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Saturation
Saturation

... Doppler Width Consider an excited molecule with a velocity v relative to the rest fame of the observer. The central frequency of the emission is w At the rest frame the frequency is ...
SPDM: light microscopy with single
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[pdf]

Viewing Microstructures of Materials using the Optical Microscope
Viewing Microstructures of Materials using the Optical Microscope

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Optical imaging using binary sensors Aur´elien Bourquard, Franc¸ois Aguet, and Michael Unser

On the conservation of fundamental optical quantities in non
On the conservation of fundamental optical quantities in non

... Next, we can do the same for the angular momentum, as shown in figure 3, where again the four situations, now distinctively different, have been plotted. Again, the total angular momentum in the z direction, denoted by Lz, depends on the numerical aperture of the imaging system when spin angular momen ...
Microscopy Overview
Microscopy Overview

... source is usually used. In critical illumination, the source is focused on to the object by a condenser lens. The disadvantage of this approach is that variations in emission of the source are imaged directly into the image. Cheaper microscopes avoid this problem by using a diffuser. Better microsco ...
Scanning Electron Microscope - i-Explore International Research
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Fluorescence Spectroscopy 1.0 Emission – the mirror image
Fluorescence Spectroscopy 1.0 Emission – the mirror image

... 3. Electron transfer – 1DA  D+A4. Energy transfer – emission is transferred to an acceptor Fluorescence quenching can be both beneficial and a source of error in experiments. Since fluorescence is subject to quenching, one must be careful to account for any possible fluorescence quenching that may ...
A tunable low-energy photon source for high
A tunable low-energy photon source for high

... the external lens must be within 95 mm of the monochromator exit slit. Choosing focal lengths of 65 mm (external lens) and 130 mm (internal lens), we form an image of the exit slit at the sample position with a 2× magnification. In the diagram of Fig. 1, the focusing lens is shown within the UHV cha ...
Electron Induced Fluorescence Spectra of Methane
Electron Induced Fluorescence Spectra of Methane

Solid-State and High-Resolution Liquid 119Sn NMR Spectroscopy
Solid-State and High-Resolution Liquid 119Sn NMR Spectroscopy

Plasmonic modes of gold nano-particle arrays on thin gold
Plasmonic modes of gold nano-particle arrays on thin gold

... arrays with varying array periods. The reference for calculating the extinction is taken on the gold film outside the arrays. The thin line depicts the extinction spectrum of a sample with random particle distribution (see text); (d) Resonance wavelengths of the extinction peaks vs. array period: ex ...
肖连团 - 山西大学
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... Introduction • In view of quantum optical operations with photons and emitters, it would be highly desirable for as many of the incident photons as possible to interact with a single emitter. • Such a regime would open the door to a wealth of nonlinear interactions between single emitters and singl ...
chapter ia brief overview of structural, spectral and
chapter ia brief overview of structural, spectral and

... to be an interesting field of research. Investigations have been done on these compounds in the field of crystallographic studies whereas, much of complete vibrational analysis and molecular properties using DFT and biological activities on these are yet to be known. With this idea in mind, six numb ...
A guide to super-resolution fluorescence microscopy
A guide to super-resolution fluorescence microscopy

ULTRA-HIGH DENSITY OPTICAL DATA STORAGE P.R. Hemmer
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Novel high-temperature and pressure-compatible ultrasonic levitator
Novel high-temperature and pressure-compatible ultrasonic levitator

... to observe the Raman-active symmetric stretching modes of aromatic rings are further complementary aspects to infrared absorption spectroscopy. The combination of the complimentary Raman and FTIR spectroscopic probes, the pressure-compatible process chamber, heating by a carbon dioxide laser, and th ...
كيمياء الصناعية (1)
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... of atomic orbitals) theory. Statistical thermodyamics. Some aspects of molecular structure and bonding; chemistry of hydrogen; chemistry of the main group elements: IA (alkali); IIA (alkaline earth); IIIAVIA-VIA; VIIA (halogens); VIII (noble gases); Lanthanaides and Actanides Multistep syntheses; cl ...
chemical potential dependence of particle ratios within a
chemical potential dependence of particle ratios within a

... the experimental data. It is seen that the ratios increase towards unity with an increase in collision energies and with a decrease in the chemical potential. The ratios appear ordered with the strangeness quantum number, i. e., the higher the strangeness quantum number is, the smaller the difference ...
Interferometric back focal plane microellipsometry
Interferometric back focal plane microellipsometry

... in Fig. 5. In the Au and Al data, d increases and decreases along the horizontal and vertical directions, respectively, as expected from Eqs. ~17!, and the nodal lines along the diagonals as predicted in Eq. ~18! are clearly visible. The averages of the four d lines given in Eqs. ~17! from the diffe ...
Photoelectron imaging of modal interference in
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Download PDF
Download PDF

Diffuse optical imaging
Diffuse optical imaging

... absorption spectra of oxy- and deoxyhaemoglobin (HbO and HHb, respectively) to assess blood oxygenation. This demonstrated that biomedical optics could provide clinically useful information by examination of the absorption of tissue, provided that the confounding effects of scatter were minimized, b ...
Investigation of nanoscale structural alterations of cell nucleus as an
Investigation of nanoscale structural alterations of cell nucleus as an

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Chemical imaging

Chemical imaging (as quantitative – chemical mapping) is the analytical capability to create a visual image of components distribution from simultaneous measurement of spectra and spatial, time information.The main idea - for chemical imaging, the analyst may choose to take as many data spectrum measured at a particular chemical component in spatial location at time; this is useful for chemical identification and quantification. Alternatively, selecting an image plane at a particular data spectrum (PCA - multivariable data of wavelength, spatial location at time) can map the spatial distribution of sample components, provided that their spectral signatures are different at the selected data spectrum.Software for chemical imaging is most specific and distinguished from chemical methods such as chemometrics. Hyperspectral imaging is most often applied to either solid or gel samples, and has applications in chemistry, biology, medicine, pharmacy (see also for example: food science, biotechnology, agriculture and industry. NIR, IR and Raman chemical imaging is also referred to as hyperspectral, spectroscopic, spectral or multispectral imaging (also see microspectroscopy). However, other ultra-sensitive and selective imaging techniques are also in use that involve either UV-visible or fluorescence microspectroscopy. Many imaging techniques can be used to analyze samples of all sizes, from the single molecule to the cellular level in biology and medicine, and to images of planetary systems in astronomy, but different instrumentation is employed for making observations on such widely different systems.Imaging instrumentation has three components: a radiation source to illuminate the sample, a spectrally selective element, and usually a detector array (the camera) to collect the images. When many stacked spectral channels (wavelengths) are collected for different locations of the microspectrometer focus on a line or planar array in the focal plane, the data is called hyperspectral; fewer wavelength data sets are called multispectral. The data format is called a hypercube. The data set may be visualized as a data cube, a three-dimensional block of data spanning two spatial dimensions (x and y), with a series of wavelengths (lambda) making up the third (spectral) axis. The hypercube can be visually and mathematically treated as a series of spectrally resolved images (each image plane corresponding to the image at one wavelength) or a series of spatially resolved spectra. Many materials, both manufactured and naturally occurring, derive their functionality from the spatial distribution of sample components. For example, extended release pharmaceutical formulations can be achieved by using a coating that acts as a barrier layer. The release of active ingredient is controlled by the presence of this barrier, and imperfections in the coating, such as discontinuities, may result in altered performance. In the semi-conductor industry, irregularities or contaminants in silicon wafers or printed micro-circuits can lead to failure of these components. The functionality of biological systems is also dependent upon chemical gradients – a single cell, tissue, and even whole organs function because of the very specific arrangement of components. It has been shown that even small changes in chemical composition and distribution may be an early indicator of disease. Any material that depends on chemical gradients for functionality may be amenable to study by an analytical technique that couples spatial and chemical characterization. To efficiently and effectively design and manufacture such materials, the ‘what’ and the ‘where’ must both be measured. The demand for this type of analysis is increasing as manufactured materials become more complex. Chemical imaging techniques is critical to understanding modern manufactured products and in some cases is a non-destructive technique so that samples are preserved for further testing.
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