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Selective breeding
Selective breeding

... Syringes and projectiles have been used to insert the genes, but they often damage the cells, and often the inserted genes either only work for a short time or don’t work at all. Thursday, May 4, 2017 ...
Distinguishing Among Evolutionary Models for the Maintenance of
Distinguishing Among Evolutionary Models for the Maintenance of

... between these nonallelic homologous sequences can result in the duplication of the intervening sequences, which can then lead in turn to more duplications because of pairing between the new paralogs (Bailey et al. 2003). But other studies in humans have also found multiple cases with no repetitive D ...
The Compound-Heterozygous Filter
The Compound-Heterozygous Filter

... heterozygotes is that one has to figure out whether the two heterozygous variants affect different copies of a gene or the same copy. Usually, that cannot be determined from a single DNA sequence, when the read length is smaller than the distance between the variants. However, when exome data of mor ...
Depat.Anat. Genetic/Lec4 Dr.sarab H. Linkage
Depat.Anat. Genetic/Lec4 Dr.sarab H. Linkage

... are localized in the nonhomologous sections of X-chromosome, and that have no corresponding allele in Y chromosome. The X-linked genes are commonly known as sex linked genes. * X-linked alleles are designated as superscripts to X chromosome. * Heterozygous females are carriers; they do not show the ...
Genetics Workbook
Genetics Workbook

... is the probability of producing an F2 individual that is phenotypically identical to the P individual on the left? What is the probability of producing an F2 individual that has a genotype identical to the P individual on the right? 5. What is the function of the enzyme aminoacyl tRNA synthetase? Ho ...
Inheritance Intro
Inheritance Intro

... DNA molecules carry the code that controls what your cells are made of and what they do. ...
Cell division and inheritance (Student Support)
Cell division and inheritance (Student Support)

... were all either tall or dwarf. He started off by taking plants which was pure-breeding for tallness( this means when bred with itself or other tall plants they only produced tall plants)and plants which were pure-breeding for dwarfness (this means when bred with itself or others dwarf plants they on ...
DNA technologies
DNA technologies

... expressed within a tissue or organism the collective cDNA clones when placed in a suitable plasmid are called a "cDNA library." This cDNA library should contain the entire repertoire of proteins being used by the host cells just before mRNA was collected. These "libraries" can then be used to isolat ...
Pre-implantation genetic diagnosis FAQs
Pre-implantation genetic diagnosis FAQs

... Charlie Whitaker suffered from Diamond Blackfan Anaemia (DBA) The best possible chance for Charlie to live a relatively normal life was to perform a transplant from a tissue-matched donor The family wished to screen their embryos to select a tissue match for Charlie. At the time, however the HFEA wo ...
Name
Name

... - Mendel thought (incorrectly) that it coded for a specific trait. This definition is OK, but it doesn't reflect what we now know about genetics. Allele: - These are alternate forms of the same gene created by mutations in the genetic code. Some genes have multiple alleles, such as blood type (three ...
hereditary hearing loss
hereditary hearing loss

... both. Conductive hearing loss results from abnormalities of the external ear and/or the ossicles of the middle ear. SNHL results from malfunction of inner ear structures. Hearing loss can be either syndromic, having malformations of the external ear or other organs and/or medical problems involving ...
17_Lecture_Presentation
17_Lecture_Presentation

...  Two structural features of eukaryotes distinguish them from prokaryotes – Eukaryotic genes are situated on chromosomes that occupy a distinct location – Eukaryotic DNA is combined with histones and nonhistone proteins to form chromatin – Compact chromatin structure inhibits transcription, replicat ...
Rate Asymmetry After Genome Duplication Causes Substantial
Rate Asymmetry After Genome Duplication Causes Substantial

... However, a previous study of gene pairs formed by WGD in the yeast Saccharomyces cerevisiae found conflicting topologies among neighbor-joining (NJ) trees drawn from different loci and suggested that this conflict was the result of ‘‘asynchronous functional divergence’’ of duplicated genes (Langkjae ...
The Role of Oocyte‐Secreted Factors GDF9 and BMP15 in Follicular
The Role of Oocyte‐Secreted Factors GDF9 and BMP15 in Follicular

... and Shimasaki 2002). Unlike GDF9, BMP15 protein is found in the pituitary gland, testis and in several other tissues from many species, suggesting that BMP15 activity is not exclusive to the ovary (Otsuka and Shimasaki 2002). BMP15 mRNA was not detected in oocytes of primordial stage follicles and i ...
Title - Iowa State University
Title - Iowa State University

... a) Both are present in pairs in diploid cells b) Homologous chromosomes separate and factors segregate during meiosis c) Fertilization restores the paired condition of both factors and chromosomes 2. The ___________ theory of ___________ was developed by ___________. a) Hereditary, chromosomes, Sutt ...
Reassignment of the Human CSFl Gene to Chromosome lp13-p21
Reassignment of the Human CSFl Gene to Chromosome lp13-p21

... multiple CSF-1 messenger RNA (mRNA) species with different coding and 3' untranslated sequences.'-" Shortly after human CSFl cDNA and genomic clones were isolated: the gene was mapped by isotopic in situ chromosomal hybridization, together with somatic cell hybrid panels, to chromosome 5 at band q33 ...
TechniquesPresentationQuestion
TechniquesPresentationQuestion

High Efficiency Transient Expression System for
High Efficiency Transient Expression System for

Elegantní dopis
Elegantní dopis

... Frankly speaking, I was glad to read a thesis which has not been created by a mere putting together a bunch of already published papers. Among advantages of such an approach is that the author may use as many figures as he considers necessary/useful and also he/she may explain employed methods in su ...
The wbbD gene of E. coli strain VW187
The wbbD gene of E. coli strain VW187

... encoded by pCM227 in DH5α, was solubilized in 0.1% TritonX100, and purified on glutathione-Sepharose. Coomassie blue stains of the fusion protein separated by SDS–polyacrylamide gel electrophoresis (PAGE) showed a major band at 57 kDa, suggesting a high level of expression. Surprisingly, however, th ...
CH 13 NOTES – Meiosis
CH 13 NOTES – Meiosis

... ● For humans, the haploid number is 23 (n = 23) ● Each set of 23 consists of ● In an unfertilized egg (ovum), the ● In a sperm cell, the sex chromosome may be either X or Y ● the ...
Bioportal_2010
Bioportal_2010

... ftp://ftp.ncbi.nih.gov/blast/executables/release/2.2.24/ ...
Annotation Instruction Sheet A. Information for Beginning Annotators
Annotation Instruction Sheet A. Information for Beginning Annotators

... you will want to consider as you try and determine the best gene model, splice site prediction programs and ab initio gene finders. Splice site prediction program. The “Predicted Splice Sites” tracks on the genome browser show the results of the splice site prediction program GeneSplicer. The output ...
Chapter 22. Nucleic Acids
Chapter 22. Nucleic Acids

... of strands of repeating nucleotides joined in chainlike fashion, but the strands are single and it has the nucleotide uracil (U) where DNA has ...
Your Genes, Your Choices
Your Genes, Your Choices

... is not albino. If it is, he and his wife could choose to have an abortion and try again. What do you think of this choice? Adoption is another choice Martin and his wife could make, instead of risking bearing children who are albino. With adoption, the children would not be their own, genetically. B ...
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Site-specific recombinase technology



Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse
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