Solving the shugoshin puzzle
... Divergent gene orientation is highly conserved We calculated the fraction of adjacent gene pairs that are conserved in orientation between every possible pair of species and plotted it against the species’ divergence times (Figure 1a; see Supplementary Material 1). At large evolutionary distances of ...
... Divergent gene orientation is highly conserved We calculated the fraction of adjacent gene pairs that are conserved in orientation between every possible pair of species and plotted it against the species’ divergence times (Figure 1a; see Supplementary Material 1). At large evolutionary distances of ...
A Mutation in the Eta Subunit of Pyruvate Dehydrogenase
... In previous studies, biochemical and immunologic experiments demonstrated that the proband had variable PD C activity in cells and tissues and that the level of activity corresponded to the amo un t of E] subunit proteins detected by immunoblot analysis (18). Whereas fibroblasts had normal activity ...
... In previous studies, biochemical and immunologic experiments demonstrated that the proband had variable PD C activity in cells and tissues and that the level of activity corresponded to the amo un t of E] subunit proteins detected by immunoblot analysis (18). Whereas fibroblasts had normal activity ...
Microbiology of diabetic foot infections: from Louis Pasteur to Łcrime
... PCR This is a molecular method to amplify a genomic region of interest. When followed by DNA sequencing, the abundance and genetic composition of a gene of interest can be determined. The small subunit (SSU) ribosomal RNA (rRNA) gene in bacteria, called 16S rRNA, is a useful gene target given that i ...
... PCR This is a molecular method to amplify a genomic region of interest. When followed by DNA sequencing, the abundance and genetic composition of a gene of interest can be determined. The small subunit (SSU) ribosomal RNA (rRNA) gene in bacteria, called 16S rRNA, is a useful gene target given that i ...
I. Līduma, T. Tračevska, U. Bērs, A. Žileviča. Phenotypic and Genetic
... epidermidis is their capability to form a biofilm on the surfaces of implanted medical devices. The accumulative phase of biofilm formation is linked to the production of intercellular adhesin encoded by the icaADBC operon and accumulation-associated protein by the aap gene. The aim of the study was ...
... epidermidis is their capability to form a biofilm on the surfaces of implanted medical devices. The accumulative phase of biofilm formation is linked to the production of intercellular adhesin encoded by the icaADBC operon and accumulation-associated protein by the aap gene. The aim of the study was ...
bacterial plasmids - Acta Medica Medianae
... While Gram-negative enterobacteriae usually contain one large plasmid for multiple-drug resistance (20), some Gram-positive bacteria, as staphylococcus, often have resistance genes arranged on several smaller plasmids. The instability of these small plasmids obviously leads to different types of ant ...
... While Gram-negative enterobacteriae usually contain one large plasmid for multiple-drug resistance (20), some Gram-positive bacteria, as staphylococcus, often have resistance genes arranged on several smaller plasmids. The instability of these small plasmids obviously leads to different types of ant ...
Fate maps and the morphogenetic movements of gastrulation
... A similar argument can be made for the formation of the ventral furrow. Some ventralized embryos still have residual dorsoventral polarity such that they still form a ventral invagination although all cells express ventral genes. Since these embryos contain no cells with lateral or dorsal fates, one ...
... A similar argument can be made for the formation of the ventral furrow. Some ventralized embryos still have residual dorsoventral polarity such that they still form a ventral invagination although all cells express ventral genes. Since these embryos contain no cells with lateral or dorsal fates, one ...
Genetic Approaches to Studying Genome Function
... mutation rescues or restores or repairs, in whole or in part, the defect caused by the first mutation. Examples: A nonsense mutation in a gene can be suppressed by a mutation in a tRNA gene in which the anticodon has been mutated to read the nonsense codon (informational suppressor). A temperature-s ...
... mutation rescues or restores or repairs, in whole or in part, the defect caused by the first mutation. Examples: A nonsense mutation in a gene can be suppressed by a mutation in a tRNA gene in which the anticodon has been mutated to read the nonsense codon (informational suppressor). A temperature-s ...
BPA leaflet testing and inheritance
... porphyria is known, an attack can be treated quickly. Delays in treatment can lead to paralysis and sometimes death. Familial PCT (the type caused by a porphyria gene) is also dominant with the gene affecting about 1 in 5 of those who have it. However, many experts think genetic testing is not usefu ...
... porphyria is known, an attack can be treated quickly. Delays in treatment can lead to paralysis and sometimes death. Familial PCT (the type caused by a porphyria gene) is also dominant with the gene affecting about 1 in 5 of those who have it. However, many experts think genetic testing is not usefu ...
Lab Module 10 - philipdarrenjones.com
... locations where the non-sister chromatids physically cross each other. This process is called crossing-over. ...
... locations where the non-sister chromatids physically cross each other. This process is called crossing-over. ...
Evolution: The evolvability enigma
... rarely, these will not be very effective in sexual species in spreading alleles that create enhanced mutation rates, as the advantageous mutation will rapidly come into linkage equilibrium with the high mutation rate allele and the latter will receive no benefit from the former. Partridge and Barton ...
... rarely, these will not be very effective in sexual species in spreading alleles that create enhanced mutation rates, as the advantageous mutation will rapidly come into linkage equilibrium with the high mutation rate allele and the latter will receive no benefit from the former. Partridge and Barton ...
Molecular function - SGD-Wiki - Saccharomyces Genome Database
... sequence features histone modifications, nucleosome org. transcription start sites, 5’ and 3’ UTRs ...
... sequence features histone modifications, nucleosome org. transcription start sites, 5’ and 3’ UTRs ...
Using haplotypes to unravel the inheritance of Holstein coat color
... University of Göttingen. Although it is not the casual condition itself, it is putatively linked to a regulatory mutation with the Black/Red phenotype. This test will be part of next chip from GeneSeek. Therefore, we now have two diagnostic tests (ED and e) to detect changes in a coding region; a li ...
... University of Göttingen. Although it is not the casual condition itself, it is putatively linked to a regulatory mutation with the Black/Red phenotype. This test will be part of next chip from GeneSeek. Therefore, we now have two diagnostic tests (ED and e) to detect changes in a coding region; a li ...
1 Meiotic sex chromosome inactivation is disrupted in
... testis cDNA. Efficiencies of 95-105% were obtained for all primers. For experimental assays, 16µl reactions with a final concentration of 300nM of each primer were run in 96well plates for 40 cycles with annealing at 60°C. We analyzed 3-5 (FACS cells) or three (testes) biological replicates per geno ...
... testis cDNA. Efficiencies of 95-105% were obtained for all primers. For experimental assays, 16µl reactions with a final concentration of 300nM of each primer were run in 96well plates for 40 cycles with annealing at 60°C. We analyzed 3-5 (FACS cells) or three (testes) biological replicates per geno ...
Uria_et_al_2006 _ADH paper
... AKR superfamily is divided into 14 families, named AKR1-AKR14, found in both prokaryotes and eukaryotes (Hyndman, et al., 2003). The majority of mammalian enzymes belongs to the AKR1 family, but there is diversity between the microbial enzymes, which are distributed in 10 families: AKR2 (xylose redu ...
... AKR superfamily is divided into 14 families, named AKR1-AKR14, found in both prokaryotes and eukaryotes (Hyndman, et al., 2003). The majority of mammalian enzymes belongs to the AKR1 family, but there is diversity between the microbial enzymes, which are distributed in 10 families: AKR2 (xylose redu ...
A familial inverted duplication/deletion of 2p25.1–25.3
... cut-out of the normal and abnormal chromosomes 2 in G-banding at a resolution of 550 bands. Ideogram of normal and inverted duplicated chromosomes 2p: th 2p25.3 and 2p25.1 bands are depicted in green and red, respectively. The dots represent BAC clones RP11-90H11 (2p25.3, green dot) and RP111B18 (2p ...
... cut-out of the normal and abnormal chromosomes 2 in G-banding at a resolution of 550 bands. Ideogram of normal and inverted duplicated chromosomes 2p: th 2p25.3 and 2p25.1 bands are depicted in green and red, respectively. The dots represent BAC clones RP11-90H11 (2p25.3, green dot) and RP111B18 (2p ...
Chapter 13
... DNA replication starts when a large protein complex (pre-replication complex) binds to a region called origin of replication (ori). ...
... DNA replication starts when a large protein complex (pre-replication complex) binds to a region called origin of replication (ori). ...
The molecular evolution of development
... the HOM/Hox genes in the arthropod Drosophila melanogaster, the vertebrates Homo sapiens and Mus musculus, the cephalochordate Amphioxus and the nematode Caenorhabditis elegans (Fig. 1), Zhang and Nei(24) suggest that extant members of the HOM/Hox gene family were derived not from three precursors, ...
... the HOM/Hox genes in the arthropod Drosophila melanogaster, the vertebrates Homo sapiens and Mus musculus, the cephalochordate Amphioxus and the nematode Caenorhabditis elegans (Fig. 1), Zhang and Nei(24) suggest that extant members of the HOM/Hox gene family were derived not from three precursors, ...
Gene expression profiles in rice gametes and zygotes: identification
... GCS1/HAP2 was identified as a key male membrane protein with a single transmembrane domain and a histidinerich domain in the extracellular region. Sperm cells with a gcs mutation remained attached to the egg cell without cell fusion (Mori et al., 2006). Although the mechanism by which GCS1/HAP2 acts ...
... GCS1/HAP2 was identified as a key male membrane protein with a single transmembrane domain and a histidinerich domain in the extracellular region. Sperm cells with a gcs mutation remained attached to the egg cell without cell fusion (Mori et al., 2006). Although the mechanism by which GCS1/HAP2 acts ...
Genetics Protocol
... disease. Sickle cell anemia, muscular dystrophy, cystic fibrosis are each caused by specific alleles of a human gene, and can therefore be inherited from one generation to the next. Human geneticists illustrate the inheritance of a gene within a family by using a pedigree chart. On such a chart, mal ...
... disease. Sickle cell anemia, muscular dystrophy, cystic fibrosis are each caused by specific alleles of a human gene, and can therefore be inherited from one generation to the next. Human geneticists illustrate the inheritance of a gene within a family by using a pedigree chart. On such a chart, mal ...
Molecular genetics of macular dystrophies
... short arm of chromosome 1 in eight families.' This result is consistent with genetic homogeneity and suggests that possibly only one gene is involved in this form of the disorder. In contrast, several different genes have been implicated in the autosomal dominant form of the disease. Of families stu ...
... short arm of chromosome 1 in eight families.' This result is consistent with genetic homogeneity and suggests that possibly only one gene is involved in this form of the disorder. In contrast, several different genes have been implicated in the autosomal dominant form of the disease. Of families stu ...
Identification and Microsatellite Markers of a Resistance Gene to
... is a complex locus, and composed of 4, 10, 2, and 5 alleles, respectively (Hsam et al., 1998; Zeller et al., 1998; Huang et al., 2003). In our study, we located the gene PmDR147 on the distal region of long arm of chromosome 2A. Powdery mildew resistance genes Pm4a and Pm4b are also located on chrom ...
... is a complex locus, and composed of 4, 10, 2, and 5 alleles, respectively (Hsam et al., 1998; Zeller et al., 1998; Huang et al., 2003). In our study, we located the gene PmDR147 on the distal region of long arm of chromosome 2A. Powdery mildew resistance genes Pm4a and Pm4b are also located on chrom ...
F 1
... In hemophilia, the mutant gene for factor VIII, the clotting factor, is carried on the X chromosome. The affected males inherited their single X chromosome from their mothers—if the mutated form of the gene was present, they would develop the disease. Daughters would inherit a normal X chromosome as ...
... In hemophilia, the mutant gene for factor VIII, the clotting factor, is carried on the X chromosome. The affected males inherited their single X chromosome from their mothers—if the mutated form of the gene was present, they would develop the disease. Daughters would inherit a normal X chromosome as ...
rules - Wiley
... as the segregation of alleles. For each parent, the chance of a gamete with A is 1 in 2 and the chance of a gamete with a is also 1 in 2. These probabilities can also be incorporated into a Punnett square (figure 16.5). The AA and the Aa genotypes both result in normal pigmentation. The aa genotype ...
... as the segregation of alleles. For each parent, the chance of a gamete with A is 1 in 2 and the chance of a gamete with a is also 1 in 2. These probabilities can also be incorporated into a Punnett square (figure 16.5). The AA and the Aa genotypes both result in normal pigmentation. The aa genotype ...
Name
... the same complement of genes with one member of the pair inherited from the father and the other from the mother. Chromosomes that are homologous are almost always the same size, have their centromeres in the same position and carry the same number and type of genes. (An exception to this rule will ...
... the same complement of genes with one member of the pair inherited from the father and the other from the mother. Chromosomes that are homologous are almost always the same size, have their centromeres in the same position and carry the same number and type of genes. (An exception to this rule will ...
Site-specific recombinase technology
Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse