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The CRISPR-Cas technique has been used to generate gene knock
The CRISPR-Cas technique has been used to generate gene knock

... in-frame and then expressed. The mCherry is a monomeric fluorescent protein with peak absorption/emission at 587 nm and 610 nm, respectively. Cells with mCherry fluorescence will be collected by cell sorter to enrich the population of cells with possible editing in the endogenous gene. After 3 days ...
Tissue Repair - Johns Hopkins Medicine
Tissue Repair - Johns Hopkins Medicine

... “let’s repair diseased tissue with tissue stem cells” ...
Cell Structure and Function
Cell Structure and Function

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ACTIVE TRANSPORT
ACTIVE TRANSPORT

... change the shape of a carrier protein so that required particles move from an area of low concentration to an area of high concentration. • Unlike diffusion, particles go against the concentration gradient. • It is analogous to rowing a boat upstream against the current (concentration gradient). ...
Animal Cells: Cells Without Walls Questions
Animal Cells: Cells Without Walls Questions

... move itself from place to place; plants cannot. Plants have very rigid cells. They keep their shape. This is because plant cells have a thick cell wall that protects and strengthens the outside of the cell. Animal cells do not have a cell wall. They have a thin cell membrane that forms the outside o ...
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... the first to become macroscopically visible when histiotypic cultures are transplanted in mass cultures, appears in close association with hollow vesicles lined by epithelial cells; these vesicles show the characteristic features of yolk-sac endoderm of normal mouse embryo. In addition, the structur ...
C, O, N - Madeira City Schools
C, O, N - Madeira City Schools

... because they have no nucleus B. Binary Fission: asexual reproduction (there is no exchange of genetic information) 1. Chromosome is replicated 2. Copies of chromosome attach to the cell membrane 3. Cell grows and the copies separate 4. Cell divides in two as a partition forms down the middle ...
Cell Structure Vocabulary
Cell Structure Vocabulary

... extraction of energy from nutrients, or storage. Examples of organelles include ribosome, mitochondria, and vacuole. Sentence: ______________________________________________________________________________________ Example:______________________________________________________________________________ ...
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Chapter 5 Organelles
Chapter 5 Organelles

... Do brain cells have the same internal structures as your other cells? Yes. Although brain cells look quite different from your other cells, they have the same internal structures as other cells. They need the same structures because they need to perform the same tasks, such as making proteins and ob ...
3 Bio Eukaryotic Cell Structure and Function (Ch 7.2)
3 Bio Eukaryotic Cell Structure and Function (Ch 7.2)

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Cells, Classification, and Levels of Organization Review
Cells, Classification, and Levels of Organization Review

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Visual PPT Quiz # 5

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Optimal Conditions for Labelling of 3T3 Fibroblasts with Magneto
Optimal Conditions for Labelling of 3T3 Fibroblasts with Magneto

... Iron uptake per cell: The combined results of the intracellular iron determination together and the BCA protein assay make it possible to determine the average amount of iron taken up per cell. To do so, the results of the BCA assay must be transformed to convert a certain amount of protein into a c ...
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Intracellular trafficking and mis-trafficking of disease
Intracellular trafficking and mis-trafficking of disease

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... sciences has not led to a cure for cancer may seem disappointing to the general public; it has, however, led scientists to a much better understanding of the ‘problem’ of cancer. The discovery of the genetic basis for tumorigenesis, along with the advent of ‘biotechnology’ holds great promise that t ...
cell webquest 2015
cell webquest 2015

... 2. Which type of cell forms a protective layer for your body?____________________________ 3. Put the level of organization in order from smallest to largest._________________________ 4. What is the function of a nerve cell? ___________________________________________ 5. Which of the following is an ...
Prokaryotic and eukaryotic cells. Power Point
Prokaryotic and eukaryotic cells. Power Point

... Key Point #1: The function of a cell (what it does) is determined by its structure (what it is made of) DNA (deoxyribonucleic acid) is the genetic code that instructs the cell how to run (it’s what makes you “you”) Organelle: Part of a cell ...
Eukaryotic Cells and Cell Organelles
Eukaryotic Cells and Cell Organelles

... Other organelles have various functions. Some organelles do jobs other than making proteins. Mitochondria  Mitochondria are bean-shaped organelles that produce chemical energy that is usable by a cell. They have two membranes. The inner membrane has lots of folds that form compartments. Mitochondria ...
Differentiate between active and passive transport
Differentiate between active and passive transport

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The Endomembrane System
The Endomembrane System

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IGCSE BIOLOGY 2.1 Cellular organization State that living
IGCSE BIOLOGY 2.1 Cellular organization State that living

... Do not have a cell wall and therefore require either external or internal support from some kind of skeleton. Have a shape related to their function. Animals must eat their food because they cannot photosynthesize. Animal cells may have several small vacuoles, or none at all. ...
IGCSE BIOLOGY 2.1 Cellular organization State that living
IGCSE BIOLOGY 2.1 Cellular organization State that living

... Do not have a cell wall and therefore require either external or internal support from some kind of skeleton. Have a shape related to their function. Animals must eat their food because they cannot photosynthesize. Animal cells may have several small vacuoles, or none at all. ...
Ribosomes
Ribosomes

...  Inside the chloroplasts are small flat disk-shaped structures called thylakoids that convert light from the sun into usable energy.  The thylakoids contain a special pigment called chlorophyll that traps the sunlight.  Chloroplasts are only found in plants, some protists and bacteria, but not an ...
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Cell culture



Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.
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