Application No. DIR 108 SUMMARY INFORMATION
... gene encodes glyphosate oxidoreductase, an enzyme capable of degrading glyphosate into nontoxic metabolites. The cp4 epsps gene encodes a 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) enzyme. EPSPS enzymes participate in a biosynthetic pathway found in both plants and microorganisms that is re ...
... gene encodes glyphosate oxidoreductase, an enzyme capable of degrading glyphosate into nontoxic metabolites. The cp4 epsps gene encodes a 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) enzyme. EPSPS enzymes participate in a biosynthetic pathway found in both plants and microorganisms that is re ...
A CRISPR-based yeast two-hybrid system for investigating
... folding, processing, modification, stabilization, and localization. Because so many cellular RNAprotein interactions remain unknown, it is advantageous to pursue their discovery using highthroughput approaches. The advent and continual improvement of high-throughput DNA sequencing technolo ...
... folding, processing, modification, stabilization, and localization. Because so many cellular RNAprotein interactions remain unknown, it is advantageous to pursue their discovery using highthroughput approaches. The advent and continual improvement of high-throughput DNA sequencing technolo ...
S4 Text.
... These are excerpts from the lab manual written by the instructor for use in our course (omitted pages/sections feature course-specific information not needed to carry out these experiments at a different institution). The two modules - 1) inverse PCR using genomic DNA extracted from adult flies for ...
... These are excerpts from the lab manual written by the instructor for use in our course (omitted pages/sections feature course-specific information not needed to carry out these experiments at a different institution). The two modules - 1) inverse PCR using genomic DNA extracted from adult flies for ...
Microcin B17 Blocks DNA Replication and Induces
... the colicins, is non-lethal for the producing cell, and is not stimulated by agents which induce the SOS response (Baquero & Moreno, 1984). In most cases microcin production is plasmiddependent and, hitherto, five types of microcins have been identified by cross-immunity, biochemical and genetic cri ...
... the colicins, is non-lethal for the producing cell, and is not stimulated by agents which induce the SOS response (Baquero & Moreno, 1984). In most cases microcin production is plasmiddependent and, hitherto, five types of microcins have been identified by cross-immunity, biochemical and genetic cri ...
Drosophila Forkhead Homologues Are Expressed in
... termed HTLF and ILF, were cloned based on binding to the HTLV-1 long terminal repeat (LTR).9,10These human FKH genes are dissimilar to the other FKH genes, and appear to bind to unrelated sequences. From the Departments of Hematology/Oncology, Biochemistry and Molecular Biology, Microbiology, and Im ...
... termed HTLF and ILF, were cloned based on binding to the HTLV-1 long terminal repeat (LTR).9,10These human FKH genes are dissimilar to the other FKH genes, and appear to bind to unrelated sequences. From the Departments of Hematology/Oncology, Biochemistry and Molecular Biology, Microbiology, and Im ...
BMI 731 Chapter1: SNP Analysis
... • The four bases pair in a set manner: Adenine (A) pairs with thymine (T), while cytosine (C) pairs with guanine (G). These pairs of bases are known as Base Pairs (bp). • The DNA is organized into separate long segments called chromosomes, where the number of chromosomes differ across organisms (46 ...
... • The four bases pair in a set manner: Adenine (A) pairs with thymine (T), while cytosine (C) pairs with guanine (G). These pairs of bases are known as Base Pairs (bp). • The DNA is organized into separate long segments called chromosomes, where the number of chromosomes differ across organisms (46 ...
Identification of a novel duplication in the APC gene using multiple
... Amsterdam, Netherlands) contains 20 paired probes from the APC region, to examine two fragments of the promoter region, 50 untranslated mRNA region and coding exons of the APC gene; the last exon is divided into three fragments (start, middle, end). Two probes for the APC wild-type sequence at mutat ...
... Amsterdam, Netherlands) contains 20 paired probes from the APC region, to examine two fragments of the promoter region, 50 untranslated mRNA region and coding exons of the APC gene; the last exon is divided into three fragments (start, middle, end). Two probes for the APC wild-type sequence at mutat ...
LESSON 4 Understanding Genetic Tests to Detect BRCA1
... similarity and regions of difference, to determine if there are any mutations in the Lawler family sequences. 22. Provide students with this analogy, if needed: Picture a factory that manufactures necklaces. Each necklace is made up of four different beads, and is about 6,000 beads long. Market rese ...
... similarity and regions of difference, to determine if there are any mutations in the Lawler family sequences. 22. Provide students with this analogy, if needed: Picture a factory that manufactures necklaces. Each necklace is made up of four different beads, and is about 6,000 beads long. Market rese ...
Quantitative genetics of gene expression during fruit fly - EMBL-EBI
... identified genetic variation associated with thousands of different traits and diseases. Yet, despite this extensive amount of research, the molecular mechanisms through which differences between genomes result in differences between whole organisms remain poorly understood. To bridge this gap betwe ...
... identified genetic variation associated with thousands of different traits and diseases. Yet, despite this extensive amount of research, the molecular mechanisms through which differences between genomes result in differences between whole organisms remain poorly understood. To bridge this gap betwe ...
Transgenic Plastids in Basic Research and Plant Biotechnology
... to effective inhibition of plastid protein biosynthesis by spectinomycin (an aminoglycoside antibiotic speci®cally blocking translation on prokaryotic-type 70 S ribosomes). A successfully transformed chloroplast expresses the spectinomycin-resistance gene, aadA, thus allowing for continued cell and ...
... to effective inhibition of plastid protein biosynthesis by spectinomycin (an aminoglycoside antibiotic speci®cally blocking translation on prokaryotic-type 70 S ribosomes). A successfully transformed chloroplast expresses the spectinomycin-resistance gene, aadA, thus allowing for continued cell and ...
The Amino Terminus of the Yeast F1-ATPase {j
... segments) that permit maintenance of the plasmids in both E. coli and S. cerevisiae. The construction of plasmid pSEY 10 I has been described (7). Plasmid pSEYC 102 is a derivative of YCp50 (gift from R. Davis, Stanford University). The unique Sma 1/Xma I site normally present in the URA3 DNA segmen ...
... segments) that permit maintenance of the plasmids in both E. coli and S. cerevisiae. The construction of plasmid pSEY 10 I has been described (7). Plasmid pSEYC 102 is a derivative of YCp50 (gift from R. Davis, Stanford University). The unique Sma 1/Xma I site normally present in the URA3 DNA segmen ...
Chp. 15
... Concept 15.1: Morgan showed that Mendelian inheritance has its physical basis in the behavior of chromosomes: Scientific inquiry The first solid evidence associating a specific gene with a specific chromosome came in the early 20th century from the work of Thomas Hunt Morgan These early experim ...
... Concept 15.1: Morgan showed that Mendelian inheritance has its physical basis in the behavior of chromosomes: Scientific inquiry The first solid evidence associating a specific gene with a specific chromosome came in the early 20th century from the work of Thomas Hunt Morgan These early experim ...
Heredity Packe
... percentage will be white? ____ What is the ratio of brown:tan:white offspring here? ___________ ...
... percentage will be white? ____ What is the ratio of brown:tan:white offspring here? ___________ ...
Candidate gene resequencing to identify rare, pedigree
... various healthy aging phenotypes. We hypothesized that pedigree-specific rare variants at longevity-associated genes could have a similar functional impact on healthy phenotypes. Methods: We performed custom hybridization capture sequencing to identify the functional variants in 464 candidate genes ...
... various healthy aging phenotypes. We hypothesized that pedigree-specific rare variants at longevity-associated genes could have a similar functional impact on healthy phenotypes. Methods: We performed custom hybridization capture sequencing to identify the functional variants in 464 candidate genes ...
BROWSING GENES AND GENOMES WITH ENSEMBL
... 8 Click on the ‘BioMart’ link on the toolbar. ... or if you are already in BioMart: 8 Click the [New] button on the toolbar. 8 Choose the ‘Ensembl Genes 69’ database. 8 Choose the ‘Homo sapiens genes (GRCh37.p8)’ dataset. 8 Click on ‘Filters’ in the left panel. 8 Expand the ‘REGION’ section by ...
... 8 Click on the ‘BioMart’ link on the toolbar. ... or if you are already in BioMart: 8 Click the [New] button on the toolbar. 8 Choose the ‘Ensembl Genes 69’ database. 8 Choose the ‘Homo sapiens genes (GRCh37.p8)’ dataset. 8 Click on ‘Filters’ in the left panel. 8 Expand the ‘REGION’ section by ...
Echinoderm conundrums: Hox genes, heterochrony, and an excess
... body plan is as follows: in all deuterostomes except echinoderms, the ectoderm and mesoderm show a high degree of A/P registry, that is, defining “anterior” from the developmental genetic perspective as being the site of the larval apical organ, a key landmark across phyla [5], the A/P identity of a ...
... body plan is as follows: in all deuterostomes except echinoderms, the ectoderm and mesoderm show a high degree of A/P registry, that is, defining “anterior” from the developmental genetic perspective as being the site of the larval apical organ, a key landmark across phyla [5], the A/P identity of a ...
A Single Gene Causes Both Male Sterility and
... identification of several genes that cause hybrid sterility or inviability—many of which have evolved rapidly under positive Darwinian selection—little is known about the ecological or genomic forces that drive the evolution of postzygotic isolation. Here, we show that the same gene, Overdrive, caus ...
... identification of several genes that cause hybrid sterility or inviability—many of which have evolved rapidly under positive Darwinian selection—little is known about the ecological or genomic forces that drive the evolution of postzygotic isolation. Here, we show that the same gene, Overdrive, caus ...
1 Depleting gene activities in early Drosophila embryos
... signaling (Peifer et al., 1991) (Figure 3). Similarly, most F1 embryos (95%) from mattub-Gal4 females crossed to UAS-shRNA-N (line HMS0009), but not from the reverse cross, showed a neurogenic phenotype (Figure 3). Note that the VALIUM22 line against N (GL00092) showed lower F1 lethality (10%), most ...
... signaling (Peifer et al., 1991) (Figure 3). Similarly, most F1 embryos (95%) from mattub-Gal4 females crossed to UAS-shRNA-N (line HMS0009), but not from the reverse cross, showed a neurogenic phenotype (Figure 3). Note that the VALIUM22 line against N (GL00092) showed lower F1 lethality (10%), most ...
homolog of the agouti gene
... acceptor sites, except for the last region which, as expected, is flanked only by a splice acceptor at its 5' end. A polypyrimidine tract and putative branch point signals 10-50 bp upstream of each conserved 3' splice acceptor site were also identified (data not shown). Most notably, the ORF defined ...
... acceptor sites, except for the last region which, as expected, is flanked only by a splice acceptor at its 5' end. A polypyrimidine tract and putative branch point signals 10-50 bp upstream of each conserved 3' splice acceptor site were also identified (data not shown). Most notably, the ORF defined ...
Dicot and monocot plants differ in retinoblastoma
... OsRBR2 gene displayed only 52% identity to OsRBR1. The full-length OsRBR2 cDNA clone was 3383 bp long and encoded a polypeptide of 978 amino acids with a calculated molecular weight of 107.9 kDa and a pI of 8.10. The OsRBR1 and OsRBR2 proteins share 52% identity in amino acid sequence, which indicat ...
... OsRBR2 gene displayed only 52% identity to OsRBR1. The full-length OsRBR2 cDNA clone was 3383 bp long and encoded a polypeptide of 978 amino acids with a calculated molecular weight of 107.9 kDa and a pI of 8.10. The OsRBR1 and OsRBR2 proteins share 52% identity in amino acid sequence, which indicat ...
Huntingtin grabs a hammer: DNA repair in HD
... Truant’s team, helmed by postdoctoral researcher Tam Maiuri, used an innovative method to pursue their hypothesis, using molecules called “chromobodies.” These can attach to specific protein targets and emit fluorescent light, illuminating working proteins that can be tracked under a microscope. In ...
... Truant’s team, helmed by postdoctoral researcher Tam Maiuri, used an innovative method to pursue their hypothesis, using molecules called “chromobodies.” These can attach to specific protein targets and emit fluorescent light, illuminating working proteins that can be tracked under a microscope. In ...
Prediction and Validation of Gene-Disease Associations
... phenotypes that share a higher than expected number of orthologous genes. In this way, a number of new, and often surprising, model systems were found for human diseases. For instance, the human neural crest related developmental disorder Waardenburg syndrome shares gene modules with gravitropism (t ...
... phenotypes that share a higher than expected number of orthologous genes. In this way, a number of new, and often surprising, model systems were found for human diseases. For instance, the human neural crest related developmental disorder Waardenburg syndrome shares gene modules with gravitropism (t ...
Expression of the Mitochondrial ATPase6 Gene and Tfam in Down
... and one or more chromosomes were displaced from the metaphase plate during the second meiotic division in 79% of oocytes in an older age group under investigation. In contrast, only 17% of the oocytes from a younger age group exhibited aneuploidy. This indicates that regulatory mechanisms responsibl ...
... and one or more chromosomes were displaced from the metaphase plate during the second meiotic division in 79% of oocytes in an older age group under investigation. In contrast, only 17% of the oocytes from a younger age group exhibited aneuploidy. This indicates that regulatory mechanisms responsibl ...
Possible consequences of the overlap between the CaMV 35S
... allergenic properties of CaMV and no similarities have been shown to know allergens, it can be concluded that the P6 protein is most likely not an allergen. In addition, a toxin database was constructed, and no significant sequence similarity with the P35S variants was detected. These data suggest t ...
... allergenic properties of CaMV and no similarities have been shown to know allergens, it can be concluded that the P6 protein is most likely not an allergen. In addition, a toxin database was constructed, and no significant sequence similarity with the P35S variants was detected. These data suggest t ...
Fungal - CBS-KNAW Fungal Biodiversity Centre
... this host (Wang et al., 1998). The unnamed Cercospora sp. reported by Crous et al. (2006) appeared to be morphologically and phylogenetically more similar to isolates in the C. apii complex than to C. zeae-maydis and C. zeina. The description of C. zeina (Crous et al., 2006) has resolved some of the ...
... this host (Wang et al., 1998). The unnamed Cercospora sp. reported by Crous et al. (2006) appeared to be morphologically and phylogenetically more similar to isolates in the C. apii complex than to C. zeae-maydis and C. zeina. The description of C. zeina (Crous et al., 2006) has resolved some of the ...
Gene
A gene is a locus (or region) of DNA that encodes a functional RNA or protein product, and is the molecular unit of heredity. The transmission of genes to an organism's offspring is the basis of the inheritance of phenotypic traits. Most biological traits are under the influence of polygenes (many different genes) as well as the gene–environment interactions. Some genetic traits are instantly visible, such as eye colour or number of limbs, and some are not, such as blood type, risk for specific diseases, or the thousands of basic biochemical processes that comprise life.Genes can acquire mutations in their sequence, leading to different variants, known as alleles, in the population. These alleles encode slightly different versions of a protein, which cause different phenotype traits. Colloquial usage of the term ""having a gene"" (e.g., ""good genes,"" ""hair colour gene"") typically refers to having a different allele of the gene. Genes evolve due to natural selection or survival of the fittest of the alleles.The concept of a gene continues to be refined as new phenomena are discovered. For example, regulatory regions of a gene can be far removed from its coding regions, and coding regions can be split into several exons. Some viruses store their genome in RNA instead of DNA and some gene products are functional non-coding RNAs. Therefore, a broad, modern working definition of a gene is any discrete locus of heritable, genomic sequence which affect an organism's traits by being expressed as a functional product or by regulation of gene expression.