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Slide 1
Slide 1

... • DNA Microarrays (for chemical analysis) • Protein Sensors (for identifying viruses) ...
2. Snuffle Snork
2. Snuffle Snork

... During one of my recent excursions to the wilds of Schmidlandia, I discovered a previously unknown creature. I have named this new life form Snorkus schmiddicus, or “Snork” for short. I was able to capture four different individuals and collect a sample of their DNA. They were then released unharmed ...
Document
Document

... searching for proteins with the keyword “cyclin.” A BLAST search with a cyclin protein sequence ensured that the cyclin gene was identified using this method. Microarray data during conjugation (Miao et al., 2009) was collected for the gene from the Tetrahymena Gene Expression Database (TGED; http:/ ...
Transduction
Transduction

DNA
DNA

... method of separating molecules based on their charge, size, and shape. It is especially useful in separating charged molecules of DNA and RNA. [When an electric current is applied to the gel, negatively charged molecules move toward the positive end, and positively charged molecules move toward the ...
PattArAn – From Annotation Triplets to Sentence Fingerprints
PattArAn – From Annotation Triplets to Sentence Fingerprints

Frontiers in medical genetics: Advancing understanding in heritable
Frontiers in medical genetics: Advancing understanding in heritable

III) Basic manipulations
III) Basic manipulations

... a) We will transform this strain with a library. This library will be random insertions of genomic DNA from a wildtype strain that have been cloned into a vector. You could actually either select or screen for the cells that are rescued because they contain the plasmid containing a wildtype version ...
Experimental General. All the DNA manipulations and bacterial
Experimental General. All the DNA manipulations and bacterial

... Together with the above mutagenic primers, in the first PCRs, BC-LIP-9F (5’CCGCCACGTACAACCAGAACTATC-3’) and PET-2R (5’-GTTATTGCTCAGCGGTGG3’) were also used, and in the second PCR, BC-LIP-9F and PET-2R were used. The conditions for the 100 µL PCR mixture were as follows: 0.5 µM each primer, 0.2 mM ea ...
genome - Microme
genome - Microme

... TrEMBL contains functional annotations which often come from automatic procedures only: ‘IPMed?’ is used for proteins that may have an experimentally validated function. ...
annotation transcriptomics doc
annotation transcriptomics doc

... ESTTik consists in a main program that uses Perl modules. Using these programs, the pipeline analyzes raw input data in order to clean the sequences (low quality or too short sequences, etc). The sequences are then assembled in a non-redundant set of data (consensus and singletons). Afterward, the p ...
The Excitement of Biochemical Engineering
The Excitement of Biochemical Engineering

... One of the earliest challenges we faced was to address a potential threat to the large scale processing of proteins which would have made the preparation of enough material for patients extremely difficult. These are very complex molecules as the simulated image of one of the smaller catalytic prote ...
Biomolecules Cut n Paste Slides
Biomolecules Cut n Paste Slides

... called nucleotides. Nucleotides consist of three parts: a 5-carbon sugar; a phosphate group; and a nitrogenous base. Nucleic acids store and transmit hereditary or genetic information. There are two kinds of nucleic acids: ribonucleic acid (RNA) which is single stranded and deoxyribonucleic acid (DN ...
New RCSI research demonstrates how cannabis use during
New RCSI research demonstrates how cannabis use during

... gene, called the COMT gene, to cause physical changes in the brain. The COMT gene provides instructions for making enzymes which breakdown a specific chemical messenger called dopamine. Dopamine is a neurotransmitter that helps conduct signals from one nerve cell to another, particularly in the brai ...
Synthetic Nucleic Acids
Synthetic Nucleic Acids

... quantified by genome copy number using Droplet Digital™ PCR, and produced under ISO 9001:2008 certified as well as ISO/IEC 17025:2005 and ISO 13485:2003 accredited processes, so you can trust the accuracy of your results. What’s more, each DNA or RNA preparation is stabilized using a DNA- or RNAbase ...
Caffeine Metabolism Gene Zephyr and Walsh (2015)
Caffeine Metabolism Gene Zephyr and Walsh (2015)

... asked to identify the restriction sites for both ApaI and SacI, describe the sizes that will occur when the enzymes do or do not cut, and then draw out the gels for homozygotes and a heterozygote. In this way, students can form an educated guess about the variety of results they may achieve and make ...
e results depicted in Figure 110 suggested that 2.5 Results
e results depicted in Figure 110 suggested that 2.5 Results

Extended Methods
Extended Methods

... generate the SMN-IS plasmids, while RBex13F and RBIS13 primers3 were used to obtain RB-IS plasmids. The SMN-IS and RB-IS are plasmids with SMN and RB1 inserts equal to the genomic amplicons but having internal deletions of 11 and 19 bp respectively. Both ISs are amplified by the same primers as thei ...
Mutations PP
Mutations PP

... Some mutations are silent or neutral Chemicals and UV radiation causes mutations (mutagens) Many mutations are repaired by enzymes Some types of skin cancers and leukemia result from somatic mutations Some mutations may improve an organism’s survival (beneficial) Most changes in DNA are not benefici ...
DNA Extraction from Plant and Animal Cells
DNA Extraction from Plant and Animal Cells

... My observations are consistent with my hypothesis. More DNA was extracted from plant cell samples treated with cellulase than those treated without. This is due to the action of the enzyme cellulase in breaking down the cellulose of plant cell walls. The amount of DNA extracted from animal cells dep ...
Fact Sheet 10 | X-LINKED DOMINANT INHERITANCE This fact
Fact Sheet 10 | X-LINKED DOMINANT INHERITANCE This fact

... An X-linked dominant gene is a gene located on the X chromosome and effects males and females differently. ...
Our system for annotation of articles is named “Text
Our system for annotation of articles is named “Text

... annotating a wide range of biological entities, such as genes, proteins, chemical compounds, drugs, diseases, biological processes, etc. For the purposes of BioCreative, this document only refers to the gene annotation machinery. ...
Genetics
Genetics

... and electricity is run through it • A standard with known DNA sizes is placed in at least one well to compare • The different sized fragments (measured in number of base pairs) stop at certain points and the unknown is compared with the known samples ...
Plankton of Bamfield Inlet
Plankton of Bamfield Inlet

... pieces of DNA of known size that you can compare against your migrating DNA. As your DNA migrates through the gel, the loading dye becomes diluted and will no longer be visible. The finished gel must be stained before we can see your DNA. To stain DNA, your gel will be soaked for 10 minutes in 5% et ...
Ch6Sec4 Reiforce Tratis Genes Alleles
Ch6Sec4 Reiforce Tratis Genes Alleles

... polypeptide. The location of a gene on a chromosome is called a locus. A gene has the same locus on both chromosomes in a pair of homologous chromosomes. In genetics, scientists often focus on a single gene or set of genes. Genotype typically refers to the genetic makeup of a particular set of genes ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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