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Imaging in CRISPR/Cas9 Applications
Imaging in CRISPR/Cas9 Applications

... technology by developing an inducible lentiviral system. This platform facilitates efficient gene targeting and utilizes an imagingbased tool for phenotypic assessment following deletion of essential genes and identification of tumor-promoting mutations.  The CRISPR/Cas9 strategy is based on the DN ...
Nature Rev.Mol.Cell Biol
Nature Rev.Mol.Cell Biol

... The 4.6 kb Bam HI fragment is present when the b-globin gene is inactive and histones are deacetylated ...
Chapter 3, Section 4 The DNA Connection
Chapter 3, Section 4 The DNA Connection

... • The main function of genes is to control the production of proteins in the organism’s cells. • Proteins help to determine the size, shape, and many other traits of an organism. • DNA is the major component of chromosomes. ...
X-inactivation
X-inactivation

... Active chromatin – central position in nucleus, it allows maximal efficiency of replication and transcription 2. Centromeric heterochromatin - role in centromeric function – in cohesion of sister chromatids and normal disjunction of chromatids 3. Role in epigenetic regulation of gene expression duri ...
answers
answers

... Which kind of RNA has an ANTICODON? __t-RNA____ What kind of molecules make up ribosomes? ___PROTEINS______ & ___r-RNA__________ Which cell part makes r-RNA? ___NUCLEOLUS__ Which cell part makes proteins? _RIBOSOMES______________ The ribosome makes sure the amino acid is put in the right spot by mat ...
Linkage
Linkage

... • Linkage occurs when two genes are close to each other on the same chromosome. • Linked genes are syntenic, but syntenic genes are not always linked. Genes far apart on the same chromosome assort independently: they are not linked. • Linkage is based on the frequency of crossing over between the tw ...
Intro: sequencing and the data deluge
Intro: sequencing and the data deluge

... medium aerobically vs. anaerobically • Aerobic datasets: SRR922260 • Anaerobic datasets: SRR922265 • All sequenced using Illumina GAIIx, 2x36bp PE ...
Show Me the Genes! - Brandywine School District
Show Me the Genes! - Brandywine School District

... each new cell has a complete set.  Chromosomes appear when the nucleus is no longer visible and the cell  Each cell in the body contains 46 chromosomes, so during cell division each cell will have 46 pairs of chromosomes ...
Personal genomics as a major focus of CSAIL research
Personal genomics as a major focus of CSAIL research

... - predicting likely causal variants using functional genomics  from regions to mechanism - comparative genomics annotation of coding/non-coding elements  gene regulation - relating regulatory variation to gene expression or chromatin  quantitative trait loci - measuring recent evolution and human ...
answer key for cracking the code of life
answer key for cracking the code of life

Dihybrid Crosses - Mercer Island School District
Dihybrid Crosses - Mercer Island School District

... Recombinants have new combinations of the genes. If the parental generation of cats are white furred with short tails (SS bb) and brown fur with long tails (ss BB), Parental Genotypes the F1 will be SsBb. In the gametes for the next generation, ________________ are the parental combinations for the ...
GenomePixelizer—a visualization program for comparative
GenomePixelizer—a visualization program for comparative

... added with minimal code modifications. • Images can be captured by any screenshot program and incorporated into Web pages. Images may also be saved as a PostScript file and then transformed into GIF or PNG file format. ...
Chromatin modifying activity of leukaemia associated fusion proteins
Chromatin modifying activity of leukaemia associated fusion proteins

... by basic residues (12). Phosphorylation of a site adjacent to (or nearby) a methyl mark that engages an effector module could lead to consecutive loss of binding to that factor, a mechanism that has been proposed as ‘methyl/phos switching’ (13). DNA methylation Modification of the DNA itself can lik ...
Ch. 10 DNA Review Questions
Ch. 10 DNA Review Questions

... b. RNA polymerase uses one strand of DNA as a template to assemble nucleotides into a strand of RNA. c. RNA polymerase binds only to DNA promoters, which have specific base sequences. d. Promoters are signals in RNA that indicate to RNA polymerase when to begin transcription. 5. Many RNA molecules f ...
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... form pink F1 offspring – the degree to which the dominant gene is expressed is called penetrance • Tay Sachs is a disease that causes fat accumulation in the neural tissues of children leading to death in the homozygous individual. The disease is due to the loss of function of a needed enzyme. – Alt ...
Unit 4 Mitosis, Meiosis and cell regulation
Unit 4 Mitosis, Meiosis and cell regulation

The Living Environment Unit 4 Reproduction and Development
The Living Environment Unit 4 Reproduction and Development

... Differentiation – when Mitotic cells begin to become specialized by making specific proteins (skin cells, nerve cells heart cells etc) Gene Expression – Genes begin to actively produce its special protein, thereby showing the type of cell it will be. • Gene Expression can be modified by external env ...
Gene Regulation Summary Slide Questions with
Gene Regulation Summary Slide Questions with

... 2. What is the difference between negative and positive regulation? Negative regulation is done by a repressor to impede the gene; positive regulation is done by an activator which enhances the pol/promoter interaction. A corepressor enhances the binding of a repressor, so that it repressors further ...
Genetics
Genetics

... inheritance patterns and genetic variation  Chromosome theory of Inheritance states that genes are located on chromosomes and that the behavior of chromosomes during Meiosis accounts for inheritance patterns. Mendel’s theories support this. ...
This is a paper I wrote for a documentary
This is a paper I wrote for a documentary

... switches are something that can turn parts of a gene “on” or “off”. For example, a disease that is normally associated with a certain gene, can be turned “off” by these switches on the gene. When this certain part of the gene is turned “off”, the disease does not present itself in the person. I foun ...
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... Selectable Markers. Details and examples of selectable markers ...
Diapositive 1
Diapositive 1

... description, accession number…), external links (MGI, Homologene …) and internal data (Transcriptomic data, EST, MACSIMS, MAGOS). In the future, each gene will be characterized by a retinal propensity score. The website will be designed for natural language requests through the use of our in-house d ...
DNA WebQuest - kruegerscience
DNA WebQuest - kruegerscience

... 19. What are the three regions of a gene? ______________________________ 20. What does RNA polymerase do? _________________________________ ______________________________________________________ 21. Describe the transcription process in terms of the three regions of the gene. ________ ______________ ...
Chapter 10 Workbook Notes
Chapter 10 Workbook Notes

... The operon that controls the metabolism of lactose is called the lac operon. When there is no lactose in the bacterial cell, a repressor turns the operon off. A repressor is a protein that binds to an operator and physically blocks RNA polymerase from binding to a promoter site. Repression of Transc ...
Poster Title - Northern New Mexico College
Poster Title - Northern New Mexico College

... method that not only computes the pvalue of a gene set but also the conditional probability or dependence of genes, P(A|B). P(A|B) is the probability that gene A is differentially expressed given that gene B is differentially expressed. These dependencies will allow us to construct gene sets. Our pr ...
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Epigenetics of human development

Development before birth, including gametogenesis, embryogenesis, and fetal development, is the process of body development from the gametes are formed to eventually combine into a zygote to when the fully developed organism exits the uterus. Epigenetic processes are vital to fetal development due to the need to differentiate from a single cell to a variety of cell types that are arranged in such a way to produce cohesive tissues, organs, and systems.Epigenetic modifications such as methylation of CpGs (a dinucleotide composed of a 2'-deoxycytosine and a 2' deoxyguanosine) and histone tail modifications allow activation or repression of certain genes within a cell, in order to create cell memory either in favor of using a gene or not using a gene. These modifications can either originate from the parental DNA, or can be added to the gene by various proteins and can contribute to differentiation. Processes that alter the epigenetic profile of a gene include production of activating or repressing protein complexes, usage of non-coding RNAs to guide proteins capable of modification, and the proliferation of a signal by having protein complexes attract either another protein complex or more DNA in order to modify other locations in the gene.
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