Isolation of N-Acetylmuramoyl-L-Alanine Amidase Gene (amiB) from
... and L-amino acids in bacterial cell walls. As shown in Fig. 6A, the Ami domain of the N-terminus is very similar to those of V. cholerae, V. fischeri, V. parahaemolyticus, and V. vulnificus: The identities were 90%, 80%, 75%, and 73%, respectively. Three repeated LysM motifs of the Cterminus found i ...
... and L-amino acids in bacterial cell walls. As shown in Fig. 6A, the Ami domain of the N-terminus is very similar to those of V. cholerae, V. fischeri, V. parahaemolyticus, and V. vulnificus: The identities were 90%, 80%, 75%, and 73%, respectively. Three repeated LysM motifs of the Cterminus found i ...
Primordial germ cells migration: morphological and molecular aspects
... development and migrate by a well-defined route into the genital ridges (Witschi, 1948; Chiqouine, 1954). Since PGCs present high alkaline phosphatase activity, this activity has been used as a marker through which they have been identified in an extra-embryonic region near the yolk sac early in emb ...
... development and migrate by a well-defined route into the genital ridges (Witschi, 1948; Chiqouine, 1954). Since PGCs present high alkaline phosphatase activity, this activity has been used as a marker through which they have been identified in an extra-embryonic region near the yolk sac early in emb ...
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... expressed at higher levels in XY than in XX gonads throughout these stages (see Fig. S1 in the supplementary material). Using both methods, Notch2 was detected at low levels at 11.5 dpc (Fig. 1A,M,P). lacZ-positive cells (detected using an antibody against β-gal) were found in the coelomic epitheliu ...
... expressed at higher levels in XY than in XX gonads throughout these stages (see Fig. S1 in the supplementary material). Using both methods, Notch2 was detected at low levels at 11.5 dpc (Fig. 1A,M,P). lacZ-positive cells (detected using an antibody against β-gal) were found in the coelomic epitheliu ...
IMPACT SAMR Cover Sheet
... notes from a power point presentation on the cell. Use technology when appropriate to enhance laboratory investigations and presentations of findings. (SI-H-A3) ...
... notes from a power point presentation on the cell. Use technology when appropriate to enhance laboratory investigations and presentations of findings. (SI-H-A3) ...
Tumor Specific Antigens
... that appear in patients with cancer and cannot be readily explained either by the local or distal spread of the tumor or by the elaboration of hormones indigenous to the tissue of origin of the tumor . Occur in 10-15% of tumors ...
... that appear in patients with cancer and cannot be readily explained either by the local or distal spread of the tumor or by the elaboration of hormones indigenous to the tissue of origin of the tumor . Occur in 10-15% of tumors ...
Essential Medical Microbiology
... the absence of oxygen (e.g., Clostridium tetani,). oxygen is lethal to these organisms. ...
... the absence of oxygen (e.g., Clostridium tetani,). oxygen is lethal to these organisms. ...
microbe images
... surfaces of other bacteria, host cells and some are specialised for DNA transfer. ...
... surfaces of other bacteria, host cells and some are specialised for DNA transfer. ...
Extracellular Matrix Components Regulate Cellular Polarity and
... CNS) implies that the basement membrane organization provides crucial guidance during retinal development. Ectodermal formation and epithelial development is critically dependent on the laminin-rich basement membrane, which confers polarity cues, regulates proliferation and provides a substrate for ...
... CNS) implies that the basement membrane organization provides crucial guidance during retinal development. Ectodermal formation and epithelial development is critically dependent on the laminin-rich basement membrane, which confers polarity cues, regulates proliferation and provides a substrate for ...
KRP3A and KRP3B: Candidate Motors in Spermatid Maturation in
... tasks ranging from chromosome segregation to vesicle transport (reviewed in [1–3]). The cells of the seminiferous epithelium provide an excellent opportunity to examine the different structural demands on these specialized proteins in an array of microtubule complexes. Although microtubule-based mov ...
... tasks ranging from chromosome segregation to vesicle transport (reviewed in [1–3]). The cells of the seminiferous epithelium provide an excellent opportunity to examine the different structural demands on these specialized proteins in an array of microtubule complexes. Although microtubule-based mov ...
Most Human Proteins Made in Both Nucleus
... images). After 30 s in puromycin, the nuclear signal decreases and becomes more diffuse (Fig. 2C). After 60 s, the peri-nuclear region becomes the brightest (Fig. 2D,E). These changes mimic those seen previously and are simply interpreted in light of the known behaviour of puromycylated peptides: af ...
... images). After 30 s in puromycin, the nuclear signal decreases and becomes more diffuse (Fig. 2C). After 60 s, the peri-nuclear region becomes the brightest (Fig. 2D,E). These changes mimic those seen previously and are simply interpreted in light of the known behaviour of puromycylated peptides: af ...
Nuclear Factor of Activated T Cells - JEM
... for the role of CBP and p300 in tumorigenesis (9). Specifically, certain cases of acute myeloid leukemia have been linked to recurrent chromosomal translocations that result in in frame fusions of CBP or p300 to the monocytic leukemia zinc finger protein (12) and myeloid/lymphoid leukemia (13, 14) g ...
... for the role of CBP and p300 in tumorigenesis (9). Specifically, certain cases of acute myeloid leukemia have been linked to recurrent chromosomal translocations that result in in frame fusions of CBP or p300 to the monocytic leukemia zinc finger protein (12) and myeloid/lymphoid leukemia (13, 14) g ...
Making a firm decision: multifaceted regulation of cell fate in the
... The first fate decision in the mouse embryo is taken as two populations of cells are set apart. Cells positioned inside (the ICM) retain pluripotency and cells on the outside develop into extraembryonic trophectoderm. This first set of extraembryonic cells will support the development of the embryo ...
... The first fate decision in the mouse embryo is taken as two populations of cells are set apart. Cells positioned inside (the ICM) retain pluripotency and cells on the outside develop into extraembryonic trophectoderm. This first set of extraembryonic cells will support the development of the embryo ...
CELL DIVISION AND DNA SYNTHESIS IN TETRAHYMENA
... at which D N A synthesis begins is also demonstrated by the change in the utilization of H 3thymidine of the medium for macronuclear D N A synthesis. Amino acid deprivation before D N A synthesis begins prevents the subsequent uptake of H3-thymidine into macronuclear D N A although macronuclear D N ...
... at which D N A synthesis begins is also demonstrated by the change in the utilization of H 3thymidine of the medium for macronuclear D N A synthesis. Amino acid deprivation before D N A synthesis begins prevents the subsequent uptake of H3-thymidine into macronuclear D N A although macronuclear D N ...
PULP
... the neural receptor in the pulp, the central process of a trigeminal sensory neuron traverses the trigeminal ganglion located in the floor of the middle ...
... the neural receptor in the pulp, the central process of a trigeminal sensory neuron traverses the trigeminal ganglion located in the floor of the middle ...
Beyond ergosterol
... Another critical consequence of disabling V-ATPase is inhibition of filamentous growth, as was observed for the vma7-/- strain of C. albicans in the presence of serum or liquid Spider medium (with mannitol).26 Fluconazole treatment and ERG3 deletion have both been shown to inhibit filamentation,28, ...
... Another critical consequence of disabling V-ATPase is inhibition of filamentous growth, as was observed for the vma7-/- strain of C. albicans in the presence of serum or liquid Spider medium (with mannitol).26 Fluconazole treatment and ERG3 deletion have both been shown to inhibit filamentation,28, ...
HYPERTROPHY OF THE HUMAN HEART AT THE LEVEL OF FINE
... Muscle cells in the left ventricular walls of four markedly hypertrophied human hearts (above 600 gm) were compared with muscle cells in four non-hypertrophied hearts (up to 310 gm). Blocks of tissue obtained postmortem within 6 hours were processed for light and electron microscopy under conditions ...
... Muscle cells in the left ventricular walls of four markedly hypertrophied human hearts (above 600 gm) were compared with muscle cells in four non-hypertrophied hearts (up to 310 gm). Blocks of tissue obtained postmortem within 6 hours were processed for light and electron microscopy under conditions ...
Mechanism of Inhibition of Cell Proliferation by
... alkaloids are concentrated intracellularly (14, 15) and accumu late differentially in some cells (14). Alternatively, one or all of the Vinca alkaloids may act on targets other than tubulin or microtubules. The Vinca alkaloids are known to affect several cellular processes that appear unrelated to m ...
... alkaloids are concentrated intracellularly (14, 15) and accumu late differentially in some cells (14). Alternatively, one or all of the Vinca alkaloids may act on targets other than tubulin or microtubules. The Vinca alkaloids are known to affect several cellular processes that appear unrelated to m ...
Flow Cytometry and Sorting, Part 1
... Need to have cells in suspension flow in single file through an illuminated volume In most instruments, accomplished by injecting sample into a sheath fluid as it passes through a small (50-300 µm) ...
... Need to have cells in suspension flow in single file through an illuminated volume In most instruments, accomplished by injecting sample into a sheath fluid as it passes through a small (50-300 µm) ...
FREEMAN MEDIA INTEGRATION GUIDE Chapter 7: Inside the Cell
... Localization Signal Experiment: Experimental demonstration of a nuclear localization signal on polypeptides transported into the nucleus. Nuclear, cytoplasmic, and hybrid proteins are followed after injection into the cell cytoplasm. Run time: 1:15 •A Pulse-Chase Experiment: Instructor version of st ...
... Localization Signal Experiment: Experimental demonstration of a nuclear localization signal on polypeptides transported into the nucleus. Nuclear, cytoplasmic, and hybrid proteins are followed after injection into the cell cytoplasm. Run time: 1:15 •A Pulse-Chase Experiment: Instructor version of st ...
Achieving Desired Product Profile Read More
... Expedited process and product characterization at early development stage can reduce clinical risk by ensuring high quality of the product. In contrast, template processes, although seemingly faster, may yield ...
... Expedited process and product characterization at early development stage can reduce clinical risk by ensuring high quality of the product. In contrast, template processes, although seemingly faster, may yield ...
Cell Surface Membrane Remodelling and Mitochondrial
... To reduce complications associated with cell overlap, PC12 cells and primary neurons are plated at low density (5×104 cells/ 35 mm dish). Undifferentiated PC12 cells are treated with NGF for 4-6 days to induce differentiation or can be are differentiated in tissue culture flasks for up to 10 days pr ...
... To reduce complications associated with cell overlap, PC12 cells and primary neurons are plated at low density (5×104 cells/ 35 mm dish). Undifferentiated PC12 cells are treated with NGF for 4-6 days to induce differentiation or can be are differentiated in tissue culture flasks for up to 10 days pr ...