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nextgen sequencing
nextgen sequencing

... dihydroorotate dehydrogenase Validate by sequencing the gene in 3 more cases. ...
Advances and Perspectives in Genetics of Congenital Thyroid
Advances and Perspectives in Genetics of Congenital Thyroid

... transmembrane domain, and a short intracellular tail. H2O2 is used as a substrate by TPO in the organification of iodide. The H2O2 generation system of the thyroid involves a metabolic pathway which includes the complex DUOX1/DUOX2// DUOXA1/DUOXA2 [5-7]. The DUOX1 and DUOX2 genes encoding similar pr ...
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... Jeans for Genes is a charity that raises money to go to organisations that research cures into genetic diseases like cystic fibrosis. ...
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... of cradle formed by a protein associated with the ribosome: it provides a space where the polypeptide is able to undergo its folding process. • The proper folding of more complex polypeptides is aided by proteins called chaperones and ...
The molecular genetics of head development in Drosophila
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... embryonic head structures. The adult head of Drosophila is formed from three pairs of imaginal discs which develop during larval stages. Most head structures, including the head capsule, eyes, antennae and maxillary palp are derived from the eye-antennal discs (Bryant, 1978). The proboscis and the c ...
Chapter 9 - Genetics
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... • In addition to blood ‘type’, our red blood cells may (or may not) have a protein known as the Rh factor • Individuals without this factor have a “negative” blood type, while those with this factor have a “positive” blood type • Problems can occur when an Rh- mother carries an Rh+ child, especially ...
Regulatory Network Control of Blood Stem Cells
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Quantitative and Single-Gene Perspectives on the Study of Behavior
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... High-throughput data acquisition technologies in molecular biology, including rapid DNA sequencers, gene expression microarrays and other microchip-based assays, are providing an increasingly comprehensive parts list of a biological cell. Although this parts list may be far from complete at this tim ...
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Genetics Coin Toss Lab
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the extent of population exposure to assess clinical safety

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... recombinant proteins produced in living cells can undergo mutations that could alter the properties of the protein with potential adverse consequences to patients. No single experimental approach can be expected to detect all possible modifications to a protein. Protein analytical techniques can be ...
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... a gene to cells of the affected tissue. This is often done using an engineered virus as a vector. Gene therapy will only work if the normal gene is delivered to a large number of correct cells –several million in fact. Once the gene reaches its destination, it must be activated (“turned on”) to prod ...
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Gene expression profiling



In the field of molecular biology, gene expression profiling is the measurement of the activity (the expression) of thousands of genes at once, to create a global picture of cellular function. These profiles can, for example, distinguish between cells that are actively dividing, or show how the cells react to a particular treatment. Many experiments of this sort measure an entire genome simultaneously, that is, every gene present in a particular cell.DNA microarray technology measures the relative activity of previously identified target genes. Sequence based techniques, like serial analysis of gene expression (SAGE, SuperSAGE) are also used for gene expression profiling. SuperSAGE is especially accurate and can measure any active gene, not just a predefined set. The advent of next-generation sequencing has made sequence based expression analysis an increasingly popular, ""digital"" alternative to microarrays called RNA-Seq. However, microarrays are far more common, accounting for 17,000 PubMed articles by 2006.
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