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Transcriptional Repression of Hox Genes by C. elegans HP1/HPL
Transcriptional Repression of Hox Genes by C. elegans HP1/HPL

... As previously reported, HPL-2 binds to HIS-24K14me1 through its association with HPL-1, and the heterochromatin proteins HPL-1 and HPL-2 play redundant roles in C. elegans development [16–17]. Considering these observations we compared transcriptional profiles between hpl-2 (tm1489); hpl1(tm1624) do ...
Total Dissolved Solids
Total Dissolved Solids

... piece of DNA that is capable of self-replicating. In addition to one large chromosome, many bacteria naturally contain one or more plasmids. Plasmid DNA usually contains genes for one or more traits that may be beneficial to bacterial survival. In nature, bacteria can transfer plasmids back and fort ...
PDF
PDF

... multiple lines of evidence have implicated both genetic and environmental factors.3 Studies aimed at identifying the genes that contribute to interindividual blood pressure variation have been limited to candidate genes; the results have proven informative but not always consistent.4 Investigations ...
B1 6 Variation Inheritance and Cloning
B1 6 Variation Inheritance and Cloning

... These young rabbits look like their parents. This is because information about characteristics such as fur colour is passed from parents to their young. ...
DOC
DOC

... either be brown or blue. Whiles some apparently are more complex. e.g. the inheritance of the shape of the nose. Traits are heritable only if the similarity arises from shared genotypes. In experimental organisms, there’s no problem in separating environmental from genetic similarities. The offspri ...
HERITABLE AND NON-HERITABLE TRAITS Heritable traits are
HERITABLE AND NON-HERITABLE TRAITS Heritable traits are

... Heritable traits are defined by their ability to be passed from one generation to the next in a predictable manner. Visible or otherwise measurable properties of heritable traits are called phenotypes, while the genetic factors responsible for creating the phenotypes are called genotypes. The most b ...
Text S1.
Text S1.

RESEARCH COMMUNICATION Ethnicity Greatly Influences the
RESEARCH COMMUNICATION Ethnicity Greatly Influences the

... DNA sequences of the human genome reveal that many genes are polymorphic. In coding or noncoding regions of a specific gene, there may be either a single base pair substitution of one nucleotide for another (SNPs) or a variable number of repeats of a short repetitive DNA sequence (VNTR). Gene-enviro ...
Excel spreadsheet
Excel spreadsheet

... npos and nneg is binomial where p=0.1 and n=number of probe pairs for that gene. The probability of scoring npos or more of the total number of probe pairs was determined. The same analysis was done for decreased genes using nneg. The resulting probability is recorded in P(unchanged at 1 hr) t test. ...
Fundamentals of Genetics
Fundamentals of Genetics

... • should add up to 16 • What do you do to get F2 generation? • Cross two F1 individuals ...
5. Common and rare alleles 6. Genic variability of the
5. Common and rare alleles 6. Genic variability of the

... Nearly neutral polymorphisms – a common situation in many genes. Disadvantageous polymorphic alleles must be compensated for, typically by heterozygote advantage ...
Direct DNA Sequencing in the Clinical Laboratory
Direct DNA Sequencing in the Clinical Laboratory

Quantitative Genetics and Whole Genome Approaches
Quantitative Genetics and Whole Genome Approaches

... susceptible fly strain. The two strains had chromosomal markers for each chromosome. The results showed that each of the two main autosomes and the X chromosome contain QTL for DDT resistance. b. Cholesterol profiles in mice: in this example, inbred mice strains with varying cholesterol profiles wer ...
Review Transposons as tools for functional genomics
Review Transposons as tools for functional genomics

... strategies such as flanking sequence databases (described later). Secondly, complex and multiple insertions are more likely to lead to artefactual patterns of reporter gene expression when using entrapment vectors such as gene and enhancer traps. Finally, while the T-DNA approach is extremely useful ...
Supplementary Online Material
Supplementary Online Material

... Validating predicted growth-supporting genes via the experimental shRNA data As described in the main text, the set of 199 predicted growth-supporting genes are found to be ranked as highly essential based on shRNA gene knockdown data (Kolmogorov-Smirnov p-value = 0.0045) when aggregating together t ...
How Is Genetic Research On Behavior Conducted?
How Is Genetic Research On Behavior Conducted?

Document
Document

... 1. Notably we can transfer genetic information, e.g. F factor, from an F+ cell to an F- one 2. Moreover other transfers involving an Hfr cell a. These can result in incomplete transfer, forming a partial diploid bacterium (merozygote) b. Interrupting the transfer at various times allows one a way to ...
Genetics Part 1: Inheritance of Traits
Genetics Part 1: Inheritance of Traits

... up heads or tails. If you flip a coin twice, does it come up heads once and tails once? It may, but it may also come up heads twice in a row or tails twice in a row. Two heads or two tails is not what you expect. In fact, if you flip the coin four times you may even see four heads or four tails. Why ...
Microarrays and Stem Cells
Microarrays and Stem Cells

... sequence -A-G-G-T-C- to form double-stranded DNA. A computer determines the amount of sample bound to each spot on the microarray. Gene Expression Arrays An expression array allows scientists to study which genes are turned on or off at any given point in time. In this type of array, mRNA is extract ...
19. Nature vs Nurture PPT
19. Nature vs Nurture PPT

Pedigree Analysis
Pedigree Analysis

... • 2. Determine the probability of an affected offspring for a given cross. ...
Protein Synthesis Powerpoint
Protein Synthesis Powerpoint

... - Transcription: we take the code from the DNA and turn it into messenger RNA to send to the ribosome. - RNA Processing: we modify the mRNA to get rid of noncoding regions. (only in Eukaryotes!) - Translation: we use the code from the mRNA to determine which amino acids will be brought over by the t ...
Leukaemia Section t(5;12)(p13;p1 3) NIPBL/ETV6 Atlas of Genetics and Cytogenetics
Leukaemia Section t(5;12)(p13;p1 3) NIPBL/ETV6 Atlas of Genetics and Cytogenetics

... Cytogenetics molecular FISH showed that the breakpoint on 12p13 was located in ETV6. To confirm the position of the breakpoint on chromosome 5, BACs located at 5p13 were used as probes in FISH experiments. Analysis on BM cells of the patient with RP11-140A7 showed that one signal hybridized to the n ...
Slide 1 - Montville.net
Slide 1 - Montville.net

... recombinant plasmid, a plasmid with a new gene inserted. The plasmid will contain DNA from two different organisms. You will use colored paper, scissors and tape to do this. If you are successful, you will have a two colored paper ring and extra pieces of paper. ...
Gene Switches—A Lego Model
Gene Switches—A Lego Model

... Noncoding DNA: Noncoding DNA is part of DNA that is not eventually translated into amino acids. If the noncoding sections are part of a gene, they are referred to as introns. Introns are transcribed into mRNA along with exons, but are spliced out so they are never translated. DNA sequences between g ...
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Gene expression profiling



In the field of molecular biology, gene expression profiling is the measurement of the activity (the expression) of thousands of genes at once, to create a global picture of cellular function. These profiles can, for example, distinguish between cells that are actively dividing, or show how the cells react to a particular treatment. Many experiments of this sort measure an entire genome simultaneously, that is, every gene present in a particular cell.DNA microarray technology measures the relative activity of previously identified target genes. Sequence based techniques, like serial analysis of gene expression (SAGE, SuperSAGE) are also used for gene expression profiling. SuperSAGE is especially accurate and can measure any active gene, not just a predefined set. The advent of next-generation sequencing has made sequence based expression analysis an increasingly popular, ""digital"" alternative to microarrays called RNA-Seq. However, microarrays are far more common, accounting for 17,000 PubMed articles by 2006.
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