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What is a gene, post-ENCODE? History and updated definition
What is a gene, post-ENCODE? History and updated definition

... It was the solution of the three-dimensional structure of DNA by Watson and Crick in 1953 (Watson and Crick 1953) that explained how DNA could function as the molecule of heredity. Base pairing explained how genetic information could be copied, and the existence of two strands explained how occasion ...
DNA and Genetics
DNA and Genetics

... It is envisaged that the delivery of Outcome 1 could commence with a recap on DNA structure and replication. Simple DNA extraction could be performed as an introduction to the Unit. The stages of the cell cycle should then be covered. In the teaching of the cell cycle, the importance of the varying ...
DNA to Protein
DNA to Protein

... are called proteases – process is called proteolysis In the cytosol there are large complexes of proteolytic enzymes that remove damaged proteins Ubiquitin, small protein, is added as a tag for disposal of protein ...
recombinant DNA
recombinant DNA

... by faulty genes. Recombinant DNA (rDNA) technology (genetic engineering) offers a new way of combating these hereditary diseases by either replacing the faulty genes or producing the missing peptides and proteins so that they can be given as a medicine (see section 10.15.2). prof. aza ...
Chapter 4
Chapter 4

... A metabolic pathway is a sequence of enzyme-controlled reactions required to convert substances into useable forms. These pathways are interconnected so that substances can be catabolized or anabolized per the needs of the cells at that particular time. 18. Describe the starting material and product ...
The Scripps Research Institute
The Scripps Research Institute

Topic 10: « MODERN METHODS OF DNA DIAGNOSIS OF
Topic 10: « MODERN METHODS OF DNA DIAGNOSIS OF

... called DNA polymerase. This enzyme makes the complementary strand by finding the correct base through complementary base pairing, and bonding it onto the original strand. As DNA polymerases can only extend a DNA strand in a 5′ to 3′ direction, different mechanisms are used to copy the antiparallel str ...
answers to exam 2011 - Learning on the Loop
answers to exam 2011 - Learning on the Loop

... Meiosis produces gametes with half the number of chromosomes. This means that pairs of alleles are separated at meiosis. At fertilisation, which sperm fertilise which egg is due to chance and this results in new combinations of alleles. The advantage of variation to a species is that it may enable s ...
Additional file - Supplementary material
Additional file - Supplementary material

... genes which were DE in the RNA-seq data, using both the GOseq and hypergeometric methods. By taking the microarray GO analysis as our gold standard, we compared the ability of GOseq and existing methods to reproduce these results. We plot the fraction of recovered microarray GO categories as a func ...
THE DEVELOPMENT OF AN RNA BASED ASSAY SYSTEM TO
THE DEVELOPMENT OF AN RNA BASED ASSAY SYSTEM TO

... DNA from a victim is found on an implement believed to have been used in a sexual assault, the significance of this evidence would be enhanced by demonstrating that it originated from her vaginal secretions. The final example involves the sexual abuse of a young child by a person living in the same ...
Chapter 1
Chapter 1

... A metabolic pathway is a sequence of enzyme-controlled reactions required to convert substances into useable forms. These pathways are interconnected so that substances can be catabolized or anabolized per the needs of the cells at that particular time. 18. Describe the starting material and product ...
CULTURED DIVERSITY OF ANOXYGENIC PHOTOTROPHIC
CULTURED DIVERSITY OF ANOXYGENIC PHOTOTROPHIC

... thioglycolate were tested for sulfur source. Nitrogen source utilization was tested by replacing ammonium chloride with urea, nitrate, nitrite, glutamate and glutamine at 0.06% (w/v). Vitamin requirement was tested by replacing yeast extract with different vitamins (vitamin B12, biotin, niacin, PABA ...
Protein Targeting
Protein Targeting

... into the aqueous interior of the organelle. • Protein destined for cytosol simply remain where they are synthesized • Mitochondrial and chloroplast proteins are first completely synthesized and released from ribosomes. These are then bound by cytosolic chaperone proteins and delivered to receptor on ...
Fulltext PDF - Indian Academy of Sciences
Fulltext PDF - Indian Academy of Sciences

... had just discovered the secret of life. The April 25 issue of the journal Nature carried the same news in the form of their first, and most famous, paper, "A Structure for Deoxyribose Nucleic Acid". In it they announced that DNA, the molecular basis of heredity, was a right-handed double helix. It c ...
Q1. There are two types of reproduction, asexual and sexual. Use
Q1. There are two types of reproduction, asexual and sexual. Use

... These young rabbits look like their parents. This is because information about characteristics such as fur colour is passed from parents to their young. ...
Macromolecules of the Cell
Macromolecules of the Cell

... transmission and expression of genetic information. They are linear polymers of nucleotides. The two types of nucleic acids are DNA and RNA molecules. DNA molecules serve as the repository of genetic information, whereas RNA has many functions including the expression of that information. Nucleotide ...
Organizing Protein Synthesis - Dallastown Area School District Moodle
Organizing Protein Synthesis - Dallastown Area School District Moodle

... DNA & Protein Synthesis Review Worksheet 1) A _________________________ is a segment of DNA that codes for a protein. 2) __________________________________________ is uncoiled DNA. 3) __________________________________________ is coiled DNA. 4) _________________________________________ is the enzym ...
Oculopharyngeal Muscular Dystrophy
Oculopharyngeal Muscular Dystrophy

... have a strong family history. In some rare cases, OPMD is inherited as an autosomal recessive trait. Here, the defective gene needs to be inherited from both parents. People with a family history of the disease may choose to have genetic counseling to help understand the risks of passing the gene on ...
File
File

... http://www.brooklyn.cuny.edu/bc/ahp/MGInv/M GI.Intro.html ...
Using High-Throughput Sequencing to Investigate the Transgenerational
Using High-Throughput Sequencing to Investigate the Transgenerational

... conditions is high-throughput, next-generation sequencing (NGS) [21]. High-throughput, next-generation ...
MicroReview Expression, secretion and antigenic - UvA-DARE
MicroReview Expression, secretion and antigenic - UvA-DARE

... b. Polycistronic messenger. c. At least one truncated, silent gene is present (Bowditch et al., 1989). d. Based on protein analysis, it is postulated that additional gene(s) are present (Sára and Sleytr, 1994). ORF, open reading frame; n.d., not determined. ...
SCIENCE 9 UNIT A BIOLOGICAL DIVERSITY
SCIENCE 9 UNIT A BIOLOGICAL DIVERSITY

... •Not all organisms have the same number of chromosomes. (A dog cell has 78 chromosomes and cats have 38, so dog cells have __ pairs, and cats have __ pairs). •Not all chromosomes from species to species are the same, which accounts for the different characteristics between _____________. Sex Chromos ...
Document
Document

biochemistry - Bioscience High School
biochemistry - Bioscience High School

... Phospholipids – a phosphate group replaces one fatty acid. Found in cell membranes. Terpenes = pigments such as chlorophyll Prostaglandins = chemical messengers Steroids = parts of hormones ...
Chromosomes and Inheritance - 1 Chromosome Theory of
Chromosomes and Inheritance - 1 Chromosome Theory of

... Chromosome mapping using recombination data was used extensively in the earlier part of the 20th century. Today we have much more sophisticated methods of dealing with chromosome mapping using DNA probes, where a known DNA fragment is used to compare with an unknown chromosome region. If the probe m ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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