Expression of a constitutively activated form of protein kinase B (c
... of LPL( lane 12). aP2 induction is seen in one of the two pools (lane 2). No such induction of LPL or aP2 was observed for control cells (lane 3). Control cells were, however, completely capable of undergoing adipogenesis when exposed to a differentiation medium containing insulin (data not shown). ...
... of LPL( lane 12). aP2 induction is seen in one of the two pools (lane 2). No such induction of LPL or aP2 was observed for control cells (lane 3). Control cells were, however, completely capable of undergoing adipogenesis when exposed to a differentiation medium containing insulin (data not shown). ...
Neurons are highly specialized cells.
... Neurons receive and transmit signals. • Resting potential means no signal is being transmitted. – more Na+ outside of cell – more K+ inside of cell ...
... Neurons receive and transmit signals. • Resting potential means no signal is being transmitted. – more Na+ outside of cell – more K+ inside of cell ...
Microfilaments Intermediate filaments
... Recent advances in light microscopy Confocal microscopy and deconvolution microscopy provide sharper images of three-dimensional tissues and cells New techniques for labeling cells improve resolution ...
... Recent advances in light microscopy Confocal microscopy and deconvolution microscopy provide sharper images of three-dimensional tissues and cells New techniques for labeling cells improve resolution ...
Designing cell lines for viral vaccine production: where do we stand?
... general, a variety of cells/hosts will be needed to efficiently respond to variations of the viruses or virus subtypes. Such an approach offers potential solutions to problems such as lower performance for some subtypes, or if allergies or contamination issues occur. 4.2 Selection of tissue The choi ...
... general, a variety of cells/hosts will be needed to efficiently respond to variations of the viruses or virus subtypes. Such an approach offers potential solutions to problems such as lower performance for some subtypes, or if allergies or contamination issues occur. 4.2 Selection of tissue The choi ...
The Human Cell Membrane
... All cells are surrounded by a cell membrane (also called the plasma membrane). This is a biological membrane or biomembrane consisting of a double layer of lipids in which proteins are located. The cell membrane keeps the components of the cell isolated from the external environment. It also serves ...
... All cells are surrounded by a cell membrane (also called the plasma membrane). This is a biological membrane or biomembrane consisting of a double layer of lipids in which proteins are located. The cell membrane keeps the components of the cell isolated from the external environment. It also serves ...
further characterization of the f1
... fetal bovine serum. After 2-3 h incubation, M cells were separated by selective detachment in regular prewarmed tricine EM without serum . I cells were vigorously rinsed to dislodge residual M cells. Under these labeling conditions, all collected M cells are representative of that portion of G2 cell ...
... fetal bovine serum. After 2-3 h incubation, M cells were separated by selective detachment in regular prewarmed tricine EM without serum . I cells were vigorously rinsed to dislodge residual M cells. Under these labeling conditions, all collected M cells are representative of that portion of G2 cell ...
Cell Wall
... causes the cell wall to weaken and lyse.[18] The lysozyme enzyme can also damage bacterial cell walls. There are broadly speaking two different types of cell wall in bacteria, called Gram-positive and Gramnegative. The names originate from the reaction of cells to the Gram stain, a test long-employe ...
... causes the cell wall to weaken and lyse.[18] The lysozyme enzyme can also damage bacterial cell walls. There are broadly speaking two different types of cell wall in bacteria, called Gram-positive and Gramnegative. The names originate from the reaction of cells to the Gram stain, a test long-employe ...
Unit 1 Biology 3
... live in still water do not generally populate running waters. It is the presence of a rigid wall that survives after the death of the diatom cell that leaves a trace, and this can be followed long after the death of a person. The popular face of forensic science, as it is portrayed in many televisio ...
... live in still water do not generally populate running waters. It is the presence of a rigid wall that survives after the death of the diatom cell that leaves a trace, and this can be followed long after the death of a person. The popular face of forensic science, as it is portrayed in many televisio ...
GenII cells alld early de\,c/0l`lIlell! 227S Introduction.Neurotrophic
... Seidl, K. and Holstein, A.F. (1990). Evidence for the presence of nerve growth factor (NGF) and NGF receptors in human testis. Cell. Tissue Res. 261:549-554. Wilkinson, D., Bailes, J., Champion, J. and McMahon, A. (1987). A molecular analysis of mouse development from 8 to 10 days post coitum detect ...
... Seidl, K. and Holstein, A.F. (1990). Evidence for the presence of nerve growth factor (NGF) and NGF receptors in human testis. Cell. Tissue Res. 261:549-554. Wilkinson, D., Bailes, J., Champion, J. and McMahon, A. (1987). A molecular analysis of mouse development from 8 to 10 days post coitum detect ...
Microbial Discovery Activity
... Students should know the difference in cellular composition of prokaryotic and eukaryotic cells and that certain structures or properties confer particular abilities on the cells, such as the ability of the acid-fast cell wall to resist uptake of certain drugs, or the ability of an endospore to allo ...
... Students should know the difference in cellular composition of prokaryotic and eukaryotic cells and that certain structures or properties confer particular abilities on the cells, such as the ability of the acid-fast cell wall to resist uptake of certain drugs, or the ability of an endospore to allo ...
The Biotechnology Century and Its Workforce
... What type of response do the immunocompetent cells in transplanted bone marrow mount against the tissue into which they have been transplanted? a. b. c. d. ...
... What type of response do the immunocompetent cells in transplanted bone marrow mount against the tissue into which they have been transplanted? a. b. c. d. ...
Investigations in Science 7 - Organelle poster tiered task
... Instructions: You are about to take a tour, either real or imagined, around your school. You will pass through parts of the school that are familiar, yet you must think of them in new ways. You are going to develop analogies, or comparisons, to the parts of your school and the organelles of a cell. ...
... Instructions: You are about to take a tour, either real or imagined, around your school. You will pass through parts of the school that are familiar, yet you must think of them in new ways. You are going to develop analogies, or comparisons, to the parts of your school and the organelles of a cell. ...
A Mechanism Linking Extra Centrosomes to Chromosomal Instability
... extra centrosomes may, at low frequency, arise by other mechanisms. For example, we occasionally observed single chromosomes bi-orienting between two inefficiently clustered centrosomes even after all other chromosomes had aligned at the metaphase plate (see Fig 3a, top row, middle panel). Presumabl ...
... extra centrosomes may, at low frequency, arise by other mechanisms. For example, we occasionally observed single chromosomes bi-orienting between two inefficiently clustered centrosomes even after all other chromosomes had aligned at the metaphase plate (see Fig 3a, top row, middle panel). Presumabl ...
Chapter 14.
... Human development more tolerant of wrong numbers in sex chromosome But produces a variety of distinct syndromes in humans ...
... Human development more tolerant of wrong numbers in sex chromosome But produces a variety of distinct syndromes in humans ...
Nice, T.J., W. Deng, L. Coscoy and D.H. Raulet. 2010. Stress-regulated targeting of the NKG2D ligand Mult1 by a membrane-associated RING-CH family E3 ligase. J Immunol 185:5369-5376.
... and nine known human ligands (RAET1a–RAET1g [also known as UL-16 binding proteins], MICA, and MICB) that are all distantly related to MHC class I. However, because of their relatively rapid rate of evolution, it has not been possible to draw direct lines of homology between individual mouse and huma ...
... and nine known human ligands (RAET1a–RAET1g [also known as UL-16 binding proteins], MICA, and MICB) that are all distantly related to MHC class I. However, because of their relatively rapid rate of evolution, it has not been possible to draw direct lines of homology between individual mouse and huma ...
Chapter 4: Tissues
... B. Connective Tissue Cells 1. Fibroblasts large, flat cells with branching processes present in several connective tissues, and usually the most numerous. Fibroblasts migrate through the connective tissue, secreting the fibers and ground substance of the extracellular matrix. 2. Macrophages devel ...
... B. Connective Tissue Cells 1. Fibroblasts large, flat cells with branching processes present in several connective tissues, and usually the most numerous. Fibroblasts migrate through the connective tissue, secreting the fibers and ground substance of the extracellular matrix. 2. Macrophages devel ...
IV. Principles of Serological Testing in Immunohematology
... May be produced by hybridomas, rabbits, or a mixture. Used for routine compatibility testing, antibody identification and DAT. Most important function is to detect IgG antibodies coating the cells. The importance of the presence of anti-complement in AHG serum is very controversial for routine compa ...
... May be produced by hybridomas, rabbits, or a mixture. Used for routine compatibility testing, antibody identification and DAT. Most important function is to detect IgG antibodies coating the cells. The importance of the presence of anti-complement in AHG serum is very controversial for routine compa ...
FR in detergent-insoluble complexes - Journal of Cell Science
... solubilization at low temperature was evaluated in IGROV1 cells. Based on the [3H]FA distribution in the soluble (S) and insoluble (I) fractions, 40 to 60% of FR was found in the TX100 S fraction and the remaining radioactivity was almost completely solubilized by a second treatment with OG (TX100 I ...
... solubilization at low temperature was evaluated in IGROV1 cells. Based on the [3H]FA distribution in the soluble (S) and insoluble (I) fractions, 40 to 60% of FR was found in the TX100 S fraction and the remaining radioactivity was almost completely solubilized by a second treatment with OG (TX100 I ...
acid phosphatase localization in pas
... Second, we have not determined the pH optimum for acid phosphatase activity in G. polyedra. If it differs very much from pH 5-0, localizable activity would be lower than the optimum. Although we have not seen a study in which the pH optimum for acid phosphatase activity was much higher, Miiller (197 ...
... Second, we have not determined the pH optimum for acid phosphatase activity in G. polyedra. If it differs very much from pH 5-0, localizable activity would be lower than the optimum. Although we have not seen a study in which the pH optimum for acid phosphatase activity was much higher, Miiller (197 ...
staxanthin, a kotenoid without Vitamin A Activity,
... 2 (ThI and Th2) Th cell clones. The Ag for the Thl and 7712 clones were pigeon cytochrome C and rabbit y-globulin, respectively. Astaxanthin and &carotene augmented the number of IgM antibody (Ab)-secreting cells when unprimed B cells were incubated with Th clones and stimulated with suboptimal dose ...
... 2 (ThI and Th2) Th cell clones. The Ag for the Thl and 7712 clones were pigeon cytochrome C and rabbit y-globulin, respectively. Astaxanthin and &carotene augmented the number of IgM antibody (Ab)-secreting cells when unprimed B cells were incubated with Th clones and stimulated with suboptimal dose ...
Head/tail interaction of vinculin influences cell mechanical behavior
... that focal adhesion formation depends on internal tension [2,11,24,25]. We determined the number of focal adhesions in MEF cell lines of similar spreading area (Fig. 3). MEFvin+/+ and MEFRescue cells showed similar density of focal adhesions, whereas MEFvinA50I and MEFvin / cells showed between 40%– ...
... that focal adhesion formation depends on internal tension [2,11,24,25]. We determined the number of focal adhesions in MEF cell lines of similar spreading area (Fig. 3). MEFvin+/+ and MEFRescue cells showed similar density of focal adhesions, whereas MEFvinA50I and MEFvin / cells showed between 40%– ...
CKIP-1, a proinflammatory protein in macrophages interferes with
... cytokine TNF have been reported by several laboratories. Although TNF reverse signaling has been emerging as an important phenomenon in the immune response, its molecular basis remains elusive. Here we examined the role of CKIP-1, an interacting partner of the intracellular domain of tmTNF in inflam ...
... cytokine TNF have been reported by several laboratories. Although TNF reverse signaling has been emerging as an important phenomenon in the immune response, its molecular basis remains elusive. Here we examined the role of CKIP-1, an interacting partner of the intracellular domain of tmTNF in inflam ...
PROTISTA
... and digest their food, surrounding a cell or particle and then taking it inside themselves to form a food vacuole. A food vacuole is a small cavity in the cytoplasm that ...
... and digest their food, surrounding a cell or particle and then taking it inside themselves to form a food vacuole. A food vacuole is a small cavity in the cytoplasm that ...
Lab Practical 3 Review
... -‐ Fluid Thioglycollate Tubes: Thioglycollate broth (Fluid Thioglycollate Medium) is a medium designed to test the aerotolerance of bacteria. It contains the dye resazurin, which is an indicator for the prese ...
... -‐ Fluid Thioglycollate Tubes: Thioglycollate broth (Fluid Thioglycollate Medium) is a medium designed to test the aerotolerance of bacteria. It contains the dye resazurin, which is an indicator for the prese ...
Human TH17 Immune Cells Specific for the
... patients (Fig. 1A). In one of them, patient NA171, a significant IL-17 response was also detected. Simultaneous assessment of IFN-g and IL-17 secretion revealed 3 distinct subpopulations, 2 major ones secreting IL-17 or IFN-g alone and one cosecreting IL-17 and IFN-g (Fig. 1B). CD4þ memory T cells ex ...
... patients (Fig. 1A). In one of them, patient NA171, a significant IL-17 response was also detected. Simultaneous assessment of IFN-g and IL-17 secretion revealed 3 distinct subpopulations, 2 major ones secreting IL-17 or IFN-g alone and one cosecreting IL-17 and IFN-g (Fig. 1B). CD4þ memory T cells ex ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.