Diversity of DNA methyltransferases that recognize asymmetric
... hemimethylated DNA, and it therefore needs only a single methylation event to restore the fully methylated state. This is in consistent with the fact that most of the DNA MTases studied exist as monomers in solution. Multiple lines of evidence suggest that some DNA MTases function as dimers. Further ...
... hemimethylated DNA, and it therefore needs only a single methylation event to restore the fully methylated state. This is in consistent with the fact that most of the DNA MTases studied exist as monomers in solution. Multiple lines of evidence suggest that some DNA MTases function as dimers. Further ...
Agarose Gel Electrophoresis
... While this has a slight effect on the mobility of the DNA (Fig. 10.4.2D), it eliminates the need to stain the gel upon completion of the separation. An added advantage to running gels with ethidium bromide is that the mobility of the DNA can be monitored throughout the run until the desired separati ...
... While this has a slight effect on the mobility of the DNA (Fig. 10.4.2D), it eliminates the need to stain the gel upon completion of the separation. An added advantage to running gels with ethidium bromide is that the mobility of the DNA can be monitored throughout the run until the desired separati ...
Directionality in FLP Protein-promoted Site
... efficiency when a mutant site was reacted with an unaltered reaction is detected in this assay only if it occurs between DNA site, reactions involving two identical mutant sites exhibited molecules cut with different restriction enzymes. Reaction between nearly normal levels of recombination (4).Thi ...
... efficiency when a mutant site was reacted with an unaltered reaction is detected in this assay only if it occurs between DNA site, reactions involving two identical mutant sites exhibited molecules cut with different restriction enzymes. Reaction between nearly normal levels of recombination (4).Thi ...
Telomere maintenance without telomerase
... Although the majority of yeast cells die in the absence of telomerase, Lundblad and Blackburn (1993) observed that a small subpopulation could escape the lethal consequences of a telomerase defect. The rare survivors recovered from an est1-D strain not only regained the ability to grow but also disp ...
... Although the majority of yeast cells die in the absence of telomerase, Lundblad and Blackburn (1993) observed that a small subpopulation could escape the lethal consequences of a telomerase defect. The rare survivors recovered from an est1-D strain not only regained the ability to grow but also disp ...
5 DNA Replication
... home,‖ is whispered to a child, who runs to a second child and repeats the message. The message is relayed from child to child around the schoolyard until it returns to the original sender. Inevitably, the last child returns with an amazingly transformed message, such as ―Joe Brown has a pig living ...
... home,‖ is whispered to a child, who runs to a second child and repeats the message. The message is relayed from child to child around the schoolyard until it returns to the original sender. Inevitably, the last child returns with an amazingly transformed message, such as ―Joe Brown has a pig living ...
Inference of homologous recombination in bacteria using whole
... Figure 2. There are no instances of confidently inferred but incorrect recombination events in this example, with false-positive recombination intensity being limited to two types. Firstly, the edge of recombination region is sometimes imperfectly found (e.g. on branch 1 around 5200bp), and secondly ...
... Figure 2. There are no instances of confidently inferred but incorrect recombination events in this example, with false-positive recombination intensity being limited to two types. Firstly, the edge of recombination region is sometimes imperfectly found (e.g. on branch 1 around 5200bp), and secondly ...
DNA cloning
... Type II restriction enzymes are a single polypeptide aids in the purification of the enzyme for in vitro work. Second, Type II restriction enzymes recognize and cleave DNA at a specific sequence. The cleavage is on both strands of the DNA and results in a double-stranded break. Cleavage of the DNA l ...
... Type II restriction enzymes are a single polypeptide aids in the purification of the enzyme for in vitro work. Second, Type II restriction enzymes recognize and cleave DNA at a specific sequence. The cleavage is on both strands of the DNA and results in a double-stranded break. Cleavage of the DNA l ...
as PDF
... Agarose gel electrophoresis is commonly used to resolve circular DNA with different supercoiling topology, and to resolve fragments that differ due to DNA synthesis. DNA damage due to increased cross-linking proportionally reduces electrophoretic DNA migration (Blasiak et al., 2000; Lu & Morimoto, 2 ...
... Agarose gel electrophoresis is commonly used to resolve circular DNA with different supercoiling topology, and to resolve fragments that differ due to DNA synthesis. DNA damage due to increased cross-linking proportionally reduces electrophoretic DNA migration (Blasiak et al., 2000; Lu & Morimoto, 2 ...
Review A model for chromosome structure during the mitotic
... into a matrix strand during telophase. DNA loops (green lines ^ mostly 30-nm diameter chromatin ¢bers) are attached to the core/matrix strand via MARS (green dots) with closely associated replication origins (included in green dots). Probable attachments to transcription complexes are not illustrate ...
... into a matrix strand during telophase. DNA loops (green lines ^ mostly 30-nm diameter chromatin ¢bers) are attached to the core/matrix strand via MARS (green dots) with closely associated replication origins (included in green dots). Probable attachments to transcription complexes are not illustrate ...
10.1 - My Haiku
... Allele have a 50 percent chance of moving to a particular pole. The direction in which one bivalent aligns does not affect the alignment of other bivalents. Therefore different allele combinations should always be equally possible (if the gene loci are on different chromosomes – this does not hold f ...
... Allele have a 50 percent chance of moving to a particular pole. The direction in which one bivalent aligns does not affect the alignment of other bivalents. Therefore different allele combinations should always be equally possible (if the gene loci are on different chromosomes – this does not hold f ...
BIO450 Primer Design Tutorial
... which case you would aim for a different level of specificity. Primers are short single-stranded oligonucleotides (‘primers’) which hybridize (‘anneal’) to one strand of the target DNA (the ‘template’). Base-pairing complementarity leads to a short double-stranded region that serves as a site where ...
... which case you would aim for a different level of specificity. Primers are short single-stranded oligonucleotides (‘primers’) which hybridize (‘anneal’) to one strand of the target DNA (the ‘template’). Base-pairing complementarity leads to a short double-stranded region that serves as a site where ...
K - Romanian Biotechnological Letters
... The non-conventional yeast Kluyveromyces lactis has become an excellent alternative yeast model organism [1, 2]. Reconsidered to be a distinct species [3, 4], K. lactis is an ascomyceteous budding yeast that belongs to the endoascomycetales [1]. There are important reasons for the increased attracti ...
... The non-conventional yeast Kluyveromyces lactis has become an excellent alternative yeast model organism [1, 2]. Reconsidered to be a distinct species [3, 4], K. lactis is an ascomyceteous budding yeast that belongs to the endoascomycetales [1]. There are important reasons for the increased attracti ...
Clamp loader structure predicts the architecture of DNA polymerase
... accessible to β may be one in which the amino-terminal domain of δ′ interacts more extensively with the aminoterminal, β-interactive, domain of δ. Given very few modeling operations (explained in [17]) a hypothetical ‘closed’ structure can be formed in which the amino-terminal domains of all five su ...
... accessible to β may be one in which the amino-terminal domain of δ′ interacts more extensively with the aminoterminal, β-interactive, domain of δ. Given very few modeling operations (explained in [17]) a hypothetical ‘closed’ structure can be formed in which the amino-terminal domains of all five su ...
Extrachromosomal DNA Transformationof Caenorhabditis elegans
... amount of pBR322 hybridization expected for each worm DNA preparation if the plasmid sequences were present in one copy per genome (see Table 2). The percentage of transformed worms in each population was used to derive the copy number per genome per transformant. It should be noted that the copy nu ...
... amount of pBR322 hybridization expected for each worm DNA preparation if the plasmid sequences were present in one copy per genome (see Table 2). The percentage of transformed worms in each population was used to derive the copy number per genome per transformant. It should be noted that the copy nu ...
Generative Power and Closure Properties of Watson
... in fundamental way, to solve wide spectrum of computationally intractable problems. DNA (deoxyribonucleic acid) is double-stranded chain of nucleotides, which differ by their chemical bases that are adenine (A), guanine (G), cytosine (C), and thymine (T), and they are paired as A-T, C-G according to ...
... in fundamental way, to solve wide spectrum of computationally intractable problems. DNA (deoxyribonucleic acid) is double-stranded chain of nucleotides, which differ by their chemical bases that are adenine (A), guanine (G), cytosine (C), and thymine (T), and they are paired as A-T, C-G according to ...
Molecular events during translocation and proofreading extracted
... are difficult, if not impossible, to capture by static crystallography. These conformations are often key to mechanistic understanding and could be explored by a back calculation based on molecular distance geometry (12), the chief computational algorithm in nucleic magnetic resonance spectroscopy. ...
... are difficult, if not impossible, to capture by static crystallography. These conformations are often key to mechanistic understanding and could be explored by a back calculation based on molecular distance geometry (12), the chief computational algorithm in nucleic magnetic resonance spectroscopy. ...
Synapsis-Mediated Fusion of Free DNA Ends Forms Inverted Dimer Plasmids in Yeast.
... the plasmid do not share homology with the yeast genome, circular inverted (head-to-head) dimer plasmids are theprincipal product of repair. By measurements of the DNA concentration dependence of transformation with alinearized plasmid, and by transformation with mixtures of genetically marked plasm ...
... the plasmid do not share homology with the yeast genome, circular inverted (head-to-head) dimer plasmids are theprincipal product of repair. By measurements of the DNA concentration dependence of transformation with alinearized plasmid, and by transformation with mixtures of genetically marked plasm ...
Compaction of Duplex Nucleic Acids upon Native
... Figure S3), a rigid structure of the same size. This indicates that a greater conformational space is explored in the gas phase by nucleic acid duplexes compared to the G-quadruplex,14 and that gas-phase duplexes consist of an ensemble of conformations not fully interconverting on the time scale of ...
... Figure S3), a rigid structure of the same size. This indicates that a greater conformational space is explored in the gas phase by nucleic acid duplexes compared to the G-quadruplex,14 and that gas-phase duplexes consist of an ensemble of conformations not fully interconverting on the time scale of ...
A Mathematical Formulation of DNA Computation
... T , and vice versa. DNA computation involves to use singlestranded DNA segments to code the problem, let the singlestranded DNA segments react in test tubes or substrate surfaces, and then to find DNA binding strands and interpret the results by applying bio-molecular techniques. This process can be ...
... T , and vice versa. DNA computation involves to use singlestranded DNA segments to code the problem, let the singlestranded DNA segments react in test tubes or substrate surfaces, and then to find DNA binding strands and interpret the results by applying bio-molecular techniques. This process can be ...
Modified PDF
... DNA structure. The collaboration between pol and DBP is not understood in detail but it is specific, suggesting an interaction between the two proteins. Secondly, DBP can unwind short stretches of dsDNA or even longer stretches if short single-stranded protruding ends are present (Georgaki et al. 19 ...
... DNA structure. The collaboration between pol and DBP is not understood in detail but it is specific, suggesting an interaction between the two proteins. Secondly, DBP can unwind short stretches of dsDNA or even longer stretches if short single-stranded protruding ends are present (Georgaki et al. 19 ...
lecture 1 File
... specific proteins. This concept proved to be the opening gun in what becamemolecular genetics and all the developments that have followed from that.[19] Saccharomyces cerevisiae is a yeast of the phylum Ascomycota. During vegetative growth that ordinarily occurs when nutrients are abundant, S. ...
... specific proteins. This concept proved to be the opening gun in what becamemolecular genetics and all the developments that have followed from that.[19] Saccharomyces cerevisiae is a yeast of the phylum Ascomycota. During vegetative growth that ordinarily occurs when nutrients are abundant, S. ...
Principles of Nucleic Acid Separation by Agarose Gel Electrophoresis
... Agarose gel electrophoresis is commonly used to resolve circular DNA with different supercoiling topology, and to resolve fragments that differ due to DNA synthesis. DNA damage due to increased cross-linking proportionally reduces electrophoretic DNA migration (Blasiak et al., 2000; Lu & Morimoto, 2 ...
... Agarose gel electrophoresis is commonly used to resolve circular DNA with different supercoiling topology, and to resolve fragments that differ due to DNA synthesis. DNA damage due to increased cross-linking proportionally reduces electrophoretic DNA migration (Blasiak et al., 2000; Lu & Morimoto, 2 ...
Gel Electrophoresis - Integrated DNA Technologies
... Polyacrylamide gel is the result of polymerizing acrylamide monomers into long chains and then cross-linking the chains with a bifunctional compound. A number of these bifunctional cross-linkering compounds are known including ethylene diacrylate, N,N’bisacrylycystamine (BAC), and N,N’-diallyltartar ...
... Polyacrylamide gel is the result of polymerizing acrylamide monomers into long chains and then cross-linking the chains with a bifunctional compound. A number of these bifunctional cross-linkering compounds are known including ethylene diacrylate, N,N’bisacrylycystamine (BAC), and N,N’-diallyltartar ...
SNP-Based Mapping of Crossover Recombination in
... and provide a dense array of potential genetic markers for use in measurement of recombination. These markers have the advan tage of being phenotypically neutral (in general) and codominant, thus avoiding potential complications due to viability and sim plifying scoring. In addition, multiple mark ...
... and provide a dense array of potential genetic markers for use in measurement of recombination. These markers have the advan tage of being phenotypically neutral (in general) and codominant, thus avoiding potential complications due to viability and sim plifying scoring. In addition, multiple mark ...
Repair of Site-Specific DNA Double-Strand Breaks in
... throughout the cell cycle, but predominantly occur during G1, when sister chromatids are not available (reviewed in Lieber, 2010). Moreover, both ends of a DSB may interact separately and follow different repair pathways, which result in various types (and combinations) of rearrangements. A nonrecip ...
... throughout the cell cycle, but predominantly occur during G1, when sister chromatids are not available (reviewed in Lieber, 2010). Moreover, both ends of a DSB may interact separately and follow different repair pathways, which result in various types (and combinations) of rearrangements. A nonrecip ...
Holliday junction
A Holliday junction is a branched nucleic acid structure that contains four double-stranded arms joined together. These arms may adopt one of several conformations depending on buffer salt concentrations and the sequence of nucleobases closest to the junction. The structure is named after the molecular biologist Robin Holliday, who proposed its existence in 1964.In biology, Holliday junctions are a key intermediate in many types of genetic recombination, as well as in double-strand break repair. These junctions usually have a symmetrical sequence and are thus mobile, meaning that the four individual arms may slide though the junction in a specific pattern that largely preserves base pairing. Additionally, four-arm junctions similar to Holliday junctions appear in some functional RNA molecules.Immobile Holliday junctions, with asymmetrical sequences that lock the strands in a specific position, were artificially created by scientists to study their structure as a model for natural Holliday junctions. These junctions also later found use as basic structural building blocks in DNA nanotechnology, where multiple Holliday junctions can be combined into specific designed geometries that provide molecules with a high degree of structural rigidity.