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The Role of Oocyte‐Secreted Factors GDF9 and BMP15 in Follicular
The Role of Oocyte‐Secreted Factors GDF9 and BMP15 in Follicular

... and Shimasaki 2002). Unlike GDF9, BMP15 protein is found in the pituitary gland, testis and in several other tissues from many species, suggesting that BMP15 activity is not exclusive to the ovary (Otsuka and Shimasaki 2002). BMP15 mRNA was not detected in oocytes of primordial stage follicles and i ...
RNA Tertiary Structure
RNA Tertiary Structure

... Ninety eight percent of the human genome does not code for protein. What is its function? ...
GLYPHOSATE RESISTANCE Background / Problem
GLYPHOSATE RESISTANCE Background / Problem

...  So far, only considering dynamics of alleles at single loci  Loci occur on chromosomes, linked to other loci! “The fitness of a single locus ripped from its interactive context is about as relevant to real problems of evolutionary genetics as the study of the psychology of individuals isolated fr ...
What is cell
What is cell

...  Addresses a high unmet need in cancer by enabling easier, more frequent monitoring of emergence of oncogene mutation status, disease progression and recurrence ...
Supplementary Notes - Word file
Supplementary Notes - Word file

... replaced with a fragment derived from pGLf37-CM1 digested with the same enzymes to construct pTVLtS286N. method ...
Rationale for Net and Absolute PI scores Pathway/processes may
Rationale for Net and Absolute PI scores Pathway/processes may

... Pathway/processes may contain both “activators” and “inhibitors” of the biological endpoint. Therefore, it is of interest to determine whether or not perturbing such a pathway will tend towards an inhibitory or an activation effect. For this reason a net score is calculated. We use the absolute scor ...
34. Measuring Selection in RNA molecules.
34. Measuring Selection in RNA molecules.

... a 4*4 matrix of the four bases, where each entry records the number of times a base changes into another base. Alternatively, it could be a doublet, participates in stem structures and results in a 16*16 matrix. Each entry records the count of base pairs changing into another base pair. Finally, it  ...
Cloning and characterization of the Xenopus laevis p8 gene
Cloning and characterization of the Xenopus laevis p8 gene

... (Fig. 2c) and, given that Xp8 is localized to the nucleus, it is likely to be a transcription factor. The DNAbinding activity of human p8 is increased with phosphorylation (Encinar et al. 2001) and, while we could not identify any putative phosphorylation sites on Xp8, there were a couple of well-co ...
ELMER: An R/Bioconductor Tool Inferring Regulatory Element
ELMER: An R/Bioconductor Tool Inferring Regulatory Element

... capture changes in individual molecular subtypes occurring in 20% or more of the cases. This number can be set arbitrarily as an input to the get.diff.meth function in the ELMER, and should be tuned based on sample sizes in individual studies. The one tailed t-test was used to rule out the null hyp ...
Members of the RKD transcription factor family induce an egg cell
Members of the RKD transcription factor family induce an egg cell

... on the notion that most cDNA libraries are made from tissues or plant organs in which egg cells and their transcripts are highly diluted or not present, the analysis was focused on 125 unique sequences which did not show any significant sequence similarity to more than one million publicly available ...
embracing - Iowa State University
embracing - Iowa State University

Genes & Inheritance Series: Set 3 Copyright © 2005 Version: 2.0
Genes & Inheritance Series: Set 3 Copyright © 2005 Version: 2.0

... optimum activity for each enzyme. This is because the active sites of the enzyme can be disabled by the wrong pH. ...
Analyses of human–chimpanzee orthologous gene
Analyses of human–chimpanzee orthologous gene

... GenAge’s 242 protein-coding genes, 211 could be correctly analyzed. Similarly, of GenAge’s 242 protein-coding genes, we obtained 169 human–rhesus, 216 human–mouse, and 205 human–rat gene pairs that could be correctly analyzed. Therefore, genes of potential interest may have been left out of our anal ...
Modifiers of epigenetic reprogramming show paternal effects in the mouse
Modifiers of epigenetic reprogramming show paternal effects in the mouse

Topic guide 12.4: Analysis methods
Topic guide 12.4: Analysis methods

... Determining the statistical significance of results returned by such analysis methods will get harder as the onslaught from next- and third-generation sequencing technologies gains pace. Moreover, determining statistical significance does not guarantee biological significance; rigorous checks should ...
PATTERNS OF INHERITANCE
PATTERNS OF INHERITANCE

... Trinucleotide CAG repeats over 28 are unstable during replication and this instability increases with the number of repeats present.[15] This usually leads to new expansions as generations pass (dynamic mutations) instead of reproducing an exact copy of the trinucleotide repeat.[13] This causes the ...
v2 PEBiosystems News 2 01/00
v2 PEBiosystems News 2 01/00

... sequence was a major scientific accomplishment, but the application of this genomic information to the understanding of biology and disease will have the greatest potential to impact medicine. In this phase of discovery, life science researchers will need the ability to transform the 3.2 Gb of raw s ...
Narayanasamy Research
Narayanasamy Research

... M. Kurosu, P. Narayanasamy, K. Biswas, R. Dhiman, and D. C. Crick. (2007). Discovery of 1, 4dihydroxy-2-naphthoate prenyl transferase inhibitors: New drug leads for Multidrug-Resistant gram-positive pathogens. Journal of Medicinal Chemistry, 3973-3975. d) M. Kurosu, S. Mahapatra, P. Narayanasamy, an ...
Review Article The biosafety of molecular farming in plants
Review Article The biosafety of molecular farming in plants

... harvested and processed using existing agricultural infrastructure and unskilled labour. Furthermore, while scaling up production in fermenter-based systems and transgenic animal herds is expensive and limited in scope, production systems based on plants can be scaled up and down simply by increasin ...
Regulated Lentiviral Packaging Cell Line Devoid of Most Viral cis
Regulated Lentiviral Packaging Cell Line Devoid of Most Viral cis

20.GeneticsSpg08 - Napa Valley College
20.GeneticsSpg08 - Napa Valley College

... A linear stretch of DNA is reversed within the chromosome ...
Warren, ST and Nelson, DL: Trinucleotide repeat expansions in neurological disease. Current Opinion in Neurobiology 3:752-759 (1993).
Warren, ST and Nelson, DL: Trinucleotide repeat expansions in neurological disease. Current Opinion in Neurobiology 3:752-759 (1993).

... For decades, mutational mechanisms that lead to human genetic disease have followed rules and examples set forth in model systems such as Drosophila and yeast. In recent years, however, new mechanisms responsible for genetic disease have emerged where little or no precedent had been established in o ...
Slides - Indico
Slides - Indico

On the Evolution of Primitive Genetic Codes
On the Evolution of Primitive Genetic Codes

... In general, however, such changes will be deleterious because every protein that contains the modified codon will be affected. In recent years three mechanisms of codon changes especially in mitochondria were published and each of them predicts certain codon changes that have not yet been observed. ...
Document
Document

... involved into formation of the whole organism body. •Motor proteins. These proteins can convert chemical energy into mechanical energy. actin and myosin are responsible for muscular motion. •Receptors These proteins are responsible for signal detection and translation into other type of signal. •Sig ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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