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Tutorial: RNA-Seq Analysis Part II (Tracks): Non-Specific
Tutorial: RNA-Seq Analysis Part II (Tracks): Non-Specific

... Figure 10: The dot in the scatter plot corresponding to the gene "Rps13" and the corresponding region in the trackm list "zoomed-to-selection". area showing the visible reads by grabbing at the bottom of the track's editor and dragging it downwards. You will get a view similar to that in figure 11. ...
GeneCensus - Gerstein Lab Publications
GeneCensus - Gerstein Lab Publications

... We present a prototype of a new database tool, GeneCensus, which focuses on comparing genomes globally, in terms of the collective properties of many genes, rather than in terms of the attributes of a single gene (e.g. sequence similarity for a particular ortholog). The comparisons are presented in ...
Measurement of the synthesis of liver
Measurement of the synthesis of liver

... radioactive amino acid, and ["Slmethionine and [35S]cystine were used for such studies. Problems arose because net plasma protein synthesis accounted for only a small part of the amino acid incorporation, and breakdown of more rapidly turning over proteins released amino acids which could be reutili ...
(CH11) Transcription In Eukaryotes (Slides)
(CH11) Transcription In Eukaryotes (Slides)

... • Approximately 60 bp DNA sequence overlapping the transcription start site. • Serves as the recognition site for RNA pol II and the general transcription factors. • All core promoter elements, except for BRE, are recognized by TFIID. • A particular core promoter may contain some, all, or none of th ...
Genetics Core - Alzheimer`s Association
Genetics Core - Alzheimer`s Association

... 2013: 1st whole-exome sequencing study in MCI (1st extreme MRI phenotype in MCI) 2013: Demonstrated strong influence of genetic variation on plasma protein levels 2013: 1st large scale WGS data set released to scientific community – analyses begin 2013: 1st GWAS of the healthy human structural conne ...
Prof. Gil Segal Research
Prof. Gil Segal Research

... effect on yeast growth suggests that a conserved and essential eukaryotic process, which is modulated by the effectors in the host cell, was also modulated in the yeast cell, resulting in an inhibition of yeast cell growth. In addition, effectors sometimes target a cellular pathway that is conserved ...
Gene Section EIF3A (eukaryotic translation initiation factor 3, subunit A)
Gene Section EIF3A (eukaryotic translation initiation factor 3, subunit A)

... (Pincheira et al., 2001b). It has phosphorylation sites at Ser-881, Ser-1198, Ser-1336 and Ser-1364 (Damoc et al., 2007). The PCI domain spans from amino acid 405 to 495, which contains purely alpha-helix (Pincheira et al., 2001b). Since most of the proteins containing this domain are part of a mult ...
Nerve activates contraction
Nerve activates contraction

... • The presence of introns, long non-coding regions, in eukaryotic genes creates problems for expressing these genes in bacteria. • To express eukaryotic genes in bacteria, a fully processed mRNA acts as the template for the synthesis of a complementary strand using reverse transcriptase. • This com ...
A unique segment of the hepatitis B virus group A genotype
A unique segment of the hepatitis B virus group A genotype

... additional three PCR amplicons lacking either the preS2 region (one specimen) or part of the S gene (two specimens) were also successfully genotyped. The degree of diversity (Table 2) of the preS2 region at both the nucleotide and amino acid levels is greater than that of the S region. When the preS ...
BCOR 101, Exam 1 Be sure to show your work!. 1 Name ____KEY
BCOR 101, Exam 1 Be sure to show your work!. 1 Name ____KEY

... Chloroplasts are maternally inherited through the cytoplasm of the ovule. Because the seeds are produced on a white branch, from white ovules, All offspring will have white leaves. See p 56-7 and Fig 2.31 -orb. By what mechanism do chromosomes separate during mitosis or meiosis (include the word “ki ...
AAV8-hFIX19 Center for Cellular and Molecular Therapeutics at
AAV8-hFIX19 Center for Cellular and Molecular Therapeutics at

... of genetically modified organisms On the basis of an environmental risk assessment carried out in accordance with the principles and methodology outlined in parts B and C of Schedule II SI 500/2003, the information on the points listed in parts D1 of this schedule is provided with a view to assistin ...
Transcription in Eukaryotes
Transcription in Eukaryotes

... • Approximately 60 bp DNA sequence overlapping the transcription start site. • Serves as the recognition site for RNA pol II and the general transcription factors. • All core promoter elements, except for BRE, are recognized by TFIID. • A particular core promoter many contain some, all, or none of t ...
Is it Time to Get on the Fast Track or Stay on the
Is it Time to Get on the Fast Track or Stay on the

... intravenous MS in relieving experimental ischemic pain,7 Lötsch et al. found that 0.045 mg/kg of intravenous M6G (followed by an infusion to maintain steadystate plasma concentrations) was ineffective in blunting the pain associated with nasal insufflation of 60% CO2.8 In the present study, Romberg ...
Meiosis ppt
Meiosis ppt

... sperm cells. They lose most of their cytoplasm, remodel their cell shape, and grow a long flagellum (tail). ...
Intro (15min): finish Kahoots Activity #1 (30min): Short Answer
Intro (15min): finish Kahoots Activity #1 (30min): Short Answer

... phenotype  from  which  of  the  following  individuals:  …?   • Answer  the  question:  “A  white-­‐eyed  female  Drosphilia  is  mated  with  a  red-­‐ eyed  (wild-­‐type)  male.  What  results  do  you  predict  for  the  numerous   offs ...
Extralenticular expression of Xenopus laevis alpha-, beta
Extralenticular expression of Xenopus laevis alpha-, beta

... Xenopus laevis embryos were obtained from the Depart- amino acids. Sequence comparison revealed that ment of Zoology of the University of Nijmegen or the this protein is the X. laevis homologue of /3A4-crysHubrecht Laboratory in Utrecht and were maintained tallin. The deduced amino acid sequence sho ...
ap15-ChromosomalBasisofInheritance 07-2008
ap15-ChromosomalBasisofInheritance 07-2008

... • The difference between the disorders is due to genomic imprinting. • In this process, a gene on one homologous chromosome is silenced, while its allele on the homologous chromosome is expressed. • The imprinting status of a given gene depends on whether the gene resides in a female or a male. – T ...
No Slide Title
No Slide Title

... Catecholamine synthesis and release are two separate but related processes that influence the level of catecholaminergic neurotransmission. Neuropeptide Y (NPY) is a 36 amino acid peptide that is highly homologous to peptide YY (PYY). NPY exerts its various biological effects through at least six cl ...
Evolution of Development
Evolution of Development

... Some of the best examples of this approach come from studies addressing the origin of animal appendages. The developmental regulatory gene, distal-less, was one of the first genes to be examined in this context. Distal-less protein is a transcription factor that plays an important role in organizing ...
Telomerase Is a True Reverse Transcriptase
Telomerase Is a True Reverse Transcriptase

transcription factor
transcription factor

... • Approximately 60 bp DNA sequence overlapping the transcription start site. • Serves as the recognition site for RNA pol II and the general transcription factors. • All core promoter elements, except for BRE, are recognized by TFIID. • A particular core promoter many contain some, all, or none of t ...
General remarks: A biodesign is a man made, mostly computer
General remarks: A biodesign is a man made, mostly computer

... customer’s requirements for the biological or other like technical functionality of the DNAs or its gene products of interest. The results for this assessment of the best mode for the biodesign of gene variants are presented to the customer in a detailed report and discussed before starting the real ...
haemoglobin: structure, properties and biomedical functions
haemoglobin: structure, properties and biomedical functions

... Hb is largely alpha-helical; each chain contains helical segments between which are short non coiled segments. The chains are wound round itself to form a pocket in which the heme group nestles and this pocket is usually formed by hydrophobic amino acids. ...
Partners in time. Current Biology 6, 244-246. pdf
Partners in time. Current Biology 6, 244-246. pdf

... fragments of the Tim protein; experiments with glutathione-S-transferase fusion proteins in vitro confirmed a direct biochemical interaction between the Per and Tim proteins. These authors also showed that the per L mutation — a missense mutation affecting the PAS domain that lengthens the circa dia ...
Duchenne Muscular Dystrophy
Duchenne Muscular Dystrophy

... Mental development is impaired. IQ’s usually below 75 points.  Memory problems  Carrying out daily functions ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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