Isolation of obligately alkaliphilic magnetotactic bacteria from
... MTB truly require highly alkaline conditions and are not simply surviving/growing in neutral pH micro-niches in their natural habitats. Alkaliphilic bacteria have a number of physiological problems to deal with living in such a harsh environment including keeping their intracellular pH around neutr ...
... MTB truly require highly alkaline conditions and are not simply surviving/growing in neutral pH micro-niches in their natural habitats. Alkaliphilic bacteria have a number of physiological problems to deal with living in such a harsh environment including keeping their intracellular pH around neutr ...
Synovial fibroblasts: key players in rheumatoid
... not merely the response to continuous stimulation by inflammatory mediators but constitute intrinsic features of these cells. This notion has mainly been derived from studies in the severe combined immunodeficient mouse (SCID) co-implantation model [10, 11]. This animal model for RA was developed to i ...
... not merely the response to continuous stimulation by inflammatory mediators but constitute intrinsic features of these cells. This notion has mainly been derived from studies in the severe combined immunodeficient mouse (SCID) co-implantation model [10, 11]. This animal model for RA was developed to i ...
In Vivo Release of Mitotic Silencing of Ribosomal Gene
... very sensitive to roscovitine (IC50 , 7 mM) when tested in vitro. However, because cdk2–cyclin E and cdk2–cyclin A are active at the G1/S transition and during S phase, respectively, the cdc2–cyclin B kinase is the only known kinase inhibited by roscovitine when mitotically synchronized cells are tr ...
... very sensitive to roscovitine (IC50 , 7 mM) when tested in vitro. However, because cdk2–cyclin E and cdk2–cyclin A are active at the G1/S transition and during S phase, respectively, the cdc2–cyclin B kinase is the only known kinase inhibited by roscovitine when mitotically synchronized cells are tr ...
Mochii - Salamander Genome Project
... muscle cells and their descendants by making a transgenic animal in which Cre recombinase driven by a muscle-specific promoter induced a recombination of loxP sites and the inheritable expression of a downstream green fluorescent protein (GFP) gene. If labeled cells had been found in differentiated ...
... muscle cells and their descendants by making a transgenic animal in which Cre recombinase driven by a muscle-specific promoter induced a recombination of loxP sites and the inheritable expression of a downstream green fluorescent protein (GFP) gene. If labeled cells had been found in differentiated ...
Identification of plant cytoskeleton-interacting proteins
... in frame to YFP, and a few clones were fused to YFP in a different open reading frame than the annotated gene, creating peptides with the ability to bind actin stress fibers. At present we consider the latter to be the background of the system. In the future when completing a comprehensive screen we ...
... in frame to YFP, and a few clones were fused to YFP in a different open reading frame than the annotated gene, creating peptides with the ability to bind actin stress fibers. At present we consider the latter to be the background of the system. In the future when completing a comprehensive screen we ...
Seed-coat Dormancy in Grevillea linearifolia
... permeability of the seed coat to an internal germination inhibitor, which subsequently escapes. This model was tested for in G. linearifolia by investigating the permeability of the seed coat to diffusion of high-molecularweight compounds, and whether this changed after exposure to fire cues. † Meth ...
... permeability of the seed coat to an internal germination inhibitor, which subsequently escapes. This model was tested for in G. linearifolia by investigating the permeability of the seed coat to diffusion of high-molecularweight compounds, and whether this changed after exposure to fire cues. † Meth ...
Cellular source and molecular form of TNF specify
... Figure 1. Maintenance of spleen microarchitecture is primarily dependent on TNF expressed by B cells with distinct contribution of T-cell–derived TNF. (A) Mice were immunized with i.p. SRBCs and spleen sections analyzed on day 8. B-TNF KO mice display reduced GCs, lack of polarized B-cell follicles, ...
... Figure 1. Maintenance of spleen microarchitecture is primarily dependent on TNF expressed by B cells with distinct contribution of T-cell–derived TNF. (A) Mice were immunized with i.p. SRBCs and spleen sections analyzed on day 8. B-TNF KO mice display reduced GCs, lack of polarized B-cell follicles, ...
SNARE Complex Proteins in Rat Mast Cells Phosphorylation of
... and basophils, indicating the involvement of these proteins in leukocyte degranulation. It has been well-established that leukocyte degranulation requires a higher chemoattractant concentration than chemotaxis, which may reflect the need for activation of multiple signaling pathways for degranulatio ...
... and basophils, indicating the involvement of these proteins in leukocyte degranulation. It has been well-established that leukocyte degranulation requires a higher chemoattractant concentration than chemotaxis, which may reflect the need for activation of multiple signaling pathways for degranulatio ...
Sequence-dependent synergism and antagonism between
... twice weekly, detaching them using trypsin 1X in PBS (Gibco). Trypsin activity was stopped using fresh culture medium. Cytotoxicity assay. Cells (7x10 3 /well in the two-drug combination) in their exponential cell growth were plated in 96-well microdilution plates (Corning). Following cell adherence ...
... twice weekly, detaching them using trypsin 1X in PBS (Gibco). Trypsin activity was stopped using fresh culture medium. Cytotoxicity assay. Cells (7x10 3 /well in the two-drug combination) in their exponential cell growth were plated in 96-well microdilution plates (Corning). Following cell adherence ...
NADPH oxidases as electrochemical generators to produce ion
... six transmembrane a-helices. EF—Ca2þ-binding EF-hand motif. PRR—proline-rich region, Src homology-3 domain. DUOX1 and DUOX2 were called dual oxidases because they have an additional ‘peroxidase’-like motif at their N-terminus on the outer surface of the membrane [27]. The product of the DUOXs is H2O ...
... six transmembrane a-helices. EF—Ca2þ-binding EF-hand motif. PRR—proline-rich region, Src homology-3 domain. DUOX1 and DUOX2 were called dual oxidases because they have an additional ‘peroxidase’-like motif at their N-terminus on the outer surface of the membrane [27]. The product of the DUOXs is H2O ...
Effect of Colchicine on Human Tissues.
... nodes, the epithelium of the fallopian tubes, endometrium, and the epithelium of the renal tubules. In the latter instance, an occasional multinucleated cell could be found (fig. 6). Diagnosis: Colchicine poisoning, scirrhous duct carcinoma of the right breast, hypoplasia of the bone marrow. Case 2. ...
... nodes, the epithelium of the fallopian tubes, endometrium, and the epithelium of the renal tubules. In the latter instance, an occasional multinucleated cell could be found (fig. 6). Diagnosis: Colchicine poisoning, scirrhous duct carcinoma of the right breast, hypoplasia of the bone marrow. Case 2. ...
Cell cycle behavior of human HP1 subtypes: distinct
... activities of chromosomes. It has been shown that mammalian HP1 homologues have a number of interacting partners such as the CAF-1 subunit p150 (Murzina et al., 1999), SP100 (Lehming et al., 1998; Seeler et al., 1998), TIF1-β (KAP-1) (Le Douarin et al., 1996; Ryan et al., 1999), Ku70 (Song et al., 2 ...
... activities of chromosomes. It has been shown that mammalian HP1 homologues have a number of interacting partners such as the CAF-1 subunit p150 (Murzina et al., 1999), SP100 (Lehming et al., 1998; Seeler et al., 1998), TIF1-β (KAP-1) (Le Douarin et al., 1996; Ryan et al., 1999), Ku70 (Song et al., 2 ...
An analysis of the response to gut induction in the C. elegans embryo
... EMS cells isolated before gut induction had occurred (1013 minutes before cytokinesis began in EMS) divided to form two daughters, both of which produced body wall muscle and pharyngeal muscle, but no gut or hypodermis, suggesting that both daughters differentiate as MS-like lineages, as MS normally ...
... EMS cells isolated before gut induction had occurred (1013 minutes before cytokinesis began in EMS) divided to form two daughters, both of which produced body wall muscle and pharyngeal muscle, but no gut or hypodermis, suggesting that both daughters differentiate as MS-like lineages, as MS normally ...
MESOPHYLL STRUCTURE AND CHLOROPLAST DENSITY IN
... conductance for CO2 and water vapor; increases of these parameters mean increasing leaf gas exchange and water use efficiency, and vice versa; plants that have maximal integral indexes grow in habitats at the ecological optimum for the given species (Pyankov et al., 1998; Ivanova and Pyankov, 2002). ...
... conductance for CO2 and water vapor; increases of these parameters mean increasing leaf gas exchange and water use efficiency, and vice versa; plants that have maximal integral indexes grow in habitats at the ecological optimum for the given species (Pyankov et al., 1998; Ivanova and Pyankov, 2002). ...
Localization of Phycoerythrin at the Lumenal Surface of the
... rinsed several times in buffer, and postfixed in buffered 1% osmium tetroxide for 1 h at 40C. After several buffer rinses, cells were taken through a graded ethanol series and embedded in Spurr's epoxy resin. Pale gold sections, cut with a diamond knife, were collected on Formvarcoated nickel grids. ...
... rinsed several times in buffer, and postfixed in buffered 1% osmium tetroxide for 1 h at 40C. After several buffer rinses, cells were taken through a graded ethanol series and embedded in Spurr's epoxy resin. Pale gold sections, cut with a diamond knife, were collected on Formvarcoated nickel grids. ...
General mikrobiology
... C. receptors to C3b component of complement D. all of these E. *MHC I class antigens 67. What’s next step of staining by Ziehl-Neelsen’s method after using sulphuric acid? A. Staining by ziehl’s phenol fuchsine B. Using alcohol C. Staining by mеthylene blue D. Using iodine solution E. *Washing with ...
... C. receptors to C3b component of complement D. all of these E. *MHC I class antigens 67. What’s next step of staining by Ziehl-Neelsen’s method after using sulphuric acid? A. Staining by ziehl’s phenol fuchsine B. Using alcohol C. Staining by mеthylene blue D. Using iodine solution E. *Washing with ...
to get the file - Oxford Brookes University
... factor attachment protein receptor (SNARE) protein NtSyr1 (NtSyp121) was probably involved in post-Golgi membrane traf®c (Geelen et al., 2002). Many key protein families controlling endomembrane traf®c can be predicted from their similarity to known yeast proteins and some can be readily modi®ed to ...
... factor attachment protein receptor (SNARE) protein NtSyr1 (NtSyp121) was probably involved in post-Golgi membrane traf®c (Geelen et al., 2002). Many key protein families controlling endomembrane traf®c can be predicted from their similarity to known yeast proteins and some can be readily modi®ed to ...
Sox3 - Prodinra
... central nervous system (CNS). Indeed, much of the CNS will develop from a subset of the non-ingressing cells later specified as neural precursors. Therefore, it is crucial to identify not only those factors that induce cell ingression at gastrulation but also those that prevent it, as protection fro ...
... central nervous system (CNS). Indeed, much of the CNS will develop from a subset of the non-ingressing cells later specified as neural precursors. Therefore, it is crucial to identify not only those factors that induce cell ingression at gastrulation but also those that prevent it, as protection fro ...
05_chapter 1
... poly(glycolic acid), poly(ethylene glycol), etc. are very promising in the formulation of dosage forms [Kim, 2004]. Chitin, poly (β-(1→4)-N-acetyl-D-glucosamine), first identified in 1884, is a naturally occurring polysaccharide consisting of amino sugars. This biopolymer is synthesized by an extrem ...
... poly(glycolic acid), poly(ethylene glycol), etc. are very promising in the formulation of dosage forms [Kim, 2004]. Chitin, poly (β-(1→4)-N-acetyl-D-glucosamine), first identified in 1884, is a naturally occurring polysaccharide consisting of amino sugars. This biopolymer is synthesized by an extrem ...
Defects in mesoderm, neural tube and vascular development in
... severe defects in mesodermally derived tissues. Notochord and somites are absent; the heart and ...
... severe defects in mesodermally derived tissues. Notochord and somites are absent; the heart and ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.