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CRISPR/Cas9 Screens Reveal Requirements for Host Cell Sulfation
CRISPR/Cas9 Screens Reveal Requirements for Host Cell Sulfation

... endogenous levels of GAGs are not limiting for T3SS1-dependent cell death (Figure 3A). These data also indicate that structurally diverse sulfated GAGs associated with the surface of host cells can promote V. parahaemolyticus T3SS1 toxicity even in the absence of covalent linkages between GAGs and s ...
COPY FACE SHEET Dr. Marks Room 217 Cell Membrane Key Ideas
COPY FACE SHEET Dr. Marks Room 217 Cell Membrane Key Ideas

... •This pump is one of the most important carrier proteins in animal cells. It prevents sodium ions from building up in the cell, resulting in osmosis into the cell. ...
3.2 Cell Organelles Cells have an internal structure.
3.2 Cell Organelles Cells have an internal structure.

... Plant cells have cell walls and chloroplasts. • A cell wall provides rigid support. • Chloroplasts convert solar energy to chemical energy. ...
Paclitaxel-induced microtubule stabilization causes mitotic block
Paclitaxel-induced microtubule stabilization causes mitotic block

2-Cell Injury L1, 2008
2-Cell Injury L1, 2008

... ATP depletion and decreased ATP synthesis are frequently associated with both hypoxic and chemical (toxic) injury Depletion of ATP to <5% to 10% of normal levels has widespread effects on many critical cellular systems: ◦ Plasma membrane energy-dependent sodium pump is reduced, resulting in cell swe ...
Inactivation of S-adenosylhomocysteine hydrolase intact cells
Inactivation of S-adenosylhomocysteine hydrolase intact cells

Mast cells and dendritic cells form synapses that facilitate antigen
Mast cells and dendritic cells form synapses that facilitate antigen

... al., 2006; Cleyrat et al., 2013). To determine whether the actMCs are indeed polarized during their interactions with the imDCs, we examined their cytoskeletal reorganization in relation to the cell–cell contact site. For these experiments, MCs were activated in the presence of imDCs, and samples we ...
Unit 5
Unit 5

... chloroplast, cytoskeleton, centrioles, nucleolus, chromosomes, nuclear membrane, cell wall, cell membrane [active and passive transport], cytosol) • Components of mobility (e.g., cilia, flagella, pseudopodia) c. Describe and differentiate among the organizational levels of organisms (e.g., cells, ti ...
Life after meiosis: patterning the angiosperm male gametophyte
Life after meiosis: patterning the angiosperm male gametophyte

... germ cell to progress through S-phase [25]. Conversely, the persistence of KRP6/KRP7 in the vegetative cell is proposed to maintain inhibition of CDKA activity and vegetative cell-cycle progression. Germline-specific expression of FBL17 thus enables differential control of the cell cycle in the germ ...
Effect of Coenzyme Q10 supplementation on mitochondrial electron
Effect of Coenzyme Q10 supplementation on mitochondrial electron

... Int J Biochem Cell Biol. 2014 May;50:60-3. doi: 10.1016/j.biocel.2014.02.003. Epub 2014 Feb 15. ...
Retroviral Expression Vector
Retroviral Expression Vector

... the generalized somatic mutation seen throughout the variable regions of immunoglobulin genes (2). Later, it became evident that this somatic mutation process takes place in the later stages of B-lymphoid development (13), when TdT is no longer present (18), and somatic mutation probably does not oc ...
Sickle cell anaemia
Sickle cell anaemia

... • A red blood cell survives 120 days • The spleen (part of the reticulo-endothelial system) is the main organ which removes old and damaged RBCs from the circulation. • Breakdown of RBCs can exceed the rate that the body can make RBCs and so anemia can ...
Nephrogenesis
Nephrogenesis

... Overview of principal events in early nephrogenesis. Ureteric bud, an offspring of Wolffian duct, invades mesenchymal blastema (left) and initiates reciprocal signaling (middle) between epithelial (ductal) and mesenchymal (metanephrogenic) cell types. Receptor tyrosine kinases are expressed almost ...
Heading forwards: anterior visceral endoderm migration in
Heading forwards: anterior visceral endoderm migration in

... region of three columnar Epi-VE cells. One AVE cell is outlined in green which relates to panel (c). The apical–basal polarity of the Epi-VE cells is shown via the coloured lines: purple, the basolateral domain; blue, the apical junctional domain; orange, the apicolateral domain. (b) En face surface ...
secretion and endocytosis in insulin
secretion and endocytosis in insulin

... IOtGVl~E8 Part of a cell from an animal injected with insulin 4 h before fixation. Only a very few secretory granules remain in the cytoplasm (S). N indicates the nucleus, M mitochondria, and G Golgi apparatus. At the cell surface, the plasma membrane shows some foldings or convolutions (P). The bod ...
Exposure to UV light causes increased biotinylation of histones in
Exposure to UV light causes increased biotinylation of histones in

... degraded in response to UV exposure, as judged by Western blot analysis and carboxylase activities. Mitochondrial integrity decreased in response to UV exposure (as judged by confocal microscopy), facilitating the release of breakdown products of carboxylases from mitochondria. Biotinylation of hist ...
Cell Cycle Control in the Fission Yeast
Cell Cycle Control in the Fission Yeast

... using an elutriator rotor. A similar result was obtained for the cdcl0 gene transcript. This has been isolated and subcloned to a 2.6 kb DNA fragment encoding a 2.7 kb polyadenylated RNA transcript (Aves et al., 1985). The level of this RNA is also unchanged during the shift between exponential grow ...
How Life Began 2014.notebook
How Life Began 2014.notebook

... Heterotroph Hypothesis  ­ Oparin and Haldane  ...
Multipotent stem cells from the dorsal aorta
Multipotent stem cells from the dorsal aorta

... possibly originate from a common ancestor (a ‘mesoangioblast’, rather than an hemo-angioblast) would leave the vessel and adopt the fate of the tissue where the vessel has entered (Bianco and Cossu, 1999). This fate choice would depend upon local signals emanating from differentiating cells of that ...
Cell Transport Powerpoint
Cell Transport Powerpoint

... Hypertonic Solutions: contain a high concentration of solute relative to another solution (e.g. the cell's cytoplasm). When a cell is placed in a hypertonic solution, the water diffuses out of the cell, causing the cell to shrivel. Hypotonic Solutions: contain a low concentration of solute relative ...
laboratory science
laboratory science

... that did not contain A2E (no A2E, no IOL) remained viable, while blue light-illuminated (430 nm peak with a bandwidth of 60 nm, 8 mW/cm2) RPE that had previously accumulated A2E underwent marked cell death. In the present experiments, 41.1% ⫾ 4.1% (3 experiments) of the A2E-laden cells in a field of ...
what is the sturcture of a virus?
what is the sturcture of a virus?

... Not all viruses kill the cells they infect. Some viruses go through a lysogenic cycle. A lysogenic cycle is a replication cycle in which the virus’s nucleic acid is integrated into the chromosome of the host cell. The lysogenic cycle begins the same way as a lytic cycle. The virus attaches to the ho ...
Strategies for the Allocation of Resources under Sulfur Limitation in
Strategies for the Allocation of Resources under Sulfur Limitation in

... cultures adapted to high and low sulfur was investigated (Fig. 6). The maximal photosynthetic rate (Pmax) under sulfur-limited conditions was found to be approximately 3 times lower as compared with the Pmax of high sulfate grown cells. The Pmax was not affected by the sulfate concentration used dur ...
review cell division from a genetic perspective
review cell division from a genetic perspective

... effects upon meiosis, have been examined. Six of them produce mitotic chromosome instability, and at least three of these are also abnormally sensitive to mutagenic agents, suggesting defects in DNA repair. Four influence the frequency of chromosome nondisjunction and/or chromosome loss. The mutants ...
PDF
PDF

... cortical component of the mother cell (for example, produces daughter cells, AB and CD, that are unequal by Conklin, 1905; Boveri, 1910; Spemann, 1938; Shimizu, three criteria. Cell CD is larger than cell AB, it inherits 1982a, b; Milhausen and Agabian, 1983; Gober et cd, essentially all of the telo ...
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Amitosis

Amitosis (a- + mitosis) is absence of mitosis, the usual form of cell division in the cells of eukaryotes. There are several senses in which eukaryotic cells can be amitotic. One refers to capability for non-mitotic division and the other refers to lack of capability for division. In one sense of the word, which is now mostly obsolete, amitosis is cell division in eukaryotic cells that happens without the usual features of mitosis as seen on microscopy, namely, without nuclear envelope breakdown and without formation of mitotic spindle and condensed chromosomes as far as microscopy can detect. However, most examples of cell division formerly thought to belong to this supposedly ""non-mitotic"" class, such as the division of unicellular eukaryotes, are today recognized as belonging to a class of mitosis called closed mitosis. A spectrum of mitotic activity can be categorized as open, semi-closed, and closed mitosis, depending on the fate of the nuclear envelope. An exception is the division of ciliate macronucleus, which is not mitotic, and the reference to this process as amitosis may be the only legitimate use of the ""non-mitotic division"" sense of the term today. In animals and plants which normally have open mitosis, the microscopic picture described in the 19th century as amitosis most likely corresponded to apoptosis, a process of programmed cell death associated with fragmentation of the nucleus and cytoplasm. Relatedly, even in the late 19th century cytologists mentioned that in larger life forms, amitosis is a ""forerunner of degeneration"".Another sense of amitotic refers to cells of certain tissues that are usually no longer capable of mitosis once the organism has matured into adulthood. In humans this is true of various muscle and nerve tissue types; if the existing ones are damaged, they cannot be replaced with new ones of equal capability. For example, cardiac muscle destroyed by heart attack and nerves destroyed by piercing trauma usually cannot regenerate. In contrast, skin cells are capable of mitosis throughout adulthood; old skin cells that die and slough off are replaced with new ones. Human liver tissue also has a sort of dormant regenerative ability; it is usually not needed or expressed but can be elicited if needed.
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