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Cambrian Explosion of Life: the Big Bang in Metazoan Evolution
Cambrian Explosion of Life: the Big Bang in Metazoan Evolution

... substitutions in genes arising out of mutation are selectively neutral or of little functional consequence to the organisms. This theory was slightly modified later to accommodate the observation that most molecular evolution involves slightly deleterious substitutions rather than strictly neutral o ...
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Monomeric Cyanine Nucleic Acid Stains
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AP Biology
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History of molecular evolution

The history of molecular evolution starts in the early 20th century with ""comparative biochemistry"", but the field of molecular evolution came into its own in the 1960s and 1970s, following the rise of molecular biology. The advent of protein sequencing allowed molecular biologists to create phylogenies based on sequence comparison, and to use the differences between homologous sequences as a molecular clock to estimate the time since the last common ancestor. In the late 1960s, the neutral theory of molecular evolution provided a theoretical basis for the molecular clock, though both the clock and the neutral theory were controversial, since most evolutionary biologists held strongly to panselectionism, with natural selection as the only important cause of evolutionary change. After the 1970s, nucleic acid sequencing allowed molecular evolution to reach beyond proteins to highly conserved ribosomal RNA sequences, the foundation of a reconceptualization of the early history of life.
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