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annotation and analysis of newly discovered mycobacteriophage
annotation and analysis of newly discovered mycobacteriophage

... protein encoding capacity of the Dori genome. We identified open reading frames in both positive and negative strands of the genome and annotated a total of 93 proteincoding genes in phage Dori. BLAST was used to determine whether Dori was similar to any other bacteriophage currently sequenced and w ...
Bio 111 Handout for Genetics 1 Bio 111 iClicker Question #1
Bio 111 Handout for Genetics 1 Bio 111 iClicker Question #1

... In lecture, I talked about “counting unrelated carriers” - finding out how many people had to bring in a disease allele to explain a particular pedigree. This was useful in the case where more than one mode of inheritance was possible but you were asked to determine which was more likely. There are ...
FEMS Microbiology Ecology
FEMS Microbiology Ecology

... similarly distributed throughout the di¡erent genera isolated, which were Pseudomonas, Bacillus, Xanthomonas, Agrobacterium, Actinomyces, and Acinetobacter. Recently, 28 bacterial genera a¤liated with the phyla Proteobacteria, Firmicutes and Flexibacter/Cytophaga/Bacteroides were isolated from 640 p ...
REDESIGN OF CARNITINE ACETYLTRANSFERASE SPECIFICITY BY PROTEIN ENGINEERING UNIVERSIDAD DE BARCELONA
REDESIGN OF CARNITINE ACETYLTRANSFERASE SPECIFICITY BY PROTEIN ENGINEERING UNIVERSIDAD DE BARCELONA

... A S. cerevisiae strain (FY23∆cat2) devoid of COT and CPT activity and lacking the endogenous CAT2 gene was used as an expression system (Swiegers, 2001). Although this strain conserves two additional CrAT genes (YAT1 and YAT2), its carnitine acetyltransferase activity in the conditions in which it w ...
Inferring gene-to-phenotype and gene-to
Inferring gene-to-phenotype and gene-to

... reporters), need to be computationally excluded from consideration. For example, mice carrying an inducible knock-in of a mutant form of mouse Kcnj11 in the Gt(ROSA)26Sor locus and a transgene expressing cre recombinase in pancreatic cells, Tg(Ins2-cre)23Herr (genotype MGI:4430413), are annotated to ...
milova_032405_glass
milova_032405_glass

...  Gene Ontology annotation for all GO IDs is kept in three different information fields: biological processes, molecular function and cellular compartment. For each of the fields all available annotation was prefiltered with redundancy check and concatenated. Kate Milova ...
Gene Section
Gene Section

... EVI1 has 1051 amino acids and is a 145-kDa DNA binding protein. It contains two domains of seven and three sets of repeats of the zinc finger motif, a repression domain between the two sets of zinc fingers, and an acidic domain at the C-terminal end. Predicted translation of MDS1-EVI1 adds 188 amino ...
pdf
pdf

... thought to be incompatible with their metabolism. With the advent of epifluorescence microscopic enumeration methods in the mid 1970s, it was recognized that most of the cells present in aquatic and soil systems cannot be cultivated, although it is not known if this applies equally well to all bacte ...
Current Second Tier and Future Applications of Gene Sequencing in
Current Second Tier and Future Applications of Gene Sequencing in

... c.235G>A (p.A79T); c.246C>G (p.H82Q); c.755G>A (p.D223N); c.965T>A (p.V322E); • 3 Black infants are p.A79T / p.A79T [MAF=2.8%; Blacks only] • 2 Black infants are p.A79T / p.V322E [MAF<1% Blacks & Eur. Am.] • 1 Black infant is p.A79T / p.D223N [MAF <1%; Blacks only] • p.H82Q (MAF<1% in both European ...
Structural analysis of both products of a reciprocal translocation
Structural analysis of both products of a reciprocal translocation

... Comparative r e s t r i c t i o n mapping and b l o t hybridization experiments showed that this fragment contains Immunoglobulin heavy chain sequences extending from J5 to the 5' ...
p53 AND CANCER - Journal of Chemical and Pharmaceutical
p53 AND CANCER - Journal of Chemical and Pharmaceutical

... Trp53 is a master transcription factor which regulates the expression of a plethora of genes involved in the crucial biological processes, many of which encipher proteins that control the cell cycle or induce apoptosis. Because of its critical impact on cell predestination, cellular p53 activity mus ...
University of Bucharest, Faculty of Biology, Molecular Biology Center
University of Bucharest, Faculty of Biology, Molecular Biology Center

... heterodimeric integrin. Integrins are adhesion molecules that mediate the entry and passage of neutrophiles across membranes to destroy invading pathogens [6; 9; 10]. The molecular basis of BLAD is a single point mutation (A-G) at position 383 in the cDNA of the CD18 gene. This mutation results in a ...
Homology - a persona..
Homology - a persona..

... in Fig. 1), should be used whenever that relation is known or assumed, and the term homolog should be reserved for those cases where (a) homology can be inferred but not the subtype, or (b) the assertion is correct for all subtypes. If there is more than one ortholog, which one is ‘correct’? There i ...
Sequence analysis of three mitochondrial DNA molecules reveals
Sequence analysis of three mitochondrial DNA molecules reveals

... (including S.cerevisiae) and Saccharomyces sensu lato yeasts (including Saccharomyces castellii and Saccharomyces servazzii). In addition, C.glabrata is very closely related to the sensu lato group (20). In the sensu stricto group the mtDNA sizes ranges from 64 to 86 kb, whereas the sensu lato group ...
Chapter 13 Meiosis - Trimble County Schools
Chapter 13 Meiosis - Trimble County Schools

... Inheritance of Genes • Genes are the units of heredity, and are made up of segments of DNA • Genes are passed to the next generation via reproductive cells called gametes (sperm and eggs) • Each gene has a specific location called a locus on a certain chromosome • Most DNA is packaged into chromoso ...
Floral Symmetry - Coen Lab
Floral Symmetry - Coen Lab

... and carpels (brown). The dorsoventral axis illustrates the dorsal (blue), lateral (light brown) and ventral (pale yellow) organ types. Note that the dorsal stamen (circle) is arrested at an early stage in development. ...
Recombination
Recombination

... past. Without recombination, in a strictly asexual population, every gene shares the same ancestor. In sexually reproducing populations, however, gene genealogies will differ, as they are being shuffled by recombination. This can be well illustrated with parts of the human genome differing in their ...
15_detaillectout
15_detaillectout

... homologous chromosomes at metaphase I of meiosis, which leads to the independent assortment of alleles. ...
Roles of BRCA1 and BRCA2 in homologous recombination, DNA
Roles of BRCA1 and BRCA2 in homologous recombination, DNA

... The genetic basis for familial breast cancer predisposition has become established over the past decade with the cloning of two major breast cancer susceptibility genes, BRCA1 and BRCA2 (Miki et al., 1994; Wooster et al., 1995). The BRCA1 gene was first cloned in 1994 after being mapped to chromosome ...
Lesson Overview - Enfield High School
Lesson Overview - Enfield High School

... Genes and the Environment Example = Western white butterfly Western white butterflies that hatch in the summer have different color patterns on their wings than those hatching in the spring  Those hatching in springtime had greater levels of pigment in their wings than those hatching in the summer ...
Invitation Letter
Invitation Letter

... upcoming Semmelweis Symposium to be held in Budapest between 3-4th of November, 2005. The title of the symposium is: Semmelweis Symposium: Inflammatory mechanisms in atherosclerosis - A critical appraisal. The Semmelweis Symposium is an annual international activity at the Semmelweis University. In ...
figures - HAL
figures - HAL

... This is the second report of human anophthalmia-associated mutations of the RAX homeobox gene (11). While the parents were not carefully examined, they did not complain of any visual impairment at the time their child was evaluated. The proband was demonstrated to bear composite heterozygous mutatio ...
BIOLOGY 181 Lab # 10 Mendelian Genetics in Corn INTRODUCTION
BIOLOGY 181 Lab # 10 Mendelian Genetics in Corn INTRODUCTION

... recessive for that trait and your genotype is pp. If you lack a long palmar muscle in either arm, your genotype is PP. Presence of the trait in only one arm suggests that you are heterozygous for the trait, and your genotype is Pp. This trait is an example of incomplete dominance. ...
Article Fitness Trade-Offs Determine the Role of the Molecular
Article Fitness Trade-Offs Determine the Role of the Molecular

In vitro conjugal transfer of tetracycline resistance from Lactobacillus
In vitro conjugal transfer of tetracycline resistance from Lactobacillus

... Likewise, lactobacilli have also been extensively studied as plasmid recipients, such as for the broad-host-range plasmid pAML1 in the framework of optimising recombinant DNA technologies to improve strain properties as reviewed by Wang and Lee [3]. In this context, Reniero and co-workers reported t ...
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Genetic engineering



Genetic engineering, also called genetic modification, is the direct manipulation of an organism's genome using biotechnology. It is therefore a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms. New DNA may be inserted in the host genome by first isolating and copying the genetic material of interest using molecular cloning methods to generate a DNA sequence, or by synthesizing the DNA, and then inserting this construct into the host organism. Genes may be removed, or ""knocked out"", using a nuclease. Gene targeting is a different technique that uses homologous recombination to change an endogenous gene, and can be used to delete a gene, remove exons, add a gene, or introduce point mutations.An organism that is generated through genetic engineering is considered to be a genetically modified organism (GMO). The first GMOs were bacteria generated in 1973 and GM mice in 1974. Insulin-producing bacteria were commercialized in 1982 and genetically modified food has been sold since 1994. Glofish, the first GMO designed as a pet, was first sold in the United States December in 2003.Genetic engineering techniques have been applied in numerous fields including research, agriculture, industrial biotechnology, and medicine. Enzymes used in laundry detergent and medicines such as insulin and human growth hormone are now manufactured in GM cells, experimental GM cell lines and GM animals such as mice or zebrafish are being used for research purposes, and genetically modified crops have been commercialized.
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