Hijacking of eukaryotic functions by intracellular bacterial pathogens
... Studies using tissue culture cells have shown that InlB is essential for Listeria uptake by most non-phagocytic cell types, such as hepatocytes, endothelial cells, fibroblasts and certain epithelial cell lines. In contrast, InlA-mediated invasion is apparently restricted to cells expressing E-cadher ...
... Studies using tissue culture cells have shown that InlB is essential for Listeria uptake by most non-phagocytic cell types, such as hepatocytes, endothelial cells, fibroblasts and certain epithelial cell lines. In contrast, InlA-mediated invasion is apparently restricted to cells expressing E-cadher ...
Plant Cytoskeleton: DELLA Connects Gibberellins to Microtubules
... these conditions, the cortical microtubule arrays are more disorganized and also less dense, presumably because tubulin levels are limiting. Regulation of the prefoldin complex is also important for microtubule-dependent processes in animal cells. In particular, prefoldin expression levels correlate ...
... these conditions, the cortical microtubule arrays are more disorganized and also less dense, presumably because tubulin levels are limiting. Regulation of the prefoldin complex is also important for microtubule-dependent processes in animal cells. In particular, prefoldin expression levels correlate ...
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... (Suzuki et al. 1990 and our unpublished data). TC-14 might be involved in asexual reproduction of budding tunicates. In this work, we have purified TC-14, labeled a portion of it with fluorescein or biotin and prepared anti-TC-14 polyclonal antibodies. They were used to examine the expression, local ...
... (Suzuki et al. 1990 and our unpublished data). TC-14 might be involved in asexual reproduction of budding tunicates. In this work, we have purified TC-14, labeled a portion of it with fluorescein or biotin and prepared anti-TC-14 polyclonal antibodies. They were used to examine the expression, local ...
The endoplasmic reticulum exerts control over organelle streaming
... cytoskeletal forces are in place to propel these organelles. The ER is arguably one of the organelles with the largest membrane extension and it is in close vicinity to the other organelles (Fig. S3). The evidence proposed above that ER streaming does not entirely depend on the cytoskeleton (Fig. 2C ...
... cytoskeletal forces are in place to propel these organelles. The ER is arguably one of the organelles with the largest membrane extension and it is in close vicinity to the other organelles (Fig. S3). The evidence proposed above that ER streaming does not entirely depend on the cytoskeleton (Fig. 2C ...
Small airway-on-a-chip enables analysis of human lung
... these clinically relevant responses in vitro, we analyzed the effects of exposing the airway epithelium to the viral mimic polyinosinic-polycytidylic acid (poly I:C) (10 µg ml-1). Poly I:C is a synthetic analogue of the double-stranded RNA that is produced in infected cells during viral replication ...
... these clinically relevant responses in vitro, we analyzed the effects of exposing the airway epithelium to the viral mimic polyinosinic-polycytidylic acid (poly I:C) (10 µg ml-1). Poly I:C is a synthetic analogue of the double-stranded RNA that is produced in infected cells during viral replication ...
Evidence that the transport of ricin to the cytoplasm is independent
... Cells were transfected with expression plasmids encoding either wildtype Rab6A or mutant Rab6A (Rab6A-T27N) using LipofectAMINE 2000 (Life Technologies, Inc., Rockville, MD, USA) following the protocol provided by the vendor. In brief, cells were plated in 24-well plates at a density of 2×105 cells ...
... Cells were transfected with expression plasmids encoding either wildtype Rab6A or mutant Rab6A (Rab6A-T27N) using LipofectAMINE 2000 (Life Technologies, Inc., Rockville, MD, USA) following the protocol provided by the vendor. In brief, cells were plated in 24-well plates at a density of 2×105 cells ...
review - Nature
... completely from the route leading from sIg to S phase entry, in that Btk is absolutely required for the latter but not at all for the former. The finding that Fas-resistance develops over a period of hours following anti-Ig treatment suggested that new gene expression might play a key role. To exami ...
... completely from the route leading from sIg to S phase entry, in that Btk is absolutely required for the latter but not at all for the former. The finding that Fas-resistance develops over a period of hours following anti-Ig treatment suggested that new gene expression might play a key role. To exami ...
CHAPTER SIX The role of viruses in disease
... free, thus causing symptoms of disease, again this is a special case caused by injury and not an infectious disease. Viruses on the other hand are not living microbes, they have not been demonstrated as the cause of disease, they are mostly present in stable relationships with certain host cells and ...
... free, thus causing symptoms of disease, again this is a special case caused by injury and not an infectious disease. Viruses on the other hand are not living microbes, they have not been demonstrated as the cause of disease, they are mostly present in stable relationships with certain host cells and ...
The Care and Feeding of the Microscope Lab 5
... Magnification is the ratio of an object’s image size to its real size; magnification simply makes the objects or specimens appear larger. Resolution (resolving power) is the ability of an optical instrument to distinguish between two points that are close together, so that they are seen as separate ...
... Magnification is the ratio of an object’s image size to its real size; magnification simply makes the objects or specimens appear larger. Resolution (resolving power) is the ability of an optical instrument to distinguish between two points that are close together, so that they are seen as separate ...
Demonstration by single-cell PCR that Reed–Sternberg cells and
... repeats of the 33 bp motif, whereas the Ta fragment contained only three repeats (the corresponding fragment amplified from the standard B95.8 isolate contains 4n5 repeats of the 33 bp motif ; Baer et al., 1984). Most importantly, sequence differences observed between the two fragments Ta and Tb inv ...
... repeats of the 33 bp motif, whereas the Ta fragment contained only three repeats (the corresponding fragment amplified from the standard B95.8 isolate contains 4n5 repeats of the 33 bp motif ; Baer et al., 1984). Most importantly, sequence differences observed between the two fragments Ta and Tb inv ...
Growth Factors Are Released by Mechanically Wounded
... injured or in undisturbed but motile tissues in vivo might also be wounded at their plasma membranes. However, such membrane wounds would probably not be detected by conventional morphological methods because of the cell's seemingly well-developed ability to reseal and thus survive them. Using novel ...
... injured or in undisturbed but motile tissues in vivo might also be wounded at their plasma membranes. However, such membrane wounds would probably not be detected by conventional morphological methods because of the cell's seemingly well-developed ability to reseal and thus survive them. Using novel ...
Selective Expression of a Novel Surface Molecule by Human Th2
... that were differentially hybridized with the subtracted Th2 (2P26derived) but not Th1 (2P15-derived) probe. Northern blot analyses using total RNAs of 2P15 and 2P26 cells showed that 13 of the 45 cDNA fragments were actually expressed at detectable levels in a 2P26-specific manner. Search of the EMB ...
... that were differentially hybridized with the subtracted Th2 (2P26derived) but not Th1 (2P15-derived) probe. Northern blot analyses using total RNAs of 2P15 and 2P26 cells showed that 13 of the 45 cDNA fragments were actually expressed at detectable levels in a 2P26-specific manner. Search of the EMB ...
Origin of Prokaryotes and Eukaryotes
... support for the viral origin of the nucleus hypothesis (Philippe et al. 2013). These virus particles are large enough to be visible under light microscope and contain DNA genomes larger in size and gene content than some bacteria and simple eukaryotes (Xiao et al. 2009). Amongst the more than 1,200 ...
... support for the viral origin of the nucleus hypothesis (Philippe et al. 2013). These virus particles are large enough to be visible under light microscope and contain DNA genomes larger in size and gene content than some bacteria and simple eukaryotes (Xiao et al. 2009). Amongst the more than 1,200 ...
Cell Transport Power Point
... on the inside of the cell than the outside of the cell. • Water will diffuse from high to low causing water to leave the cell • This will cause the cell to shrink. • Plasmolysis: the shrinking of a cell. ...
... on the inside of the cell than the outside of the cell. • Water will diffuse from high to low causing water to leave the cell • This will cause the cell to shrink. • Plasmolysis: the shrinking of a cell. ...
Plant Cell - Wesleyan College Faculty
... together into strong sheets. Intermediate filaments made of sturdy keratin proteins anchor desmosomes in the cytoplasm. ...
... together into strong sheets. Intermediate filaments made of sturdy keratin proteins anchor desmosomes in the cytoplasm. ...
THE SEPARATION OF DIFFERENT CELL CLASSES FROM
... The lymphocyte fraction appears fully active in its ability to initiate immune responses to at least two different antigens, but is changed in over-all composition and selectively depleted in certain classes of antibody-forming cells . ...
... The lymphocyte fraction appears fully active in its ability to initiate immune responses to at least two different antigens, but is changed in over-all composition and selectively depleted in certain classes of antibody-forming cells . ...
Endocytosis of cigarette-smoke condensate by rabbit alveolar
... was in contrast to the non-phagocytic cells (lymphocytes) which only showed a slightly enhanced fluorescence at 37°C. These findings suggest that the CSC-uptake is mainly an endocytic process, since low temperature effectively inhibits such mechanisms [22]. On the other hand, an increased fluorescen ...
... was in contrast to the non-phagocytic cells (lymphocytes) which only showed a slightly enhanced fluorescence at 37°C. These findings suggest that the CSC-uptake is mainly an endocytic process, since low temperature effectively inhibits such mechanisms [22]. On the other hand, an increased fluorescen ...
Cell death in Leishmania induced by stress and differentiation
... degradation have been described.8,12,30 However, none of these reports provide answers about the implicated pathways except in the case of death in Trypanosoma brucei, which was shown to be Ca2+-dependent when induced by reactive oxygen species (ROS). In this case, however, parasites expressing mous ...
... degradation have been described.8,12,30 However, none of these reports provide answers about the implicated pathways except in the case of death in Trypanosoma brucei, which was shown to be Ca2+-dependent when induced by reactive oxygen species (ROS). In this case, however, parasites expressing mous ...
Molecular sieving properties of the cytoplasm of Escherichia coli and
... orders of magnitude lower), this may not reveal the reaction conditions present inside living cells (Ellis, 2001). It is thus important to have quantitative information on the equilibria of molecule associations, as well as the diffusion of low and high molecular weight molecules in the cytoplasm of ...
... orders of magnitude lower), this may not reveal the reaction conditions present inside living cells (Ellis, 2001). It is thus important to have quantitative information on the equilibria of molecule associations, as well as the diffusion of low and high molecular weight molecules in the cytoplasm of ...
Domains and Kingdoms
... last common ancestor of all life on Earth may have been archaea – that’s how old they are! Archaea are Prokaryotic (does not have a nucleus) and unicellular. Archaea are often found in extreme environments where other life cannot survive. For example, these extremophiles can be found in extremely ho ...
... last common ancestor of all life on Earth may have been archaea – that’s how old they are! Archaea are Prokaryotic (does not have a nucleus) and unicellular. Archaea are often found in extreme environments where other life cannot survive. For example, these extremophiles can be found in extremely ho ...