lecture_23 - supporting lehigh cse
... All HDPP’s paths are equally likely to be formed during the random production of sequences In other words, over a large well distributed solution set, all solutions (or at least a great majority) should be present *This is key because in order for the DNA computer to arrive at the correct solution, ...
... All HDPP’s paths are equally likely to be formed during the random production of sequences In other words, over a large well distributed solution set, all solutions (or at least a great majority) should be present *This is key because in order for the DNA computer to arrive at the correct solution, ...
Chapter 1
... explains why the molecules of living organism are mainly made of only a few elements. Proteins are made up of chains of amino acids, some of which are spontaneously formed in a pre-biotic world. The sugar molecules in a cell are also frequently interconnected into chains of carbohydrates, the second ...
... explains why the molecules of living organism are mainly made of only a few elements. Proteins are made up of chains of amino acids, some of which are spontaneously formed in a pre-biotic world. The sugar molecules in a cell are also frequently interconnected into chains of carbohydrates, the second ...
DNA Scissors: Introduction to Restriction
... 2. Now separate the hydrogen bonds between the cut sites by cutting through the vertical lines. Separate the two pieces of DNA. Look at the new DNA ends produced by EcoRI. Are they sticky or blunt? Write EcoRI on the cut ends. Keep the cut fragments on your desk. 3. Repeat the procedure with strip 2 ...
... 2. Now separate the hydrogen bonds between the cut sites by cutting through the vertical lines. Separate the two pieces of DNA. Look at the new DNA ends produced by EcoRI. Are they sticky or blunt? Write EcoRI on the cut ends. Keep the cut fragments on your desk. 3. Repeat the procedure with strip 2 ...
Chromosomal Theory and Genetic Linkage
... segment. Using mutant ies with structurally distinct X chromosomes was the key to observing the products of recombination because DNA sequencing and other molecular tools were not yet available. It is now known that homologous chromosomes regularly exchange segments in meiosis by reciprocally break ...
... segment. Using mutant ies with structurally distinct X chromosomes was the key to observing the products of recombination because DNA sequencing and other molecular tools were not yet available. It is now known that homologous chromosomes regularly exchange segments in meiosis by reciprocally break ...
Chromosomal Theory and Genetic Linkage
... segment. Using mutant ies with structurally distinct X chromosomes was the key to observing the products of recombination because DNA sequencing and other molecular tools were not yet available. It is now known that homologous chromosomes regularly exchange segments in meiosis by reciprocally break ...
... segment. Using mutant ies with structurally distinct X chromosomes was the key to observing the products of recombination because DNA sequencing and other molecular tools were not yet available. It is now known that homologous chromosomes regularly exchange segments in meiosis by reciprocally break ...
An homologous pair of chromosomes…
... Neighbouring non-sister chromatids are cut at the same point. A Holliday junction forms as the DNA of the cut sections attach to the open end of the opposite non-sister chromatid. ...
... Neighbouring non-sister chromatids are cut at the same point. A Holliday junction forms as the DNA of the cut sections attach to the open end of the opposite non-sister chromatid. ...
A comprehensive computational model of facilitated diffusion in
... In addition, previous simulators did not take into account TF orientation on the DNA (Barnes and Chu, 2010; Chu et al., 2009). The orientation of TFs affects the affinity of the TF for a specific position on the DNA, i.e. a molecule bound in one orientation can have a totally different affinity comp ...
... In addition, previous simulators did not take into account TF orientation on the DNA (Barnes and Chu, 2010; Chu et al., 2009). The orientation of TFs affects the affinity of the TF for a specific position on the DNA, i.e. a molecule bound in one orientation can have a totally different affinity comp ...
Bioreg2017_Replication1_V3
... For each substrate, the molecular flux (and hence molecular choice) is determined by the ratio of the forward to discard rate constants (black arrows) for that substrate. For cognate substrates this ratio should favor the forward reaction. For noncognate substrates, the ratio should “flip” to favor ...
... For each substrate, the molecular flux (and hence molecular choice) is determined by the ratio of the forward to discard rate constants (black arrows) for that substrate. For cognate substrates this ratio should favor the forward reaction. For noncognate substrates, the ratio should “flip” to favor ...
lab9
... – Use of water will produce no migraton – High buffer conc. could melt the agarose gel – New Sodium Borate (SB) buffer allows gels to be run at higher voltages in less time than traditional buffers • Agarose gel, a porous material that DNA migrates through • Gel casting materials • DNA ladder, mixtu ...
... – Use of water will produce no migraton – High buffer conc. could melt the agarose gel – New Sodium Borate (SB) buffer allows gels to be run at higher voltages in less time than traditional buffers • Agarose gel, a porous material that DNA migrates through • Gel casting materials • DNA ladder, mixtu ...
Structural analysis of both products of a reciprocal translocation
... that occurs In a human cell (1-3). Such analyses are Important because they r e f l e c t the mechanisms by which these translocations occur and allow us to explore the structural and functional consequences of juxtaposing genes normally located on different chromosomes. Several reciprocal transloca ...
... that occurs In a human cell (1-3). Such analyses are Important because they r e f l e c t the mechanisms by which these translocations occur and allow us to explore the structural and functional consequences of juxtaposing genes normally located on different chromosomes. Several reciprocal transloca ...
Where Is DNA Found?
... People in the News Sir Alec Jeffreys is credited with developing DNA profiling using RFLP. In September of 1984, after years of work, he saw his first series of blots on an X ray. The technique was first used in forensics when, in 1985, he was asked by police to confirm the rape confession of 17-yea ...
... People in the News Sir Alec Jeffreys is credited with developing DNA profiling using RFLP. In September of 1984, after years of work, he saw his first series of blots on an X ray. The technique was first used in forensics when, in 1985, he was asked by police to confirm the rape confession of 17-yea ...
DNA Analysis
... People in the News Sir Alec Jereys is credited with developing DNA profiling using RFLP. In September of 1984, after years of work, he saw his first series of blots on an X ray. The technique was first used in forensics when, in 1985, he was asked by police to confirm the rape confession of 17-year ...
... People in the News Sir Alec Jereys is credited with developing DNA profiling using RFLP. In September of 1984, after years of work, he saw his first series of blots on an X ray. The technique was first used in forensics when, in 1985, he was asked by police to confirm the rape confession of 17-year ...
Forensics Ch 12
... People in the News Sir Alec Jeffreys is credited with developing DNA profiling using RFLP. In September of 1984, after years of work, he saw his first series of blots on an X ray. The technique was first used in forensics when, in 1985, he was asked by police to confirm the rape confession of 17-yea ...
... People in the News Sir Alec Jeffreys is credited with developing DNA profiling using RFLP. In September of 1984, after years of work, he saw his first series of blots on an X ray. The technique was first used in forensics when, in 1985, he was asked by police to confirm the rape confession of 17-yea ...
Western Blot - Faperta UGM
... A simple rapid, sensitive and versatile in vitro method for selectively amplifying defined sequences/regions of DNA/RNA from an initial complex source of nucleic acid - generates sufficient for subsequent analysis and/or manipulation Amplification of a small amount of DNA using specific DNA prim ...
... A simple rapid, sensitive and versatile in vitro method for selectively amplifying defined sequences/regions of DNA/RNA from an initial complex source of nucleic acid - generates sufficient for subsequent analysis and/or manipulation Amplification of a small amount of DNA using specific DNA prim ...
unit II - SP College
... Satellite DNA adopts higher-order three-dimensional structures in eukaryotic organisms. This was demonstrated in the land crab Gecarcinuslateralis, whose DNA contains 3% of a GC-rich sequence consisting of repeats of a ~2100 base pair (bp) sequence called RU. The RU was arranged in long tandem array ...
... Satellite DNA adopts higher-order three-dimensional structures in eukaryotic organisms. This was demonstrated in the land crab Gecarcinuslateralis, whose DNA contains 3% of a GC-rich sequence consisting of repeats of a ~2100 base pair (bp) sequence called RU. The RU was arranged in long tandem array ...
Creating 3-Dimensional Graph Structures with DNA
... Seeman [9] suggested two physical models for exploring 3D DNA structures and the plausibility of their construction. We built the structure we propose (type I edge building block) using one of his models, as shown in Fig. 13. This supports the plausibility of our construction. It could be simpler to ...
... Seeman [9] suggested two physical models for exploring 3D DNA structures and the plausibility of their construction. We built the structure we propose (type I edge building block) using one of his models, as shown in Fig. 13. This supports the plausibility of our construction. It could be simpler to ...
Where Is DNA Found?
... jointly received the Nobel Prize in 1962 for their determination of the structure of DNA. What is interesting about this fact is that Rosalind Franklin had as much to do with the discovery as the other three gentlemen with her work with X-ray crystallography. She died of cancer and could not be hono ...
... jointly received the Nobel Prize in 1962 for their determination of the structure of DNA. What is interesting about this fact is that Rosalind Franklin had as much to do with the discovery as the other three gentlemen with her work with X-ray crystallography. She died of cancer and could not be hono ...
File
... DNA at Work • DNA directs the production of proteins, which are made by combining amino acids. • The sequence of amino acids in a protein chain determines the shape and function of the protein. • Each group of three nucleotides in a DNA sequence codes for a particular amino acid. – Example: G-A-G c ...
... DNA at Work • DNA directs the production of proteins, which are made by combining amino acids. • The sequence of amino acids in a protein chain determines the shape and function of the protein. • Each group of three nucleotides in a DNA sequence codes for a particular amino acid. – Example: G-A-G c ...
DNA -‐ Compsci 201
... The code below finds all occurrences of a restriction enzyme like “gaattc” and splices in a new strand of DNA, represented by parameter splicee to create a recombinant strand. The strand ...
... The code below finds all occurrences of a restriction enzyme like “gaattc” and splices in a new strand of DNA, represented by parameter splicee to create a recombinant strand. The strand ...
Nucleic Acids Lecture Notes_rev1
... alternating co-polymers can form Z-DNA (G-C)2, the compositional isomer, dG.dC, does not form Z-DNA at all. The formation of Z-DNA is solvent dependent. In oligonucleotides, it forms only in the presence of alcohol and either very high or very low salt. Moreover, as we shall see, the salt concentrat ...
... alternating co-polymers can form Z-DNA (G-C)2, the compositional isomer, dG.dC, does not form Z-DNA at all. The formation of Z-DNA is solvent dependent. In oligonucleotides, it forms only in the presence of alcohol and either very high or very low salt. Moreover, as we shall see, the salt concentrat ...
Genetic recombination
... Inter-chromosomal – between nonhomologous chromosomes; Intra-chromosomal – between homologous chromosomes ...
... Inter-chromosomal – between nonhomologous chromosomes; Intra-chromosomal – between homologous chromosomes ...
The Structure of the Human AGT Protein Bound to DNA
... Protein/DNA interactions and implications for the base-flipping mechanism Globally, hAGT makes three major contacts with DNA, one to each phosphate backbone and a third along the groove (Figures 4 and 5(c)). In the first interaction a phosphate group on the strand containing the flipped base is brac ...
... Protein/DNA interactions and implications for the base-flipping mechanism Globally, hAGT makes three major contacts with DNA, one to each phosphate backbone and a third along the groove (Figures 4 and 5(c)). In the first interaction a phosphate group on the strand containing the flipped base is brac ...
MHF1 plays Fanconi anaemia complementation group M protein
... mhf1-1 and mhf1-2 single mutants did not exhibit an elevated sensitivity to the cross-linking agents cisplatin and MMC (Figure 2a,b). Furthermore, neither mutant was hypersensitive to bleomycin, hydroxyurea or MMS, respectively (Figure S2). This result, although different from the phenotype of the a ...
... mhf1-1 and mhf1-2 single mutants did not exhibit an elevated sensitivity to the cross-linking agents cisplatin and MMC (Figure 2a,b). Furthermore, neither mutant was hypersensitive to bleomycin, hydroxyurea or MMS, respectively (Figure S2). This result, although different from the phenotype of the a ...
Point mutation of bacterial artificial chromosomes by ET recombination
... contained a homology arm (a or b), followed by the region that primed the amplification of sBn. In addition, the 3′ oligonucleotide contained a C nucleotide, coding for the G to be introduced, between the homology arm b and the PCR priming region. Since the first 5 nucleotides of homology arm b were ...
... contained a homology arm (a or b), followed by the region that primed the amplification of sBn. In addition, the 3′ oligonucleotide contained a C nucleotide, coding for the G to be introduced, between the homology arm b and the PCR priming region. Since the first 5 nucleotides of homology arm b were ...
Holliday junction
A Holliday junction is a branched nucleic acid structure that contains four double-stranded arms joined together. These arms may adopt one of several conformations depending on buffer salt concentrations and the sequence of nucleobases closest to the junction. The structure is named after the molecular biologist Robin Holliday, who proposed its existence in 1964.In biology, Holliday junctions are a key intermediate in many types of genetic recombination, as well as in double-strand break repair. These junctions usually have a symmetrical sequence and are thus mobile, meaning that the four individual arms may slide though the junction in a specific pattern that largely preserves base pairing. Additionally, four-arm junctions similar to Holliday junctions appear in some functional RNA molecules.Immobile Holliday junctions, with asymmetrical sequences that lock the strands in a specific position, were artificially created by scientists to study their structure as a model for natural Holliday junctions. These junctions also later found use as basic structural building blocks in DNA nanotechnology, where multiple Holliday junctions can be combined into specific designed geometries that provide molecules with a high degree of structural rigidity.