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Plasmids in Enterobacteriaceae - EURL-AR
Plasmids in Enterobacteriaceae - EURL-AR

... Carattoli,A., Bertini,A., Villa,L., Falbo,V., Hopkins,K.L., Threlfall,E.J., 2005. Identification of plasmids by PCR-based replicon typing. J. Microbiol. Methods, 63, 219-228. Garcillan-Barcia,M.P., Alvarado,A., de la,C.F., 2011. Identification of bacterial plasmids based on mobility and plasmid popu ...
Mitosis Meiosis Notes
Mitosis Meiosis Notes

... 2. Example: Fragile X syndrome a. twice as many males as females have this. b. there is a region on the X chromosome that has many replications which elongates the X chromosome. Family history of Fragile X syndrome, especially a male relative Mental retardation Large testicles after puberty Large bo ...
The Florida State University College of Arts and Sciences
The Florida State University College of Arts and Sciences

... silencing can be induced by double stranded RNAs (dsRNA) with sequences that are homologous to the promoter region (Mette et al. 2000). The pathway responsible for this epigenetic transcriptional gene silencing is known as the RNA-dependent DNA methylation pathway or RdDM. RdDM relies on the coordin ...
adrian2004_1acbPosterDataAnalysis34by51
adrian2004_1acbPosterDataAnalysis34by51

... patients. A web form is used to retrieve microarray data form the DORA database. In the resulting Excel file (Figure 1), each column holds gene expression data for a patient and each row holds gene expression data for a gene. Each column is headed by a patient ID. Each row is headed by a gene title ...
Caenibacterium thermophilum is a later synonym of Schlegelella
Caenibacterium thermophilum is a later synonym of Schlegelella

... from a thermophilic municipal sludge digester, was investigated at the same time, in parallel, in a different laboratory (Manaia et al., 2003). Therefore, Caenibacterium thermophilum and S. thermodepolymerans could be compared phylogenetically only after the published data became available (presente ...
You Light Up My Life
You Light Up My Life

...  Only mutations that arise in germ cells can be passed on to next generation ...
Gene Section IGH (Immunoglobulin Heavy) Atlas of Genetics and Cytogenetics
Gene Section IGH (Immunoglobulin Heavy) Atlas of Genetics and Cytogenetics

BACULOVIRUS MEDIATED PRODUCTION OF INFECTIOUS
BACULOVIRUS MEDIATED PRODUCTION OF INFECTIOUS

... (2). Similar to previous reports, serious losses caused by IMNV has been known to be due to the cumulative mortalities in affected ponds which could reach 70% (3). In this study, we cloned the full-length IMNV genome into a baculovirus transfer vector under the control of the polyhedrin promoter. To ...
23_InstGuide_AR
23_InstGuide_AR

... Many students find it hard to understand the HardyWeinberg theorem and do not know how and when to use the Hardy-Weinberg equations. They do not realize that the Hardy-Weinberg theorem clarifies the factors that alter allele frequency, and that it does not imply that allele frequencies are static. T ...
Document
Document

... 3. Compare the % of parental to recombinants A. If 50% parental:50% recombinant – Independent Assortment B. If more parental than recombinant – partial linkage C. If only parental and no recombinant – complete linkage ...
Gender, Genes and Genetics: From Darwin to the Human Genome
Gender, Genes and Genetics: From Darwin to the Human Genome

... by a step-parent than a child with two biological parents (Daly and Wilson, 1988). It would not explain why an overwhelmingly greater number of human parents willingly adopt children, and most typically display remarkable love and concern for them. It would not explain why midwives in both the USA a ...
1. Who is called the “Father of Genetics”? 2. The different
1. Who is called the “Father of Genetics”? 2. The different

... 24. A ______________ trait (like A, B, and O blood type) is controlled by three or more alleles for the same gene. 25. A characteristic that can be observed such as hair color, seed shape, or flower color is called a ______________ ...
Partial Linkage
Partial Linkage

... 3. Compare the % of parental to recombinants A. If 50% parental:50% recombinant – Independent Assortment B. If more parental than recombinant – partial linkage C. If only parental and no recombinant – complete linkage ...
appENDIX I - VU Research Portal
appENDIX I - VU Research Portal

... group are high-risk haplotypes. For example, in a schizophrenia family based study it was found that a certain combination of 8 allele calls in the DTNBP1 (dystrobrevin binding protein 1) gene were unique for the disease group (Van den Oord et al., 2003). Human individuals differ from one another by ...
1 - Humble ISD
1 - Humble ISD

... Part I – Vocabulary (Choices listed on the next page.) ______ 1. Both alleles show in heterozygote ______ 2. Autosomal recessive disorder characterized by a lack of melanin production ______ 3. Results from change in DNA; may be harmful, beneficial, or silent ______ 4. 47 XXY ______ 5. Multiple phen ...
−Table of Contents
−Table of Contents

... significantly larger than the expected band of 0.5 kb. This could be explained by the dahlia gene having larger introns than the petunia one. Primer pair two resulted in the amplification of two fragment lengths. There are several possible explanations for this. The first is that dahlia could have t ...
Human microRNA target analysis and gene ontology clustering by
Human microRNA target analysis and gene ontology clustering by

... The size of this primary product varies from 100- to 1000nucleotides in length. Then, the pri-miRNA is truncated by Drosha and DGCR8 to form a hairpin loop precursor called pre-miRNA [3]. The 60–70 nucleotide long premiRNA is loaded to Exportin 8 and Ran-GTP in order to be exported into the cytoplas ...
Evolution of the defensin-like gene family in grass genomes
Evolution of the defensin-like gene family in grass genomes

... Gramineae, including brachypodium, rice, maize and sorghum were identified based on bioinformatics methods. Using the synteny analysis method, we found that 21 DEFL genes formed 30 pairs of duplicated blocks that have undergone large-scale duplication events, mostly occurring between species. In part ...
DNA, RNA, and the Flow of Genetic Information
DNA, RNA, and the Flow of Genetic Information

... The building blocks of nucleic acids and the precursors of these building blocks play many other roles throughout the cell—for instance, as energy currency and as molecular signals. Consequently, it is important to be familiar with the nomenclature of nucleotides and their precursors. A unit consist ...
CHARGE sYNDRoME
CHARGE sYNDRoME

... “minor” features of the disorder, which are shown in Table 1. Either all 4 of the major features or 3 major and 3 minor features must be present in order to confirm a diagnosis of CHARGE syndrome.1,2 Because the features of CHARGE syndrome overlap with some other genetic disorders1, your doctor may ...
Signals of recent positive selection in a worldwide sample of human
Signals of recent positive selection in a worldwide sample of human

TAGS: a tool for gene set analysis of expression time series
TAGS: a tool for gene set analysis of expression time series

... corresponding ranks are calculated with the same strategy as above. Finally, gene set analysis is done to find the significant gene sets. The running time depends on the number of candidate sets and, more importantly, the permutation times. A result dialog will open automatically when calculation is ...
Scanning Life`s Matrix: Genes, Proteins, and Small Molecules (2002
Scanning Life`s Matrix: Genes, Proteins, and Small Molecules (2002

... led Mendel to guess, to hypothesize, on the basis of nothing he could see, but just what he could think with his mind, that there must be discreet factors of inheritance. There must be a green form and a yellow form, which, when they come together, the yellow form's not visible in that first generat ...
PDF - European Journal of Biotechnology and Bioscience
PDF - European Journal of Biotechnology and Bioscience

... Difficult to control the exact amount of viral product and the duration of expression (may be possible to bypass this using encapsulated ex-vivo genetically modified cells or selfinactivating cassettes [13] ...
Lab 10: part a
Lab 10: part a

... 2. Select a shoot from your Tobacco Transformation Experiment. Cut a thin section of leaf or stem and place it into an individual well - keep track of where sections come from. 3. Stain sections for 4-5 hr. at 37 in the incubator. 4. After staining, clear or fix the tissue by adding 50µL of 95% eth ...
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Site-specific recombinase technology



Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse
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