development. One such method relies on DNA arrays, on which th ...
pdf
pdf

... regions to consider, with 51 unique flanking genes. There are 6 super-regions with at least 99 bp overlapping with ultra-conserved elements. At least one of the flanking genes for each of these 6 super-regions is a transcription factor located 1–314 kb away (IRX3, IRX5, IRX6, HOXD13, DMRT1, DMRT3, F ...
Evolution of multicellularity and sexuality in the life cycles of
Evolution of multicellularity and sexuality in the life cycles of

... genomic basis of life cycle evolution in each lineage; and 3) the global comparative analysis across all major plant lineages in order to resolve the roles of genetic heritage, developmental mechanisms, and selection pressure in plant life cycle evolution. Of special interest is an understanding of ...
What is a gene, post-ENCODE? History and updated definition
What is a gene, post-ENCODE? History and updated definition

... History of the gene, 1860 to just before ENCODE Definition 1860s–1900s: Gene as a discrete unit of heredity The concept of the “gene” has evolved and become more complex since it was first proposed (see timeline in Fig. 1, accompanying poster). There are various definitions of the term, although com ...
Divergent Evolution and Evolution by the Birth-and
Divergent Evolution and Evolution by the Birth-and

... The total number of Vu and outgroup genes used for our study of long-term evolution was 57, and they are listed in table 1. We used the germ-line sequences whenever possible, to avoid the effect of somatic mutation ( Tonegawa 1983 ) . In a few species, however, we used cDNA, because germ-line genes ...
meiosis_9_for_VLE
meiosis_9_for_VLE

The dawn of plant salt tolerance genetics
The dawn of plant salt tolerance genetics

... stresses. Soon, lists of stress tolerance determinants, both effectors and signal molecules, will be compiled on the basis of functional rather than correlative evidence. The genetics approach and application of genomics concepts is bringing the physiology of salt tolerance back from a cellular to a ...
and genomics - FISH-BOL
and genomics - FISH-BOL

... • Led by Michael Rudnicki @ Ottawa Health Research Institute • map the mechanisms and networks that control the genes that comprise the genome • $$$ to be determined …. ultimately, $50M - $100M; program development funds from MEDT • potential ICI: Canada, Australia, France, Germany, Italy, Netherlan ...
Slide 1
Slide 1

... Traits observed and exhibited by an individual are referred to as phenotypes. The coat color exhibited by a dog for instance would be the phenotype. The actual combination of genes that produce the phenotype is called the genotype. Gregor Mendel has shown us that traits are controlled by pairs of g ...
Signals of recent positive selection in a worldwide sample of human
Signals of recent positive selection in a worldwide sample of human

... surrounding each of 10,000 random SNPs. The dotted lines shows the position beyond which 5% of the random SNPs fall, and the solid lines the position beyond which 1% of the random SNPs fall. Gene names that are starred fall in the 5% tail of at least one comparison, and those with two stars fall in ...
embj201490542-sup-0013
embj201490542-sup-0013

... target genes. To define genes that become induced upon Scl expression, SclhCD4 reporter ES cells (Chung et al, 2002) were used to identify genes that become up-regulated in day 4 Scl-expressing mesoderm (Flk1+Scl+) as compared to Flk1+Scl- mesodermal precursors that give rise to other mesodermal lin ...
Sex-Linked Genes - Doctor Jade Main
Sex-Linked Genes - Doctor Jade Main

... from each parent for each trait • specific trait may not show up in an individual • may be passed to next generation • from his results, Mendel described four specific hypotheses ...
File
File

... • conjugative or non-conjugative – depending upon whether or not they carry a set of transfer genes, called the tra genes, which promote bacterial conjugation • On the basis of their being maintained as multiple copies per cell (relaxed plasmids) or as a limited number of copies per cell (stringent ...
"What is a gene, in the post-ENCODE era?"
"What is a gene, in the post-ENCODE era?"

... History of the gene, 1860 to just before ENCODE Definition 1860s–1900s: Gene as a discrete unit of heredity The concept of the “gene” has evolved and become more complex since it was first proposed (see timeline in Fig. 1, accompanying poster). There are various definitions of the term, although com ...
Genetic Control of Cell Function and Inheritance
Genetic Control of Cell Function and Inheritance

... Proteins are made from a standard set of amino acids, which are joined end to end to form the long polypeptide chains of protein molecules. Each polypeptide chain may have as many as 100 to more than 300 amino acids in it. The process of protein synthesis is called translation because the genetic co ...
bioinformatics review - e
bioinformatics review - e

... standard for the secondary analysis of high throughput experiments and a large number of tools have been developed for this purpose. We present a detailed comparison of 14 such tools using the following criteria: scope of the analysis, visualization capabilities, statistical model(s) used, correctio ...
10-2 & 11-2 Mendel Genetics 2010
10-2 & 11-2 Mendel Genetics 2010

... Exceptions to Simple Dominance Does the segregation of one pair of alleles affect the segregation of another pair of alleles? • For example, does the gene that determines whether a seed is round or wrinkled in shape have anything to do with the gene for seed color? Must a round seed also be yellow? ...
Cladistic analyses of molecular characters
Cladistic analyses of molecular characters

... features. Ribosomal RNA ...
Original 2013 answers page as a complete
Original 2013 answers page as a complete

... “I will use the definition that the changes are assumed to be random with respect to physiological function and could not therefore be influenced by such function or by functional changes in response to the environment. This is the assumption that excludes the phenotype from in any way influencing o ...
Normalization of gene expression measurements in tumor tissues
Normalization of gene expression measurements in tumor tissues

... two or three candidate genes were compared.3,4,9–11 In the present study, we attempted to identify a single housekeeping gene that could replace the measurement of multiple genes. Therefore, expression patterns of 13 ordinarily used housekeeping genes were investigated in 80 epithelial tissue sample ...
Types of plasmid One way of grouping plasmids is by their ability to
Types of plasmid One way of grouping plasmids is by their ability to

... harbors them. The plasmids are next inserted into bacteria by a process called transformation, which are then grown on specific antibiotic(s). Bacteria which took up one or more copies of the plasmid then express (make protein from) the gene that confers antibiotic resistance. This is typically a pr ...
Towards safer vectors for the field release of recombinant bacteria
Towards safer vectors for the field release of recombinant bacteria

... the case of most Gram-negative bacteria, broad hostrange plasmid vectors (such as RSF1010 or RK2), able to be mobilized and to replicate in most kinds of Gramnegative bacteria, are usually used; further complicating the problem of horizontal transfer to other species (Davison, 1999; Davison, 2002). ...
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Minimal genome

The concept of minimal genome assumes that genomes can be reduced to a bare minimum, given that they contain many non-essential genes of limited or situational importance to the organism. Therefore, if a collection of all the essential genes were put together, a minimum genome could be created artificially in a stable environment. By adding more genes, the creation of an organism of desired properties is possible. The concept of minimal genome arose from the observations that many genes do not appear to be necessary for survival. In order to create a new organism a scientist must determine the minimal set of genes required for metabolism and replication. This can be achieved by experimental and computational analysis of the biochemical pathways needed to carry out basic metabolism and reproduction. A good model for a minimal genome is Mycoplasma genitalium, the organism with the smallest known genome. Most genes that are used by this organism are usually considered essential for survival; based on this concept a minimal set of 256 genes has been proposed.
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