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heredity (b)
heredity (b)

... DNA replication is crucial to the advancement of a cell in the cell cycle. In what stage does a cells chromosomal DNA go through replication? S - phase What is the principal enzyme that is responsible for DNA replication? DNA Polymerase (III) DNA replication is directional. In what direction does it ...
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No Slide Title

... within the replication fork, called primosome. DNA pol3 - a dimer - one set of subunits synthesize the leading strand and other set the lagging strand. Once DNA is unwound by DnaB, DnaG associates occasionally with DnaB and synthesizes a short RNA primer. A new  sliding clamp is then positioned at ...
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the study of complex vertebral malformation genetic defect in a

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protein synthesis overview

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No Slide Title

... P. aurelia does not really exist as a species any longer since Sonneborn (1975) divided the aurela complex into 14 syngens = species. A species, following Mayr (1957), is a sexually isolated population. It cannot cross with any others. How different species are morphometrically, or in their DNA or ...
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Supplementary Information (doc 28K)

... siRNA (Cy). After 24 hours cells were incubated in the presence or absence of hypoxic condition (1%O2, 5%CO2 atmosphere or CoCl2 treatment) for 12 hours. HIF-1 mRNA expression was evaluated by RT-PCR whereas HIF-1 protein level and activity was detected by western blot and ELISA, respectively, as ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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