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Bacteriophage
Bacteriophage

... Model system of molecular biology Cloning and expression Phage display system Phage typing Phage therapy: ...
Current Uses of Synthetic Biology for Chemicals
Current Uses of Synthetic Biology for Chemicals

... alkane biosynthetic genes were unknown. LS9 designed the pathways, synthesized the genes encoding each enzyme in the pathway, and constructed multigene biosynthetic operons enabling production. To improve yield, productivity, and titer – the drivers of process economic efficiency – the biosynthetic ...
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File

... • Both have various physical and mental changes. ...
COAT AND COLOUR GENES IN DACHSHUNDS
COAT AND COLOUR GENES IN DACHSHUNDS

... DOMINANT to “m”. “Merle” - “Dapple” gene. One parent must be Merle to produce Merle offspring. A “Double-Merle” - ”Double Dapple” (“MM”) can only produce Merle offspring. The mating of two Merles may bring serious problems. ...
Oncogene (2005)
Oncogene (2005)

... morphological changes and inhibits tumorigenesis A549 human lung carcinoma cells were transfected with the full-length TRIT1 transcript and evaluated in vitro for growth characteristics. Real-time quantitative PCR analysis for expression of recombinant vector identified six clones in which TRIT1 mRNA ...
Supplementary Information
Supplementary Information

... 3. Quantitative RT-PCR. Patient muscle RNA was prepared from ~100 mg of vastus lateralis biopsy material by homogenising in 1 ml of QIAzol (RNA extraction kit, QIAGEN) and subjecting to RNA extraction according to the manufacturers’ instructions. Human muscle RNA purchased from Ambion was used as a ...
the smallest dinoflagellate genome is yet to be found: a comment on
the smallest dinoflagellate genome is yet to be found: a comment on

... into the evolution of dinoflagellate genomes and to look for the smallest dinoflagellate genome. What makes the genome of Symbiodinium so ‘‘small’’ and that of some other dinoflagellates so enormous (4200 pg)? As referred to by LaJeunesse et al. (2005), there is a diversity of hypotheses regarding t ...
Phylogenetic relationships among iguanian lizards using alternative
Phylogenetic relationships among iguanian lizards using alternative

srep09383-s1
srep09383-s1

... subsequently ligated into the SpeI and NcoI sites of pDR111, generating pCAPB2 vector. Additionally, the sfp gene flanked by NdeI and XhoI restriction sites was amplified by PCR from genomic DNA of B. subtilis 1779, and the resultant product was introduced into NdeI and XhoI sites in the second mult ...
Faithful meiotic chromosome segregation in Caenorhabditis elegans
Faithful meiotic chromosome segregation in Caenorhabditis elegans

Chromosomal Basis of Inheritance
Chromosomal Basis of Inheritance

... Polyploidy - changes in whole “sets” of chromosomes. ...
SOCIAL STUDIES: Skokie DISTRICT 68
SOCIAL STUDIES: Skokie DISTRICT 68

... How are characteristics of one generation of organisms passed to the next? What are the consequences regarding inheritance and variation across generations? How are the characteristics of one generation of organisms related to the next generation? Why do individuals of the same species vary in how t ...
Microevolution: How Does a Population Evolve?
Microevolution: How Does a Population Evolve?

... – May be without regard to one’s phenotype – Positive assortative mating – choose individuals like ourselves • Inbreeding – increases the incidence of recessive disorders, leading to a less ...
Teacher Quality Grant - Gulf Coast State College
Teacher Quality Grant - Gulf Coast State College

... Students will identify and/or describe the basic molecular structure of carbohydrates, lipids, proteins, and/or nucleic acids. Students will describe the primary functions of carbohydrates, lipids, proteins, and/or nucleic acids in organisms. Items will not refer to intermolecular forces found in th ...
The GOSim package
The GOSim package

... GO terms exist: the ”is-a” relationship and the ”part-of” relationship. Providing a standard vocabulary across any biological resources, the GO enables researchers to use this information for automated data analysis. The GOSim package provides the researcher with various information theoretic simila ...
Analysis by pulsed-field gel electrophoresis mutations in the
Analysis by pulsed-field gel electrophoresis mutations in the

... Summary. A mutagenesis system involving the insertion of a non-transposable antibiotic resistance gene cassette was used to generate stable mutations in the chromosome of Haemophilus influenzae type b strain Eagan. The mutations generated were shown by pulsedfield gel electrophoresis (PFGE) to have ...
Document
Document

... Biopolymers produced via biotechnology are monodisperse; that is, they have precisely defined and controlled chain lengths; on the other hand, it is virtually impossible to produce a monodisperse synthetic polymer. It has recently been shown that polymers with well-defined chain lengths can have unu ...
The cloning and expression characterization of the centrosome
The cloning and expression characterization of the centrosome

... centrosome. So far, little is known about the expression and function of centrosome proteins in spermatogenesis. In this study, we designed the specific primers according to the sequences of human and mouse centrin genes and cloned the cDNA fragments of centrin-1, -2 and –3 from rat testis. The homo ...
Acidaminococcus fermentans type strain (VR4T)
Acidaminococcus fermentans type strain (VR4T)

... The genome was sequenced using a combination of Sanger and 454 sequencing platforms. All general aspects of library construction and sequencing can be found at http://www.jgi.doe.gov/. 454 Pyrosequencing reads were assembled using the Newbler assembler version 2.0.0-PostRelease11/04/2008 (Roche). La ...
Cell biology summary notes [pdf 1MB]
Cell biology summary notes [pdf 1MB]

... protein. Amino acids are held together by peptide bonds. The variety of protein shapes and functions depends on each individual sequence of amino acids. Type of Protein Enzyme Hormones Antibodies Figure 1: Double helix structure of DNA- held together by complimentary base pairs. ...
DNA Before Proteins? Recent Discoveries in
DNA Before Proteins? Recent Discoveries in

... possibility that a ribozyme could perform ribonucleotide reduction through a relatively simple radical-free mechanism. More importantly, the enzymatic route to DNA nucleotides need not have begun with ribonucleotide reduction. The protein enzyme D-2-deoxyribose-5-phosphate aldolase catalyzes the for ...
THE Macromolecules PowerPoint - Panhandle Area Educational
THE Macromolecules PowerPoint - Panhandle Area Educational

... Students will identify and/or describe the basic molecular structure of carbohydrates, lipids, proteins, and/or nucleic acids. Students will describe the primary functions of carbohydrates, lipids, proteins, and/or nucleic acids in organisms. Items will not refer to intermolecular forces found in th ...
Prashanth-Leaflet
Prashanth-Leaflet

... ACI and ACII are called as AC element that mediates transactivation of secondary cell wall biosynthesis. ...
SP7+ P7 (1+3) Energetics and kinetics of chemical reaction.
SP7+ P7 (1+3) Energetics and kinetics of chemical reaction.

... analyze and calculate the basic physicochemical principles that apply to gases and solutions 2. Describe and explain the structure and reactions of the most important biochemical compounds, including small, large and supramolecular structures that Learning outcomes are found in the cell expected at ...
Online Counseling Resource YCMOU ELearning Drive…
Online Counseling Resource YCMOU ELearning Drive…

... Eukaryotes have at least 15 DNA Polymerases:  Pol α : acts as a primase (synthesizing a RNA primer), and then as a DNA Pol elongating that primer with DNA nucleotides.  Pol β: implicated in repairing DNA.  Pol γ: replicates mitochondrial DNA.  Pol δ: main polymerase on the lagging strand, it is ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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