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Hijacking of eukaryotic functions by intracellular bacterial pathogens
Hijacking of eukaryotic functions by intracellular bacterial pathogens

... Studies using tissue culture cells have shown that InlB is essential for Listeria uptake by most non-phagocytic cell types, such as hepatocytes, endothelial cells, fibroblasts and certain epithelial cell lines. In contrast, InlA-mediated invasion is apparently restricted to cells expressing E-cadher ...
Plant Cytoskeleton: DELLA Connects Gibberellins to Microtubules
Plant Cytoskeleton: DELLA Connects Gibberellins to Microtubules

... these conditions, the cortical microtubule arrays are more disorganized and also less dense, presumably because tubulin levels are limiting. Regulation of the prefoldin complex is also important for microtubule-dependent processes in animal cells. In particular, prefoldin expression levels correlate ...
17.2 Notes
17.2 Notes

... half, producing two _________________________________________________ ...
PDF
PDF

... (Suzuki et al. 1990 and our unpublished data). TC-14 might be involved in asexual reproduction of budding tunicates. In this work, we have purified TC-14, labeled a portion of it with fluorescein or biotin and prepared anti-TC-14 polyclonal antibodies. They were used to examine the expression, local ...
Cell adhesion and phagocytosis promoted by monoclonal
Cell adhesion and phagocytosis promoted by monoclonal

The endoplasmic reticulum exerts control over organelle streaming
The endoplasmic reticulum exerts control over organelle streaming

... cytoskeletal forces are in place to propel these organelles. The ER is arguably one of the organelles with the largest membrane extension and it is in close vicinity to the other organelles (Fig. S3). The evidence proposed above that ER streaming does not entirely depend on the cytoskeleton (Fig. 2C ...
Small airway-on-a-chip enables analysis of human lung
Small airway-on-a-chip enables analysis of human lung

... these clinically relevant responses in vitro, we analyzed the effects of exposing the airway epithelium to the viral mimic polyinosinic-polycytidylic acid (poly I:C) (10 µg ml-1). Poly I:C is a synthetic analogue of the double-stranded RNA that is produced in infected cells during viral replication ...
Evidence that the transport of ricin to the cytoplasm is independent
Evidence that the transport of ricin to the cytoplasm is independent

... Cells were transfected with expression plasmids encoding either wildtype Rab6A or mutant Rab6A (Rab6A-T27N) using LipofectAMINE 2000 (Life Technologies, Inc., Rockville, MD, USA) following the protocol provided by the vendor. In brief, cells were plated in 24-well plates at a density of 2×105 cells ...
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review - Nature

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CHAPTER SIX The role of viruses in disease
CHAPTER SIX The role of viruses in disease

... free, thus causing symptoms of disease, again this is a special case caused by injury and not an infectious disease. Viruses on the other hand are not living microbes, they have not been demonstrated as the cause of disease, they are mostly present in stable relationships with certain host cells and ...
The Care and Feeding of the Microscope Lab 5
The Care and Feeding of the Microscope Lab 5

... Magnification is the ratio of an object’s image size to its real size; magnification simply makes the objects or specimens appear larger. Resolution (resolving power) is the ability of an optical instrument to distinguish between two points that are close together, so that they are seen as separate ...
Demonstration by single-cell PCR that Reed–Sternberg cells and
Demonstration by single-cell PCR that Reed–Sternberg cells and

... repeats of the 33 bp motif, whereas the Ta fragment contained only three repeats (the corresponding fragment amplified from the standard B95.8 isolate contains 4n5 repeats of the 33 bp motif ; Baer et al., 1984). Most importantly, sequence differences observed between the two fragments Ta and Tb inv ...
Growth Factors Are Released by Mechanically Wounded
Growth Factors Are Released by Mechanically Wounded

... injured or in undisturbed but motile tissues in vivo might also be wounded at their plasma membranes. However, such membrane wounds would probably not be detected by conventional morphological methods because of the cell's seemingly well-developed ability to reseal and thus survive them. Using novel ...
Selective Expression of a Novel Surface Molecule by Human Th2
Selective Expression of a Novel Surface Molecule by Human Th2

... that were differentially hybridized with the subtracted Th2 (2P26derived) but not Th1 (2P15-derived) probe. Northern blot analyses using total RNAs of 2P15 and 2P26 cells showed that 13 of the 45 cDNA fragments were actually expressed at detectable levels in a 2P26-specific manner. Search of the EMB ...
Origin of Prokaryotes and Eukaryotes
Origin of Prokaryotes and Eukaryotes

... support for the viral origin of the nucleus hypothesis (Philippe et al. 2013). These virus particles are large enough to be visible under light microscope and contain DNA genomes larger in size and gene content than some bacteria and simple eukaryotes (Xiao et al. 2009). Amongst the more than 1,200 ...
Syllabus, BIOSC 2105: Cell Signaling Spring Term, 2014 Instructor
Syllabus, BIOSC 2105: Cell Signaling Spring Term, 2014 Instructor

Cell Transport Power Point
Cell Transport Power Point

... on the inside of the cell than the outside of the cell. • Water will diffuse from high to low causing water to leave the cell • This will cause the cell to shrink. • Plasmolysis: the shrinking of a cell. ...
Plant Cell - Wesleyan College Faculty
Plant Cell - Wesleyan College Faculty

... together into strong sheets. Intermediate filaments made of sturdy keratin proteins anchor desmosomes in the cytoplasm. ...
2.4 Cell Diversity - Science at St. Dominics
2.4 Cell Diversity - Science at St. Dominics

... and to fight infection. ...
THE SEPARATION OF DIFFERENT CELL CLASSES FROM
THE SEPARATION OF DIFFERENT CELL CLASSES FROM

... The lymphocyte fraction appears fully active in its ability to initiate immune responses to at least two different antigens, but is changed in over-all composition and selectively depleted in certain classes of antibody-forming cells . ...
Endocytosis of cigarette-smoke condensate by rabbit alveolar
Endocytosis of cigarette-smoke condensate by rabbit alveolar

... was in contrast to the non-phagocytic cells (lymphocytes) which only showed a slightly enhanced fluorescence at 37°C. These findings suggest that the CSC-uptake is mainly an endocytic process, since low temperature effectively inhibits such mechanisms [22]. On the other hand, an increased fluorescen ...
Cell death in Leishmania induced by stress and differentiation
Cell death in Leishmania induced by stress and differentiation

... degradation have been described.8,12,30 However, none of these reports provide answers about the implicated pathways except in the case of death in Trypanosoma brucei, which was shown to be Ca2+-dependent when induced by reactive oxygen species (ROS). In this case, however, parasites expressing mous ...
Molecular sieving properties of the cytoplasm of Escherichia coli and
Molecular sieving properties of the cytoplasm of Escherichia coli and

... orders of magnitude lower), this may not reveal the reaction conditions present inside living cells (Ellis, 2001). It is thus important to have quantitative information on the equilibria of molecule associations, as well as the diffusion of low and high molecular weight molecules in the cytoplasm of ...
Domains and Kingdoms
Domains and Kingdoms

... last common ancestor of all life on Earth may have been archaea – that’s how old they are! Archaea are Prokaryotic (does not have a nucleus) and unicellular. Archaea are often found in extreme environments where other life cannot survive. For example, these extremophiles can be found in extremely ho ...
cell cycle
cell cycle

... © 2014 Pearson Education, Inc. ...
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Amitosis

Amitosis (a- + mitosis) is absence of mitosis, the usual form of cell division in the cells of eukaryotes. There are several senses in which eukaryotic cells can be amitotic. One refers to capability for non-mitotic division and the other refers to lack of capability for division. In one sense of the word, which is now mostly obsolete, amitosis is cell division in eukaryotic cells that happens without the usual features of mitosis as seen on microscopy, namely, without nuclear envelope breakdown and without formation of mitotic spindle and condensed chromosomes as far as microscopy can detect. However, most examples of cell division formerly thought to belong to this supposedly ""non-mitotic"" class, such as the division of unicellular eukaryotes, are today recognized as belonging to a class of mitosis called closed mitosis. A spectrum of mitotic activity can be categorized as open, semi-closed, and closed mitosis, depending on the fate of the nuclear envelope. An exception is the division of ciliate macronucleus, which is not mitotic, and the reference to this process as amitosis may be the only legitimate use of the ""non-mitotic division"" sense of the term today. In animals and plants which normally have open mitosis, the microscopic picture described in the 19th century as amitosis most likely corresponded to apoptosis, a process of programmed cell death associated with fragmentation of the nucleus and cytoplasm. Relatedly, even in the late 19th century cytologists mentioned that in larger life forms, amitosis is a ""forerunner of degeneration"".Another sense of amitotic refers to cells of certain tissues that are usually no longer capable of mitosis once the organism has matured into adulthood. In humans this is true of various muscle and nerve tissue types; if the existing ones are damaged, they cannot be replaced with new ones of equal capability. For example, cardiac muscle destroyed by heart attack and nerves destroyed by piercing trauma usually cannot regenerate. In contrast, skin cells are capable of mitosis throughout adulthood; old skin cells that die and slough off are replaced with new ones. Human liver tissue also has a sort of dormant regenerative ability; it is usually not needed or expressed but can be elicited if needed.
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