Lactic acid bacteria as a cell factory: rerouting of carbon metabolism
... metabolism. Proper appreciation of the application potential of LAB as cell factories is related to several features that are shared by these micro-organisms. In general they have a long history of safe use and grow rapidly on media that are derived from milk and contain lactose as the major carbon ...
... metabolism. Proper appreciation of the application potential of LAB as cell factories is related to several features that are shared by these micro-organisms. In general they have a long history of safe use and grow rapidly on media that are derived from milk and contain lactose as the major carbon ...
Identifying a Novel Isoform of the AZIN1 Gene by Combining High
... separation of PBMCs from whole blood (2) extraction of RNA from PBMCs (3) creation of cDNA libraries (4) whole genome sequencing with Illumina RNA-seq (5) identification of novel isoform with PacBio technology (6) validation with Sanger sequencing. PacBio sequencing revealed a new isoform of the AZI ...
... separation of PBMCs from whole blood (2) extraction of RNA from PBMCs (3) creation of cDNA libraries (4) whole genome sequencing with Illumina RNA-seq (5) identification of novel isoform with PacBio technology (6) validation with Sanger sequencing. PacBio sequencing revealed a new isoform of the AZI ...
Prioritizing curation of mouse genes by paucity of annotations and
... diseases including some types of retinitis pigmentosa and polycystic kidney disease. Once thought to be restricted to a few cell types, it is now clear that primary cilia are found on almost all vertebrate cells and are critical to Sonic hedgehog (Shh) signaling. Mouse models play a key role in deve ...
... diseases including some types of retinitis pigmentosa and polycystic kidney disease. Once thought to be restricted to a few cell types, it is now clear that primary cilia are found on almost all vertebrate cells and are critical to Sonic hedgehog (Shh) signaling. Mouse models play a key role in deve ...
13-2 - Lincoln Park High School
... “Synthetic” sequences can be joined to “natural” sequences using enzymes that splice DNA together. ...
... “Synthetic” sequences can be joined to “natural” sequences using enzymes that splice DNA together. ...
evolution - Janelia Research Campus
... the gene exists in every cell of the body, it may be expected to affect the organism as a whole, even if its most striking effect is on some particular organ or function. Thus the gene Ch in Primula sinensis incises the petals, doubles the number of sepals, breaks up the bracts, produces a more comp ...
... the gene exists in every cell of the body, it may be expected to affect the organism as a whole, even if its most striking effect is on some particular organ or function. Thus the gene Ch in Primula sinensis incises the petals, doubles the number of sepals, breaks up the bracts, produces a more comp ...
12864_2008_1659_MOESM3_ESM
... sequence similarity, we assume that YWHAB spawned the retrogene YWHAH. The latter covers all of the exons (no corresponding introns) of the parent gene and has been preserved from fish to mammals. After the retroposition event, YWHAG probably was derived from YWHAH, or vice versa, by segmental dupli ...
... sequence similarity, we assume that YWHAB spawned the retrogene YWHAH. The latter covers all of the exons (no corresponding introns) of the parent gene and has been preserved from fish to mammals. After the retroposition event, YWHAG probably was derived from YWHAH, or vice versa, by segmental dupli ...
1 Engineering Lipases with an Expanded Genetic Code - Wiley-VCH
... substrates, for example, usage of the other enantiomer of a racemic mixture as substrate [2]. Among commonly used strategies, rational protein design and directed evolution are increasingly applied in a synergistic manner. Directed evolution has been used as a preferred method to alter the propertie ...
... substrates, for example, usage of the other enantiomer of a racemic mixture as substrate [2]. Among commonly used strategies, rational protein design and directed evolution are increasingly applied in a synergistic manner. Directed evolution has been used as a preferred method to alter the propertie ...
Identification of Vietnamese Coptotermes pest species based on the
... from Van Quan and Tan Linh. The mismatches may reduce the PCR efficiency, and perhaps the amplification products could be obtained by chance at the condition we employed in this study. Therefore, for the identification of closely related Coptotermes species by 16S rRNA gene, determination of nucleot ...
... from Van Quan and Tan Linh. The mismatches may reduce the PCR efficiency, and perhaps the amplification products could be obtained by chance at the condition we employed in this study. Therefore, for the identification of closely related Coptotermes species by 16S rRNA gene, determination of nucleot ...
13-2 Manipulating DNA
... “Synthetic” sequences can be joined to “natural” sequences using enzymes that splice DNA together. ...
... “Synthetic” sequences can be joined to “natural” sequences using enzymes that splice DNA together. ...
Challenges in identifying genetic risk factors for common
... been spent to identify DNA variants in human genome that are more common in patients with a specific complex disease than in healthy individuals, the search for identification of major risk factors for complex disorders have remained elusive1. Success is visible recently in exceptional situations li ...
... been spent to identify DNA variants in human genome that are more common in patients with a specific complex disease than in healthy individuals, the search for identification of major risk factors for complex disorders have remained elusive1. Success is visible recently in exceptional situations li ...
word - Institute of Public Health
... The aim of the project is to develop and evaluate an electronic data entry tool that enables parents to communicate data essential to treatment of their children, regardless of their own literacy skills. This work addresses two health information technology goals of the Department of Health and Huma ...
... The aim of the project is to develop and evaluate an electronic data entry tool that enables parents to communicate data essential to treatment of their children, regardless of their own literacy skills. This work addresses two health information technology goals of the Department of Health and Huma ...
1 Micro TA Questions
... Group A Strep demonstrate significant resistance to phagoctytic killing when placed in fresh human blood. This resistance can be most effectively overcome by adding antibodies to which of the following? A) B) C) D) E) ...
... Group A Strep demonstrate significant resistance to phagoctytic killing when placed in fresh human blood. This resistance can be most effectively overcome by adding antibodies to which of the following? A) B) C) D) E) ...
Points to take note for Biology - Learning Made Simple Singapore
... - Enzymes are biological catalysts made of proteins which speed up rate of chemical reactions and are chemically unchanged at end of reactions. - Take note of lock-and-key hypothesis. - Enzyme is lock. Substrate is key. - Enzyme action is specific as each enzyme only allows a particular substrate to ...
... - Enzymes are biological catalysts made of proteins which speed up rate of chemical reactions and are chemically unchanged at end of reactions. - Take note of lock-and-key hypothesis. - Enzyme is lock. Substrate is key. - Enzyme action is specific as each enzyme only allows a particular substrate to ...
MODELING GENE EXPRESSION FROM MICROARRAY
... data, and capable of reducing the complexity of the data to make it comprehensible. Substantial effort is being made to build models to analyze it. Non-hierarchical clustering techniques such as k-means clustering are a class of mixture model-based approaches4. They group genes with similar expressi ...
... data, and capable of reducing the complexity of the data to make it comprehensible. Substantial effort is being made to build models to analyze it. Non-hierarchical clustering techniques such as k-means clustering are a class of mixture model-based approaches4. They group genes with similar expressi ...
Altered adherence properties of a Streptococcus
... chloramphenicol. The recombinant pMHlO9 was then digested with BamHI to release a 1.3 kb cshA promoter (p&A)-cat fragment which was cloned into BamHI-cut pSF143 (Tao et al., 1992) to generate plasmid pSFCAT1. Plasmid pSFCATl was introduced onto the S. gordonii chromosome by transformation and select ...
... chloramphenicol. The recombinant pMHlO9 was then digested with BamHI to release a 1.3 kb cshA promoter (p&A)-cat fragment which was cloned into BamHI-cut pSF143 (Tao et al., 1992) to generate plasmid pSFCAT1. Plasmid pSFCATl was introduced onto the S. gordonii chromosome by transformation and select ...
Missense mutations in the PAX6 gene in aniridia.
... were detected after careful analysis of PCR products from patients' genomic DNA. Patient 1 had two nucleotide substitutions and insertion of a short oligonucleotide on the same allele; nucleotide substitutions from A to G occurred at the 467th and 502nd positions of its cDNA form (in this study, the ...
... were detected after careful analysis of PCR products from patients' genomic DNA. Patient 1 had two nucleotide substitutions and insertion of a short oligonucleotide on the same allele; nucleotide substitutions from A to G occurred at the 467th and 502nd positions of its cDNA form (in this study, the ...
Biol 1406 notes Ch 15 8thed
... Morgan reasoned that body color and wing shape are usually inherited together because the genes for these characters are on the same chromosome. The other two phenotypes (gray-vestigial and black-normal) were rarer than expected based on independent assortment (but totally unexpected from depend ...
... Morgan reasoned that body color and wing shape are usually inherited together because the genes for these characters are on the same chromosome. The other two phenotypes (gray-vestigial and black-normal) were rarer than expected based on independent assortment (but totally unexpected from depend ...
Use of wheat gene resources with different grain colour in breeding
... and snow mould, and frequent drying of grain. Lines with purple pericarp often exhibit small grains, whereas their resistance to FHB is usually good. Back-crosses are used for the transmission of non-traditional colour into the genetic background of common wheat varieties. It will be important to un ...
... and snow mould, and frequent drying of grain. Lines with purple pericarp often exhibit small grains, whereas their resistance to FHB is usually good. Back-crosses are used for the transmission of non-traditional colour into the genetic background of common wheat varieties. It will be important to un ...
Different Species Common Arthritis Quantitative Trait Loci in High
... SNPs have been identified (11). Therefore, polymorphisms within a QTL identified in those 16 strains or their substrains can be obtained, which will considerably help in the identification of candidate genes. Previously we performed a genome-wide linkage analysis in a F2 progeny to identify QTLs con ...
... SNPs have been identified (11). Therefore, polymorphisms within a QTL identified in those 16 strains or their substrains can be obtained, which will considerably help in the identification of candidate genes. Previously we performed a genome-wide linkage analysis in a F2 progeny to identify QTLs con ...
Gregor Mendels Experiments and Outcome dominnat and recessive
... When sex cells (sperm and egg) are being made, it is like the toss of a coin for which allele will go into the sex cell. It’s a 50/50 chance. But remember, a parent can only give a variation of an allele that they have in their genotypeYOU CAN’T GIVE SOMETHING YOU DO NOT HAVE ...
... When sex cells (sperm and egg) are being made, it is like the toss of a coin for which allele will go into the sex cell. It’s a 50/50 chance. But remember, a parent can only give a variation of an allele that they have in their genotypeYOU CAN’T GIVE SOMETHING YOU DO NOT HAVE ...
Low Levels of Nucleotide Diversity at Homoeologous Adh Loci in
... and other studies have yielded a number of insights into the factors that shape naturally occurring variation, in any particular case, the evolutionary or historical forces responsible for the diversity patterns observed may be difficult to discern. This is especially true for comparisons between sp ...
... and other studies have yielded a number of insights into the factors that shape naturally occurring variation, in any particular case, the evolutionary or historical forces responsible for the diversity patterns observed may be difficult to discern. This is especially true for comparisons between sp ...
Identification of the target DNA sequence and characterization of
... HlyU, a transcriptional regulator common in many Vibrio species, activates the hemolysin gene hlyA in Vibrio cholerae, the rtxA1 operon in Vibrio vulnificus and the genes of plp-vah1 and rtxACHBDE gene clusters in Vibrio anguillarum. The protein is also proposed to be a potential global virulence re ...
... HlyU, a transcriptional regulator common in many Vibrio species, activates the hemolysin gene hlyA in Vibrio cholerae, the rtxA1 operon in Vibrio vulnificus and the genes of plp-vah1 and rtxACHBDE gene clusters in Vibrio anguillarum. The protein is also proposed to be a potential global virulence re ...
QTL-mapping of individual resistance against American Apis mellifera
... (Qiagen, Cat. No.69506). DNA concentrations were measured using the Nano Drop ND 1000 Spectrophotometer (v 3.5.2) and equal amounts of DNA per individual were pooled according to the defined susceptible (n = 35) and resistant (n = 121) phenotypes. In this step a total of 573 fluorescent tagged micro ...
... (Qiagen, Cat. No.69506). DNA concentrations were measured using the Nano Drop ND 1000 Spectrophotometer (v 3.5.2) and equal amounts of DNA per individual were pooled according to the defined susceptible (n = 35) and resistant (n = 121) phenotypes. In this step a total of 573 fluorescent tagged micro ...
BBSI Final Report
... Before capsid size difference is confirmed, however, the area will be divided up further to narrow down the gene or genes responsible for capsid size difference between transducing and phage particles. These smaller regions will be cloned into pPV72 as well and interference tested through spot infec ...
... Before capsid size difference is confirmed, however, the area will be divided up further to narrow down the gene or genes responsible for capsid size difference between transducing and phage particles. These smaller regions will be cloned into pPV72 as well and interference tested through spot infec ...
Genetic engineering
Genetic engineering, also called genetic modification, is the direct manipulation of an organism's genome using biotechnology. It is therefore a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms. New DNA may be inserted in the host genome by first isolating and copying the genetic material of interest using molecular cloning methods to generate a DNA sequence, or by synthesizing the DNA, and then inserting this construct into the host organism. Genes may be removed, or ""knocked out"", using a nuclease. Gene targeting is a different technique that uses homologous recombination to change an endogenous gene, and can be used to delete a gene, remove exons, add a gene, or introduce point mutations.An organism that is generated through genetic engineering is considered to be a genetically modified organism (GMO). The first GMOs were bacteria generated in 1973 and GM mice in 1974. Insulin-producing bacteria were commercialized in 1982 and genetically modified food has been sold since 1994. Glofish, the first GMO designed as a pet, was first sold in the United States December in 2003.Genetic engineering techniques have been applied in numerous fields including research, agriculture, industrial biotechnology, and medicine. Enzymes used in laundry detergent and medicines such as insulin and human growth hormone are now manufactured in GM cells, experimental GM cell lines and GM animals such as mice or zebrafish are being used for research purposes, and genetically modified crops have been commercialized.