Fulltext PDF - Indian Academy of Sciences
... reaction (RT-PCR) was performed by the RT-PCR kit (Promega, USA). The first strand cDNA was synthesized from 3 ng total RNA according to the manufacturer’s recommendations. Internal cDNA was amplified with ERF-F (5′-ATCCAGGGAAAAAGAGCCGTG-3′) and ERF-R (5′CAACGCGAGCGAATGATTTCT-3′), which were designe ...
... reaction (RT-PCR) was performed by the RT-PCR kit (Promega, USA). The first strand cDNA was synthesized from 3 ng total RNA according to the manufacturer’s recommendations. Internal cDNA was amplified with ERF-F (5′-ATCCAGGGAAAAAGAGCCGTG-3′) and ERF-R (5′CAACGCGAGCGAATGATTTCT-3′), which were designe ...
population
... 1. What are the three mechanisms for altering allele frequency? 2. What is the type of selection reflecting the fact that really small wolves and really big ...
... 1. What are the three mechanisms for altering allele frequency? 2. What is the type of selection reflecting the fact that really small wolves and really big ...
Document
... • Fourth (now known as the law of segregation): the two alleles for a heritable character separate (segregate) during gamete formation and end up in different gametes • Thus, an egg or a sperm gets only one of the two alleles that are present in the organism • This segregation of alleles correspond ...
... • Fourth (now known as the law of segregation): the two alleles for a heritable character separate (segregate) during gamete formation and end up in different gametes • Thus, an egg or a sperm gets only one of the two alleles that are present in the organism • This segregation of alleles correspond ...
Pedigree
... • A baby born with Tay-Sachs usually seems healthy for the first few months, but as nerve cells become packed down with fatty material a relentless deterioration of mental and physical abilities begins to occur. ...
... • A baby born with Tay-Sachs usually seems healthy for the first few months, but as nerve cells become packed down with fatty material a relentless deterioration of mental and physical abilities begins to occur. ...
Investigation of Four Genes Responsible for Autosomal Recessive
... genes already described in ARCC and highly expressed in the human brain particularly during embryogenesis (EPHA2, GALK1, GCNT2, and CRYBB1). EPHA2 belongs to the tyrosine kinase family, and the protein EphA2 is an epithelial cell kinase that interacts with membrane-bound ephrin ligands, which play a ...
... genes already described in ARCC and highly expressed in the human brain particularly during embryogenesis (EPHA2, GALK1, GCNT2, and CRYBB1). EPHA2 belongs to the tyrosine kinase family, and the protein EphA2 is an epithelial cell kinase that interacts with membrane-bound ephrin ligands, which play a ...
Conditional (if else) lecture
... • A Hash table can be often used like an reference index ; e.g. “code of life” translation table : – hash_base.pl shows what the nucleotide base letter stands for. – Moreover Hash tables could be use, as it the exercise, to create a DNA codon conversion table so that when a codon is encountered as i ...
... • A Hash table can be often used like an reference index ; e.g. “code of life” translation table : – hash_base.pl shows what the nucleotide base letter stands for. – Moreover Hash tables could be use, as it the exercise, to create a DNA codon conversion table so that when a codon is encountered as i ...
X r Y
... – The factors separated when the gametes were formed during meiosis, each gamete would get either the tall or short gene. – When random fusion of the gametes occurred during fertilization, the combinations were brought together in a 3:1 ratio, as indicated by the Punnett square. ...
... – The factors separated when the gametes were formed during meiosis, each gamete would get either the tall or short gene. – When random fusion of the gametes occurred during fertilization, the combinations were brought together in a 3:1 ratio, as indicated by the Punnett square. ...
Klug9chapt03
... 3.4 The Trihybrid Cross Demonstrates That Mendel’s Principles Apply to Inheritance of Multiple Traits 3.4.1 The Forked-Line Method, or Branch Diagram ...
... 3.4 The Trihybrid Cross Demonstrates That Mendel’s Principles Apply to Inheritance of Multiple Traits 3.4.1 The Forked-Line Method, or Branch Diagram ...
Nongenic transcription, gene regulation and action at a distance
... the retrotransposons in those repeats also contain functional promoters. However, most are probably just ‘junk’ and destroyed rapidly by the nonsense-mediated decay pathway (Iborra et al., 2001). I will argue that nature utilizes neighbouring transcription units (whether they be genic or nongenic) i ...
... the retrotransposons in those repeats also contain functional promoters. However, most are probably just ‘junk’ and destroyed rapidly by the nonsense-mediated decay pathway (Iborra et al., 2001). I will argue that nature utilizes neighbouring transcription units (whether they be genic or nongenic) i ...
Characterization of PIR1, a GATA family transcription factor involved
... for potential gDNA contamination, RT-minus reactions were carried out in real time using SYBR Green detection chemistry. No quantification cycle (Cq) values were obtained, at least under the cycle conditions used (see below). Thereafter, mRNA quantification was determined using RiboGreen dye (Invitrog ...
... for potential gDNA contamination, RT-minus reactions were carried out in real time using SYBR Green detection chemistry. No quantification cycle (Cq) values were obtained, at least under the cycle conditions used (see below). Thereafter, mRNA quantification was determined using RiboGreen dye (Invitrog ...
Lack of expression of XIST from a small ring X chromosome
... and PM2, PCR products were digested with 15 U of BanI, and resolved on polyacrylamide gels. In the presence of the 743A, 16 bp were removed from the end of the 145 bp ...
... and PM2, PCR products were digested with 15 U of BanI, and resolved on polyacrylamide gels. In the presence of the 743A, 16 bp were removed from the end of the 145 bp ...
A Novel Mouse Chromosome 17 Hybrid Sterility Locus
... rise to equilibrium allele frequencies of5-1 5% in most populations that have been studied (FICUEROA et al. 1988a). Since their discovery over 60 years ago, the origin and evolution of these unusual genetic entities has intrigued many investigators. Until the last decade, the question of t haplotype ...
... rise to equilibrium allele frequencies of5-1 5% in most populations that have been studied (FICUEROA et al. 1988a). Since their discovery over 60 years ago, the origin and evolution of these unusual genetic entities has intrigued many investigators. Until the last decade, the question of t haplotype ...
Slide 1
... inheritance of a single character Mendel developed four hypotheses (using modern terminology) 1. Alleles are alternative versions of genes that account for variations in inherited characters. 2. For each characteristic, an organism inherits two alleles (on homologs), one from each parent. The alle ...
... inheritance of a single character Mendel developed four hypotheses (using modern terminology) 1. Alleles are alternative versions of genes that account for variations in inherited characters. 2. For each characteristic, an organism inherits two alleles (on homologs), one from each parent. The alle ...
F 1 generation
... inheritance of a single character Mendel developed four hypotheses (using modern terminology) 1. Alleles are alternative versions of genes that account for variations in inherited characters. 2. For each characteristic, an organism inherits two alleles (on homologs), one from each parent. The alle ...
... inheritance of a single character Mendel developed four hypotheses (using modern terminology) 1. Alleles are alternative versions of genes that account for variations in inherited characters. 2. For each characteristic, an organism inherits two alleles (on homologs), one from each parent. The alle ...
Document
... xv. They tend to have megacolon. They can’t push things through that well so they don’t have good peristalsis. xvi. They are also at risk for having atresia or closing off of their duodenum. xvii. [S14] The most important thing for us to remember is that 40% of Down syndrome babies have congenital h ...
... xv. They tend to have megacolon. They can’t push things through that well so they don’t have good peristalsis. xvi. They are also at risk for having atresia or closing off of their duodenum. xvii. [S14] The most important thing for us to remember is that 40% of Down syndrome babies have congenital h ...
Using Disruptive Selection to Maintain Diversity in Genetic Algorithms
... the main schemes used in GAs. The resulting population, sometimes called the intermediate population, is then processed using crossover and mutation to form the next generation. 2. Crossover: A crossover operator manipulates a pair of individuals (called parents) to produce two new individuals (call ...
... the main schemes used in GAs. The resulting population, sometimes called the intermediate population, is then processed using crossover and mutation to form the next generation. 2. Crossover: A crossover operator manipulates a pair of individuals (called parents) to produce two new individuals (call ...
Lung Cancer Research Update
... Over the past four years, more than 1,000 lung cancer patients and their relatives have participated in our study. The efforts of these participants and our research team have led to three national grant awards for research and more than 10 scientific publications. These achievements would not have ...
... Over the past four years, more than 1,000 lung cancer patients and their relatives have participated in our study. The efforts of these participants and our research team have led to three national grant awards for research and more than 10 scientific publications. These achievements would not have ...
TEV_v7_BY
... (Figure 3b). Assuming that TEVs inserted in the antisense orientation are not under selection, then approximately 50% of all ERV insertions in the sense orientation into the introns of protein coding genes are deleterious, as are about one-third of LINE or SINE sense insertions. The large set of TEV ...
... (Figure 3b). Assuming that TEVs inserted in the antisense orientation are not under selection, then approximately 50% of all ERV insertions in the sense orientation into the introns of protein coding genes are deleterious, as are about one-third of LINE or SINE sense insertions. The large set of TEV ...
Eds., N. Hamamura, S. Suzuki, S. Mendo, C. M. Barroso,... © by TERRAPUB, 2010.
... Abstract—Mercury resistance is the only bacterial metal resistance system whose mechanism leads to a delicate transformation of its toxic target. The mechanism involves the reduction of the highly reactive cationic form of mercury into metallic vapor. The genes, which are responsible for this resist ...
... Abstract—Mercury resistance is the only bacterial metal resistance system whose mechanism leads to a delicate transformation of its toxic target. The mechanism involves the reduction of the highly reactive cationic form of mercury into metallic vapor. The genes, which are responsible for this resist ...
126 Kb
... These answers make good sense, but they betray a mammal-centric view of life: none begins to explain why something as large as a mammal should have evolved in the first place. The question I’m interested in answering is not whether large mammals are better adapted than small mammals, but why it was ...
... These answers make good sense, but they betray a mammal-centric view of life: none begins to explain why something as large as a mammal should have evolved in the first place. The question I’m interested in answering is not whether large mammals are better adapted than small mammals, but why it was ...
THE LOD SCORE METHOD
... The value between 0 < < 0.5 resulting in the highest LOD score is called the maximum likelihood estimate (MLE) ( ) of the recombination fraction. If one or more LOD scores are reasonably large, the largest would give a clue as to the correct recombination frequency. Linkage is considered likely i ...
... The value between 0 < < 0.5 resulting in the highest LOD score is called the maximum likelihood estimate (MLE) ( ) of the recombination fraction. If one or more LOD scores are reasonably large, the largest would give a clue as to the correct recombination frequency. Linkage is considered likely i ...
Differential activity of Rickettsia rickettsii ompA and ompB promoter
... homology within their respective C-terminal 320 amino acids ( > 36% identity), a region that may include a membrane anchor (Hackstadt e t al., 1992) in the last 10 amino acids of each protein. It appears that in the case of rOmpB, a precursor form of the protein is cleaved to yield a 135 kDa N-termi ...
... homology within their respective C-terminal 320 amino acids ( > 36% identity), a region that may include a membrane anchor (Hackstadt e t al., 1992) in the last 10 amino acids of each protein. It appears that in the case of rOmpB, a precursor form of the protein is cleaved to yield a 135 kDa N-termi ...