Lluís Millán Ariño GENOMIC DISTRIBUTION AND FUNCTIONAL SPECIFICITY OF
... 3.1. All H1 variants are non-specifically present at gene promoters and are depleted from transcription start sites (TSS) in active genes In our first attempt to elucidate H1 variant distribution in the genome, we tested occupancy in specific loci corresponding to diverse genomic features. Therefore ...
... 3.1. All H1 variants are non-specifically present at gene promoters and are depleted from transcription start sites (TSS) in active genes In our first attempt to elucidate H1 variant distribution in the genome, we tested occupancy in specific loci corresponding to diverse genomic features. Therefore ...
Simulating evolution by gene duplication of protein features that
... figure) in the gene coding for the protein before a null mutation occurs elsewhere in the gene (indicated by a red “X”), then several amino acid residues will have been altered and the new selectable MR feature will have been successfully built in the protein (indicated by the greenshaded area). By ...
... figure) in the gene coding for the protein before a null mutation occurs elsewhere in the gene (indicated by a red “X”), then several amino acid residues will have been altered and the new selectable MR feature will have been successfully built in the protein (indicated by the greenshaded area). By ...
3.3 How Do You Identify and Clone a Gene of Interest?
... – Developed in the 1980s by Kary Mullis – Technique for making copies, or amplifying, a specific sequence of DNA in a short period of time – Process • Target DNA to be amplified is added to a tube, mixed with nucleotides (dATP, dCTP, dGTP, dTTP), buffer, and DNA polymerase. • Primers are added – sho ...
... – Developed in the 1980s by Kary Mullis – Technique for making copies, or amplifying, a specific sequence of DNA in a short period of time – Process • Target DNA to be amplified is added to a tube, mixed with nucleotides (dATP, dCTP, dGTP, dTTP), buffer, and DNA polymerase. • Primers are added – sho ...
Arabidopsis AtCAP-C Disruption of the SMC4 gene,
... opening of the first flower bud. The entire inflorescence was then fixed in 3:1 ethanol and acetic acid. Fixation was done for 24–36 h, with the fixative being removed and replaced after 3 h. After fixation the buds were rinsed three times in 70% ethanol, and then either used immediately or stored at 4C ...
... opening of the first flower bud. The entire inflorescence was then fixed in 3:1 ethanol and acetic acid. Fixation was done for 24–36 h, with the fixative being removed and replaced after 3 h. After fixation the buds were rinsed three times in 70% ethanol, and then either used immediately or stored at 4C ...
Genomic gains and losses influence expression levels of genes
... as compared to all other subtypes, were equally distributed over the genome in AML subgroups with trisomies. In contrast, 30 and 86% of the most differentially expressed genes characteristic for AML with 5q deletion and monosomy 7 are located on chromosomes 5 or 7. In conclusion, gain of whole chrom ...
... as compared to all other subtypes, were equally distributed over the genome in AML subgroups with trisomies. In contrast, 30 and 86% of the most differentially expressed genes characteristic for AML with 5q deletion and monosomy 7 are located on chromosomes 5 or 7. In conclusion, gain of whole chrom ...
Primer on Molecular Genetics
... about 10% of the genome is known to include the protein-coding sequences (exons) of genes. Interspersed within many genes are intron sequences, which have no coding function. The balance of the genome is thought to consist of other noncoding regions (such as control sequences and intergenic regions) ...
... about 10% of the genome is known to include the protein-coding sequences (exons) of genes. Interspersed within many genes are intron sequences, which have no coding function. The balance of the genome is thought to consist of other noncoding regions (such as control sequences and intergenic regions) ...
Chapter 18: REGULATION OF GENE EXPRESSION
... as a single unit (encoding proteins of the same metabolic pathway). A regulator gene, located outside the operon, codes for a repressor that controls whether the operon is active or not. An operon includes the following elements: Promoter—A short sequence of DNA where RNA polymerase first attach ...
... as a single unit (encoding proteins of the same metabolic pathway). A regulator gene, located outside the operon, codes for a repressor that controls whether the operon is active or not. An operon includes the following elements: Promoter—A short sequence of DNA where RNA polymerase first attach ...
Evaluation of Nyholt`s Procedure for Multiple Testing Correction
... very strong LD. Our empirical results, on the other hand, show that it tends to become anti-conservative. The website of the SNPSpD program contains a note describing a way of overcoming this. The suggestion is to exclude all SNPs but one from any set of SNPs that are in perfect LD. This is, however ...
... very strong LD. Our empirical results, on the other hand, show that it tends to become anti-conservative. The website of the SNPSpD program contains a note describing a way of overcoming this. The suggestion is to exclude all SNPs but one from any set of SNPs that are in perfect LD. This is, however ...
Zeeberg - Gene Ontology Consortium
... • GoMiner traditionally dereplicates input files so that only one instance of a gene name is processed • When multiple alternatively spliced forms are to be analyzed, however, dereplication would result in a loss of relevant information • Consequently, we have added a new feature to GoMiner to retai ...
... • GoMiner traditionally dereplicates input files so that only one instance of a gene name is processed • When multiple alternatively spliced forms are to be analyzed, however, dereplication would result in a loss of relevant information • Consequently, we have added a new feature to GoMiner to retai ...
We need an optimality criterion to choose a best estimate (tree
... organisms are so different? There are not likely to be many sequence homologies between bacteria, archaea, and eukaroytes. ...
... organisms are so different? There are not likely to be many sequence homologies between bacteria, archaea, and eukaroytes. ...
An Empirical Test for Branch-Specific Positive Selection
... and exons were assembled to form the entire coding sequence of the gene. The resulting alignments were reformatted to serve as input to the phylogenetic analysis programs. The total sequence from each source (generated for this study and previously publicly available) and the average gene coverage f ...
... and exons were assembled to form the entire coding sequence of the gene. The resulting alignments were reformatted to serve as input to the phylogenetic analysis programs. The total sequence from each source (generated for this study and previously publicly available) and the average gene coverage f ...
File
... Stressful environmental conditions may cause some bacteria to increase mutation rates. This can actually be helpful to the organism, since mutations may sometimes give such bacteria new traits, such as the ability to consume a new food source or to resist a poison in the environment. ...
... Stressful environmental conditions may cause some bacteria to increase mutation rates. This can actually be helpful to the organism, since mutations may sometimes give such bacteria new traits, such as the ability to consume a new food source or to resist a poison in the environment. ...
Appendix S2.
... Most published trees contained only a few taxa of interest for any particular group. Therefore, we were often forced to use multiple trees which may have employed different characters and methods in their analyses to place particular taxa into our tree (e.g. Elapideae, see below). ...
... Most published trees contained only a few taxa of interest for any particular group. Therefore, we were often forced to use multiple trees which may have employed different characters and methods in their analyses to place particular taxa into our tree (e.g. Elapideae, see below). ...
Ewens` sampling formula and related formulae: combinatorial proofs
... Label the sample genes and list them in the order in which they are lost backward in time, following either a mutation or a coalescence. In the case of coalescence, one of the two genes involved is chosen at random to continue back in time as a parent gene and the other gene is lost. There are n! di ...
... Label the sample genes and list them in the order in which they are lost backward in time, following either a mutation or a coalescence. In the case of coalescence, one of the two genes involved is chosen at random to continue back in time as a parent gene and the other gene is lost. There are n! di ...
Social implications of gene therapy
... it is unprecedented and technologically sophisticated, and because it permits alteration of something considered fundamental to each individual— his or her genetic constitution. While genetic changes have been technologically induced for years—for example, in the use of some vaccines-the changes hav ...
... it is unprecedented and technologically sophisticated, and because it permits alteration of something considered fundamental to each individual— his or her genetic constitution. While genetic changes have been technologically induced for years—for example, in the use of some vaccines-the changes hav ...
slides
... Micro-arrays are still quite costly per array (but not per gene). Large data sets have about 100 arrays data sets with only a few arrays are very common Study designs depend on the field of application (plants/animals/human). In non-human applications material is often pooled to reduce the number of ...
... Micro-arrays are still quite costly per array (but not per gene). Large data sets have about 100 arrays data sets with only a few arrays are very common Study designs depend on the field of application (plants/animals/human). In non-human applications material is often pooled to reduce the number of ...
Gene Section NUP98 (nucleoporin 98 kDa) Atlas of Genetics and Cytogenetics
... NUP98-RAP1GDS1 Disease 3 cases of adult T-ALL. Hybrid/Mutated gene 5' NUP98 - 3' RAP1GDS1. Abnormal protein Fuses the GLFG repeat domains of NUP98 to the entire coding region of RAP1GDS1. The product, rap1gds, has guanine nucleotide exchange factor activity. ...
... NUP98-RAP1GDS1 Disease 3 cases of adult T-ALL. Hybrid/Mutated gene 5' NUP98 - 3' RAP1GDS1. Abnormal protein Fuses the GLFG repeat domains of NUP98 to the entire coding region of RAP1GDS1. The product, rap1gds, has guanine nucleotide exchange factor activity. ...
CRISPR-Cas9 Mouse Toolbox
... enable the rapid ligation of annealed and phosphorylated oligos designed based on the target site sequence (20bp). The cloning protocol can be found below. ...
... enable the rapid ligation of annealed and phosphorylated oligos designed based on the target site sequence (20bp). The cloning protocol can be found below. ...
Chapter 11 and 12 from Campbell Biology 10th Edition By Keshara
... phenotypic character (coat color) they follow the law of independent assortment represents an F1 dithered cross as result of epistatis the phenotypic ratio of G2 offspring is 9:3:4 and other epistatis produce different rations but are all modified versions of 9:3:3:1 Polygenic inheritance fo ...
... phenotypic character (coat color) they follow the law of independent assortment represents an F1 dithered cross as result of epistatis the phenotypic ratio of G2 offspring is 9:3:4 and other epistatis produce different rations but are all modified versions of 9:3:3:1 Polygenic inheritance fo ...
FURTHER CONSIDERATION OF A MOUSE MODEL MALIGNANT PHEOCHROMOCYTOMA FOR Review Article
... model, are responsible for the suppression of metastases. Thus, by screening them with macroarrays, we verified the suppression of ...
... model, are responsible for the suppression of metastases. Thus, by screening them with macroarrays, we verified the suppression of ...
Silencing by nuclear matrix attachment distinguishes cell
... Illumina Bead Studio software suite. For each reporter, the average signal was q-spline normalized and expressed genes were identified by signal values higher than internal spike-in controls for expression (Smax>3000). Ontological analysis was carried out using either Panther (27) or David (http://da ...
... Illumina Bead Studio software suite. For each reporter, the average signal was q-spline normalized and expressed genes were identified by signal values higher than internal spike-in controls for expression (Smax>3000). Ontological analysis was carried out using either Panther (27) or David (http://da ...
Two-way clustering
... Oligonucleotide sequences (oligos) probes: 25 nucleotide chains for selected parts of a gene complementary to mRNA. For every gene there are 1120(depending on chip design) of different oligo probes called perfect matches (PM). In addition, there are mismatch oligos (MM) corresponding to each of the ...
... Oligonucleotide sequences (oligos) probes: 25 nucleotide chains for selected parts of a gene complementary to mRNA. For every gene there are 1120(depending on chip design) of different oligo probes called perfect matches (PM). In addition, there are mismatch oligos (MM) corresponding to each of the ...
How functions are combined within the ABC model
... carp Formation of multimeric complexes suggests mechanism for combining A/B and B/C functions within the ABC model. ...
... carp Formation of multimeric complexes suggests mechanism for combining A/B and B/C functions within the ABC model. ...
Genome evolution
Genome evolution is the process by which a genome changes in structure (sequence) or size over time. The study of genome evolution involves multiple fields such as structural analysis of the genome, the study of genomic parasites, gene and ancient genome duplications, polyploidy, and comparative genomics. Genome evolution is a constantly changing and evolving field due to the steadily growing number of sequenced genomes, both prokaryotic and eukaryotic, available to the scientific community and the public at large.