Gram cell staining
... according of differences in the structure of their cell walls ). The Gram staining procedure based on the fact that Crystal Violet stains the bacterial peptidoglycan cell wall. The Gram positive bacteria have a thick peptidoglican cell wall and no membrane on the outside of their cell wall (and cont ...
... according of differences in the structure of their cell walls ). The Gram staining procedure based on the fact that Crystal Violet stains the bacterial peptidoglycan cell wall. The Gram positive bacteria have a thick peptidoglican cell wall and no membrane on the outside of their cell wall (and cont ...
Examining the link between chromosomal instability and aneuploidy
... microscopy. Defects in mitotic processes such as spindle checkpoint function or multipolar spindle assembly predictably impair chromosome behavior by inducing premature entry into anaphase before chromosome alignment and multipolar anaphase, respectively. Thus, to identify the mechanism of chromosom ...
... microscopy. Defects in mitotic processes such as spindle checkpoint function or multipolar spindle assembly predictably impair chromosome behavior by inducing premature entry into anaphase before chromosome alignment and multipolar anaphase, respectively. Thus, to identify the mechanism of chromosom ...
Rabphilin mutants defective for Rab3 binding
... constructs attached on glutathione-Sepharose beads with extracts of PC12 cells. GST-GFP (a fusion protein between GST and the Green Fluorescent Protein) was used as a negative control. Because Rabphilin interacts preferentially with the GTP-bound form of Rab3A, the PC12 extracts were incubated with ...
... constructs attached on glutathione-Sepharose beads with extracts of PC12 cells. GST-GFP (a fusion protein between GST and the Green Fluorescent Protein) was used as a negative control. Because Rabphilin interacts preferentially with the GTP-bound form of Rab3A, the PC12 extracts were incubated with ...
Main text Introduction Mitosis (Gk. Mitos – warp thread or fiber and
... the G0 stage. On occasions, a distinction in terms is made between a G0 cell and a 'post-mitotic' cell (e.g., heart muscle cells and neurons), which will never enter the G1 phase, whereas other G0 cells may enter. Cells enter the G0 phase from a cell cycle checkpoint in the G1 phase, such as the ‘Re ...
... the G0 stage. On occasions, a distinction in terms is made between a G0 cell and a 'post-mitotic' cell (e.g., heart muscle cells and neurons), which will never enter the G1 phase, whereas other G0 cells may enter. Cells enter the G0 phase from a cell cycle checkpoint in the G1 phase, such as the ‘Re ...
Rearrangement of the Keratin Cytoskeleton after Combined
... The distribution and arrangement of cytokeratin in a control culture of HeLa cells that has been reacted with anti-akeratin antiserum and visualized using indirect immunofluorescence is shown in Figure 2a. The uniform-sized, wavyappearing keratin filaments are evenly distributed throughout the cytop ...
... The distribution and arrangement of cytokeratin in a control culture of HeLa cells that has been reacted with anti-akeratin antiserum and visualized using indirect immunofluorescence is shown in Figure 2a. The uniform-sized, wavyappearing keratin filaments are evenly distributed throughout the cytop ...
BSc.-I Medical - DAV College Jalandhar
... - Glycerine Jelly: Jelly is used for mounting. It is made up of gelatin. ...
... - Glycerine Jelly: Jelly is used for mounting. It is made up of gelatin. ...
Abl Protein-Tyrosine Kinase Inhibitor STI571 Inhibits In Vitro Signal
... CA) using the Bac-to-Bac expression system (Life Technologies Inc.). The proteins were then purified to near homogeneity by standard chromatographic techniques. Kinase inhibition was measured by detecting the decrease in phosphorylation of poly(Glu, Tyr) essentially as previously described for the e ...
... CA) using the Bac-to-Bac expression system (Life Technologies Inc.). The proteins were then purified to near homogeneity by standard chromatographic techniques. Kinase inhibition was measured by detecting the decrease in phosphorylation of poly(Glu, Tyr) essentially as previously described for the e ...
- eScholarship@UMMS
... mononucleate cells and 41.3% of binucleate cells incorporated BrdU (Table I). Time-lapse records revealed that 12 of 20 mononucleate cells went through a normal mitosis, whereas none of the six binucleate cells entered mitosis within 72 h of cytochalasin removal (Table I). The same phenomena were ob ...
... mononucleate cells and 41.3% of binucleate cells incorporated BrdU (Table I). Time-lapse records revealed that 12 of 20 mononucleate cells went through a normal mitosis, whereas none of the six binucleate cells entered mitosis within 72 h of cytochalasin removal (Table I). The same phenomena were ob ...
The cancer stem cell: premises, promises and challenges
... yet the tumors seemed hierarchically organized when tested functionally. This paper was rapidly followed by similar studies on other solid Revival of the CSC concept. In the early nineties, research on hemato tumors such as brain cancers25 and colon cancers26–28. For a comprepoietic stem cells was ...
... yet the tumors seemed hierarchically organized when tested functionally. This paper was rapidly followed by similar studies on other solid Revival of the CSC concept. In the early nineties, research on hemato tumors such as brain cancers25 and colon cancers26–28. For a comprepoietic stem cells was ...
Energy barriers and cell migration in densely packed tissues†
... analyzing isolated cells in controlled environments 7,8 , recent work has highlighted that cell motion in densely packed tissues is collective, and very different from isolated cell motion. In densely packed or confluent tissues (no gaps between cells) researchers have discovered signatures of colle ...
... analyzing isolated cells in controlled environments 7,8 , recent work has highlighted that cell motion in densely packed tissues is collective, and very different from isolated cell motion. In densely packed or confluent tissues (no gaps between cells) researchers have discovered signatures of colle ...
Low-cost `plastic` solar cells: a dream becoming a reality Alan Heeger
... components both of which are soluble in the same solvent. When cast as films from solution, the phases of the two components separate as the solvent quickly evaporates. After separation, the two components self-assemble into the material depicted in Figure 2. This so-called bulk heterojunction mater ...
... components both of which are soluble in the same solvent. When cast as films from solution, the phases of the two components separate as the solvent quickly evaporates. After separation, the two components self-assemble into the material depicted in Figure 2. This so-called bulk heterojunction mater ...
meeting report - The Plant Cell
... 1998). Although the cytokinetic mechanisms unique to plant cells are not fully elaborated, many ultrastructural features of dividing plant cells are well known. The preprophase band, for instance, is a microtubular array that determines the future division plane. At telophase, moreover, a cell plate ...
... 1998). Although the cytokinetic mechanisms unique to plant cells are not fully elaborated, many ultrastructural features of dividing plant cells are well known. The preprophase band, for instance, is a microtubular array that determines the future division plane. At telophase, moreover, a cell plate ...
Silibinin improves the cytotoxicity of methotrexate in
... patients. Drug resistance concomitant with invasion has been found in many cancer cells isolated from patients with different types of tumors;1that cells have these properties may be due to anincrease in metastatic potential following chemotherapeuticinsults.2 Thus, there is an urgent ...
... patients. Drug resistance concomitant with invasion has been found in many cancer cells isolated from patients with different types of tumors;1that cells have these properties may be due to anincrease in metastatic potential following chemotherapeuticinsults.2 Thus, there is an urgent ...
S tem cells and cardiac disorders: an appraisal
... 1–5) has been reported to inhibit cardiogenesis [4] and induces the formation of neuronal and glial cells from pluripotent ES cells and embryoid bodies in parallel with increased activity of PAX6, a transcription factor involved in central nervous system development [9]. RA is pivotally involved in ...
... 1–5) has been reported to inhibit cardiogenesis [4] and induces the formation of neuronal and glial cells from pluripotent ES cells and embryoid bodies in parallel with increased activity of PAX6, a transcription factor involved in central nervous system development [9]. RA is pivotally involved in ...
MOVEMENT OF SUBSTANCES ACROSS THE PLASMA MEMBRANE …
... Small non-polar (hydrophobic) molecules that are lipid-soluble, such as fatty acids, glycerol, steroid, vitamin A, D, E and K. Small unchanged molecules, such as water, oxygen and carbon dioxide. These molecules are small enough to squeeze through between the phospholipid gaps by simple diffusion or ...
... Small non-polar (hydrophobic) molecules that are lipid-soluble, such as fatty acids, glycerol, steroid, vitamin A, D, E and K. Small unchanged molecules, such as water, oxygen and carbon dioxide. These molecules are small enough to squeeze through between the phospholipid gaps by simple diffusion or ...
The term “fluid mosaic model” refers to ______.
... b. In cold climates, more saturated fats with kinked tails are needed to maintain the fluidity of the cell membranes. c. In warm climates, more unsaturated fats with kinked tails are needed to maintain the fluidity of the cell membranes. d. In warm climates, more saturated fats with kinked tails are ...
... b. In cold climates, more saturated fats with kinked tails are needed to maintain the fluidity of the cell membranes. c. In warm climates, more unsaturated fats with kinked tails are needed to maintain the fluidity of the cell membranes. d. In warm climates, more saturated fats with kinked tails are ...
Osmosis and Diffusion Lab Instructions: Refer to background
... magnification. Sketch and describe the appearance of the onion cells in your lab notebook. 2. Add 2 or 3 drops of 15% NaCl solution to one edge of the cover slip. Draw this salt solution across the slide by touching a piece of paper towel to the fluid under the opposite edge of the cover slip. Sketc ...
... magnification. Sketch and describe the appearance of the onion cells in your lab notebook. 2. Add 2 or 3 drops of 15% NaCl solution to one edge of the cover slip. Draw this salt solution across the slide by touching a piece of paper towel to the fluid under the opposite edge of the cover slip. Sketc ...
V. CELL TRANSPORT, cont
... Allows for movement Attachment site for organelles, enzymes More extensive in animal cells Composed of three types of proteins ...
... Allows for movement Attachment site for organelles, enzymes More extensive in animal cells Composed of three types of proteins ...
Chapters 4 and 5 Cell Structures, Functions and Transport
... are made up of two parts, called subunits. They get their names from their size. One unit is larger than than the other so they are called large and small subunits. Both these subunits are necessary for protein synthesis in the cell. When the two units are docked together with a special information ...
... are made up of two parts, called subunits. They get their names from their size. One unit is larger than than the other so they are called large and small subunits. Both these subunits are necessary for protein synthesis in the cell. When the two units are docked together with a special information ...
Brown and Goldstein: The Cholesterol Chronicles
... affliction. At a time when the now-ubiquitous cholesterol-lowering drugs called statins were yet to be discovered, his treatment over the next several years included a low-fat diet, cholestyramine (a drug that shunts excess cholesterol toward bile acid production), and nicotinic acid, which helps low ...
... affliction. At a time when the now-ubiquitous cholesterol-lowering drugs called statins were yet to be discovered, his treatment over the next several years included a low-fat diet, cholestyramine (a drug that shunts excess cholesterol toward bile acid production), and nicotinic acid, which helps low ...
Selective Cytotoxicity of Rhodium Metalloinsertors in Mismatch
... were stained with propidium iodide (PI) and analyzed by flow cytometry. The PI fluorescence reports the amount of DNA in each cell and follows a bimodal distribution, where the first peak contains cells with one copy of the genome, i.e., cells in G0/G1-phase, and the second peak contains cells with ...
... were stained with propidium iodide (PI) and analyzed by flow cytometry. The PI fluorescence reports the amount of DNA in each cell and follows a bimodal distribution, where the first peak contains cells with one copy of the genome, i.e., cells in G0/G1-phase, and the second peak contains cells with ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.