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By Lydia Bosman, MSc (Agric), and Rulien Grobler, BSc (Agric),
By Lydia Bosman, MSc (Agric), and Rulien Grobler, BSc (Agric),

... to give a cremello coat. These horses are livestock is by no means a simple characterised by a pink skin and blue process and with recent molecular eyes. Other loci that can dilute the base research available, much more colours include dun, champagne and complex as previously known. Our silver dappl ...
The Mobile Genetic Element Alu in the Human Genome
The Mobile Genetic Element Alu in the Human Genome

... genome mapping and biomedical research. Only a small fraction of the human genome consists of functional, or protein-encoding, genes. If all the nucleotide information were translated, 10 9 amino acids, or approximately 3 million average-size proteins, would be encoded. Based on the fully sequenced ...
Full Version - TS
Full Version - TS

... shelf, nowadays it is necessary for a drug to pass through a hierarchically structured series of tests, to prove its efficacy and safety. The use of laboratory animals in scientific experiments is an important matter of debate and needs to be debated, but the fact that science currently depends on a ...
Looking at long molecules in solution: what happens when they are
Looking at long molecules in solution: what happens when they are

... One of the challenges of working with high order macromolecular complexes, e.g. protein fibres or carbon nanotubes, is that they scatter light. The lenses of the microvolume capillary LD cells reduce this effect but it is still an issue, see e.g. ref. 38 and 39. The problem arises because the spectrom ...
Lecture 8
Lecture 8

... states, and in most, if not all, industrialized countries (called the Guthrie Test) Test blood for phenylalanine, and if positive, do further tests Impetus behind the testing is that PKU is preventable Issue of mandatory genetic testing: there is a recommendation against genetic testing at birth for ...
1 ESHG/ESHRE Responsible Innovation in Human Germ
1 ESHG/ESHRE Responsible Innovation in Human Germ

... propagated as clones in culture and then transplanted back into the testis to generate mature and functional sperm (Goosens et al., 2013). So a potential strategy would be to select SSC clones which have undergone correct genomic editing and are free from off-target mutations. These can then be tran ...
Public‐private partnerships in plant‐breeding research
Public‐private partnerships in plant‐breeding research

... Role of Pre‐breeding in the plant  breeding development chain • To stimulate innovation transfer at the farm level ;  – To ensure innovation transfer to elite material – To translate academic knowledge in workable tools, adapted  materials  • To be able to introduce primary diversity (eg. a necessit ...
Another five years! Year of Biodiversity photography competition
Another five years! Year of Biodiversity photography competition

... years of continuous support for TEDS. Typical of the many glowing reviews was this one: ‘No other study internationally has been as influential in the field’. Many thanks to you for making this award possible by your help during the past 15 years. This new MRC award will allow us to invite all TEDS ...
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Genetics

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Nucleotide Sequence of fruA, the Gene Specifying Enzyme IIfru of
Nucleotide Sequence of fruA, the Gene Specifying Enzyme IIfru of

Journal of Applied Ecology
Journal of Applied Ecology

... assessed by 16S rRNA-based terminal-restriction fragment length polymorphism (T-RFLP) analysis. Cultivated community members were identified by 16S rRNA gene analysis and screened for a range of plant growth-promoting and plant pathogenantagonistic abilities. 3. Both genetic transformation events in ...
Solving Even-Parity Problems using Multi Expression Programming
Solving Even-Parity Problems using Multi Expression Programming

... and even-4 parity problems using a population of 4000 individuals [3]. The cumulative probability of success was 100% for the even-3-parity problem and 42% for the even-4-parity problem [3]. A perfect comparison between MEP and GP cannot be made due to the incompatibility of the respective represent ...
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... After they are synthesized, most proteins fold into compact, three-dimensional shapes. Folding is an essential step in the synthesis of a protein because proteins can perform their functions only if they can bind to other molecules. To do so, they must have specific binding sites within their struct ...
Root organization and gene expression patterns
Root organization and gene expression patterns

... In pea roots, the vascular cylinder can be subdivided genetics are making it possible for biologists to study into xylem and phloem sectors (Rost et al., 1988). Xylem roots with new vigour. Such investigations have pericycle cells continue to divide in a transverse plane enabled plant biologists to ...
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Changes in DNA-binding activity of transcription factors in the

biomass composition
biomass composition

... Modeling Lactococcus lactis using a genome-scale flux model ...
Full-Text PDF
Full-Text PDF

... One strategy for forward genetic screens has been to look for distorted segregation of mutagen-encoded marker phenotypes, such as T-DNA encoded antibiotic resistance, to identify plants with reduced transmission of marker phenotypes [6,7]. Forward genetic screens have also targeted mutants exhibitin ...
Identification of a major gene in F1 and F2 data when alleles
Identification of a major gene in F1 and F2 data when alleles

... (1000 observations) the likelihood ratio test was conservative and yielded a type I error of 3%, at a nominal level of 5%. The power of the test reached > 95% for additive and completely dominant effects of 4 and 2 residual SDs respectively. For smaller data sets, power decreased. In this model assu ...
The Art of Multiple Sequence Alignment in R - decipher
The Art of Multiple Sequence Alignment in R - decipher

... alignment. The idea is to give the alignment a biological basis even though the molecules that the sequences represent will never meet each other and align under any natural circumstance. The workhorse for sequence alignment in DECIPHER is AlignProfiles, which takes in two aligned sets of DNA, RNA, ...
Biological monomers and polymers (1)
Biological monomers and polymers (1)

... on the planet. There are some exceptions in which a few of the codons have different meanings. Thus, the information to arrange aminoacids in a specific sequence with a particular function is coded in a sequence of nucleotides in DNA. The process and the machinery that generates a protein from a DNA ...
Heavy Chain Diversity Region Segments of the Channel Catfish
Heavy Chain Diversity Region Segments of the Channel Catfish

LAB 5: Breeding Bunnies - Ms Kim`s Biology Class
LAB 5: Breeding Bunnies - Ms Kim`s Biology Class

... Sometimes the frequency of alleles changes in a population over a period of time. This means that how often you will see a particular trait will change also. The purpose of this experiment is to model the changes in gene frequency over several generations. In this lab, the dominant allele for normal ...
Regulation of DNA Polymerase Exonucleolytic Proofreading Activity
Regulation of DNA Polymerase Exonucleolytic Proofreading Activity

... proofreading, which removes correct nucleotides in addition to incorrect nucleotides (Muzyczka et al. 1972; Gillin and Nossal, 1976a; reviewed in Goodman et al. 1993). Another potential disadvantage of increased DNA replication accuracy is the possible necessity of a certain minimal mutation rate th ...
PDF - Oxford Academic - Oxford University Press
PDF - Oxford Academic - Oxford University Press

... vertebrates for many disorders, from congenital to multifactorial diseases such as cardiovascular, neurodegenerative, metabolic and cancer diseases (e.g. [3]). Indeed, 75–80% of human genes associated with disease—either annotated in OMIM or identified in GWAS—have at least one zebrafish orthologue, ...
Protocol S1
Protocol S1

... Equation (S1) gives the expected number of generations until two beneficial mutations arepresent together in the same individual. Consequently, 31  g generations must pass, on average, until an individual would arise that had lost 32 chromosomes by mutation, if each mutation were to occur indepen ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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