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Cloning, DNA nucleotide sequence and distribution
Cloning, DNA nucleotide sequence and distribution

... gallinarum, S. moscow, S. pullorum, S. rostock, S. seremban and S. typhi, all belonging to Salmonella group D. However, expression of the SEF14 antigen was limited to S. dublin, S. enteritidis, S. moscow and S. blegdam. The nucleotide sequence of the sefA gene shared no homology with the Salmonelluf ...
Gene Section EXT2 (exostoses (multiple) 2) Atlas of Genetics and Cytogenetics
Gene Section EXT2 (exostoses (multiple) 2) Atlas of Genetics and Cytogenetics

Mendel and his Peas
Mendel and his Peas

... had to have two “factors” one from their mother and one from ...
COAT COLOR IN THE TOLLER - NSDTRC-USA
COAT COLOR IN THE TOLLER - NSDTRC-USA

... but is not necessarily so. Some mutations are "dominant" and many mutations (and more typically in purebred dogs) are "recessive." A dominant allele will change the dog's color when the dog has just one copy while a recessive allele requires two copies to change the dog's color. Some alleles are som ...
Catabolic Plasmids - UQ eSpace
Catabolic Plasmids - UQ eSpace

... degradative (catabolic) activities are encoded by large groups of genes clustered on the main chromosome or on catabolic plasmids. Such plasmids are usually large (80 to 4 200 kb) with one or more clusters of multicistronic transcriptional units, possessing up to 10–15 degradative genes. In many ins ...
Molecular markers in Brassica Rapa
Molecular markers in Brassica Rapa

... of BrCRTISO2 in a normal yellow-colored (YE) cultivar, but full-length BrCRTISO1 transcripts were not detected in the OC cultivar. Genomic sequence analysis revealed that BrCRTISO1 of the OC cultivar had many sequence variations, including single nucleotide polymorphisms (SNPs) and insertions and de ...
Single nucleotide polymorphisms and the future of genetic
Single nucleotide polymorphisms and the future of genetic

... greatly improved the ease and sophistication of such identification processes, as will be discussed in sections below. Although the frequency with which SNPs (of any kind) occur over the genome is certainly much greater than that of RFLPs alone, precise estimates are difficult to determine and often ...
lecture_07(LP)
lecture_07(LP)

... Yeast cells can normally grow on a sugar called galactose as the sole carbon source. Seven mutant “a” haploid yeast strains have been isolated that are unable to grow on galactose (“gal”) plates. Six of these mutant strains were each cross-stamped on a gal plate with a wild type “a” strain. The resu ...
Tissue- and Development-specific Expression of Multiple
Tissue- and Development-specific Expression of Multiple

... Isolation of multiple nNOS transcripts with unique 59UTRs. Cloning and sequencing of cDNA by RACE from brain, kidney, heart, intestine, and embryo led to the identification of three different nNOS mRNA species that are designated as nNOSa, nNOSb, and nNOSc. nNOSa was overall the most abundant isofor ...
Tiie Need for Bioinformatics in Evo-Devo
Tiie Need for Bioinformatics in Evo-Devo

... problem becomes that of determining levels of correlation. With enough developmental data on the underlying networks and appropriate informatics, character dependencies can be evaluated, and these can be built into the methods of analysis. This is a nontrivial undertaking that relies on heavy use of ...
7-2.5 - S2TEM Centers SC
7-2.5 - S2TEM Centers SC

... Students have had no previous instruction in genetics, but they were introduced to inherited characteristics in fourth grade (4-2.4). In sixth grade (6-3.7), students compared learned to inherited behaviors in animals. Students will study in greater detail DNA and RNA and how these substances functi ...
BIOL100 Laboratory Assignment 5: Genetics Name: Part A: Genes
BIOL100 Laboratory Assignment 5: Genetics Name: Part A: Genes

... Different  alleles  are  usually  written  “short‐hand”  using  a  system  of  lower  and  upper  case  letters.  For  instance, the different alleles of the gene for finger hair might be written as an “H” for the allele that  leads to finger hair and as an “h” for the no finger hair allele.  Capita ...
Application No. DIR 108 SUMMARY INFORMATION
Application No. DIR 108 SUMMARY INFORMATION

... (EPSPS) enzyme. EPSPS enzymes participate in a biosynthetic pathway found in both plants and microorganisms that is required for the synthesis of some essential amino acids. Most plant EPSPS enzymes are inhibited by glyphosate, which results in plant death due to the lack of essential amino acids. H ...
Survey of variation
Survey of variation

... • Eye colour is an example of ...
Practical: Ranges
Practical: Ranges

Complex Inheritance of the 5-Lipoxygenase Locus
Complex Inheritance of the 5-Lipoxygenase Locus

... the effect of chromosome 6 locus on lesion size and refine this genetic region, we created a panel of overlapping and reciprocal subcongenic lines. Overall, a total of 16 lines were constructed (Figure 1A). This was achieved by intercrossing (B6.Ldlr/ 3 CON6 Ldlr/) F1 mice and genotyping the F2 ...
Identification and Analysis of Arabidopsis Expressed Sequence
Identification and Analysis of Arabidopsis Expressed Sequence

... The fact that transcripts of this family are hormonally regulated and have unstable transcripts suggest that they may play a role in regulatory processes, although their true functions are unknown. Another interesting family of ncRNAs present in plants is typified by Mt4 in Medicago truncatula (Burl ...
Chpt20_TxnlRegChromatin.doc
Chpt20_TxnlRegChromatin.doc

... A seminal observation in the correlation of gene activity with more accessible chromatin was the demonstration that transcriptionally active genes are found in chromatin that is more sensitive to DNases. Weintraub and Groudine showed in 1976 that the overall sensitivity of a gene to DNase I is incre ...
Computational analysis of human disease
Computational analysis of human disease

... these problems, and the predictive power of computational analysis have been described previously [5–7]. Selected tools and databases that are currently available and are widely used in sequence and structure analysis are listed in Table 2. Here we focus on recent insights obtained by computational ...
Unit 5 Notes - heckgrammar.co.uk
Unit 5 Notes - heckgrammar.co.uk

...  Mendel investigated simple qualitative characteristics (or traits), such as flower colour or seed shape, and he varied one trait at a time. Previous investigators had tried to study many complex quantitative traits, such as human height or intelligence, but this is a rare instance where qualitativ ...
How to Use a Punnett Square
How to Use a Punnett Square

... List the possible genotypes and phenotypes of the offspring for this cross. The letters inside the boxes indicate probable genotypes (genetic makeup) of offspring resulting from the cross of these particular parents. There are 4 boxes, and the genotypic results should be written as percents. In this ...
Document
Document

... • Proteins have a variety of functions – Catalysis of chemical reactions (enzymes) – Structural (histones, cytoskeletal proteins) – Regulatory (transcription factors, growth factors) ...
Structural maintenance of chromosome complexes and bone
Structural maintenance of chromosome complexes and bone

... Table 1).19 Condensins mediate the compaction of interphase chromatin before mitosis. Condensin II is localized on the chromatin throughout the cell cycle and functions in the early stages of chromatin condensation in prophase. In contrast, condensin I is cytoplasmic during interphase, and gains acc ...
DOCX - Office of the Gene Technology Regulator
DOCX - Office of the Gene Technology Regulator

... The application and the proposed dealings The OGTR has received an application from the Department of Primary Industries (DPI) (Victoria) for a licence for the intentional release of a genetically modified (GM) virus resistant white clover derived from a single transformation event into the environ ...
Respiration Worksheet
Respiration Worksheet

... of autosomal dominant disorder is dwarfism, in particular, a form called Achondroplasia. Individuals with dwarfism have a defect in bone growth of the long bones, the arms and legs. As a result, the average height for Achondroplasia dwarves is about 4’ tall. Dwarfism is caused by one dominant allele ...
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Gene expression profiling



In the field of molecular biology, gene expression profiling is the measurement of the activity (the expression) of thousands of genes at once, to create a global picture of cellular function. These profiles can, for example, distinguish between cells that are actively dividing, or show how the cells react to a particular treatment. Many experiments of this sort measure an entire genome simultaneously, that is, every gene present in a particular cell.DNA microarray technology measures the relative activity of previously identified target genes. Sequence based techniques, like serial analysis of gene expression (SAGE, SuperSAGE) are also used for gene expression profiling. SuperSAGE is especially accurate and can measure any active gene, not just a predefined set. The advent of next-generation sequencing has made sequence based expression analysis an increasingly popular, ""digital"" alternative to microarrays called RNA-Seq. However, microarrays are far more common, accounting for 17,000 PubMed articles by 2006.
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