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Macroevolution Part I Phylogenies
Macroevolution Part I Phylogenies

... divided into two groups Archaea and Bacteria. • Organisms are grouped from species to domain, the groupings are increasingly more inclusive. • The taxonomic groups from broad to narrow are domain, kingdom, phylum, class, order, family, genus, and species. • A taxonomic unit at any level of hierarchy ...
lucr_2_georgescu BT
lucr_2_georgescu BT

Life Size Scaling
Life Size Scaling

... A virus’’ only mission is to make more copies of itself. But since it has no reproductive machinery of its own, it invades other organisms and, like a very bad house guest, it sponges off the reproductive resources of its host. Viral genomes are made of DNA or RNA and encode for the proteins needed ...
Selective breeding of corn was originally done by ancient farmers by
Selective breeding of corn was originally done by ancient farmers by

... seem to be suitable, the genotype (and therefore phenotype) of its offspring may not be suitable. If the original plant had a recessive gene in its genotype that was not expressed in its phenotype, and its egg was fertilised with a male pollen grain that also had a recessive gene, there would be a c ...
Breast Cancer
Breast Cancer

... times. The number of repeated sequences in a given STR varies from person to person. The alternate forms of a given STR correspond with different alleles. Most STRs occur in gene introns (non-coding regions of DNA), so the variation in the number of repeats does not usually affect gene function, but ...
Risk assessment of Genetically Modified Micro-Organisms
Risk assessment of Genetically Modified Micro-Organisms

... It is not appropriate to consider non-disabled pathogens of plants, humans, animals or insects as inherently safe recipient micro-organisms. Examples of inherently safe recipient microorganisms which, depending on the nature of the insert, would in most cases be expected to form the basis of extreme ...
A system in mouse liver for the repair of O6
A system in mouse liver for the repair of O6

... To a solution containing 3.1 mg of calf thymus DNA in 1.0 ml of 0.2 M Tris-HCl, pH 8.0 was added 144 u l of 1.0 mCi/ml [3H]-MNU in ethanol. This solution was incubated for 60 min at 37°C, and the DNA was precipitated by the addition of 0.1 vol of 2.5 M sodium acetate, and two volumes of cold ethanol ...
EXAM QUESTIONS PAPER 2 10 SEPTEMBER
EXAM QUESTIONS PAPER 2 10 SEPTEMBER

... What is the probability/chance that they will have a child who will be affected female? ...
Bioinformatics Resources at a Glance A Note about FASTA Format
Bioinformatics Resources at a Glance A Note about FASTA Format

... standard format for nucleotide and protein sequences that allows them to be read by a wide  range of programs. This format is called FASTA format, and each nucleotide or amino acid is  represented using a single letter. The first line of a FASTA is the comment line, identified with  either the great ...
A History of Computing
A History of Computing

... Homology - similarity attributed to descent from a common ancestor. It may or may not result in similar function. Orthologous - homologous sequences in different species that arose from a common ancestral gene. Paralogous - homologous sequences within a single species that arose by gene duplication. ...
Adoption Studies
Adoption Studies

... responses, and environment can affect gene activity. ...
Recombination Frequencies - Western Washington University
Recombination Frequencies - Western Washington University

... • You have to be able to deduce the genotype of the gamete by looking at the phenotype of the offspring, • You must look at enough offspring so that all crossover ...
Homologous Chromosomes
Homologous Chromosomes

... (extra practice with an imaginary creature) ...
Student 3
Student 3

... seem to be suitable, the genotype (and therefore phenotype) of its offspring may not be suitable. If the original plant had a recessive gene in its genotype that was not expressed in its phenotype, and its egg was fertilised with a male pollen grain that also had a recessive gene, there would be a c ...
Handout (Original Version).
Handout (Original Version).

... BACKGROUND: You have just completed an activity in which you made a cladogram showing the evolutionary relationships between seven organisms. The data used to draw that cladogram was based on shared characteristics that were inherited from their ancestors. Biochemical characteristics, like similarit ...
T-Cell Receptor (TCR) Gene Rearrangement, PCR
T-Cell Receptor (TCR) Gene Rearrangement, PCR

... By extracting genomic DNA from blood, lymph node, bone marrow, or other tissue types T-cell receptor gamma and/or T-cell receptor beta are amplified by PCR using a multiplex primer method based on the BIOMED-2 strategy. Precise fragment sizing of the amplicons is accomplished using capillary gel ele ...
Functional Genomics Modeling I
Functional Genomics Modeling I

... AP-1 dependent gene expression ...
Introduction to Cellular and Molecular Biology (BIOL 190)
Introduction to Cellular and Molecular Biology (BIOL 190)

... 4. Describe the structure and basic characteristics of the following functional groups: hydroxyl, carbonyl, carboxyl, amino, sulfhydryl, phosphate, methyl 5. List the four major kinds of biological macromolecules (carbohydrates, proteins, lipids, and nucleic acids); define monomers, polymers, dehydr ...
Protocol for Real-Time RT-PCR - MGH-PGA
Protocol for Real-Time RT-PCR - MGH-PGA

... Little or no PCR product. Poor quality of PCR templates, primers, or reagents may lead to PCR failures. First, please include appropriate PCR controls to eliminate these possibilities. Some genes are expressed transiently or only in certain tissues. In our experience, this is the most likely cause f ...
Klug, A., The discovery of Zinc fingers and Their Application in Gene Regulation and Genome Manipulation . Ann. Rev. Biochem. 79, 213-231 (2010).
Klug, A., The discovery of Zinc fingers and Their Application in Gene Regulation and Genome Manipulation . Ann. Rev. Biochem. 79, 213-231 (2010).

... pair of cysteines and a pair of histidines, and by an inner hydrophobic core. This discovery showed not only a new protein fold but also a novel principle of DNA recognition. Whereas other DNA binding proteins generally make use of the two-fold symmetry of the double helix, zinc fingers can be linked ...
Carbohydrates
Carbohydrates

... Examples of disaccharides: - maltose (two glucoses) - sucrose (a glucose and fructose) - lactose (a glucose and galactose) To break the bond between two sugars, a H2O is needed (Hydrolysis) The Main Functions of Carbohydrates are: Energy: ...
Back-translation Using First Order Hidden Markov Models
Back-translation Using First Order Hidden Markov Models

... of the same species of plant), we expected a minuscule error rate in our backtranslated output in each of the three scenarios. Further, we predicted that the Spanish training set would give the most accurate back-translation of the three sets and the Russian set would give the least accurate. Surpr ...
Chirality in Chemistry
Chirality in Chemistry

... Examples of secondary structures can be found here. Why does the shape of this secondary structure matter? As enzymes, the biological catalysts which allow our cells to work, are made of proteins, the shape of the secondary structure is important in how they can function. Enzymes work through a “lo ...
Mendelian Genetics by Dr. Ty C.M. Hoffman
Mendelian Genetics by Dr. Ty C.M. Hoffman

... tracked  at  the  same  time.  If  there  were  no  independent  assortment,  then  the  alleles  for  the  two  genes   from  one  parent  would  always  remain  together.  This  would  result  in  offspring  that  are  entirely  lik ...
What are mitochondria? Mitochondria are the
What are mitochondria? Mitochondria are the

... We presented a forensic case based on synthetic biology, as well as teaching students about common techniques used in the lab and allowed them to crack the case themselves using what they had learned. ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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